SPHK2 (NM_020126) Human Untagged Clone

CAT#: SC113181

SPHK2 (untagged)-Human sphingosine kinase 2 (SPHK2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPHK2
• Synonyms: SK-2; SK 2; SPK-2; SPK 2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113181 sequence for NM_020126 edited (data generated by NextGen Sequencing)
  ATGAATGGACACCTTGAAGCAGAGGAGCAGCAGGACCAGAGGCCAGACCAGGAGCTGACC
  GGGAGCTGGGGCCACGGGCCTAGGAGCACCCTGGTCAGGGCTAAGGCCATGGCCCCGCCC
  CCACCGCCACTGGCTGCCAGCACCCCGCTCCTCCATGGCGAGTTTGGCTCCTACCCAGCC
  CGAGGCCCACGCTTTGCCCTCACCCTTACATCGCAGGCCCTGCACATACAGCGGCTGCGC
  CCCAAACCTGAAGCCAGGCCCCGGGGTGGCCTGGTCCCGTTGGCCGAGGTCTCAGGCTGC
  TGCACCCTGCGAAGCCGCAGCCCCTCAGACTCAGCGGCCTACTTCTGCATCTACACCTAC
  CCTCGGGGCCGGCGCGGGGCCCGGCGCAGAGCCACTCGCACCTTCCGGGCAGATGGGGCC
  GCCACCTACGAAGAGAACCGTGCCGAGGCCCAGCGCTGGGCCACTGCCCTCACCTGTCTG
  CTCCGAGGACTGCCACTGCCCGGGGATGGGGAGATCACCCCTGACCTGCTACCTCGGCCG
  CCCCGGTTGCTTCTATTGGTCAATCCCTTTGGGGGTCGGGGCCTGGCCTGGCAGTGGTGT
  AAGAACCACGTGCTTCCCATGATCTCTGAAGCTGGGCTGTCCTTCAACCTCATCCAGACA
  GAACGACAGAACCACGCCCGGGAGCTGGTCCAGGGGCTGAGCCTGAGTGAGTGGGATGGC
  ATCGTCACGGTCTCGGGAGACGGGCTGCTCCATGAGGTGCTGAACGGGCTCCTAGATCGC
  CCTGACTGGGAGGAAGCTGTGAAGATGCCTGTGGGCATCCTCCCCTGCGGCTCGGGCAAC
  GCGCTGGCCGGAGCAGTGAACCAGCACGGGGGATTTGAGCCAGCCCTGGGCCTCGACCTG
  TTGCTCAACTGCTCACTGTTGCTGTGCCGGGGTGGTGGCCACCCACTGGACCTGCTCTCC
  GTGACGCTGGCCTCGGGCTCCCGCTGTTTCTCCTTCCTGTCTGTGGCCTGGGGCTTCGTG
  TCAGATGTGGATATCCAGAGCGAGCGCTTCAGGGCCTTGGGCAGTGCCCGCTTCACACTG
  GGCACGGTGCTGGGCCTCGCCACACTGCACACCTACCGCGGACGCCTCTCCTACCTCCCC
  GCCACTGTGGAACCTGCCTCGCCCACCCCTGCCCATAGCCTGCCTCGTGCCAAGTCGGAG
  CTGACCCTAACCCCAGACCCAGCCCCGCCCATGGCCCACTCACCCCTGCATCGTTCTGTG
  TCTGACCTGCCTCTTCCCCTGCCCCAGCCTGCCCTGGCCTCTCCTGGCTCGCCAGAACCC
  CTGCCCATCCTGTCCCTCAACGGTGGGGGCCCAGAGCTGGCTGGGGACTGGGGTGGGGCT
  GGGGATGCTCCGCTGTCCCCGGACCCACTGCTGTCTTCACCTCCTGGCTCTCCCAAGGCA
  GCTCTACACTCACCCGTCTCCGAAGGGGCCCCCGTAATTCCCCCATCCTCTGGGCTCCCA
  CTTCCCACCCCTGATGCCCGGGTAGGGGCCTCCACCTGCGGCCCGCCCGACCACCTGCTG
  CCTCCGCTGGGCACCCCGCTGCCCCCAGACTGGGTGACGCTGGAGGGGGACTTTGTGCTC
  ATGTTGGCCATCTCGCCCAGCCACCTAGGCGCTGACCTGGTGGCAGCTCCGCATGCGCGC
  TTCGACGACGGCCTGGTGCACCTGTGCTGGGTGCGTAGCGGCATCTCGCGGGCTGCGCTG
  CTGCGCCTTTTCTTGGCCATGGAGCGTGGTAGCCACTTCAGCCTGGGCTGTCCGCAGCTG
  GGCTACGCCGCGGCCCGTGCCTTCCGCCTAGAGCCGCTCACACCACGCGGCGTGCTCACA
  GTGGACGGGGAGCAGGTGGAGTATGGGCCGCTACAGGCACAGATGCACCCTGGCATCGGT
  ACACTGCTCACTGGGCCTCCTGGCTGCCCGGGGCGGGAGCCCTGA
  
