GABRP (NM_014211) Human Untagged Clone

CAT#: SC115134

GABRP (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, pi (GABRP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GABRP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115134 sequence for NM_014211 edited (data generated by NextGen Sequencing)
  ATGAACTACAGCCTCCACTTGGCCTTCGTGTGTCTGAGTCTCTTCACTGAGAGGATGTGC
  ATCCAGGGGAGTCAGTTCAACGTCGAGGTCGGCAGAAGTGACAAGCTTTCCCTGCCTGGC
  TTTGAGAACCTCACAGCAGGATATAACAAATTTCTCAGGCCCAATTTTGGTGGAGAACCC
  GTACAGATAGCGCTGACTCTGGACATTGCAAGTATCTCTAGCATTTCAGAGAGTAACATG
  GACTACACAGCCACCATATACCTCCGACAGCGCTGGATGGACCAGCGGCTGGTGTTTGAA
  GGCAACAAGAGCTTCACTCTGGATGCCCGCCTCGTGGAGTTCCTCTGGGTGCCAGATACT
  TACATTGTGGAGTCCAAGAAGTCCTTCCTCCATGAAGTCACTGTGGGAAACAGGCTCATC
  CGCCTCTTCTCCAATGGCACGGTCCTGTATGCCCTCAGAATCACGACAACTGTTGCATGT
  AACATGGATCTGTCTAAATACCCCATGGACACACAGACATGCAAGTTGCAGCTGGAAAGC
  TGGGGCTATGATGGAAATGATGTGGAGTTCACCTGGCTGAGAGGGAACGACTCTGTGCGT
  GGACTGGAACACCTGCGGCTTGCTCAGTACACCATAGAGCGGTATTTCACCTTAGTCACC
  AGATCGCAGCAGGAGACAGGAAATTACACTAGATTGGTCTTACAGTTTGAGCTTCGGAGG
  AATGTTCTGTATTTCATTTTGGAAACCTACGTTCCTTCCACTTTCCTGGTGGTGTTGTCC
  TGGGTTTCATTTTGGATCTCTCTCGATTCAGTCCCTGCAAGAACCTGCATTGGAGTGACG
  ACCGTGTTATCAATGACCACACTGATGATCGGGTCCCGCACTTCTCTTCCCAACACCAAC
  TGCTTCATCAAGGCCATCGATGTGTACCTGGGGATCTGCTTTAGCTTTGTGTTTGGGGCC
  TTGCTAGAATATGCAGTTGCTCACTACAGTTCCTTACAGCAGATGGCAGCCAAAGATAGG
  GGGACAACAAAGGAAGTAGAAGAAGTCAGTATTACTAATATCATCAACAGCTCCATCTCC
  AGCTTTAAACGGAAGATCAGCTTTGCCAGCATTGAAATTTCCAGCGACAACGTTGACTAC
  AGTGACTTGACAATGAAAACCAGCGACAAGTTAAAGTTTGTCTTCCGAGAAAAGATGGGC
  AGGATTGTTGATTATTTCACAATTCAAAACCCCAGTAATGTTGATCACTATTCCAAACTA
  CTGTTTCCTTTGATTTTTATGCTAGCCAATGTATTTTACTGGGCATACTACATGTATTTT
  TGA
  
