ATP6V0A2 (NM_012463) Human Untagged Clone

CAT#: SC115366

ATP6V0A2 (untagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP6V0A2
• Synonyms: A2; a2V; ARCL; ARCL2A; ATP6A2; ATP6N1D; J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115366 sequence for NM_012463 edited (data generated by NextGen Sequencing)
  ATGGGGTCCCTGTTCCGGAGCGAGACCATGTGCCTGGCGCAGCTCTTCCTGCAGTCGGGC
  ACGGCCTACGAGTGCCTCAGCGCCCTGGGCGAGAAAGGCCTGGTCCAGTTCCGAGACCTC
  AACCAGAACGTAAGTTCTTTCCAAAGAAAATTTGTTGGTGAGGTGAAGAGGTGTGAAGAG
  CTAGAGCGAATATTGGTGTATTTGGTACAGGAAATTAATAGAGCTGATATTCCCCTTCCT
  GAAGGAGAGGCCAGCCCTCCTGCGCCACCCCTGAAACAGGTTCTAGAAATGCAGGAGCAG
  TTGCAGAAGCTCGAGGTTGAACTGAGAGAAGTCACTAAGAACAAGGAGAAACTGAGGAAA
  AACTTGCTGGAACTGATAGAGTACACTCACATGCTGAGAGTGACAAAGACCTTTGTGAAA
  CGCAACGTTGAGTTTGAACCCACTTATGAAGAATTCCCTTCCTTAGAGAGCGATTCTTTG
  TTGGATTACAGCTGTATGCAGAGGCTGGGAGCAAAACTGGGATTTGTGTCTGGCCTAATT
  AACCAAGGAAAAGTGGAAGCATTTGAAAAAATGTTGTGGAGAGTCTGCAAAGGGTACACC
  ATCGTGTCCTATGCAGAACTGGATGAATCCCTTGAAGACCCTGAAACAGGGGAAGTCATA
  AAATGGTATGTCTTTTTAATATCCTTTTGGGGAGAGCAGATTGGCCACAAGGTTAAGAAG
  ATATGTGATTGCTACCACTGCCACGTGTACCCCTATCCAAACACAGCCGAGGAGCGGAGG
  GAGATCCAGGAGGGGCTGAACACCCGCATCCAGGATCTCTACACTGTACTGCACAAAACC
  GAGGACTATTTGAGGCAAGTGCTATGTAAAGCCGCCGAGTCTGTCTACAGCCGTGTGATC
  CAGGTGAAGAAAATGAAGGCCATCTATCACATGCTGAACATGTGCAGCTTTGACGTGACC
  AACAAGTGCCTCATTGCTGAGGTCTGGTGTCCCGAGGCGGATCTGCAGGACCTGCGCCGG
  GCACTGGAGGAGGGCTCGAGAGAGAGTGGTGCTACAATCCCCTCATTCATGAATATAATC
  CCCACAAAAGAAACACCCCCCACTCGGATCCGCACCAACAAATTCACCGAGGGATTTCAG
  AACATCGTGGATGCTTATGGAGTCGGAAGCTACAGAGAAGTCAATCCAGCTCTCTTTACC
  ATCATCACCTTCCCGTTTTTATTTGCTGTGATGTTTGGAGACTTCGGACATGGCTTTGTG
  ATGTTTTTATTTGCCCTCTTGTTGGTGTTAAATGAAAATCATCCCAGACTAAATCAGTCA
  CAAGAGATCATGAGGATGTTTTTTAATGGCCGGTACATCCTCCTGCTGATGGGGCTGTTC
  TCAGTGTACACTGGCCTCATCTACAACGACTGCTTTTCAAAGTCAGTCAACCTGTTCGGC
  TCTGGGTGGAACGTGTCGGCCATGTACAGCTCCAGCCACCCACCCGCAGAGCATAAGAAG
  ATGGTGCTTTGGAACGACAGCGTCGTTAGACACAACAGCATTTTGCAGCTGGATCCAAGC
  ATTCCTGGAGTGTTCCGAGGCCCTTATCCCCTTGGCATTGATCCTATTTGGAACTTGGCC
  ACAAATCGCCTCACTTTTCTAAACTCTTTCAAAATGAAAATGTCCGTGATTTTAGGAATC
  ATTCATATGACTTTTGGAGTCATTCTGGGAATATTTAACCACTTGCACTTCAGGAAGAAG
  TTCAACATTTACCTGGTTTCCATCCCGGAACTTCTCTTCATGCTCTGTATCTTTGGATAC
  CTTATATTTATGATTTTCTACAAGTGGCTGGTTTTTTCAGCAGAAACCTCCAGAGTTGCT
  CCCAGCATTCTGATTGAATTTATTAACATGTTTTTATTCCCAGCCAGTAAAACAAGTGGC
  CTTTACACAGGGCAGGAGTATGTCCAGAGAGTGCTGCTGGTTGTCACAGCATTGTCTGTC
  CCTGTCCTCTTCTTGGGAAAGCCACTGTTTTTGTTGTGGCTTCACAATGGGCGTAGTTGC
  TTCGGGGTGAACCGGAGTGGCTACACACTTATAAGGAAAGATAGTGAGGAAGAAGTTTCA
  TTGCTGGGAAGCCAAGATATAGAAGAGGGAAATCACCAGGTGGAAGATGGATGTAGAGAA
  ATGGCGTGTGAAGAGTTTAATTTTGGAGAAATATTAATGACCCAAGTAATCCATTCCATC
  GAGTACTGTCTGGGATGCATCTCCAACACCGCCTCCTACCTGAGGCTCTGGGCGCTTAGC
  CTGGCTCACGCACAGTTGTCTGATGTCCTGTGGGCCATGCTGATGCGCGTGGGCCTCCGC
  GTTGACACCACCTATGGCGTCTTGCTACTGCTCCCGGTTATCGCGCTCTTTGCAGTTTTG
  ACCATTTTCATCCTTCTGATCATGGAAGGGCTTTCTGCGTTTCTTCACGCCATACGCCTC
  CACTGGGTAGAATTTCAGAACAAATTCTACGTTGGTGCAGGCACCAAATTTGTTCCTTTC
  TCATTCAGTCTACTTTCATCAAAGTTCAATAACGACGACAGTGTGGCATGA
  
