GALNT1 (NM_020474) Human Untagged Clone

CAT#: SC113088

GALNT1 (untagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) (GALNT1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GALNT1
• Synonyms: GALNAC-T1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113088 sequence for NM_020474 edited (data generated by NextGen Sequencing)
  ATGAGAAAATTTGCATACTGCAAGGTGGTCCTAGCCACCTCCTTGATTTGGGTACTCTTG
  GATATGTTCCTGCTGCTTTACTTCAGTGAATGCAACAAATGTGATGAAAAAAAGGAGAGA
  GGACTTCCTGCTGGAGATGTTCTAGAGCCAGTACAAAAGCCTCATGAAGGTCCTGGAGAA
  ATGGGGAAACCAGTCGTCATTCCTAAAGAGGATCAAGAAAAGATGAAAGAGATGTTTAAA
  ATCAATCAGTTCAATTTAATGGCAAGTGAGATGATTGCACTCAACAGATCTTTACCAGAT
  GTTAGGTTAGAAGGGTGTAAAACAAAGGTGTATCCAGATAATCTTCCTACAACAAGTGTG
  GTGATTGTTTTCCACAATGAGGCTTGGAGCACACTTCTGCGAACTGTCCATAGTGTCATT
  AATCGCTCACCAAGACACATGATAGAAGAAATTGTTCTAGTAGATGATGCCAGTGAAAGA
  GACTTTTTGAAAAGGCCTTTAGAGAGTTATGTGAAAAAACTAAAAGTACCAGTTCATGTA
  ATTCGAATGGAACAACGTTCTGGATTGATCAGAGCTAGATTAAAAGGAGCTGCTGTGTCT
  AAAGGCCAAGTGATCACCTTCCTGGATGCCCATTGTGAGTGTACAGTGGGATGGCTGGAG
  CCTCTCTTGGCCAGGATCAAACATGACAGGAGAACAGTGGTGTGTCCCATCATCGATGTG
  ATCAGTGATGATACTTTTGAGTACATGGCAGGCTCTGATATGACCTATGGTGGGTTCAAC
  TGGAAGCTCAATTTTCGCTGGTATCCTGTTCCCCAAAGAGAAATGGACAGAAGGAAAGGT
  GATCGGACTCTTCCTGTCAGGACACCTACCATGGCAGGAGGCCTTTTTTCAATAGACAGA
  GATTACTTTCAGGAAATTGGAACATATGATGCTGGAATGGATATTTGGGGAGGAGAAAAC
  CTAGAAATTTCCTTTAGGATTTGGCAGTGTGGAGGAACTTTGGAAATTGTTACATGCTCA
  CATGTTGGACATGTGTTTCGGAAAGCTACACCTTACACGTTTCCAGGAGGCACAGGGCAG
  ATTATCAATAAAAATAACAGACGACTTGCAGAAGTGTGGATGGATGAATTCAAGAATTTC
  TTCTATATAATTTCTCCAGGTGTTACAAAGGTAGATTATGGAGATATATCGTCAAGAGTT
  GGTCTAAGACACAAACTACAATGCAAACCTTTTTCCTGGTACCTAGAGAATATATATCCT
  GATTCTCAAATTCCACGTCACTATTTCTCATTGGGAGAGATACGAAATGTGGAAACGAAT
  CAGTGTCTAGATAACATGGCTAGAAAAGAGAATGAAAAAGTTGGAATTTTTAATTGCCAT
  GGTATGGGGGGTAATCAGGTTTTCTCTTATACTGCCAACAAAGAAATTAGAACAGATGAC
  CTTTGCTTGGATGTTTCCAAACTTAATGGCCCAGTTACAATGCTCAAATGCCACCACCTA
  AAAGGCAACCAACTCTGGGAGTATGACCCAGTGAAATTAACCCTGCAGCATGTGAACAGT
  AATCAGTGCCTGGATAAAGCCACAGAAGAGGATAGCCAGGTGCCCAGCATTAGAGACTGC
  AATGGAAGTCGGTCCCAGCAGTGGCTTCTTCGAAACGTCACCCTGCCAGAAATATTCTGA
  
