WDR12 (NM_018256) Human Untagged Clone

CAT#: SC113619

WDR12 (untagged)-Human WD repeat domain 12 (WDR12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WDR12
• Synonyms: YTM1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018256, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTCAGCTCCAAACACGCTTCTACACTGATAACAAGAAATATGCCGTAGATGATGTTCCCTTCTCAA
  TCCCTGCTGCCTCTGAAATTGCCGACCTTAGTAACATCATCAATAAACTACTAAAGGACAAAAATGAGTT
  CCACAAACATGTGGAGTTTGATTTCCTTATTAAGGGCCAGTTTCTGCGAATGCCCTTGGACAAACACATG
  GAAATGGAGAACATCTCATCAGAAGAAGTTGTGGAAATAGAATACGTGGAGAAGTATACTGCACCCCAGC
  CAGAGCAATGCATGTTCCATGATGACTGGATCAGTTCAATTAAAGGGGCAGAGGAATGGATCTTGACTGG
  TTCTTATGATAAGACTTCTCGGATCTGGTCCTTGGAAGGAAAGTCAATAATGACAATTGTGGGACATACG
  GATGTTGTAAAAGATGTGGCCTGGGTGAAAAAAGATAGTTTGTCCTGCTTATTATTGAGTGCTTCTATGG
  ATCAGACTATTCTCTTATGGGAGTGGAATGTAGAGAGAAACAAAGTGAAAGCCCTACACTGCTGTAGAGG
  TCATGCTGGAAGTGTAGATTCTATAGCTGTTGATGGCTCAGGAACTAAATTTTGCAGTGGCTCCTGGGAT
  AAGATGCTAAAGATCTGGTCTACAGTCCCTACAGATGAAGAAGATGAAATGGAGGAGTCCACAAATCGAC
  CAAGAAAGAAACAGAAGACAGAACAGTTGGGACTAACAAGGACTCCCATAGTGACCCTCTCTGGCCACAT
  GGAGGCAGTTTCCTCAGTTCTGTGGTCAGATGCTGAAGAAATCTGCAGTGCATCTTGGGACCATACAATT
  AGAGTGTGGGATGTTGAGTCTGGCAGTCTTAAGTCAACTTTGACAGGAAATAAAGTGTTTAATTGTATTT
  CCTATTCTCCACTTTGTAAACGTTTAGCATCTGGAAGCACAGATAGGCATATCAGACTGTGGGATCCCCG
  AACTAAAGATGGTTCTTTGGTGTCGCTGTCCCTAACGTCACATACTGGTTGGGTGACATCAGTAAAATGG
  TCTCCTACCCATGAACAGCAGCTGATTTCAGGATCTTTAGATAACATTGTTAAGCTGTGGGATACAAGAA
  GTTGTAAGGCTCCTCTCTATGATCTGGCTGCTCATGAAGACAAAGTTCTGAGTGTAGACTGGACAGACAC
  AGGGCTACTTCTGAGTGGAGGAGCAGACAATAAATTGTATTCCTACAGATATTCACCTACCACTTCCCAT
  GTTGGGGCATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018256 unedited
  GGTCAGGATTTGTATACGATTTATATAGGCGGCCGCGNAATTCGCACGAGGCCGCAGTTA
  GAGGCGAGATTTAGGAAAAACCTCTGCCGAGTGAGCCTCTGGTTGGGAATATGTNATGAG
  AAAAAAAAACTGGCAAGGCGTTAGTCAAGCAAAGCTGAAGGCAGAGGAAATTTGATATCT
  GGCTGGAGTCTAGAGGATTTAATGCAAATAAGATACTCTGAGGGCAGCGTGGCAAAAAAA
  GACTACAATTCCCGGTGGTCACAGCGTTTGAGAAGCGATGCTTTCTGAGACTTGTAGTAA
  CTAGGAGCTGTGTTTGAACTATCCAGGCTCAGGACAGCCTCTTGAAAAAAAATTTTTTAT
  TAATAAAGCGGATTTGAGTGGGATCTTTTTCCTAATCGATTACGGGCCCACACGTATGGG
