UBP43 (USP18) (NM_017414) Human Untagged Clone

CAT#: SC114102

USP18 (untagged)-Human ubiquitin specific peptidase 18 (USP18)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBP43
• Synonyms: ISG43; PTORCH2; UBP43
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_017414 edited
  GAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGCTGGAAGTCTGGAGCAGGTGC
  GCGGCTGCAACGGCAGCCGCGGGAAGCTCGGGCCGGCAGGGTTTCCCCGCACGCTGGCGC
  CCAGCTCCCGGCGCGGAGGCCGCTGTAAGTTTCGCTTTCCATTCAGTGGAAAACGAAAGC
  TGGGCGGGGTGCCACGAGCGCGGGGCCAGACCAAGGCGGGCCCGGAGCGGAACTTCGGTC
  CCAGCTCGGTCCCCGGCTCAGTCCCGACGTGGAACTCAGCAGCGGAGGCTGGACGCTTGC
  ATGGCGCTTGAGAGATTCCATCGTGCCTGGCTCACATAAGCGCTTCCTGGAAGTGAAGTC
  GTGCTGTCCTGAACGCGGGCCAGGCAGCTGCGGCCTGGGGGTTTTGGAGTGATCACGAAT
  GAGCAAGGCGTTTGGGCTCCTGAGGCAAATCTGTCAGTCCATCCTGGCTGAGTCCTCGCA
  GTCCCCGGCAGATCTTGAAGAAAAGAAGGAAGAAGACAGCAACATGAAGAGAGAGCAGCC
  CAGAGAGCGTCCCAGGGCCTGGGACTACCCTCATGGCCTGGTTGGTTTACACAACATTGG
  ACAGACCTGCTGCCTTAACTCCTTGATTCAGGTGTTCGTAATGAATGTGGACTTCACCAG
  GATATTGAAGAGGATCACGGTGCCCAGGGGAGCTGACGAGCAGAGGAGAAGCGTCCCTTT
  CCAGATGCTTCTGCTGCTGGAGAAGATGCAGGACAGCCGGCAGAAAGCAGTGCGGCCCCT
  GGAGCTGGCCTACTGCCTGCAGAAGTGCAACGTGCCCTTGTTTGTCCAACATGATGCTGC
  CCAACTGTACCTCAAACTCTGGAACCTGATTAAGGACCAGATCACTGATGTGCACTTGGT
  GGAGAGACTGCAGGCCCTGTATACGATCCGGGTGAAGGACTCCTTGATTTGCGTTGACTG
  TGCCATGGAGAGTAGCAGAAACAGCAGCATGCTCACCCTCCCACTTTCTCTTTTTGATGT
  GGACTCAAAGCCCCTGAAGACACTGGAGGACGCCCTGCACTGCTTCTTCCAGCCCAGGGA
  GTTATCAAGCAAAAGCAAGTGCTTCTGTGAGAACTGTGGGAAGAAGACCCGTGGGAAACA
  GGTCTTGAAGCTGACCCATTTGCCCCAGACCCTGACAATCCACCTCATGCGATTCTCCAT
  CAGGAATTCACAGACGAGAAAGATCTGCCACTCCCTGTACTTCCCCCAGAGCTTGGATTT
  CAGCCAGATCCTTCCAATGAAGCGAGAGTCTTGTGATGCTGAGGAGCAGTCTGGAGGGCA
  GTATGAGCTTTTTGCTGTGATTGCGCACGTGGGAATGGCAGACTCCGGTCATTACTGTGT
  CTACATCCGGAATGCTGTGGATGGAAAATGGTTCTGCTTCAATGACTCCAATATTTGCTT
  GGTGTCCTGGGAAGACATCCAGTGTACCTACGGAAATCCTAACTACCACTGGCAGGAAAC
  TGCATATCTTCTGGTTTACATGAAGATGGAGTGCTAATGGAAATGCCCAAAACCTTCAGA
  GATTGACACGCTGTCATTTTCCATTTCCGTTCCTGGATCTACGGAGTCTTCTAAGAGATT
  TTGCAATGAGGAGAAGCATTGTTTTCAAACTATATAACTGAGCCTTATTTATAATTAGGG
  ATATTATCAAAATATGTAACCATGAGGCCCCTCAGGTCCTGATCAGTCAGAATGGATGCT
  TTCACCAGCAGACCCGGCCATGTGGCTGCTCGGTCCTGGGTGCTCGCTGCTGTGCAAGAC
  ATTAGCCCTTTAGTTATGAGCCTGTGGGAACTTCAGGGGTTCCCAGTGGGGAGAGCAGTG
  GCAGTGGGAGGCATCTGGGGGCCAAAGGTCAGTGGCAGGGGGTATTTCAGTATTATACAA
  CTGCTGTGACCAGACTTGTATACTGGCTGAATATCAGTGCTGTTTGTAATTTTTCACTTT
  GAGAACCAACATTAATTCCATATGAATCAAGTGTTTTGTAACTGCTATTCATTTATTCAG
  CAAATATTTATTGATCATCTCTTCTCCATAAGATAGTGTGATAAACACAGTCATGAATAA
  AGTTATTTTCCACAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_017414 unedited
  NTGTTCAGATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCTCGTGC
  CGAATTCGGCACGAGGCTGGAAGTCTGGAGCAGGTGCGCGGCTGCAACGGCAGCCGCGGG
  AAGCTCGGGCCGGCAGGGTTTCCCCGCACGCTGGCGCCCAGCTCCCGGCGCGGAGGCCGC
  TGTAAGTTTCGCTTTCCATTCAGTGGAAAACGAAAGCTGGGCGGGGTGCCACGAGCGCGG
