PIGT (NM_015937) Human Untagged Clone

CAT#: SC114534

PIGT (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class T (PIGT), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIGT
• Synonyms: CGI-06; MCAHS3; NDAP; PNH2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015937, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGGCTATGCCGCTTGCTCTGCTCGTCCTGTTGCTCCTGGGGCCCGGCGGCTGGTGCCTTGCAG
  AACCCCCACGCGACAGCCTGCGGGAGGAACTTGTCATCACCCCGCTGCCTTCCGGGGACGTAGCCGCCAC
  ATTCCAGTTCCGCACGCGCTGGGATTCGGAGCTTCAGCGGGAAGGAGTGTCCCATTACAGGCTCTTTCCC
  AAAGCCCTGGGGCAGCTGATCTCCAAGTATTCTCTACGGGAGCTGCACCTGTCATTCACACAAGGCTTTT
  GGAGGACCCGATACTGGGGGCCACCCTTCCTGCAGGCCCCATCAGGTGCAGAGCTGTGGGTCTGGTTCCA
  AGACACTGTCACTGATGTGGATAAATCTTGGAAGGAGCTCAGTAATGTCCTCTCAGGGATCTTCTGCGCC
  TCTCTCAACTTCATCGACTCCACCAACACAGTCACTCCCACTGCCTCCTTCAAACCCCTGGGTCTGGCCA
  ATGACACTGACCACTACTTTCTGCGCTATGCTGTGCTGCCGCGGGAGGTGGTCTGCACCGAAAACCTCAC
  CCCCTGGAAGAAGCTCTTGCCCTGTAGTTCCAAGGCAGGCCTCTCTGTGCTGCTGAAGGCAGATCGCTTG
  TTCCACACCAGCTACCACTCCCAGGCAGTGCATATCCGCCCTGTTTGCAGAAATGCACGCTGTACTAGCA
  TCTCCTGGGAGCTGAGGCAGACCCTGTCAGTTGTATTTGATGCCTTCATCACGGGGCAGGGAAAGAAAGA
  CTGGTCCCTCTTCCGGATGTTCTCCCGAACCCTCACGGAGCCCTGCCCCCTGGCTTCAGAGAGCCGAGTC
  TATGTGGACATCACCACCTACAACCAGGACAACGAGACATTAGAGGTGCACCCACCCCCGACCACTACAT
  ATCAGGACGTCATCCTAGGCACTCGGAAGACCTATGCCATCTATGACTTGCTTGACACCGCCATGATCAA
  CAACTCTCGAAACCTCAACATCCAGCTCAAGTGGAAGAGACCCCCAGAGAATGAGGCCCCCCCAGTGCCC
  TTCCTGCATGCCCAGCGGTACGTGAGTGGCTATGGGCTGCAGAAGGGGGAGCTGAGCACACTGCTGTACA
  ACACCCACCCATACCGGGCCTTCCCGGTGCTGCTGCTGGACACCGTACCCTGGTATCTGCGGCTGTATGT
  GCACACCCTCACCATCACCTCCAAGGGCAAGGAGAACAAACCAAGTTACATCCACTACCAGCCTGCCCAG
  GACCGGCTGCAACCCCACCTCCTGGAGATGCTGATTCAGCTGCCGGCCAACTCAGTCACCAAGGTTTCCA
  TCCAGTTTGAGCGGGCGCTGCTGAAGTGGACCGAGTACACGCCAGATCCTAACCATGGCTTCTATGTCAG
  CCCATCTGTCCTCAGCGCCCTTGTGCCCAGCATGGTAGCAGCCAAGCCAGTGGACTGGGAAGAGAGTCCC
  CTCTTCAACAGCCTGTTCCCAGTCTCTGATGGCTCTAACTACTTTGTGCGGCTCTACACGGAGCCGCTGC
  TGGTGAACCTGCCGACACCGGACTTCAGCATGCCCTACAACGTGATCTGCCTCACGTGCACTGTGGTGGC
  CGTGTGCTATGGCTCCTTCTACAATCTCCTCACCCGAACCTTCCACATCGAGGAGCCCCGCACAGGTGGC
  CTGGCCAAGCGGCTGGCCAACCTTATCCGGCGCGCCCGAGGTGTCCCCCCACTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015937 unedited
  CAGCATTTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGGGCTATGC
  CGCTTGCTCTGCTCGTCCTGTTGCTCCTGGGGCCCGGCGGCTGGTGCCTTGCAGAACCCC
  CACGCGACAGCCTGCGGGAGGAACTTGTCATCACCCCGCTGCCTTCCGGGGACGTAGCCG
  CCACATTCCAGTTCCGCACGCGCTGGGATTCGGAGCTTCAGCGGGAAGGAGTGTCCCATT