  AAGAATTCTAACAATGATTAAAGGGACATGCTACCTTTACGACTATCCTTTTCTAATCGA
  TGACTCCTAAATCTAGGAGTAGGTAGTCGATGTTTGTGGTCTGGGCGTCTGTAGAAGGGC
  AACCTCGTGCTTTCTGCAGAGGAGACCGGAGGGCAGAAGGCAGAGTCCAGGCTTAGACTG
  CAGTTCCTCGCTTACCTGTGCAGTCTAATTTTGAGCTGCCTCTTTGTAGTCTTAAAAGGC
  AGGAGCTTCGTGTTGTGGGTCTGCTAACCCGTACGTTTCCGTGGGCAAGTCGTGTGTACT
  CCTCGCCATGGGCTCAGCTCCAACACGCTTCTACACTGATAACAAGAAATATGCCGTAGA
  TGATGTTNCCTTCTCAATCCTGCTGCCTCTGAAATGCCGACCTTTAGTACATCATNNCAT
  AACTACTAAAGGACAAAATGTAGTCCACAAACATGGGNAGTTTGATTTCCTTATTAAGGG
  CCAGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018256 unedited
  GGGCCTGGGGTGAACTAGCCTCCCTCCAGTTTGTATAAAAACGTATGCCACANTTTGTAT
  TTTATTTACACAACCTATCTTATTATAAATACCAAATAAGAAAAAGTGATACGTTAGCTG
  TTATGAAGGGTGAAAACATTATATAAACTTCAAAAGGCTGCTTTCTGCATCTGCATCTAT
  GTAATTTCATGGTTCTCTACCAATTTCATTTACAGAAANATTCNTTATAGTCAAATTATT
  GTTCACTTTCATGCCCCAACATGGGAAGTGGTAGGTGAATATCTGTAGGAATACAATTTA
  TTGTCTGCTCCTCCACTCAGAAGTAGCCCTGTGTCTGTCCAGTCTACACTCAGAACTTTG
  TCTTCATGAGCAGCCAGATCATAGAGAGGAGCCTTACAACTTCTTGTATCCCACAGCTTA
  ACAATGTTATCTAAAGATCCTGAAATCAGCTGCTGTTCATGGGTAGGAGACCATTTTACT
  GATGTCACCCAACCAGTATGTGACGTTAGGGACAGCGACACCAAAGAACCATCTTTAGTT
  CGGGGATCCCACAGTCTGATATGCCTATCTGTGCTTCCAGATGCTAAACGTTTACAAAGT
  GGAGAATAGGAAATACAATTAAACACTTTATTTCCTGTCAAAGTTGACTTAAGACTGCCA
  GACTCAACGTCCCACACTCTAATTGTATGGTCCCAAGATGCACTGCAGATTTCTTCAGCA
  TCTGACCACAGAACTGATGAAACTGCCTCCATGTGGCCAGAGAGGGTCACTATGGGAGTC
  CTTGTTAGTCCCAACTGTTCTGTCTTCTGTTTCTTTCTTGGTCGATTGTGGACTCCTCCA
  TTTCTCTTCTTCATCTGTAGGGACTGTAGACCAGATCTTTAGCTCTTATCCAGGACCCCT
  GCAAAATTAATTCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018256
• Insert Size: 2500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018256.2, NP_060726.2
• RefSeq Size: 1720 bp
• RefSeq ORF: 1272 bp
• Locus ID: 55759
• UniProt ID: Q9GZL7
• Cytogenetics: 2q33.2
• Domains: WD40
• Gene Summary: This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein is highly similar to the mouse WD repeat domain 12 protein at the amino acid level. The protein encoded by this gene is a component of a nucleolar protein complex that affects maturation of the large ribosomal subunit.[provided by RefSeq, Dec 2008]