HS2ST1 (NM_012262) Human Untagged Clone

CAT#: SC115469

HS2ST1 (untagged)-Human heparan sulfate 2-O-sulfotransferase 1 (HS2ST1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HS2ST1
• Synonyms: dJ604K5.2; NFSRA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_012262 edited
  ATGGGGCTCCTCAGGATTATGATGCCGCCCAAGTTGCAGCTGCTGGCGGTGGTGGCCTTC
  GCGGTGGCGATGCTCTTCTTGGAAAACCAGATCCAGAAACTGGAGGAGTCCCGCTCGAAG
  CTAGAAAGGGCTATTGCAAGACACGAAGTCCGAGAAATTGAGCAGCGACATACAATGGAT
  GGCCCTCGGCAAGATGCCACTTTAGATGAGGAAGAGGACATGGTGATCATTTATAACAGA
  GTTCCCAAAACGGCAAGCACTTCATTTACCAATATCGCCTATGACCTGTGTGCAAAGAAT
  AAATACCATGTCCTTCATATCAACACTACCAAAAATAATCCAGTGATGTCATTGCAAGAT
  CAGGTGCGCTTTGTAAAGAATATAACTTCCTGGAAAGAGATGAAACCAGGATTTTATCAT
  GGACACGTTTCTTACTTGGATTTTGCAAAATTTGGTGTGAAGAAGAAACCAATTTACATT
  AATGTCATAAGGGATCCTATTGAGAGGCTAGTTTCTTATTATTACTTTCTGAGATTTGGA
  GATGATTATAGACCAGGGTTACGGAGACGAAAACAAGGAGACAAAAAGACCTTTGATGAA
  TGTGTAGCAGAAGGTGGCTCAGACTGTGCTCCAGAGAAGCTCTGGCTTCAAATCCCGTTC
  TTCTGTGGCCATAGCTCCGAATGCTGGAATGTGGGAAGCAGGTGGGCTATGGATCAAGCC
  AAGTATAACCTAATTAATGAATATTTTCTGGTGGGAGTTACTGAAGAACTTGAAGATTTT
  ATCATGTTATTGGAGGCAGCATTGCCCCGGTTTTTCAGGGGTGCTACTGAACTCTATCGC
  ACAGGAAAGAAATCTCATCTTAGGAAAACCACAGAGAAGAAACTCCCCACTAAACAAACC
  ATTGCAAAACTACAGCAATCTGATATTTGGAAAATGGAGAATGAGTTCTATGAATTTGCA
  CTAGAGCAGTTCCAATTCATCAGAGCCCATGCCGTTCGAGAAAAAGATGGAGACCTCTAC
  ATCCTCGCACAAAACTTTTTCTATGAAAAGATTTACCCTAAGTCGAACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012262 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTTCATGGGNGCTC
  CTCAGGATTATGATGCCGCCCAAGTTGCAGCTGCTGGCGGTGGTGGCCTTCGCGGTGGCG
  ATGCTCTTCTTGGAAAACCAGATCCAGAAACTGGAGGAGTCCCGCTCGAAGCTAGAAAGG
  GCTATTGCAAGACACGAAGTCCGAGAAATTGAGCAGCGACATACAATGGATGGCCCTCGG
  CAAGATGCCACTTTAGATGAGGAAGAGGACATGGTGATCATTTATAACAGAGTTCCCAAA
  ACGGCAAGCACTTCATTTACCAATATCGCCTATGACCTGTGTGCAAAGAATAAATACCAT
  GTCCTTCATATCAACACTACCAAAAATAATCCAGTGATGTCATTGCAAGATCAGGTGCGC
  TTTGTAAAGAATATAACTTCCTGGAAAGAGATGAAACCAGGATTTTATCATGGACACGTT
  TCTTACTTGGATTTTGCAAAATTTGGTGTGAAGAAGAAACCAATTTACATTAATGTCATA
  AGGGATCCTATTGAGAGGCTAGTTTCTTATTATTACTTTCTGAGATTTGGAGATGATTAT
  AGACCAGGGTTACGGAGACGAAAACAAGGAGACANAAAGACCTTTGATGAATGTGTAGCA
  GAAGGTGGCTCAGACTGTGCTCCAGAGAAGCTCTGGCTTCAAATCCCGTTCTTCTGTGGC
  CATAGCTCCGAATGCTGGAATGTGGGAAGCANGTGGGCTATGGNATCAAGCCAGTATAAC
  CTAATAATGAATATTNTCTGGTGGGGAGTACTGAAGAAACTTGAGATTTATCATGGTATT
  GGNAGGCAGCATGCCCCCGTTTTTCAGGGGTGCTACTGAACTCTATCGACAGGAAAGAAT
  CTCATCTANGAAACCACAGAGAGAAACTCCCACTAACAACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012262 unedited
  NNTTTCTCTGGACCCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTATTTA
  TCAAACACAAATTTATATCAAGATTAACTTTTTCAACCAGCTTACAAGCAGACTTTTCCC
  TTTCTTTGTTAAAAAATGAATGGTATGAAACTATATGTTAAAGATGTTTCTATAATACCT
  TACATTTACATAGCGCTTTACAATTTTCAAAGTGCTTTCACATGCCATCTCATTTAATCC
  TCACAACAGCCCTGTGAGGTAGGTACAGCAGGTATTATTATCCTCATTTCAAGATGAGGA
  AATTGAGGCAAAGAGAGGTTAAGTGACTTGCCCAAGATCACACAGCTGTAAGTGCTAGAG
  CTAGGACCTGAAATCAAGTTTTTTGACTCCTTGTCCAGTACTCATTCCACTGTAATTACT
  GCCTCAAGTTATGAATAATGAACTGTGTATCAAAATTGAAAGCTTACTGAAGTTCATTTC
  AGTGATGTCTTAACAGTAAAGTTAAATGAGAAATACAAGAACAGTAGGTCTGATGGTTTT
  ACTTGACATAAAATTGGTGCAAAACCACTTTTGTCTAATTACTTGTAACCTTTTGGTCCA
  AATACATAAAATATGCAATATTTACAGCATTTTCCTTGTTTTGTAAATGCAATGCTTTAA
  AACACATTATAAAGCACCTTAGTAAAAATAGTCTACAGGTAAAAGTACAGAAGAAGTANG
  GAAAATTCTACCCATACCACAGCTCACTAATGGAGTCATAATACCGAGTCACCGATATCT
  CAGTTTACAGCTTTACAAATTACCCATGGAGAAACAATACTAAACTTACTTTTTTTGAAA
  TAAAGGGGGAAAAACCTAACTAAAACCTCAGACAATCTCAATATTCCTTTGAGAATTCTT
  CCCCATTTTAAAAATTGAGGGAGGCACGAATGAGAAAAACCGCGTTTGAGCGCTTAACTT
  TTTTACAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012262
• Insert Size: 2450 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012262.2, NP_036394.1
• RefSeq Size: 6708 bp
• RefSeq ORF: 1071 bp
• Locus ID: 9653
• UniProt ID: Q7LGA3
• Cytogenetics: 1p22.3
• Domains: HS2ST
• Protein Pathways: Heparan sulfate biosynthesis
• Gene Summary: Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Aug 2008]
  Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.