  GGCCAGACCAAGGCGGGCCCGGAGCGGAACTTCGGTCCCAGCTCGGTCCCCGGCTCAGTC
  CCGACGTGGAACTCAGCAGCGGAGGCTGGACGCTTGCATGGCGCTTGAGAGATTCCATCG
  TGCCTGGCTCACATAAGCGCTTCCTGGAAGTGAAGTCGTGCTGTCCTGAACGCGGGCCAG
  GCAGCTGCGGCCTGGGGGTTTTGGAGTGATCACGAATGAGCAAGGCGTTTGGGCTCCTGA
  GGCAAATCTGTCAGTCCATCCTGGCTGAGTCCTCGCAGTCCCCGGCAGATCTTGAAGAAA
  AGAAGGAAGAAGACAGCAACATGAAGAGAGAGCAGCCCAGAGAGCGTCCCAGGGCCTGGG
  ACTACCCTCATGGCCTGGTTGGTTTACACAACATTGGACAGACCTGCTGCCTTAACTCCT
  TGATTCAGGTGTTCGTAATGAATGTGGACTTCACCAGGATATTGAAGAGGATCACGGTGC
  CCAGNGGAGCTGACGAGCAGAGGAGAAGCGTCCCTTTCCAGATGCTTCTGCTGCTGGAGA
  AGATGCANGACAGCCGGCAGAAAGCAGTGCGGCCCCTGGAGCTGGCCTACTGCCTGCAGA
  AGTGCAACGTGCCCTTGNNTTGTCCACATGATGCTGCCCAACTGTACCTCANAACTCTGG
  AACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_017414 unedited
  NACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGTGGAAAATAACTTT
  ATTCATGACTGTGTTTATCACACTATCTTATGGAGAAGAGATGATCAATAAATATTTGCT
  GAATAAATGAATAGCAGTTACAAAACACTTGATTCATATGGAATTAATGTTGGTTCTCAA
  AGTGAAAAATTACAAACAGCACTGATATTCAGCCAGTATACAAGTCTGGTCACAGCAGTT
  GTATAATACTGAAATACCCCCTGCCACTGACCTTTGGCCCCCAGATGCCTCCCACTGCCA
  CTGCTCTCCCCACTGGGAACCCCTGAAGTTCCCACAGGCTCATAACTAAAGGGCTAATGT
  CTTGCACAGCAGCGAGCACCCAGGACCGAGCAGCCACATGGCCGGGTCTGCTGGTGAAAG
  CATCCATTCTGACTGATCAGGACCTGAGGGGCCTCATGGTTACATATTTTGATAATATCC
  CTAATTATAAATAAGGCTCAGTTATATAGTTTGAAAACAATGCTTCTCCTCATTGCAAAA
  TCTCTTAGAAGACTCCGTAGATCCAGGAACGGAAATGGAAAATGACAGCGTGTCAATCTC
  TGAAGGTTTTGGGCATTTCCATTAGCACTCCATCTTCATGTAAACCAGAAGATATGCAGT
  TTCCTGCCAGTGGTAGTTAGGATTTCCGTAGGTACACTGGATGTCTTCCCAGGACACCAA
  GCAAATATTGGAGTCATTGAAGCAGAACCATTTTCCATTCACAGCATTCCGGATGTAGAA
  CAGTAATGACCGGAGTCTGCCATTCCCACGTGCGCAATCACAGCAAAAGCTCATACTGGC
  CTTCAGACTGCTNCTCAGCATCACAAGACTCTCGCTTCATTGGAAAGATCTGGCTGAAAT
  CCAGCTCTGGGGGAAGTCAAGGAGTGCAGAATCTTCTTCTCTGTGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_017414
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017414.2, NP_059110.2
• RefSeq Size: 2054 bp
• RefSeq ORF: 1119 bp
• Locus ID: 11274
• UniProt ID: Q9UMW8
• Cytogenetics: 22q11.21
• Domains: UCH
• Protein Families: Druggable Genome, Protease
• Gene Summary: The protein encoded by this gene belongs to the ubiquitin-specific proteases (UBP) family of enzymes that cleave ubiquitin from ubiquitinated protein substrates. It is highly expressed in liver and thymus, and is localized to the nucleus. This protein efficiently cleaves only ISG15 (a ubiquitin-like protein) fusions, and deletion of this gene in mice results in a massive increase of ISG15 conjugates in tissues, indicating that this protein is a major ISG15-specific protease. Mice lacking this gene are also hypersensitive to interferon, suggesting a function of this protein in downregulating interferon responses, independent of its isopeptidase activity towards ISG15. [provided by RefSeq, Sep 2011]