  Clone variation with respect to NM_012463.3
  426 t=>c;471 t=>c;1515 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012463 unedited
  TCAAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGCGGACTGC
  TGTGGCGGCAGCTGGAGCGGCGGCCGCGGTGGCAGAACCGGGGGCGGCCGCTGCAGTCTG
  GAGCCCCATAGTGCGGGGCCGCGGCCAGGCCACAGGAAGAGCTCGAGGCCCGGGCCGCAC
  CGGCTGAGTGTGCGGGCCCGCGCGGCTCGGAGCCGCCGCCGCCCATCGAGCCCCTCCGGG
  CGCGGGTCGGCCCGCCATGGGGTCCCTGTTCCGGAGCGAGACCATGTGCCTGGCGCAGCT
  CTTCCTGCAGTCGGGCACGGCCTACGAGTGCCTCAGCGCCCTGGGCGAGAAAGGCCTGGT
  CCAGTTCCGAGACCTCAACCAGAACGTAAGTTCTTTCCAAAGAAAATTTGTTGGTGAGGT
  GAAGAGGTGTGAAGAGCTAGAGCGAATATTGGTGTATTTGGTACAGGAAATTAATAGAGC
  TGATATTCCCCTTCCTGAAGGAGAGGCCAGCCCTCCTGCGCCACCCCTGAAACAGGTTCT
  AGAAATGCAGGAGCAGTTGCAGAAGCTCGAGGTTGAACTGAGAGAAGTCACTAAGAACAA
  GGAGAAACTGAGGAAAAACTTGCTGGAACTGATAGAGTACACTCACATGCTGAGAGTGAC
  AAAGACCTTTGTGAAACGCAACGTTGAGTTTGAACCCACTTATGAAGAATTCCCTTCCTT
  AGAGAGCGATTCTTTGTTGGATTACAGCTGTATGCAGNAGCTGGGAGCAAAACTGGATTT
  GTGTCTGGCCTAATAACCAAGGAAAGTGGAGCATTGAAAAATGTTGTGGAGAGTCTNGCA
  AGGTACACCATCGTGTCTATGCAAACTGGATGATCCCTTGAGACCTGAACAGGGGAGTCA
  TAAGGNATGTCTTTTAATATCCTTTGGGAGACAGATGGCCACAGGTAGAAGATTGTGATG
  CTAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012463 unedited
  NNTTCGGTACACCTAAGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTT
  TTTTTTTTTTTCATGGAAAAATATCTTCATTTATATTTTGTTACAAAAATATACAAAATA
  CTGCTTTTCACCTTTCCTTCACTCAGTATAAACCCTTCTGGTTCAGTGACATCAGCCAAG
  CTGTCACTAAATTCAAATCATGTCCGCATTATATCTTCTACAGTCACACTGGGTTTTAAG
  GCTCAGCATACAATTTATCTTCCAAACATACAATTATCATAACTGGTTTTATGTAAGGGT
  AACAGGATAAGAGTCTTTAAAAAAATATACAAGTGGGATAACCATTTGTTGTATGTCATT
  TTATCCCATTGTCCCCAATTACTAAAACAAGACAAAATTGTAAAAACAATCCTCTACTTG
  TAATTCTAGGTAGCACAGGATAAAAAATATTTGCATATTTTGGCCAGGCATGGTGGCTCA
  CGCCTGTAATCCCAGCACTTAGGGAGGCCGAGGCAGGTGGATCACTTGAGGTCAGAAGTT
  CGAGACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACAAAAATACAAAAAAATTAGC
  TGGTCACGGTGGCGCATGCCTGTAATCCCAGTTACTCGGGAGGCTGAGACAGGAGAATCA
  CCTGAATCCAGGAGGCGGAAGTTGCAATGAGACACGATCGTGCCATTGCACTCCAGCCTA
  CGTGACACAGCAAGACTTCATCTCAAAAAAAAAAAAAGTACATATTTTTAAATCCAGCAG
  ATAAATGTGAAATGTTTTGATTACCCATCATTAAATTTTTTAAAAAACTATCCCTACTTC
  AGCTGTGGNAAAAATTATCTGGCAACTTCAGGTGACAGATAACTCTGTTGTTTGGTTTAA
  AAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012463
• Insert Size: 4540 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012463.2, NP_036595.2
• RefSeq Size: 4681 bp
• RefSeq ORF: 2571 bp
• Locus ID: 23545
• UniProt ID: Q9Y487
• Cytogenetics: 12q24.31
• Domains: V_ATPase_sub_a
• Protein Families: Transmembrane
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Gene Summary: The protein encoded by this gene is a subunit of the vacuolar ATPase (v-ATPase), an heteromultimeric enzyme that is present in intracellular vesicles and in the plasma membrane of specialized cells, and which is essential for the acidification of diverse cellular components. V-ATPase is comprised of a membrane peripheral V(1) domain for ATP hydrolysis, and an integral membrane V(0) domain for proton translocation. The subunit encoded by this gene is a component of the V(0) domain. Mutations in this gene are a cause of both cutis laxa type II and wrinkly skin syndrome. [provided by RefSeq, Jul 2009]