EPLIN (LIMA1) (NM_016357) Human Untagged Clone

CAT#: SC114337

LIMA1 (untagged)-Human LIM domain and actin binding 1 (LIMA1), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EPLIN
• Synonyms: EPLIN; LDLCQ8; SREBP3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114337 sequence for NM_016357 edited (data generated by NextGen Sequencing)
  ATGGAATCATCTCCATTTAATAGACGGCAATGGACCTCACTATCATTGAGGGTAACAGCC
  AAAGAACTTTCTCTTGTCAACAAGAACAAGTCATCGGCTATTGTGGAAATATTCTCCAAG
  TACCAGAAAGCAGCTGAAGAAACAAACATGGAGAAGAAGAGAAGTAACACCGAAAATCTC
  TCCCAGCACTTTAGAAAGGGGACCCTGACTGTGTTAAAGAAGAAGTGGGAGAACCCAGGG
  CTGGGAGCAGAGTCTCACACAGACTCTCTACGGAACAGCAGCACTGAGATTAGGCACAGA
  GCAGACCATCCTCCTGCTGAAGTGACAAGCCACGCTGCTTCTGGAGCCAAAGCTGACCAA
  GAAGAACAAATCCACCCCAGATCTAGACTCAGGTCACCTCCTGAAGCCCTCGTTCAGGGT
  CGATATCCCCACATCAAGGACGGTGAGGATCTTAAAGACCACTCAACAGAAAGTAAAAAA
  ATGGAAAATTGTCTAGGAGAATCCAGGCATGAAGTAGAAAAATCAGAAATCAGTGAAAAC
  ACAGATGCTTCGGGCAAAATAGAGAAATATAATGTTCCGCTGAACAGGCTTAAGATGATG
  TTTGAGAAAGGTGAACCAACTCAAACTAAGATTCTCCGGGCCCAAAGCCGAAGTGCAAGT
  GGAAGGAAGATCTCTGAAAACAGCTATTCTCTAGATGACCTGGAAATAGGCCCAGGTCAG
  TTGTCATCTTCTACATTTGACTCGGAGAAAAATGAGAGTAGACGAAATCTGGAACTTCCA
  CGCCTCTCAGAAACCTCTATAAAGGATCGAATGGCCAAGTACCAGGCAGCTGTGTCCAAA
  CAAAGCAGCTCAACCAACTATACAAATGAGCTGAAAGCCAGTGGTGGCGAAATCAAAATT
  CATAAAATGGAGCAAAAGGAGAATGTGCCCCCAGGTCCTGAGGTCTGCATCACCCATCAG
  GAAGGGGAAAAGATTTCTGCAAATGAGAATAGCCTGGCAGTCCGTTCCACCCCTGCCGAA
  GATGACTCCCGTGACTCCCAGGTTAAGAGTGAGGTTCAACAGCCTGTCCATCCCAAGCCA
  CTAAGTCCAGATTCCAGAGCCTCCAGTCTTTCTGAAAGTTCTCCTCCCAAAGCAATGAAG
  AAGTTTCAGGCACCTGCAAGAGAGACCTGCGTGGAATGTCAGAAGACAGTCTATCCAATG
  GAGCGTCTCTTGGCCAACCAGCAGGTGTTTCACATCAGCTGCTTCCGTTGCTCCTATTGC
  AACAACAAACTCAGTCTAGGAACATATGCATCTTTACATGGAAGAATCTATTGTAAGCCT
  CACTTCAATCAACTCTTTAAATCTAAGGGCAACTATGATGAAGGCTTTGGGCACAGACCA
  CACAAGGATCTATGGGCAAGCAAAAATGAAAACGAAGAGATTTTGGAGAGACCAGCCCAG
  CTTGCAAATGCAAGGGAGACCCCTCACAGCCCAGGGGTAGAAGATGCCCCTATTGCTAAG
  GTGGGTGTCCTGGCTGCAAGTATGGAAGCCAAGGCCTCCTCTCAGCAGGAGAAGGAAGAC
  AAGCCAGCTGAAACCAAGAAGCTGAGGATCGCCTGGCCACCCCCCACTGAACTTGGAAGT
  TCAGGAAGTGCCTTGGAGGAAGGGATCAAAATGTCAAAGCCCAAATGGCCTCCTGAAGAC
  GAAATCAGCAAGCCCGAAGTTCCTGAGGATGTCGATCTAGATCTGAAGAAGCTAAGACGA
  TCTTCTTCACTGAAGGAAAGAAGCCGCCCATTCACTGTAGCAGCTTCATTTCAAAGCACC
  TCTGTCAAGAGCCCAAAAACTGTGTCCCCACCTATCAGGAAAGGCTGGAGCATGTCAGAG
  CAGAGTGAAGAGTCTGTGGGTGGAAGAGTTGCAGAAAGGAAACAAGTGGAAAATGCCAAG
  GCTTCTAAGAAGAATGGGAATGTGGGAAAAACAACCTGGCAAAACAAAGAATCTAAAGGA
  GAGACAGGGAAGAGAAGTAAGGAAGGTCATAGTTTGGAGATGGAGAATGAGAATCTTGTA
  GAAAATGGTGCAGACTCCGATGAAGATGATAACAGCTTCCTCAAACAACAATCTCCACAA
  GAACCCAAGTCTCTGAATTGGTCGAGTTTTGTAGACAACACCTTTGCTGAAGAATTCACT
  ACTCAGAATCAGAAATCCCAGGATGTGGAACTCTGGGAGGGAGAAGTGGTCAAAGAGCTC
  TCTGTGGAAGAACAGATAAAGAGAAATCGGTATTATGATGAGGATGAGGATGAAGAGTGA
  
