E3 SUMO protein ligase PIAS4 (PIAS4) (NM_015897) Human Untagged Clone

CAT#: SC114580

PIAS4 (untagged)-Human protein inhibitor of activated STAT, 4 (PIAS4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: E3 SUMO protein ligase PIAS4
• Synonyms: PIAS-gamma; Piasg; PIASY; ZMIZ6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015897, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGGAGCTGGTGGAGGCCAAAAACATGGTGATGAGTTTTCGAGTCTCCGACCTTCAGATGCTCC
  TGGGTTTCGTGGGCCGGAGTAAGAGTGGACTGAAGCACGAGCTCGTCACCAGGGCCCTCCAGCTGGTGCA
  GTTTGACTGTAGCCCTGAGCTGTTCAAGAAGATCAAGGAGCTGTACGAGACCCGCTACGCCAAGAAGAAC
  TCGGAGCCTGCCCCACAGCCGCACCGGCCCCTGGACCCCCTGACCATGCACTCCACCTACGACCGGGCCG
  GCGCTGTGCCCAGGACTCCGCTGGCAGGCCCCAATATTGACTACCCCGTGCTCTACGGAAAGTACTTAAA
  CGGACTGGGACGGTTGCCCGCCAAGACCCTCAAGCCAGAAGTCCGCCTGGTGAAGCTGCCGTTCTTTAAT
  ATGCTGGATGAGCTGCTGAAGCCCACCGAATTAGTCCCACAGAACAACGAGAAGCTTCAGGAGAGCCCGT
  GCATCTTCGCATTGACGCCAAGACAGGTGGAGTTGATCCGGAACTCCAGGGAACTGCAGCCCGGAGTTAA
  AGCCGTGCAGGTCGTCCTGAGAATCTGTTACTCAGACACCAGCTGCCCTCAGGAGGACCAGTACCCGCCC
  AACATCGCTGTGAAGGTCAACCACAGCTACTGCTCCGTCCCGGGCTACTACCCCTCCAATAAGCCCGGGG
  TGGAGCCCAAGAGGCCGTGCCGCCCCATCAACCTCACTCACCTCATGTACCTGTCCTCGGCCACCAACCG
  CATCACTGTCACCTGGGGGAACTACGGCAAGAGCTACTCGGTGGCCCTGTACCTGGTGCGGCAGCTGACC
  TCATCGGAGCTGCTGCAGAGGCTGAAGACCATTGGGGTAAAGCACCCGGAGCTGTGCAAGGCACTGGTCA
  AGGAGAAGCTGCGCCTTGATCCTGACAGCGAGATCGCCACCACCGGTGTGCGGGTGTCCCTCATCTGTCC
  GCTGGTGAAGATGCGGCTCTCCGTGCCCTGCCGGGCAGAGACCTGCGCCCACCTGCAGTGCTTCGACGCC
  GTCTTCTACCTGCAGATGAACGAGAAGAAGCCCACCTGGATGTGCCCCGTGTGCGACAAGCCAGCCCCCT
  ACGACCAGCTCATCATCGACGGGCTCCTCTCGAAGATCCTGAGCGAGTGTGAGGACGCCGACGAGATCGA
  GTACCTGGTGGACGGCTCGTGGTGCCCGATCCGCGCCGAAAAGGAGCGCAGCTGCAGCCCGCAGGGCGCC
  ATCCTCGTGCTGGGCCCCTCGGACGCCAATGGGCTCCTGCCCGCCCCCAGCGTCAACGGGAGCGGTGCCC
  TGGGCAGCACGGGTGGCGGCGGCCCGGTGGGCAGCATGGAGAATGGGAAGCCGGGCGCCGATGTGGTGGA
  CCTCACGCTGGACAGCTCATCGTCCTCGGAGGATGAGGAGGAGGAGGAAGAGGAGGAGGAAGACGAGGAC
  GAAGAGGGGCCCCGGCCCAAGCGCCGCTGCCCCTTCCAGAAGGGCCTGGTGCCGGCCTGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015897 unedited
  GGATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGCCAAGAGGCGG
  CGGAGCTGGTGGAGGCCAAAAACATGGTGATGAGTTTTCGAGTCTCCGACCTTCAGATGC
  TCCTGGGTTTCGTGGGCCGGAGTAAGAGTGGACTGAAGCACGAGCTCGTCACCAGGGCCC
  TCCAGCTGGTGCAGTTTGACTGTAGCCCTGAGCTGTTCAAGAAGATCAAGGAGCTGTACG
