CD299 (CLEC4M) (NM_014257) Human Untagged Clone

CAT#: SC115079

CLEC4M (untagged)-Human C-type lectin domain family 4, member M (CLEC4M), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD299
• Synonyms: CD209L; CD299; DC-SIGN2; DC-SIGNR; DCSIGNR; HP10347; L-SIGN; LSIGN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014257 edited
  TGAGGATCAGAGGGTGGAAAATAAAAGCTGTGGTCCCCAGGAGTCCTGAACATCTGGGGA
  CAGCGGGAAAACATGAGTGACTCCAAGGAACCAAGGGTGCAGCAGCTGGGCCTCCTGGAA
  GAAGATCCAACAACCAGTGGCATCAGACTTTTTCCAAGAGACTTTCAATTCCAGCAGATA
  CATGGCCACAAGAGCTCTACAGGGTGTCTTGGCCATGGCGCCCTGGTGCTGCAACTCCTC
  TCCTTCATGCTCTTGGCTGGGGTCCTGGTGGCCATCCTTGTCCAAGTGTCCAAGGTCCCC
  AGCTCCCTAAGTCAGGAACAATCCGAGCAAGACGCAATCTACCAGAACCTGACCCAGCTT
  AAAGCTGCAGTGGGTGAGCTCTCAGAGAAATCCAAGCTGCAGGAGATCTACCAGGAGCTG
  ACCCAGCTGAAGGCTGCAGTGGGTGAGTTGCCAGAGAAATCCAAGCTGCAGGAGATCTAC
  CAGGAGCTGACCCGGCTGAAGGCTGCAGTGGGTGAGTTGCCAGAGAAATCCAAGCTGCAG
  GAGATCTACCAGGAGCTGACCCGGCTGAAGGCTGCAGTGGGTGAGTTGCCAGAGAAATCC
  AAGCTGCAGGAGATCTACCAGGAGCTGACCCGGCTGAAGGCTGCAGTGGGTGAGTTGCCA
  GAGAAATCCAAGCTGCAGGAGATCTACCAGGAGCTGACGGAGCTGAAGGCTGCAGTGGGT
  GAGTTGCCAGAGAAATCCAAGCTGCAGGAGATCTACCAGGAGCTGACCCAGCTGAAGGCT
  GCAGTGGGTGAGTTGCCAGACCAGTCCAAGCAGCAGCAAATCTATCAAGAACTGACCGAT
  TTGAAGACTGCATTTGAACGCCTGTGCCGCCACTGTCCCAAGGACTGGACATTCTTCCAA
  GGAAACTGTTACTTCATGTCTAACTCCCAGCGGAACTGGCACGACTCCGTCACCGCCTGC
  CAGGAAGTGAGGGCCCAGCTCGTCGTAATCAAAACTGCTGAGGAGCAGAACTTCCTACAG
  CTGCAGACTTCCAGGAGTAACCGCTTCTCCTGGATGGGACTTTCAGACCTAAATCAGGAA
  GGCACGTGGCAATGGGTGGACGGCTCACCTCTGTCACCCAGCTTCCAGCGGTACTGGAAC
  AGTGGAGAACCCAACAATAGCGGGAATGAAGACTGTGCGGAATTTAGTGGCAGTGGCTGG
  AACGACAATCGATGTGACGTTGACAATTACTGGATCTGCAAAAAGCCCGCAGCCTGCTTC
  AGAGACGAATAGTTGTTTCCCTGCTAGCCTCAGCCTCCATTGTGGTATAGCAGAACTTCA
  CCCACTTGTAAGCCAGCGCTTCTTCTCTCCATCCTTGGACCTTCACAAATGCCCTGAGAC
  GGTTCTCTGTTCGATTTTTCATCCCCTATGAACCTGGGTCTTATTCTGTCCTTCTGATGC
  CTCCAAGTTTCCCTGGTGTAGAGCTTGTGTTCTTGGCCCATCCTTGGAGCTTTATAAGTG
  ACCTGAGTGGGATGCATTTAGGGGGCGGGCTTGGTATGTTGTATGAATCCACTCTCTGTT
  CCTTTTGGAGATTAGACTATTTGGATTCATGTGTAGCTGCCCTGTCCCCTGGGGCTTTAT
  CTCATCCATGCAAACTACCATCTGCTCAACTTCCAGCTACACCCCGTGCACCCTTTTGAC
  TGGGGACTTGCTGGTTGAAGGAGCTCATCTTGCAGGCTGGAAGCACCAGGGAATTAATTC
  CCCCAGTCAACCAATGGCATCCAGAGAGGGCATGGAGGCTCCATACAACCTCTTCCACCC
  CCACATCTTTCTTTGTCCTATACATGTCTTCCATTTGGCTGTTTCTGAGTTGTAGCCTTT
  ATAATAAAGTGGTAAATGTTGTAACTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014257 unedited
  NGGACGTTCACATTTGTAAACGACTCATATAGGGCGGCCGCGATTCCCGGGATTGAGGAT
  CAGAGGGTGNGAAAATAAAAGCTGTGGTCCCCAGGAGTCCTGACATCTGGGGACAGCGGG
  AAAACATGAGTGACTCCAAGGAACCAAGGGTGCAGCAGCTGGGCCTCCTGGAAGAAGATC
  CAACAACCAGTGGCATCAGACTTTTTCCAAGAGACTTTCAATTCCAGCAGATACATGGCC
  ACAAGAGCTCTACAGGGTGTCTTGGCCATGGCGCCCTGGTGCTGCAACTCCTCTCCTTCA
  TGCTCTTGGCTGGGGTCCTGGTGGCCATCCTTGTCCAAGTGTCCAAGGTCCCCAGCTCCC
  TAAGTCAGGAACAATCCGAGCAAGACGCAATCTACCAGAACCTGACCCAGCTTAAAGCTG
  CAGTGGGTGAGCTCTCAGAGAAATCCAAGCTGCAGGAGATCTACCAGGAGCTGACCCAGC
  TGAAGGCTGCAGTGGGTGAGTTGCCAGAGAAATCCAAGCTGCAGGAGATCTACCAGGAGC
  TGACCCGGCTGAAGGCTGCAGTGGGTGAGTTGCCAGAGAAATCCAAGCTGCAGGAGATCT
  ACCAGGAGCTGACCCGGCTGAAGGCTGCAGTGGGTGAGTTGCCAGAGAAATCCAAGCTGC
  AGGAGATCTACCAGGAGCTGACCCGGCTGAAGGCTGCAGTGGGTGAGTTGCCAGAGAAAT
  CCAAGCTGCAGGAGATCTACCAGGAGCTGACGGAGCTGAAGGCTGCAGTGGGTGAGTTGC
  CAGAGAAATCCAAGCTGCANGAGATCTACCAGGAGCTGACCCAGCTGAANGCTGCAGTGG
  GTGAGTTGCCAGACCAGTCCAAGCAGCAGCAAATCTATCAAGAACTGACCGATTGNGAGA
  CTGCA
  