  Clone variation with respect to NM_014211.2
  1173 c=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014211 unedited
  CAAATTTTGTATACGACTCACTATAGGGNNCGGCCGCGNNAATTCGGCACCCAGGTCCAC
  ATACGAGGGGACAGGGCTGAGGATGAGGAGAACCCTGGGGACCCAGAAGACCGTGCCTTG
  CCCGGAAGTCCTGCCTGTAGGCCTGAAGGACTTGCCCTAACAGAGCCTCAACAACTACCT
  GGTGATTCCTACTTCAGCCCCTTGGTGTGAGCAGCTTCTCAACATGAACTACAGCCTCCA
  CTTGGCCTTCGTGTGTCTGAGTCTCTTCACTGAGAGGATGTGCATCCAGGGGAGTCAGTT
  CAACGTCGAGGTCGGCAGAAGTGACAAGCTTTCCCTGCCTGGCTTTGAGAACCTCACAGC
  AGGATATAACAAATTTCTCAGGCCCAATTTTGGTGGAGAACCCGTACAGATAGCGCTGAC
  TCTGGACATTGCAAGTATCTCTAGCATTTCAGAGAGTAACATGGACTACACAGCCACCAT
  ATACCTCCGACAGCGCTGGATGGACCAGCGGCTGGTGTTTGAAGGCAACAAGAGCTTCAC
  TCTGGATGCCCGCCTCGTGGAGTTCCTCTGGGTGCCAGATACTTACATTGTGGAGTCCAA
  GAAGTCCTTCCTCCATGAAGTCACTGTGGGAAACAGGCTCATCCGCCTCTTCTCCATGGC
  ACGGTCCTGTATGCCCTCAGAATCACGACAACTGTTGCATGTAAACATGGATCTGTCTAA
  ATACCCCATGGACACACAGACATGCAAGTTGCAGCTGGAAAGCTGGGGCTATGATGGAAA
  TGATGTTGGAGTTCACCTGGCTGAGAGGACGAAACTCTGTGCGTGGACTGGAACACCCTG
  CGCTTGCTCAGTACACCATAGAGCGTATTTCACCTTTATCACCAGATCGCAGCAGGAGAC
  AGGGAATACACTAGATGGNCTTACAGTTGAGCTCGNAAGAAATGTCTGNATTTCATTTGA
  AAACTACGTCTCTCCTTGGGGGGGGGGTGCCCCGGGTTAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014211 unedited
  ACTATGGAACGCGGCACGCAANCTAGTATCGAGTTTTTTTTTTTTTTTTTTTTGGTGGGT
  AGCTAATATGGTATATTTATTTCACAGTTTCACATCTTTATCATTGGTTTTATATAAAAA
  CAATGCTTAAGTGGGGTTTCAGAACAGATATTTCCTTTTAAACTTTTTTTAAAAAATCAC
  AAATATGATTGGCTCATACAACCACATTTCACCTCTTTTCACCAGCACTCCCACCCATTC
  CTGTTAGAAATATTTTGTTAAAAAAATCAGGTGATCAAACTCATAGAAACTGAATTGTGA
  GAAGTATAATGGGAAAAGGAATGAGAACCTGTGGCTCTAGGGAGTTACAGAAGGGAAATC
  ATCTTTTAAAGCCTTGGTTTATTTCTGACAGGAAAGGTAAAGCCGTGCATTTATTAGAAC
  AGGAAGCTAAGAAATTAAAGATGGCGAGATTGTCTAAAATAACTGAGGCTGAACTGGAAG
  CCTTTACCCTGGCACACTCAAAAATCACTTTTGGTACTGTTGGCTGAAACTGGCCGAGGA
  ATGTGCATCTCTGGCTGGATTTGGAGAGGGCAGCTCACCCGCAACCTGAACATAAGTTCT
  GTACCGTATCTCATTGGTACTCATAAAGTTCAGTACTCAAGGCCAGCACGCTCCTGCTCT
  TGCGGGATAATCCACTCACATCAGACCCCCTGGAAGGATCAGGGATCTGAACAAAACCAA
  GTTACTTAAGCTAAACCACTTGTTCCTTGGTCATACCCTCCCGTTCCTATAAGCATATTC
  CTGATCTTACACCAAGGGCTGAAGCCCCCCTTGGAAGATACAGGGATTTGCCCCTGGCAA
  TTGTTGCTCATGTTAATATCCCGCCTCATCACTATGCAGCACCTCTGAGCAACCCAGTTC
  TTTTAATCACCGCCCTTGCGACAAAGCACCTCTAAATGTTCTCCTCCCTCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014211
• Insert Size: 3330 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014211.1, NP_055026.1
• RefSeq Size: 3282 bp
• RefSeq ORF: 1323 bp
• Locus ID: 2568
• UniProt ID: O00591
• Cytogenetics: 5q35.1
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. The subunit encoded by this gene is expressed in several non-neuronal tissues including the uterus and ovaries. This subunit can assemble with known GABA A receptor subunits, and the presence of this subunit alters the sensitivity of recombinant receptors to modulatory agents such as pregnanolone. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
  Transcript Variant: This variant (1) encodes the longer isoform (1).