G protein alpha 12 (GNA12) (NM_007353) Human Untagged Clone

CAT#: SC115556

GNA12 (untagged)-Human guanine nucleotide binding protein (G protein) alpha 12 (GNA12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: G protein alpha 12
• Synonyms: gep; NNX3; RMP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007353, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCGGGGTGGTGCGGACCCTCAGCCGCTGCCTGCTGCCGGCCGAGGCCGGCGGGGCCCGCGAGCGCA
  GGGCGGGCAGCGGCGCGCGCGACGCGGAGCGCGAGGCCCGGAGGCGTAGCCGCGACATCGACGCGCTGCT
  GGCCCGCGAGCGGCGCGCGGTCCGGCGCCTGGTGAAGATCCTGCTGCTGGGCGCGGGCGAGAGCGGCAAG
  TCCACGTTCCTCAAGCAGATGCGCATCATCCACGGCCGCGAGTTCGACCAGAAGGCGCTGCTGGAGTTCC
  GCGACACCATCTTCGACAACATCCTCAAGGGCTCAAGGGTTCTTGTTGATGCACGAGATAAGCTTGGCAT
  TCCTTGGCAGTATTCTGAAAATGAGAAGCATGGGATGTTCCTGATGGCCTTCGAGAACAAGGCGGGGCTG
  CCTGTGGAGCCGGCCACCTTCCAGCTGTACGTCCCGGCCCTGAGCGCACTCTGGAGGGATTCTGGCATCA
  GGGAGGCTTTCAGCCGGAGAAGCGAGTTTCAGCTGGGGGAGTCGGTGAAGTACTTCCTGGACAACTTGGA
  CCGGATCGGCCAGCTGAATTACTTTCCTAGTAAGCAAGATATCCTGCTGGCTAGGAAAGCCACCAAGGGA
  ATTGTGGAGCATGACTTCGTTATTAAGAAGATCCCCTTTAAGATGGTGGATGTGGGCGGCCAGCGGTCCC
  AGCGCCAGAAGTGGTTCCAGTGCTTCGACGGGATCACGTCCATCCTGTTCATGGTCTCCTCCAGCGAGTA
  CGACCAGGTCCTCATGGAGGACAGGCGCACCAACCGGCTGGTGGAGTCCATGAACATCTTCGAGACCATC
  GTCAACAACAAGCTCTTCTTCAACGTCTCCATCATTCTCTTCCTCAACAAGATGGACCTCCTGGTGGAGA
  AGGTGAAGACCGTGAGCATCAAGAAGCACTTCCCGGACTTCAGGGGCGACCCGCACAGGCTGGAGGACGT
  CCAGCGCTACCTGGTCCAGTGCTTCGACAGGAAGAGACGGAACCGCAGCAAGCCACTCTTCCACCACTTC
  ACCACCGCCATCGACACCGAGAACGTCCGCTTCGTGTTCCATGCTGTGAAAGACACCATCCTGCAGGAGA
  ACCTGAAGGACATCATGCTGCAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007353 unedited
  GCCAGCGAATTCGGCACGAGGCGGGGCGTGGGGCGGTGCCTCGGCCCGGGCTCGCCCTCG
  CCGGCGGGAGCGTCCATGGCCCCCGGGCGCCGGCGGGGCGCGGCCGCGGCCTGAGGGGCC
  ATGTCCGGGGTGGTGCGGACCCTCAGCCGCTGCCTGCTGCCGGCCGAGGCCGGCGGGGCC
  CGCGAGCGCAGGGCGGCATCGGCGCGCGCGACGCGGAGCGCGAGGCCCGGAGGCGTAGCC
  GCGACATCGACGCGCTGCTGGCCCGCGAGCGGCGCGCGGTCCGGCGCCTGGTGAAGATCC
  TGCTGCTGGGCGCGGGCGAGAGCGGCAAGTCCACGTTCCTCAAGCAGATGCGCATCATCC
  ACGGCCGCGAGTTCGACCAGAAGGCGCTGCTGGAGTTCCGCGACACCATCTTCGACAACA
  TCCTCAAGGGCTCAAGGGTTCTTGTTGATGCACGAGATAAGCTTGGCATTCCTTGGCAGT
  ATTCTGAAAATGAGAAGCATGGGATGTTCCTGATGGCCTTCGAGAACAAGGCGGGGCTGC
  CTGTGGAGCCGGCCACCTTCCAGCTGTACGTCCCGGCCCTGAGCGCACTCTGGAGGGATT
  CTGGCATCAGGGAGGCTTTCAGCCGGAGAAGCGAGTTTCACCTGGGGGAGTCGGTGAAGT
  ACTTCCTGGACAACTTGGACCGGATCGGCCAGCTGAATTACTTTTCCTATAAGCAAGATA
  TCCTGCTTGCTAAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007353 unedited
  NNNNNGGGTATTGCTATGGAACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTT
  TTTTTTTTTTAAGCAAAGAAAGACTAATTTATTTTAATAAACATTCAGAGGCTGAGGGTG
  GCCAAATTTTCAAGCCAGACCCTCCCAATGTTAATACACTGTGCAAAGCTTACACCATGA
  ACAACTGACCCGGACCACTACCATTCCAATGGGAGCCTCTGCCTTACAATACCAGGTTAG
  ATGTCTGTGCCCAGAACCCCCAAATTAGTTACAACTAATAATCTTCTTTCAAGAGTTTTT
  TTTTTCAATCTTGGAGTTAACAATCTGACTTATTTTTCTTTTATCAAAGAAGTAGGAAAA
  TTACAATAAGAATTCTAGAGATCTCTATAAATTACAGACCTATGACTCAAAATCCTTGAA
  ACAATTTCTCTACGAACATAAGAGTTAAAAATAGATTTCAGTAAAACCCTATATGCGTCT
  TATGTGTACACAGTATAGCTATGAGGAACAGATCTTATTTTGAGGAACTTTATTGTTACA
  TTTTTGCAATAATTGAGGCACAACTACCTCCTGCTTTTCCAACATCTTAATAAAAAAGAC
  AATAAGGATTAACAGTGAAATTAAAATTAAAAAATACAAAAGCCTAAGGTTCTGGAGACA
  AAACTGACTAGAGTCTATGTGTAGCCAAGTTGTGAATGACAGTTTAGCCTTGCAGAGTTT
  CCTTCTTCTCCATTACAATGTGTTACAGAATTTGAAGGGGGGTGTCTTTAAAAACGTCTA
  TTCACCGGTCCTTGAGGAGAGAGGGGGGGAATCCCCCTTANCAGGCCAGTGCCTCGCCCG
  GGCGGTGGGGTCACCGCTGAACGGTCAAGACCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007353
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007353.2, NP_031379.2
• RefSeq Size: 4398 bp
• RefSeq ORF: 1146 bp
• Locus ID: 2768
• UniProt ID: Q03113
• Cytogenetics: 7p22.3-p22.2
• Domains: G-alpha
• Protein Families: Druggable Genome
• Protein Pathways: Long-term depression, MAPK signaling pathway, Regulation of actin cytoskeleton, Vascular smooth muscle contraction
• Gene Summary: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:22609986, PubMed:15525651, PubMed:15240885, PubMed:17565996, PubMed:12515866, PubMed:16787920, PubMed:16705036, PubMed:23762476, PubMed:27084452). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG) (PubMed:15240885, PubMed:12515866, PubMed:16202387). GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (By similarity). GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins (PubMed:15525651, PubMed:17565996). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating proinflammatory cytokine production (PubMed:23762476, PubMed:16787920, PubMed:16705036, PubMed:27084452). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (PubMed:11976333, PubMed:16787920). Together with NAPA promotes CDH5 localization to plasma membrane (PubMed:15980433). May play a role in the control of cell migration through the TOR signaling cascade (PubMed:22609986).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).