HYAL4 (NM_012269) Human Untagged Clone

CAT#: SC115433

HYAL4 (untagged)-Human hyaluronoglucosaminidase 4 (HYAL4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HYAL4
• Synonyms: CSHY; HYAL-4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115433 sequence for NM_012269 edited (data generated by NextGen Sequencing)
  ATGAAAGTATTATCTGAAGGACAGTTAAAGCTTTGTGTTGTTCAACCAGTACATCTCACT
  TCATGGCTCCTTATATTTTTTATTCTAAAGTCTATCTCTTGTCTAAAACCTGCTCGACTT
  CCAATTTATCAAAGGAAACCTTTTATAGCTGCTTGGAATGCTCCAACAGATCAGTGTTTG
  ATAAAATATAATTTAAGACTAAATTTGAAAATGTTTCCTGTGATTGGAAGCCCACTGGCC
  AAGGCCAGGGGGCAAAATGTCACTATATTTTATGTCAACAGATTGGGATACTATCCGTGG
  TATACATCACAAGGGGTCCCCATTAATGGAGGTCTCCCACAGAACATAAGTTTACAAGTA
  CATCTGGAAAAAGCTGACCAAGATATTAATTATTACATCCCTGCTGAAGATTTCAGTGGA
  CTTGCTGTTATAGATTGGGAATATTGGCGACCACAGTGGGCCCGGAACTGGAACTCAAAA
  GATGTTTACAGACAGAAGTCAAGAAAGCTTATTTCCGATATGGGAAAGAATGTATCAGCT
  ACCGATATTGAATATTTAGCCAAAGTGACCTTTGAAGAAAGTGCAAAAGCTTTCATGAAG
  GAAACCATCAAATTGGGAATTAAGAGCCGACCCAAAGGCCTTTGGGGTTATTATTTATAT
  CCTGATTGCCACAATTATAACGTTTATGCCCCAAACTACTCTGGGTCATGCCCAGAAGAC
  GAAGTCTTGAGGAACAATGAGCTCTCTTGGCTCTGGAACAGCAGTGCTGCTTTATATCCT
  TCTATCGGTGTCTGGAAATCCCTTGGAGACAGTGAAAACATTTTGCGCTTCTCCAAATTT
  CGGGTGCATGAATCCATGAGGATCTCCACCATGACATCTCATGATTATGCTCTGCCTGTA
  TTTGTCTACACAAGGCTAGGGTACAGAGATGAACCTTTATTTTTCCTTTCTAAGCAAGAT
  CTAGTCAGCACCATAGGAGAAAGTGCTGCCTTGGGAGCTGCAGGCATTGTTATTTGGGGA
  GACATGAATTTAACTGCATCCAAGGCCAACTGTACAAAGGTGAAGCAGTTTGTGAGTTCT
  GATTTAGGGAGCTACATAGCCAATGTGACCAGAGCTGCTGAGGTATGCAGCCTTCACCTC
  TGCAGGAACAATGGCAGGTGCATAAGGAAGATGTGGAACGCGCCCAGTTACCTTCACTTG
  AACCCTGCAAGTTACCACATAGAGGCCTCTGAGGACGGGGAGTTTACTGTGAAAGGAAAA
  GCATCTGATACAGACCTGGCAGTGATGGCAGATACATTTTCCTGTCATTGTTATCAGGGA
  TATGAAGGAGCTGATTGCAGAGAAATAAAGACGGCTGATGGCTGCTCTGGGGTTTCCCCT
  TCTCCTGGTTCACTAATGACACTTTGTCTACTGCTTTTAGCAAGTTATCGAAGCATTCAG
  TTGTGA
  
