Pokemon (ZBTB7A) (NM_015898) Human Untagged Clone

CAT#: SC114581

ZBTB7A (untagged)-Human zinc finger and BTB domain containing 7A (ZBTB7A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Pokemon
• Synonyms: FBI-1; FBI1; LRF; pokemon; TIP21; ZBTB7; ZNF857A
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015898, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCGGCGGCGTGGACGGCCCCATCGGGATCCCGTTCCCCGACCACAGCAGCGACATCCTGAGTGGGC
  TGAACGAGCAGCGGACGCAGGGCCTGCTGTGCGACGTGGTGATCCTGGTGGAGGGCCGCGAGTTCCCCAC
  GCACCGCTCGGTGCTGGCCGCCTGCAGCCAGTACTTCAAGAAGCTGTTCACGTCGGGCGCCGTGGTGGAC
  CAGCAGAACGTGTACGAGATCGACTTCGTCAGCGCCGAGGCGCTCACCGCGCTCATGGACTTCGCCTACA
  CGGCCACGCTCACCGTCAGCACAGCCAACGTGGGTGACATCCTCAGCGCCGCCCGCCTGCTGGAGATCCC
  CGCCGTGAGCCACGTGTGCGCCGACCTCCTGGACCGGCAGATCCTGGCGGCCGACGCGGGCGCCGACGCC
  GGGCAGCTGGACCTTGTAGATCAAATTGATCAGCGCAACCTCCTCCGCGCCAAGGAGTACCTCGAGTTCT
  TCCAGAGCAACCCCATGAACAGCCTGCCCCCCGCGGCCGCCGCCGCCGCTGCCAGCTTCCCGTGGTCCGC
  CTTTGGGGCGTCCGATGATGACCTGGATGCCACCAAGGAGGCCGTGGCCGCCGCTGTGGCCGCCGTGGCC
  GCGGGCGACTGCAACGGCTTAGACTTCTATGGGCCGGGCCCCCCGGCCGAGCGGCCCCCGACGGGGGACG
  GGGACGAGGGCGACAGCAACCCGGGTCTGTGGCCAGAGCGGGATGAGGACGCCCCCACCGGGGGTCTCTT
  TCCGCCGCCGGTGGCCCCGCCGGCCGCCACGCAGAACGGCCACTACGGCCGCGGCGGAGAGGAGGAGGCC
  GCCTCGCTGTCGGAGGCGGCCCCCGAGCCGGGCGACTCTCCGGGCTTCCTGTCGGGAGCGGCCGAGGGCG
  AGGACGGGGACGGGCCCGACGTGGACGGGCTGGCGGCCAGCACGCTGCTGCAGCAGATGATGTCATCGGT
  GGGCCGGGCGGGGGCCGCGGCGGGGGACAGCGACGAGGAGTCGCGGGCCGACGACAAGGGCGTCATGGAC
  TACTACCTGAAGTACTTCAGCGGCGCCCACGACGGCGACGTCTACCCGGCCTGGTCGCAGAAGGTGGAGA
  AGAAGATCCGAGCCAAGGCCTTCCAGAAGTGCCCCATCTGCGAGAAGGTCATCCAGGGCGCCGGCAAGCT
  GCCGCGACACATCCGCACCCACACGGGCGAGAAGCCCTACGAGTGCAACATCTGCAAGGTCCGCTTCACC
  AGGCAGGACAAGCTGAAGGTGCACATGCGGAAGCACACGGGCGAGAAGCCGTACCTGTGCCAGCAGTGCG
  GCGCCGCCTTTGCCCACAACTACGACCTGAAGAACCACATGCGCGTGCACACGGGCCTGCGCCCCTACCA
  GTGCGACAGCTGCTGCAAGACCTTCGTCCGCTCCGACCACCTGCACAGACACCTCAAGAAAGACGGCTGC
  AACGGCGTCCCCTCGCGCCGCGGCCGCAAGCCCCGCGTCCGGGGCGGGGCGCCCGACCCCAGCCCGGGGG
  CCACCGCGACCCCCGGCGCCCCCGCCCAGCCCAGCTCCCCCGACGCCCGGCGCAACGGCCAGGAGAAGCA
  CTTTAAGGACGAGGACGAGGACGAGGACGTGGCCAGCCCCGACGGCTTGGGCCGGTTGAATGTAGCGGGC
  GCCGGTGGAGGAGGTGACAGCGGAGGTGGCCCCGGGGCCGCCACCGACGGTAACTTCACAGCCGGACTCG
  CCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015898 unedited
  CCCCGCCCCGTTGGCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGA
  GCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAAT
  TCGGCACGAGGGCGACGCGGGCAGAGCGAGCGCCGCGGACCCCGGAGGCGAGCGGCCGAG
  GGAGCCCAGTCCCAACTTCGGGCCCCGGCCCCGCGCGCCCCGGCCCGGCCCCGCGAGGTC
  TCGGCGCGGAAGATGGCCGGCGGCGTGGACGGCCCCATCGGGATCCCGTTCCCCGACCAC