• Restriction Sites: Please inquire
• ACCN: NM_014257
• Insert Size: 1930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014257.3, NP_055072.3
• RefSeq Size: 1930 bp
• RefSeq ORF: 1200 bp
• Locus ID: 10332
• UniProt ID: Q9H2X3
• Cytogenetics: 19p13.2
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a C-type lectin that functions in cell adhesion and pathogen recognition. This receptor recognizes a wide range of evolutionarily divergent pathogens with a large impact on public health, including tuberculosis mycobacteria, and viruses including Ebola, hepatitis C, HIV-1, influenza A, West Nile virus and the SARS-CoV acute respiratory syndrome coronavirus. The protein is organized into four distinct domains: a C-terminal carbohydrate recognition domain, a flexible tandem-repeat neck domain of variable length, a transmembrane region and an N-terminal cytoplasmic domain involved in internalization. This gene is closely related in terms of both sequence and function to a neighboring gene, CD209 (Gene ID: 30835), also known as DC-SIGN. The two genes differ in viral recognition and expression patterns, with this gene showing high expression in endothelial cells of the liver, lymph node and placenta. Polymorphisms in the tandem repeat neck domain are associated with resistance to SARS infection. [provided by RefSeq, May 2020]
  Transcript Variant: This variant (1) encodes the longest isoform (1).