AASDHPPT (NM_015423) Human Untagged Clone

CAT#: SC114644

AASDHPPT (untagged)-Human aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase (AASDHPPT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AASDHPPT
• Synonyms: AASD-PPT; ACPS; CGI-80; LYS2; LYS5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114644 sequence for NM_015423 edited (data generated by NextGen Sequencing)
  ATGGTTTTCCCTGCCAAACGGTTCTGCTTGGTGCCATCCATGGAGGGCGTGCGCTGGGCC
  TTTTCCTGCGGCACTTGGCTGCCGAGCCGAGCCGAATGGCTGCTGGCAGTGCGATCGATT
  CAGCCCGAGGAGAAGGAGCGCATTGGCCAGTTCGTCTTTGCCCGGGACGCTAAGGCAGCC
  ATGGCTGGTCGTCTGATGATAAGGAAATTAGTTGCAGAGAAATTGAATATCCCTTGGAAT
  CATATTCGTTTGCAAAGAACTGCAAAAGGAAAACCAGTTCTTGCAAAGGACTCATCGAAT
  CCTTACCCGAATTTCAACTTTAACATCTCTCATCAAGGAGACTATGCAGTGCTTGCTGCT
  GAACCTGAGCTGCAAGTTGGAATTGATATAATGAAGACTAGTTTTCCAGGTCGTGGTTCA
  ATTCCAGAATTCTTTCATATTATGAAAAGAAAGTTTACCAACAAAGAATGGGAAACAATC
  AGAAGCTTTAAGGATGAGTGGACTCAGCTGGATATGTTTTATAGGAATTGGGCACTTAAG
  GAAAGCTTCATAAAAGCCATTGGTGTTGGACTAGGATTTGAATTGCAGCGGCTTGAATTT
  GATCTATCTCCATTAAACTTGGATATAGGCCAAGTTTATAAAGAAACACGTTTATTCCTG
  GATGGAGAGGAAGAAAAAGAATGGGCATTTGAGGAAAGCAAAATAGATGAGCACCATTTT
  GTTGCAGTTGCTCTTAGGAAACCCGATGGATCTAGACATCAGGATGTTCCATCTCAGGAT
  GATTCCAAACCAACCCAGAGGCAATTTACTATTCTCAACTTTAATGATTTAATGTCATCT
  GCCGTTCCCATGACACCTGAAGATCCTTCATTTTGGGACTGTTTTTGCTTCACAGAAGAA
  ATTCCAATACGAAATGGTACAAAGTCATGA
  
  Clone variation with respect to NM_015423.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015423 unedited
  ATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAAT
  TCGGCACGAGGAGATAGCGGCGAGGTCCGCTTTCAGTGTATGGTTTTCCCTGCCAAACGG
  TTCTGCTTGGTGCCATCCATGGAGGGCGTGCGCTGGGCCTTTTCCTGCGGCACTTGGCTG
  CCGAGCCGAGCCGAATGGCTGCTGGCAGTGCGATCGATTCAGCCCGAGGAGAAGGAGCGC
  ATTGGCCAGTTCGTCTTTGCCCGGGACGCTAAGGCAGCCATGGCTGGCCGTCTGATGATA
  AGGAAATTAGTTGCAGAGAAATTGAATATCCCTTGGAATCATATTCGTTTGCAAAGAACT
  GCAAAAGGAAAACCAGTTCTTGCAAAGGACTCATCGAATCCTTACCCGAATTTCAACTTT
  AACCATCTCTCATCAAGGAGACTATGCAGTGCTTGCTGCTGAACCTGAGCTGCAAGTTGG
  AATTGATATAATGAAGACTAGTTTTCCAGGTCGTGGTTCAATTCCAGAATTCTTTCATAT
  TATGAAAAGAAAGTTTACCAACAAAGAATGGGAAACAATCAGAAGCTTTAAGGATGAGTG
  GACTCAGCTGGATATGTTTTATAGGAATTGGGCACTTAACGAAAGCTCCATAAAAGCCAT
  TGGTGTTGGACTAGGATTTGAATTGCAGCGGCTTGAATTTGATCTATCTCCATTAAACTT
  GGATATAGGCCAAGTTTATAAAGACACACGTCTATTCCCTGGATGCAGACGCAGAAATAG
  AATGGGCATTTGAGGAAAGCAAATAGATGAGCACCATTTTGTTGCAGTTGCTCTTAAGAA
  ACCCGATGCATCTACACCTCAAGATGTCCCTCTTAGGATGATTCCAACCACACCAGAGGC
  ATTTATATTCTCACCTTTATGATTTATGCCATCGCCCGTCCATGACACCGAAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015423 unedited
  ACCTATGCACCGCGGCNCGCTTTTCTACGATCGAGTTTTTTTTTTTTTTTTTTCCATAAT
  AAATGTTCATTTTTTTATTATTATAATGACAAGACAAAGTTTTTCATATCACCATTACAC
  TCATGCTAGGGATAACAATACTTCCTCTATTATAAATGCTACTTGACCTTTATTTACTAT
  GCTTGGCATAACTGGGCATGCTTAATAAAATAAAAAAAAAAAACATTCAAATTTTTTTCT
  TTTACTAATCGAAGTACCTAATTTTATACAATTTGCACACTTTTAATGTTAGTAACAGAA
  ACAATGAAATTATTTTACTTCTTGATACTTAAGAGTTTAACTCCAAGACATGCACTACAA
  CCACATAATAGATTTTTGTCATCAATATGATGGTCCTGGCATCTTATATATTAAGTTCAC
  ACTTTAAATTAAGTACTACTTGCTAAAACGAAAACAGGTAAAAACAATGAACAGACGGTT
  CACGTTAAGAGGAATACGAAAAGCCAATAACAATACTGACATAACAATCTTATTTGTACT
  GAACGAAATACAAATTTTAAAAATGACATACCATTTTTTAATTACATTGGCAAAAATTAA
  ACCAAAAAGAATACCTACAATTGACCAAGGAGTATTCTGGTAGGGGGAGGGACGCTACAT
  CCATATAGCACTAATTAAAGGGGCCAATTGTATAATTAAAGCCCTATGAACCAGTCAAAC
  CCATCATTTTACTTTTACCAACTTTCTCAGGACATAATTAAGCATGTAACTTCAAAAGTA
  CAGAGAAATTACTCTGCCACTGCTTGCACAACCAAATGCAAATATCCAAATGTCTACCAC
  AGAAAAAGGGAGCACACATTGTAAACCTTCTTACGGGAGGCCAGGGGCCTTCCAATTCCA
  TTTTCAACCCTACTAACCTTTTTCCTTTAAACAAACTCCCTTCAACCCCAACACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015423
• Insert Size: 2870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015423.2, NP_056238.2
• RefSeq Size: 2880 bp
• RefSeq ORF: 930 bp
• Locus ID: 60496
• UniProt ID: Q9NRN7
• Cytogenetics: 11q22.3
• Domains: ACPS
• Protein Pathways: Lysine biosynthesis, Lysine degradation, Metabolic pathways
• Gene Summary: The protein encoded by this gene is similar to Saccharomyces cerevisiae LYS5, which is required for the activation of the alpha-aminoadipate dehydrogenase in the biosynthetic pathway of lysine. Yeast alpha-aminoadipate dehydrogenase converts alpha-biosynthetic-aminoadipate semialdehyde to alpha-aminoadipate. It has been suggested that defects in the human gene result in pipecolic acidemia. [provided by RefSeq, Jul 2008]