GABRP (NM_014211) Human Tagged ORF Clone

CAT#: RC224902

GABRP (Myc-DDK-tagged)-Human gamma-aminobutyric acid (GABA) A receptor, pi (GABRP)

Product Images

Circular map for RC224902

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GABRP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224902 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTACAGCCTCCACTTGGCCTTCGTGTGTCTGAGTCTCTTCACTGAGAGGATGTGCATCCAGGGGA
  GTCAGTTCAACGTCGAGGTCGGCAGAAGTGACAAGCTTTCCCTGCCTGGCTTTGAGAACCTCACAGCAGG
  ATATAACAAATTTCTCAGGCCCAATTTTGGTGGAGAACCCGTACAGATAGCGCTGACTCTGGACATTGCA
  AGTATCTCTAGCATTTCAGAGAGTAACATGGACTACACAGCCACCATATACCTCCGACAGCGCTGGATGG
  ACCAGCGGCTGGTGTTTGAAGGCAACAAGAGCTTCACTCTGGATGCCCGCCTCGTGGAGTTCCTCTGGGT
  GCCAGATACTTACATTGTGGAGTCCAAGAAGTCCTTCCTCCATGAAGTCACTGTGGGAAACAGGCTCATC
  CGCCTCTTCTCCAATGGCACGGTCCTGTATGCCCTCAGAATCACGACAACTGTTGCATGTAACATGGATC
  TGTCTAAATACCCCATGGACACACAGACATGCAAGTTGCAGCTGGAAAGCTGGGGCTATGATGGAAATGA
  TGTGGAGTTCACCTGGCTGAGAGGGAACGACTCTGTGCGTGGACTGGAACACCTGCGGCTTGCTCAGTAC
  ACCATAGAGCGGTATTTCACCTTAGTCACCAGATCGCAGCAGGAGACAGGAAATTACACTAGATTGGTCT
  TACAGTTTGAGCTTCGGAGGAATGTTCTGTATTTCATTTTGGAAACCTACGTTCCTTCCACTTTCCTGGT
  GGTGTTGTCCTGGGTTTCATTTTGGATCTCTCTCGATTCAGTCCCTGCAAGAACCTGCATTGGAGTGACG
  ACCGTGTTATCAATGACCACACTGATGATCGGGTCCCGCACTTCTCTTCCCAACACCAACTGCTTCATCA
  AGGCCATCGATGTGTACCTGGGGATCTGCTTTAGCTTTGTGTTTGGGGCCTTGCTAGAATATGCAGTTGC
  TCACTACAGTTCCTTACAGCAGATGGCAGCCAAAGATAGGGGGACAACAAAGGAAGTAGAAGAAGTCAGT
  ATTACTAATATCATCAACAGCTCCATCTCCAGCTTTAAACGGAAGATCAGCTTTGCCAGCATTGAAATTT
  CCAGCGACAACGTTGACTACAGTGACTTGACAATGAAAACCAGCGACAAGTTAAAGTTTGTCTTCCGAGA
  AAAGATGGGCAGGATTGTTGATTATTTCACAATTCAAAACCCCAGTAATGTTGATCACTATTCCAAACTA
  CTGTTTCCTTTGATTTTTATGCTAGCCAATGTATTTTACTGGGCATACTACATGTATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224902 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNYSLHLAFVCLSLFTERMCIQGSQFNVEVGRSDKLSLPGFENLTAGYNKFLRPNFGGEPVQIALTLDIA
  SISSISESNMDYTATIYLRQRWMDQRLVFEGNKSFTLDARLVEFLWVPDTYIVESKKSFLHEVTVGNRLI
  RLFSNGTVLYALRITTTVACNMDLSKYPMDTQTCKLQLESWGYDGNDVEFTWLRGNDSVRGLEHLRLAQY
  TIERYFTLVTRSQQETGNYTRLVLQFELRRNVLYFILETYVPSTFLVVLSWVSFWISLDSVPARTCIGVT
  TVLSMTTLMIGSRTSLPNTNCFIKAIDVYLGICFSFVFGALLEYAVAHYSSLQQMAAKDRGTTKEVEEVS
  ITNIINSSISSFKRKISFASIEISSDNVDYSDLTMKTSDKLKFVFREKMGRIVDYFTIQNPSNVDHYSKL
  LFPLIFMLANVFYWAYYMYF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014211
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014211.3
• RefSeq Size: 3310 bp
• RefSeq ORF: 1323 bp
• Locus ID: 2568
• UniProt ID: O00591
• Cytogenetics: 5q35.1
• Domains: Neur_chan_memb, Neur_chan_LBD
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 50.6 kDa
• Gene Summary: The gamma-aminobutyric acid (GABA) A receptor is a multisubunit chloride channel that mediates the fastest inhibitory synaptic transmission in the central nervous system. The subunit encoded by this gene is expressed in several non-neuronal tissues including the uterus and ovaries. This subunit can assemble with known GABA A receptor subunits, and the presence of this subunit alters the sensitivity of recombinant receptors to modulatory agents such as pregnanolone. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]