  AGCAGCGACATCCTGAGTGGGCTGAACGAGCAGCGGACGCAGGGCCTGCTGTGCGACGTG
  GTGATCCTGGTGGAGGGCCGCGAGTTCCCCACGCACCGCTCGGTGCTGGCCGCCTGCAGC
  CAGTACTTCAAGAAGCTGTTCACGTCGGGCGCCGTGGTGGACCAGCAGAACGTGTACGAG
  ATCGACTTCGTCAGCGCCGAGGCGCTCACCGCGCTCATGGACTTCGCCTACACGGCCACG
  CTCACCGTCAGCACAGCCAACGTGGGTGACATCCTCAGCGCCGCCCGCCTGCTGGAGATC
  CCCGCCGTGAGCCACGTGTGCGCCGACCTCCTGGACCGGCAGATCCTGGCGGCCGACGCG
  GGCGCCGACGCCGGGCAGCTGGACCTTGTAGATCAAATTGATCAGCGCAACCTCCTCCGC
  GCCAAGGAGTACCTCGAGTTCTTCCAGAGCAACCCCATGAACAGCCTGCCCCCCGCGGNC
  GNCGNCGCCGCTGCCAGCTTCCCGTGGGTCGNCTTTGGNGCGTTCGATGATGACCTGGAT
  GCACCANGNNAGCGTGGCCGNCGCTGTGGCCNCGTGGGCGGGNCGACTGCACGGCTANAC
  TCTATGGGGCGGGCCNNCGGCGAGCGGCCCGAGGGGGACGGGGACAAGCGACGNACCCGG
  TCTGGGCGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015898 unedited
  TNANGATCGAGTTTTTTTTTTTTTTTTTTGTGTTTTTGGGGGGGTGGTGGGTGATTTTTT
  TTCTCTCTCTCTGTCTCTCTCTTTCTCGGGTTTCTGTTTTCTCTTTTTGGTTTTTAGGCG
  AGTCCGGCTGTGAAGTTACCGTCGGTGGCGGCCCCGGGGCCACCTCCGCTGTCACCTCCT
  CCACCGGCGCCCGCTACATTCAACCGGCCCAAGCCGTGGGGGCTGGCCACGTCCTCGTCC
  TCGTCCTCGTCCTTAAAGTGCTTCTCCTGGCCGTTGCGCCGGGCGTCGGGGGAGCTGGGC
  TGGGCCGGGGCGCCGGGGGTCGCGGTGGCCCCCGGGCTGGGGTCGGACGCCCCGCCCCGG
  ACGCGGGGCTTGCGGCCCGGGCGCCAGGGGGCCCCGTTGCAGCCGCCTTTCTTGCGGGGC
  CTGGGCAAGTGGGCCGGACCGAACGAAAGTATTGCATCAGCTACGCCCCTGTAGGGGCCC
  CCACCCCATTTCACGAGCTGTCCCTGTTGAGGCGCGGAGGTGCGGGCACTAGGAGTGCCG
  GCCGGCTCCTCACTTCTTCTTCAGCCCCCCGCGCCGCCGGCAGGGCCCCCTGGGGCACGT
  CGCAGGGGGGCGCGACGCCTTTTGTTTTGCACCCCCCGCACCGGGTGTTGCGCCCGTGGC
  GCGCGGGTTGTTTGGGTGAATTGTGGTGATGCCAGCGACCCGCCCCGCCCGACATCGTTG
  CGCCTTGGGAGTCTTTTCGCCGGCCCCTTTGTGTTGTAGTGCAGTTCCGGGGCGCGGTTG
  GATCCATCACCTACTCTACACACCCGGCATCACTATCACCCCCAACATCAGCACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015898
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015898.2, NP_056982.1
• RefSeq Size: 4456 bp
• RefSeq ORF: 1755 bp
• Locus ID: 51341
• UniProt ID: O95365
• Cytogenetics: 19p13.3
• Domains: BTB, zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: Transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation (PubMed:14701838, PubMed:17595526, PubMed:20812024, PubMed:25514493, PubMed:26455326, PubMed:26816381). Directly and specifically binds to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3' and represses transcription both by regulating the organization of chromatin and through the direct recruitment of transcription factors to gene regulatory regions (PubMed:12004059, PubMed:17595526, PubMed:20812024, PubMed:25514493, PubMed:26816381). Negatively regulates SMAD4 transcriptional activity in the TGF-beta signaling pathway through these