  Clone variation with respect to NM_016357.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016357 unedited
  GTCGGGATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGAGACAAGATG
  GAATCATCTCCATTTAATAGACGGCAATGGACCTCACTATCATTGAGGGTAACAGCCAAA
  GAACTTTCTCTTGTCAACAAGAACAAGTCATCGGCTATTGTGGAAATATTCTCCAAGTAC
  CAGAAAGCAGCTGAAGAAACAAACATGGAGAAGAAGAGAAGTAACACCGAAAATCTCTCC
  CAGCACTTTAGAAAGGGGACCCTGACTGTGTTAAAGAAGAAGTGGGAGAACCCAGGGCTG
  GGAGCAGAGTCTCACACAGACTCTCTACGGAACAGCAGCACTGAGATTAGGCACAGAGCA
  GACCATCCTCCTGCTGAAGTGACAAGCCACGCTGCTTCTGGAGCCAAAGCTGACCAAGAA
  GAACAAATCCACCCCAGATCTAGACTCAGGTCACCTCCTGAAGCCCTCGTTCAGGGTCGA
  TATCCCCACATCAAGGACGGTGAGGATCTTAAAGACCACTCAACAGAAAGTAAAAAAATG
  GAAAATTGTCTAGGAGAATCCAGGCATGAAGTAGAAAAATCAGAAATCAGTGAAAACACA
  GATGCTTCGGGCAAAATAGAGAAATATAATGTTCCGCTGAACAGGCTTAAGATGATGTTT
  GAGAAAGGTGAACCAACTCANACTAAGATTCTCCGGGCCCAAAGCCGAAGTGCAAGTGGA
  AGGAAGATCTCTNGANACAGCTATTCTCTAGATGACCTGGNAATANGCCCCAGTCAGNTG
  TCATCTTCTACATTTGACTCGGAGAAAAATGAGAGTAGACGAAATCTGGAACTTCCACGC
  CTCTCAGAACCTCTATAAAGATCGAATGGCCAGTACCAGCAGCTGTGTCCAAACAAGCAG
  CTCAACCACTATACCAATGACCTGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016357 unedited
  CGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGATTTTAAAAAGGAATTCT
  TTTCCAAAGTTACTTCCAAGTAAATTACATTTCATGCTGGGATACCTGCTTATGTGCATC
  ACATTTTGACAAAGGGCAGTGGCTCGCTAACACTAACATGAATTTAAGGCCCAGCATCAT
  TGCAATTTGTCACTCTTCATCCTCATCCTCATCATAATACCGATTTCTCTTTATCTGTTC
  TTCCACAGAGAGCTCTTTGACCACTTCTCCCTCCCAGAGTTCCACATCCTGGGATTTCTG
  ATTCTGAGTAGTGAATTCTTCAGCAAAGGTGTTGTCTACAAAACTCGACCAATTCAGAGA
  CTTGGGTTCTTGTGGAGATTGTTGTTTGAGGAAGCTGTTATCATCTTCATCGGAGTCTGC
  ACCATTTTCTACAAGATTCTCATTCTCCATCTCCAAACTATGACCTTCCTTACTTCTCTT
  CCCTGTCTCTCCTTTAGATTCTTTGTTTTGCCAGGTTGTTTTTCCCACATTCCCATTCTT
  CTTAGAAGCCTTGGCATTTTCCACTTGTTTCCTTTCTGCAACTCTTCCACCCACAGACTC
  TTCACTCTGCTCTGACATGCTCCAGCCTTTCCTGATAGGTGGGGACACAGTTTTTGGGCT
  CTTGACAGANGTGCTNTGAAATGAAGCTGCTACAGTGAATGGGCGGCTTCTTTCCTTCAG
  TGAAGAAGATCGTCTTAGCTTCTTCAGATCTAGATCGACATCCTCAGGAACTTCGGGCTT
  GCTGATTTCGTCTTCAGGAGCCATTTNGGGCTTGACATTNTGATCCCTTCTNCANNGCAC
  TNNCTGACTNNCAGTTCAGTGGNGGTGGCCAGGCGATCCTCACTTCTGGNTCAGCTGGCT
  GCTTCCTCTCTGCGAAAGAGGCCTGCTTCATACTGANNCAGAACCACTACAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016357
• Insert Size: 2360 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016357.3, NP_057441.1
• RefSeq Size: 3664 bp
• RefSeq ORF: 2280 bp
• Locus ID: 51474
• UniProt ID: Q9UHB6
• Cytogenetics: 12q13.12
• Domains: LIM
• Gene Summary: This gene encodes a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles. It is downregulated in some cancer cell lines. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and expression of some of the variants maybe independently regulated. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site at an internal coding exon compared to variant 1. This results in an isoform (2, also known as EPLIN-beta) that is 1 aa shorter than isoform 1.