  AGACCCGCTACGCCAAGAAGAACTCGGAGCCTGCCCCACAGCCGCACCGGCCCCTGGACC
  CCCTGACCATGCACTCCACCTACGACCGGGCCGGCGCTGTGCCCAGGACTCCGCTGGCAG
  GCCCCAATATTGACTACCCCGTGCTCTACGGAAAGTACTTAAACGGACTGGGACGGTTGC
  CCGCCAAGACCCTCAAGCCAGAAGTCCGCCTGGTGAAGCTGCCGTTCTTTAATATGCTGG
  ATGAGCTGCTGAAGCCCACCGAATTAGTCCCACAGAACAACGAGAAGCTTCAGGAGAGCC
  CGTGCATCTTCGCATTGACGCCAAGACAGGTGGAGTTGATCCGGAACTCCAGGGAACTGC
  AGCCCGGAGTTAAAGCCGTGCAGGTCGTCCTGAGAATCTGTTACTCAGACACCAGCTGCC
  CTCAGGAGGACCAGTACCCGCCCAACATCGCTGTGAAGGTCAACCACAGCTACTGCTNCG
  TCCCGGGCTACTACCCCTCCAATAAGCCCGGGGGTGGAGCCCAAAGAGCCGTGCCGNCCC
  ATCAACCTCACTCACCTCATGTACCTGTCCTCGGCCACCACCGCATCACTGTCACCTGGG
  GAACTACNGCAGAGCTACTNCGTGGCCCTGTACCCTGTGCGCAGCTGACCTCATCGNAGC
  TGCTGCANAGCTGAAGACCATTGGGTAAAGCACCCGNACTGTGCAGGCACTGGTCAAGAG
  AGCTGCCCTTGATCTGACACGAGTCGCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015897 unedited
  AAAACCTTTTGCTTTTTTTTTCTATTCCCCCGCTTGGAAGTAGTCCAGTAGGTACAGGAT
  GCCAGGNAGCCAGTGCAAAAGGGGTGGGAAAAACAAAACGAACGACAATAAAAAGAAAAA
  GAAGGTCACTCCTCCCTCTGCGCCCGAGGCTGGCCCGTGCCCCTCTGCGTGGTGAGCACC
  AGGAAAGTCGAGTGTGCGGCCGGGGTCAGCAGGCCGGCACCAGGCCCTTCTGGAAGGGGC
  AGCGGCGCTTGGGCCGGGGCCCCTCTTCGTCCTCGTCTTCCTCCTCCTCTTCCTCCTCCT
  CCTCATCCTCCGAGGACGATGAGCTGTCCAGCGTGAGGTCCACCACATCGGCGCCCGGCT
  TCCCATTCTCCATGCTGCCCACCGGGCCGCCGCCACCCGTGCTGCCCAGGGCACCGCTCC
  CGTTGACGCTGGGGGCGGGCAGGAGCCCATTGGCGTCCGAGGGGCCCAGCACGAGGATGG
  CGCCCTGCGGGCTGCAGCTGCGCTCCTTTTCGGCGCGGATCGGGCACCACGAGCCGTCCA
  CCAGGTACTCGATCTCGTCGGCGTCCTCACACTCGCTCATGATCTTCGAGAGGAGCCCGT
  CGATGATGAGCTGGTCGTAGGGGGCTGGCTTGTCGCACACGGGGCACATCCAGGTGGGCT
  TCTTCTCGTTCATCTGCAGGTAGAAGACGGCGTCGAAGCACTGCAGGTGGGCGCAGGTCT
  CTGCCCGGCAGGGCACGGAGAGCCGCATCTTCACCAGCGGACAGATGATGGACACCCGCA
  CACCGGTGGTGGCGATCTCGCTGTCAGGATCAAGGCGCAGCTTCTCCTTGACCAGTGCCT
  TGCACAGCTCCGGGTGCTTTTACCCCATGGTCTTCAGCCTCTGCAGCAGCTCCGATGATG
  TCAGCTGCCGCACCAGGTACAGGGGCACCGAGTAGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015897
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015897.2, NP_056981.2
• RefSeq Size: 1835 bp
• RefSeq ORF: 1533 bp
• Locus ID: 51588
• UniProt ID: Q8N2W9
• Cytogenetics: 19p13.3
• Domains: SAP, zf-MIZ
• Protein Families: Transcription Factors
• Protein Pathways: Jak-STAT signaling pathway, Pathways in cancer, Small cell lung cancer, Ubiquitin mediated proteolysis
• Gene Summary: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.[UniProtKB/Swiss-Prot Function]