two mechanisms (PubMed:25514493). That is, recruits the chromatin regulator HDAC1 to the SMAD4-DNA complex and in parallel prevents the recruitment of the transcriptional activators CREBBP and EP300 (PubMed:25514493). Collaborates with transcription factors like RELA to modify the accessibility of gene transcription regulatory regions to secondary transcription factors (By similarity). Also directly interacts with transcription factors like SP1 to prevent their binding to DNA (PubMed:12004059). Functions as an androgen receptor/AR transcriptional corepressor by recruiting NCOR1 and NCOR2 to the androgen response elements/ARE on target genes (PubMed:20812024). Thereby, negatively regulates androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Involved in the switch between fetal and adult globin expression during erythroid cells maturation (PubMed:26816381). Through its interaction with the NuRD complex regulates chromatin at the fetal globin genes to repress their transcription (PubMed:26816381). Specifically represses the transcription of the tumor suppressor ARF isoform from the CDKN2A gene (By similarity). Efficiently abrogates E2F1-dependent CDKN2A transactivation (By similarity). Regulates chondrogenesis through the transcriptional repression of specific genes via a mechanism that also requires histone deacetylation (By similarity). Regulates cell proliferation through the transcriptional regulation of genes involved in glycolysis (PubMed:26455326). Involved in adipogenesis through the regulation of genes involved in adipocyte differentiation (PubMed:14701838). Plays a key role in the differentiation of lymphoid progenitors into B and T lineages (By similarity). Promotes differentiation towards the B lineage by inhibiting the T-cell instructive Notch signaling pathway through the specific transcriptional repression of Notch downstream target genes (By similarity). Also regulates osteoclast differentiation (By similarity). May also play a role, independently of its transcriptional activity, in double-strand break repair via classical non-homologous end joining/cNHEJ (By similarity). Recruited to double-strand break sites on damage DNA, interacts with the DNA-dependent protein kinase complex and directly regulates its stability and activity in DNA repair (By similarity). May also modulate the splicing activity of KHDRBS1 toward BCL2L1 in a mechanism which is histone deacetylase-dependent and thereby negatively regulates the pro-apoptotic effect of KHDRBS1 (PubMed:24514149).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.