  ACAGGCTCTTTCCCAAAGCCCTGGGGCAGCTGATCTCCAAGTATTCTCTACGGGAGCTGC
  ACCTGTCATTCACACAAGGCTTTTGGAGGACCCGATACTGGGGGCCACCCTTCCTGCAGG
  CCCCATCAGGTGCAGAGCTGTGGGTCTGGTTCCAAGACACTGTCACTGATGTGGATAAAT
  CTTGGAAGGAGCTCAGTAATGTCCTCTCAGGGATCTTCTGCGCCTCTCTCAACTTCATCG
  ACTCCACCAACACAGTCACTCCCACTGCCTCCTTCAAACCCCTGGGTCTGGCCAATGACA
  CTGACCACTACTTTCTGCGCTATGCTGTGCTGCCGCGGGAAGGTGGTCTGCACCGAAAAC
  CTCACCCCCTGGAAGAAGCTCTTGCCCTGTAGTTCCAAGGCAGGCCTCTCTGTGCTGCTG
  AGGGCAGATCGCTTGTTCCACACCAGCTACCACTCCNANGCAGTGCATATNCGNNCCCTG
  TTGCAGAAAATGCACGCTGTACTAGCATCTNCTGGGAGCTGANGCAGACCCTGTCAGTNG
  TATTTGATGNCTTCATCACGGGGCAGGGAAAGAANNGACTGGTCCTCTNNNCCGNATGTT
  CTCCCGACCCTNACGGAGCCNTGCCCCCTGGCTTANAGAANCGAGTCTATGGTGCANTNC
  CNCCTACACCAGGACACGAACATTTAAAGGTGCCCCCACCCGACACTACTTANAGGGAGT
  NATCTAGACTTCGGAGACATAGCATTATGACTGGTTGACCGCCTGTCAACACTTGAACTA
  ATCACTAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015937 unedited
  TCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTCACGGAAACAGCCGTTTTTATTCCACAAA
  CAGTAAACTCCATCCACTACACAGTGACATACACTGTGTAGCACACCCCATCACCTTGGC
  CAGTGCTGCCAGCCGACCTTTCTGTGGTGATGGAAATCTTTTTCTGTGCTGTCCAATACA
  GCAGCCACCGACCACTTTTGCTTATTGAGCACCTCAATATAGAGGTGGCCACTACAGGTG
  AGGAAATGAATTTCTAAGTTAATTCAAATTCAAATGCCACATTTGGCTCGTGGCTCCTGT
  ACTGGACAACACAGCTGTAGGCCCTGACCTGGTCCAGGGCACTTTGGTTCAAAAGCCAAC
  TCTGAGGAGAGCAAGTGGCAGAAACAGCCCTTGGGCTCCCCTCCCCAGAGAGAAACGGCA
  GCTGCAGCTGCTGGAAAGGGCAAGAATCAGAGTGGGGGGACACCTCGGGCGCGCCGGATA
  AGGTTGGCCAGCCGCTTGGCCAGGCCACCTGTGCGGGGCTCCTCGATGTGGAAGGTTCGG
  GTGAGGAGATTGTAAAAGGAGCCGTANCACACGGCCACCACAGTGCACGTGAGGCANATC
  ACGTTGTAGGGCATGCTGAGTCCCGTGTCNGCAGGTCACCACCAGCGGCTCCGTGTAGAG
  CCGCACAAAGTATTAGAGCCATCANAGACTGGGAACAAGCTGTTGAAGAGGGGACTCTCT
  TCCCAGCCACTGCCTTGGCTGCTACATGCTGGCCACAAGGCCCTGAAGACAAAGGGCTGA
  CCTAAACCCATGTTAGGATCTGGGGGTACTTGGCACTTAACAAGCCCCGTCAACTGGAAG
  GAACCTCGGGACCGATTGGCCGCAATTTATCACACTTCCGAAGGGGGGTGCACCCGGCCT
  GCACGCCGGAAAGGAGTACCGGCTGCTCCCTGCCCTGGAGGAGGAGGGGGGCCAACGCCA
  CAACCAGGCCGGGCCAACAAACCGGAAGCCGGCTGGGGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015937
• Insert Size: 2260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015937.1, NP_057021.1
• RefSeq Size: 2246 bp
• RefSeq ORF: 1725 bp
• Locus ID: 51604
• UniProt ID: Q969N2
• Cytogenetics: 20q13.12
• Domains: Gpi16
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• Gene Summary: This gene encodes a protein that is involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This protein is an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).