  Clone variation with respect to NM_012269.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012269 unedited
  GGGGGGGNNNNNNNNCTTANNNNNNNGGTTTAAATTTGTATACAATTATATAGGCGGCCG
  CGAAATTCGCACCAGCCCTCCCACCTTCCCGCAAGCTGAGGGAGCCGGCTCTGGCCTTGG
  CCAGTCCAGAAAGGGGCTCCCACAGTGCAGCGGCGGGCTGATGGGCTCCTCAAGCGCGGC
  CAGAGTGGGCGCCAAGGCCGAGGAGGCGCCGAGAGCGAGCGAGGGCTGCGAGGACTGCCA
  GCATGCTGTCACCTCTCAATGTCAGCTGAGGAGAGGAAAGGATTCTTAGCTTGAGTTCAC
  TCCAGTTGCCTAATGTCATGCCCATTGCTCAAGCCCATGTGGCCTGTTTGAAGTCAGGTC
  CCTCAGCTGCAGGTCTGTTGGAGTTTGCTGGAGGTCCATTCCAGACCTTGTTTACCTGGG
  TATCACCAGCGGAGGCTGCAGAACCGCAAGAAAATCAGGAATTTCTTCTTGGTTTGGAGC
  CATTGCTGGACATCCTTTGCCATTCAACCTCTGATTTGCACAAGGTGACTAAAGGACCAG
  CAGCAAACAAAAGCAAAGGTCTTCTAGAGTGCACTAAAGCAGAAGATAACGTAACATTNT
  TATCTTACCATGAAAGTATTATCTGAAGGACAGTTAAAGCTNTGTGTTGTTCAACCAGTA
  CATCTCACTTCATGGCTCCTTATATNNTTTATTCTAAAGNCTATCTCTTGTCTAAAACCT
  GCTCGACTTCCAAATTATCANAGGGAACCTTNTATAGCTGCTTGGAATGCTNNNCACAGA
  TCAGTGTTTTGATAAAATATATTTAAGACTAANNNTTGAAATGNTTNCTGTGATGGAAGC
  CCACTGGGCCAGGCCAGGGGCAAATGTCACTATATTTATGTCACAGATNGGAATCTATNC
  GTGTATACATCACAAGGGGTCCCATTATGGNAGTCTCCACAGACATAGTTTAAAGTACTC
  TGAAAAGCTGACAGATATTATATACTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012269 unedited
  NNAACGTACAACTATGNACCGCGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTGA
  AATGAAAATTTAATATTTTTATGTATAATTTTATGTATGTACATTCATGAAAAGACTAAA
  TTATACTAGTTTTAGTTTAAATGCAACTTAAATATTCCAATAAGGAGGAAGTGTACATTG
  ACTTTACCCCAAATCTGGTTTCCTAGCCAGACTGGAGGCAAATAAAATAATGTTTCTGTT
  TATGTTAAGTGACATACATAATGTCTACTTATAATATCTCTCAATAGAATTCATAAGAGA
  AAAAAAATGTTATAAGTTACATCCTTCTTTAAATGACTAGGCTAGAGGCCACACAATTCC
  CTTTAAACTCAATTATCTCACAACTGAATGCTTCGATAACTTGCTAAAAGCAGTAGACAA
  AGTGTCATTAGTGAACCAGGAGAAGGGGAAACCCCAGAGCAGCCATCAGCCGTCTTTATT
  TCTCTGCAATCAGCTCCTTCATATCCCTGATAACAATGACAGGAAAATGTATCTGCCATC
  ACTGCCAGGTCTGTATCAAATGCTTTTCCTTTCACAGTAAACTCCCCGTCCTCAGAGGCC
  TCTATGTGGTAACTTGCAGGGTTCAAGTGAAGGTAACTGGGCGCGTTCCACATCTTCCTT
  ATGCACCTGCCATTGTTCCTGCAGAGGTGAAGGCTGCATACCTCAGCAGCTCTGGTCACA
  TTGGCTATGTAGCTCCCTAAATCAGAACTCACAAACTGCTTTCACTTTGTACAGTTGGCT
  TACCTTGGATGCAGTTAAATTCATGTCTCCCCAAATAACATGCCTGCAGCTCCCAAGCAG
  CACTTTCTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012269
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012269.1, NP_036401.1
• RefSeq Size: 2414 bp
• RefSeq ORF: 1446 bp
• Locus ID: 23553
• UniProt ID: Q2M3T9
• Cytogenetics: 7q31.32
• Domains: Glyco_hydro_56
• Protein Pathways: Glycosaminoglycan degradation, Metabolic pathways
• Gene Summary: This gene encodes a protein which is similar in structure to hyaluronidases but lacks hyaluronidase activity. The encoded protein acts as a chondroitin-sulfate-specific endo-beta-N-acetylgalactosaminidase; that is, it exhibits hydrolytic activity toward chondroitin sulfate chains and degrades them into oligosaccharides. Proteoglycans are formed by the covalent linkage of chondroitin sulfate chains to protein. Proteoglycans are ubiquitous components of the extracellular matrix of connective tissues and are also found at the surface of many cell types where they participate in a variety of cellular processes such as cell proliferation, differentiation, migration, cell-cell recognition, extracellular matrix deposition, and tissue morphogenesis. The expression of this gene is highest in testes and placenta. [provided by RefSeq, Apr 2019]