UCH37 (UCHL5) (NM_015984) Human Untagged Clone

CAT#: SC114560

UCHL5 (untagged)-Human ubiquitin carboxyl-terminal hydrolase L5 (UCHL5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UCH37
• Synonyms: CGI-70; INO80R; UCH-L5; UCH37
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015984 edited
  GAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGGTGTGGTTGACGAGCTCGGCG
  GCGGGTTTGCTGAGATCTGTGGCCGGCGGCAGCTGGTGCGGGGGGCAGCTGAGAGCGAGA
  GGTGGATCGGGGCGGTGTGTGGCCAGGGCCATGACGGGCAATGCCGGGGAGTGGTGCCTC
  ATGGAAAGCGACCCCGGGGTCTTCACCGAGCTCATTAAAGGATTCGGTTGCCGAGGAGCC
  CAAGTAGAAGAAATATGGAGTTTAGAGCCTGAGAATTTTGAAAAATTAAAGCCAGTTCAT
  GGGTTAATTTTTCTTTTCAAGTGGCAGCCAGGAGAAGAACCAGCAGGCTCTGTGGTTCAG
  GACTCCCGACTTGACACGATATTTTTTGCTAAGCAGGTAATTAATAATGCTTGTGCTACT
  CAAGCCATAGTGAGTGTGTTACTGAACTGTACCCACCAGGATGTCCATTTAGGCGAGACA
  TTATCAGAGTTTAAAGAATTTTCACAAAGTTTTGATGCAGCTATGAAAGGCTTGGCACTG
  AGCAATTCAGATGTGATTCGACAAGTACACAACAGTTTCGCCAGACAGCAAATGTTTGAA
  TTTGATACGAAGACATCAGCAAAAGAAGAAGATGCTTTTCACTTTGTCAGTTATGTTCCT
  GTTAATGGGAGACTGTATGAATTAGATGGATTAAGAGAAGGACCGATTGATTTAGGTGCA
  TGCAATCAAGATGATTGGATCAGTGCAGTAAGGCCTGTCATAGAAAAAAGGATACAAAAG
  TACAGTGAAGGTGAAATTCGATTTAATTTAATGGCCATTGTGTCTGACAGAAAAATGATA
  TATGAGCAGAAGATAGCAGAGTTACAAAGACAACTTGCAGAGGAGGAACCCATGGATACA
  GATCAAGGTAATAGTATGTTAAGTGCTATTCAGTCAGAAGTTGCCAAAAATCAGATGCTT
  ATTGAAGAAGAAGTACAGAAATTAAAAAGATACAAGATTGAGAATATCAGAAGGAAGCAT
  AATTATCTGCCTTTCATTATGGAATTGTTAAAGACTTTAGCAGAACACCAGCAGTTAATA
  CCACTAGTAGAAAAGGCAAAAGAAAAACAGAACGCAAAGAAAGCTCAGGAAACCAAATGA
  AGATGTTTTCAGATATGTACACATTTCTGCTTCTGCACATATTTTCATGGAAACCATTAT
  GTATAAAGAACTTAGAGCAACATCCTAATTGGCTCAGTGCACGTTTGGCAATAGTGCCAG
  CCTGTCTTGTCTTTAATGCATGGATTCATAAACTTCTTCCCTACCTGCATCATGTGCATG
  TAGTGCATATTAAATGAAAGTGATATTAAGAATGCTTTCCCAAATTCCATTATTTGACAT
  TGAGTCTGACAACTGTTAGTTTTCTGGTTGTCTAACTACCATATGAAGCTAGAAAATGCA
  CAAACGATATTCCTTATCTGTAATTTAAATACTTAAAATTTGCAATTGTCAGATCTTGAT
  TAAACTGGTTGTCTTATTTCTTCTCATCATTAACGGAAAAAAAATCAGTATTTCTATCTT
  TGATATCTAAGTGTTTTGAGGATTTTAAAACTGAATTTTATCTGCTATACCAGTTATTTG
  AGAAAGTATGATTTTAATGTAAATCATTTAAAAAGGACAAAAGTATAATTTCCAGTGATT
  TTCACTGCTGTCAGTAGAAAAGTAATAAACATCTCAATTTTATTTTAGTAAATTTTCTTC
  AAGTGTTTGGGGTTATTTGTTTATGTATTAGAGAATTGTTTCAGGAAGGTCTGAGTATTA
  TGGTTCAAAGCAAAATTTCAGGTTAAGAAGAAATTGTAAATCTTAAAGAATGTTGGTGTT
  ACTCTCAATGGAATATTGTTTCAAGCTTGTAAGCTGTGTATAAAAAAACTGGAGGTCTGA
  CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015984 unedited
  TATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCTCGTGCCGAATTCGGCAC
  GAGGGTGTGGTTGACGAGCTCGGCGGCGGGTTTGCTGAGATCTGTGGCCGGCGGCAGCTG
  GTGCGGGGGGCAGCTGAGAGCGAGAGGTGGATCGGGGCGGTGTGTGGCCAGGGCCATGAC
  GGGCAATGCCGGGGAGTGGTGCCTCATGGAAAGCGACCCCGGGGTCTTCACCGAGCTCAT
  TAAAGGATTCGGTTGCCGAGGAGCCCAAGTAGAAGAAATATGGAGTTTAGAGCCTGAGAA
  TTTTGAAAAATTAAAGCCAGTTCATGGGTTAATTTTTCTTTTCAAGTGGCAGCCAGGAGA
  AGAACCAGCAGGCTCTGTGGTTCAGGACTCCCGACTTGACACGATATTTTTTGCTAAGCA
  GGTAATTAATAATGCTTGTGCTACTCAAGCCATAGTGAGTGTGTTACTGAACTGTACCCA
  CCAGGATGTCCATTTANGCGAGACATTATCAGAGTTTAAAGAATTTTCACAAAGTTTTGA
  TGCAGCTATGANAGGCTTGGCACTGAGCAATTCAGATGTGATTCGANCAGTACACAACAG
  TTTCGCCAGACAGCANATGTTTGAATTTGATACGAAGACATCAGCAAAAGAAGAAGATGC
  TTTTCACTTTGTCAGNTATGTTCCTTTGTATGGGAGACTGTATGAATTANATGGATTAAG
  AGAAGACCCGATGATTTANNGTGCATGCATCAGATGATTGGATCAGTGCAGTANGGCCTG
  TCATANAAAAAGATACCANAGTACAGTGAAGGTGAATTCGATTTATTTAATGGGCCATTG
  TGTCTGACAGAAAATGATTTATGAGCAGAAGATAGCAGAGTTACAAAGACCACTTGCAGA
  GGGAGGAACCCATGGATACAGATCAAGGGAATAGTTTGTTAAGTGCTATTCAANNCNAAG
  TTGCCAAAATCAGATGCTTATGGNAGAANAGTNCNGANTTAAANGNATCCAGATGGGATT
  TCNGAGGGAGCTANNTTTTTGCTTTCTTTAGGNATGGTAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015984 unedited
  CTCCATTTTTTTAAACACGNCTTACAAGNCTTGAAACATATTCCATTGAGAGTAACACCA
  ACATTCTTTAAGATTTACAATTTCTTCTTAACCTGAATTTTGCTTTGAACCATAATACTC
  AGACCTTCCTGAAACAATTCTCTAATACATAAACAAATAACCCCAAACACTTGAAGAAAA
  TTTACTAAAATAAAATTGAGATGTTTATTACTTTTCTACTGACAGCAGTGAAAATCACTG
  GAAATTATACTTTTGTCCTTTTTAAATGATTTACATTAAAATCATACTTTCTCAAATAAC
  TGGTATAGCAGATAAAATTCAGTTTTAAAATCCTCAAAACACTTAGATATCAAAGATAGA
  AATACTGATTTTTTTTCCGTTAATGATGAGAAGAAATAAGACAACCAGTTTAATCAAGAT
  CTGACAATTGCAAATTTTAAGTATTTAAATTACAGATAAGGAATATCGTTTGTGCATTTT
  CTAGCTTCATATGGTAGTTAGACAACCAGAAAACTAACAGTTGTCAGACTCAATGTCAAA
  TAATGGAATTTGGGAAAGCATTCTTAATATCACTTTCATTTAATATGCACTACATGCACA
  TGATGCAGGTAGGGAAAGAAGTTTATGAATCCATGCATTAAAGACAAGACAGGCTGGCAC
  TATTGCCAAACGTGCACTGAGCCATTANGATGTTGCTCTAAAGTCTTATCATAATGGTTT
  TCATGAAATATGTGCAGAGCAGAAATGTGACATATCTGAAAAATCTCATTTGGTTNCTGA
  GCTTCTTTGCGTCTGGTTTTCTTTGGCTTTCTACTANGGGATAACGGGGGGGGTCGGCAA
  AGCTTAACAACCATATGAAAGCGAAATTTGCTCCTCGAGATCCAACTGGTATTTTAAACC
  GGCCTTCTTTAAACCACCGATTGGAACTTGACGAAACCCTAAAACTTACCGACGGTCAAG
  GGCCCTCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015984
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015984.1, NP_057068.1
• RefSeq Size: 1728 bp
• RefSeq ORF: 990 bp
• Locus ID: 51377
• UniProt ID: Q9Y5K5
• Cytogenetics: 1q31.2
• Domains: Peptidase_C12
• Protein Families: Druggable Genome, Protease
• Gene Summary: Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.