HERPUD1 (NM_014685) Human Untagged Clone

CAT#: SC114911

HERPUD1 (untagged)-Human homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1 (HERPUD1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HERPUD1
• Synonyms: HERP; Mif1; SUP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114911 sequence for NM_014685 edited (data generated by NextGen Sequencing)
  ATGGAGTCCGAGACCGAACCCGAGCCCGTCACGCTCCTGGTGAAGAGCCCCAACCAGCGC
  CACCGCGACTTGGAGCTGAGTGGCGACCGCGGCTGGAGTGTGGGCCACCTCAAGGCCCAC
  CTGAGCCGCGTCTACCCCGAGCGTCCGCGTCCAGAGGACCAGAGGTTAATTTATTCTGGG
  AAGCTGTTGTTGGATCACCAATGTCTCAGGGACTTGCTTCCAAAGCAGGAAAAACGGCAT
  GTTTTGCATCTGGTGTGCAATGTGAAGAGTCCTTCAAAAATGCCAGAAATCAACGCCAAG
  GTGGCTGAATCCACAGAGGAGCCTGCTGGTTCTAATCGGGGACAGTATCCTGAGGATTCC
  TCAAGTGATGGTTTAAGGCAAAGGGAAGTTCTTCGGAACCTTTCTTCCCCTGGATGGGAA
  AACATCTCAAGGCCTGAAGCTGCCCAGCAGGCATTCCAAGGCCTGGGTCCTGGTTTCTCC
  GGTTACACACCCTATGGGTGGCTTCAGCTTTCCTGGTTCCAGCAGATATATGCACGACAG
  TACTACATGCAATATTTAGCAGCCACTGCTGCATCAGGGGCTTTTGTTCCACCACCAAGT
  GCACAAGAGATACCTGTGGTCTCTGCACCTGCTCCAGCCCCTATTCACAACCAGTTTCCA
  GCTGAAAACCAGCCTGCCAATCAGAATGCTGCTCCTCAAGTGGTTGTTAATCCTGGAGCC
  AATCAAAATTTGCGGATGAATGCACAAGGTGGCCCTATTGTGGAAGAAGATGATGAAATA
  AATCGAGATTGGTTGGATTGGACCTATTCAGCAGCTACATTTTCTGTTTTTCTCAGTATC
  CTCTACTTCTACTCCTCCCTGAGCAGATTCCTCATGGTCATGGGGGCCACCGTTGTTATG
  TACCTGCATCACGTTGGGTGGTTTCCATTTAGACCGAGGCCGGTTCAGAACTTCCCAAAT
  GATGGTCCTCCTCCTGACGTTGTAAATCAGGACCCCAACAATAACTTACAGGAAGGCACT
  GATCCTGAAACTGAAGACCCCAACCACCTCCCTCCAGACAGGGATGTACTAGATGGCGAG
  CAGACCAGCCCCTCCTTTATGAGCACAGCATGGCTTGTCTTCAAGACTTTCTTTGCCTCT
  CTTCTTCCAGAAGGCCCCCCAGCCATCGCAAACTGA
  