  Clone variation with respect to NM_020126.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020126 unedited
  TCACATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGGCACCAGCCGGAGACCCGG
  CGGGCCCAGTGTTGGAGAGCTGAAGGTCAGGCCAGGACAGTGAGACCTGACTCCTTGCTC
  CTACCAGCCTACTATGGCTTAAGACCCAGGGCCAGGGTCCCGTTGATGTAACAGAGCAGA
  GGACCAGCAGATGAATGGACACCTTGAAGCAGAGGAGCAGCAGGACCAGAGGCCAGACCA
  GGAGCTGACCGGGAGCTGGGGCCACGGGCCTAGGAGCACCCTGGTCAGGGCTAAGGCCAT
  GGCCCCGCCCCCACCGCCACTGGCTGCCAGCACCCCGCTCCTCCATGGCGAGTTTGGCTC
  CTACCCAGCCCGAGGCCCACGCTTTGCCCTCACCCTTACATCGCAGGCCCTGCACATACA
  GCGGCTGCGCCCCAAACCTGAAGCCAGGCCCCGGGGTGGCCTGGTCCCGTTGGCCGAGGT
  CTCAGGCTGCTGCACCCTGCGAAGCCGCAGCCCCTCAGACTCAGCGGCCTACTTCTGCAT
  CTACACCTACCCTCGGGGCCGGCGCGGGGCCCGGCGCAGAGCCACTCGCACCTTCCGGGC
  AGATGGGGCCGCCACCTACGAAGAGAACCGTGCCGAAGCCCAGCGCTGGGCCACTGCCCT
  CACCTGTCTGCTCCGAGGACTGCCACTGCCCGGGGATGGGGAGATCACCCCTGACCTGCT
  ACCTCGGCCGCCCCGGTTGCTTCTATTGGTCAATCCCTTTGGGGGTCGGGGCCTGGCCTG
  GCAGTGGTGTAAGAAACACGTGCTTCCCATGATCTCTGAAGCTGGGCTGGTCTTCAAACT
  CATCCAGACAGAACGACAGAACCACGCCGGGAGCCTGGTCANGGGCTGAACCTGGATGAG
  TGGATGGCATCGTACGGTCTCGGGAAACGGCTGCTCATGAGTATTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020126 unedited
  AGCTTGTACGCGGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTGAGAGGAATGAA
  GCGACTGCCTTTATTGCATAGACCTTTTCAATTGCTTTTTAGATTGGGGACGGAGGACGC
  GCATGAGACGAACAGGGGATATGAATTTCCCCGCCCCCACCCGCGGGGAGAGGAACATTA
  GTGCAAATCCTAGCGCCGGCCCCGGGGAACCTGCCCCTCCTGGGCTGATTGGCCAGCTAA
  ATGGAGGCACCGGAGTGGATGGGGCGAGGCTGCGGGCCCTGACCGGCCGACTCACTGAGG
  CCTACCCCAGCCAGTACATTCCAGGTCCTGTCATTGGGCGACGCGTAAAATAGACTCCGC
  CCTCAGCCATCCTGGCCGGGTAGGAGCAGGTGGCAAGCGTCAGGACTGAGCCCTGCCCCG
  TAAGGGCGGCCCCAGATCAGACGCCAGGCCCCGCCCTCATTGATCAGGCACTACCCTCGG
  GACGAGCCCATTTCTCTTTAACCGTGACGACGCCCACCTTCCAGCATCACGTCTGGTCCC
  ATGAAAGCGAGCGCAATCCTGAGACGGGGCCAGGGCCCCTGCCACCACAACTCTAGCAGC
  CAGGCCACACCCNCTAGCTCAGGCTCCGCCCCATTGGAATGTANGCCCCGCCCCCCGCGG
  GTACAAGCTTGGTTAGTTTCAAGGCTCCCGCCCCGGGCAGCCAGNAGGCCCAGTGAGCAG
  TGTACCGATGCCAGGGTGCATCTGTGCCTGTAGCGGCCATACTCCAACTGCTCCCCGTCC
  ACTGTGAGCAGCCGCCGTGTGTGAGCGGCTCTAGGCGGAGGGCCAGGGCCGGGGCGTATC
  CCACCTGGCGACAGCCCAGGCTGGAGTGGGCTACCACGCTCCATGGCCAAAAAAAGGCGC
  AGCAACGCAGCCCCGCGAGATGCCGCTACGCACCCAGCCAGGTGCACCAAGCCGTCGTCG
  AACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020126
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020126.3, NP_064511.2
• RefSeq Size: 3012 bp
• RefSeq ORF: 1965 bp
• Locus ID: 56848
• UniProt ID: Q9NRA0
• Cytogenetics: 19q13.33
• Domains: DAGKc
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Fc gamma R-mediated phagocytosis, Metabolic pathways, Sphingolipid metabolism, VEGF signaling pathway
• Gene Summary: This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 3 both encode the same isoform (a).