  Clone variation with respect to NM_014685.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014685 unedited
  TGTAATACGACTCACTATAGGGNNCGGCCGCGNAATTCGGCACGAGGGTCGTTGCAGAGA
  TTGCGGGCGGCTGAGACGCCGCCTGCCTGGCACCTAGGAGCGCAGCGGAGCCCCGACACC
  GCCGCCGCCGCCATGGAGTCCGAGACCGAACCCGAGCCCGTCACGCTCCTGGTGAAGAGC
  CCCAACCAGCGCCACCGCGACTTGGAGCTGAGTGGCGACCGCGGCTGGAGTGTGGGCCAC
  CTCAAGGCCCACCTGAGCCGCGTCTACCCCGAGCGTCCGCGTCCAGAGGACCAGAGGTTA
  ATTTATTCTGGGAAGCTGTTGTTGGATCACCAATGTCTCAGGGACTTGCTTCCAAAGCAG
  GAAAAACGGCATGTTTTGCATCTGGTGTGCAATGTGAAGAGTCCTTCAAAAATGCCAGAA
  ATCAACGCCAAGGTGGCTGAATCCACAGAGGAGCCTGCTGGTTCTAATCGGNGACAGTAT
  CCTGAGGATTCCTCAAGTGATGGTTTAAGGCAAAGGGAAGTTCTTCGGAACCTTTCTTCC
  CCTGGATGGGAAAACATCTCAAGGCCTGAAGCTGCCCAGCAGGCATTCCAAGCCCTGGGG
  TCCTGGTTTCTCCGGTTACACACCCTATGGGGTGGCTTCAGCTTTCCTGGTTCCAGCAGA
  TATATGCACGACAGTACTACATGCAATATTTANCAGCCACTGCTGCATCTAGGGGCCTTT
  TGTTCCACCACCAAGTGCCACAGAGAACCTTGTGGTCTCTGAACCTGCTCCAACCCCTAT
  TCACACCCAGGTTNCCAGCTGAAAACCAGCCTGCCATTAGGATGCTGCTCTCTAAGGGGT
  TGGTAACCTGGAGCCCAATAAATTTTGCCGTGAATGCCCAAGGGGGCCTTTGTGGAAACA
  AC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014685 unedited
  CCGCATCTAGATCGAGTTTTTTTTTTTTTTTTTTAAGGGTCATTCATAGTAAAACTTTAT
  TGAACAGAAAACCCAGCAAAGGTTTTCACCTCCGCAAAGTTCCCCTTAGTTTAAAGTAAA
  GCACTGCATTTTAAAAAGCAATTATACATAAGTCTTTCCTAAAAAAGTCCTGCTAAAACA
  TGTCTAGCAATTTCATTGATTATATAAAGTAGTACACTTAGTGTAATTTAAACATTCCAA
  CAGGAATCAAATCGTACCAGCAGAACCACTTCTGCATCTATGACTTCTATGTACAAACAC
  ACATGCAGACACACACATTTGGAAAAGTTCCTCAAGCATAGACATGCAACACCTAAGGCC
  TTCTACGTACAGGGCTTATTAAACTACATAGAGTATATATTAAAGCTCTTCAGAATAAAG
  ACATGAGAAGCCTTGGGCATTTTTTGTTCACCAATTTGTATCACGGCTTCACGTTTCTGC
  TTTTGCTTGCTCACAAAAGCATATCATCATCCACACTGTTTTTTAAAAACTCATCATTGC
  CATGTCCAGGAGAGGCAATCTAGCTGGAGTCAGGTGATCCAGTCCATTCCTGTCAAAGCC
  TCCAACAGCTACAGCACAAACACCATCAGTTTGCGATGGCTGGGGGGCCTTCTGGAAGAA
  GGAGGCAAAGAAAGCCTTGAAGACAAGCCATGCTGTGCTCATAAAGGAGGGGCTGGTCTG
  CTCGCCATCTANTACATCCCTGTCTGGAGGGAGGTGGTTGGGGTTCTTCAGTTTCAAGAT
  CAGGGCCCTCCTGTAAGTTATTGTTGGGGTCCTGATTTAAACCGTCAAGAGGATGACCTC
  ATCTTGGGAGTTCTGAACCGCGCTCGGTCTAAATGGAATCCACCCACGTGATGCAGTACA
  TACAACGGGGGCCCCATGACACTGAGATCTGCCAGGGAGGAGTAATTCCAGATCCTGGAA
  ACAAAAATGACTGCGGAAAGTCCATCACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014685
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014685.2, NP_055500.1
• RefSeq Size: 2198 bp
• RefSeq ORF: 1176 bp
• Locus ID: 9709
• UniProt ID: Q15011
• Cytogenetics: 16q13
• Domains: UBQ
• Protein Families: Druggable Genome
• Gene Summary: The accumulation of unfolded proteins in the endoplasmic reticulum (ER) triggers the ER stress response. This response includes the inhibition of translation to prevent further accumulation of unfolded proteins, the increased expression of proteins involved in polypeptide folding, known as the unfolded protein response (UPR), and the destruction of misfolded proteins by the ER-associated protein degradation (ERAD) system. This gene may play a role in both UPR and ERAD. Its expression is induced by UPR and it has an ER stress response element in its promoter region while the encoded protein has an N-terminal ubiquitin-like domain which may interact with the ERAD system. This protein has been shown to interact with presenilin proteins and to increase the level of amyloid-beta protein following its overexpression. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. The full-length nature of all transcript variants has not been determined. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).