  Clone variation with respect to NM_020474.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020474 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGGGTATGAACGTGA
  TTTCTGATGAAACTGGATTGGAATAATTTTCATGATCTTTGTATATTTATATATATATAT
  TTTTAAATTTTGCATTTGACTTAAAGTGCCATGAGAAAATTTGCATACTGCAAGGTGGTC
  CTAGCCACCTCCTTGATTTGGGTACTCTTGGATATGTTCCTGCTGCTTTACTTCAGTGAA
  TGCAACAAATGTGATGAAAAAAAGGAGAGAGGACTTCCTGCTGGAGATGTTCTAGAGCCA
  GTACAAAAGCCTCATGAAGGTCCTGGAGAAATGGGGAAACCAGTCGTCATTCCTAAAGAG
  GATCAAGAAAAGATGAAAGAGATGTTTAAAATCAATCAGTTCAATTTAATGGCAAGTGAG
  ATGATTGCACTCAACAGATCTTTACCAGATGTTAGGTTAGAAGGGTGTAAAACAAAGGTG
  TATCCAGATAATCTTCCTACAACAAGTGTGGTGATTGTTTTCCACAATGAGGCTTGGAGC
  ACACTTCTGCGAACTGTCCATAGTGTCATTAATCGCTCACCAAGACACATGATAGAAGAA
  ATTGTTCTAGTAGATGATGCCAGTGAAAGAGACTTTNTGAAAAGGCCTTTAGAGAGTTAT
  GTGAAAAACTAANAGTACCAGTTCATGTAATTCGAATGGAACAACGTTCTGGATTGATCA
  GAGCTAGATTAAAGGAGCTGCTGTGTCTAAAGGCAAGTGATCACCTTCCTGGATGCCCAT
  TGTGAGTGTACAGTGGGATGGCTGGAGCCTCTCTTGGNCAGATCAACATGACAGAGAACA
  GNGGTGTGTCCATCATCGATGTGATCAGTGATGATACTTTTGATACATGGCAGCTCTGAT
  ATGACTTATGGTGGGGTCAACTGGAGCTCATTTTGCTTGTTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020474 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTATTCAGGAATATTTTGT
  ATTTTCAAAGTATTTAGGAATTCTTTAATTAGAAAAATAAAGGCAATGGGATTATCTATC
  AGGTTCTAAAAGTACCCATGAATTAAATCACTTACATCTCCCATTTAAAACAAAACCTTA
  AATCACATTGCTGTTAAGCTTTCATACAGTAAGAAATAAGACCAAGCTATGCTGGAGTAC
  AAAAACCTGAAATTCTGAGCATGAAAATAATTTATTGATTAGACATCTTGCTTATTCAAG
  CATTAGGCCTACTGGAGATAGAATTAACACAGCTCATGGAAAAGGAAAAAAATTCCATTC
  AGCACCTGCTTCCTTTCAAATTTCCTGGGGACATGGTTTATGCATTTTGTACTATAGACT
  CACCCTAAAGCATCCCCATGTGCTTGATGTTGATTAACTTGAACAGGATATGAGAAACTT
  TGTGTACACTCAACTTGGCAAATATCATTAGCGATGCGACTTTCGGAAACATTTTTAGGC
  ACAAGAAATACCACTGCCTGCAGTGAAAGCCCTTGATATAAAACAAAAAACCCCATTAAA
  TTCACTTCTCCAATCTGACAGAAAATTTTTGCTTTTTTGCCAACAAACTGTTCTACCTAT
  AAGAAAAAGCGCTAATGATATTCCTCCTCCCAAATAAAAGGTACCTCTTAATGAACACTT
  TACTTGACCCTTAGGAGACTTGTGGGCTCAAACACATCCGAGACACATAACCTGTTTATC
  CTAAATATATTCAGGAAAAAATTCAGCCCCTAATATGGATAACATCCGCCCTTCTTGGTA
  AGCCCTCCAAACCCCCCTCACACTTTTCCCCCTCTCTTTTCCAACACCCGCGAACACTTC
  ACCCTTCCCCCAATCCCAAACTCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020474
• Insert Size: 3780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020474.2, NP_065207.2
• RefSeq Size: 3778 bp
• RefSeq ORF: 1680 bp
• Locus ID: 2589
• UniProt ID: Q10472
• Cytogenetics: 18q12.2
• Domains: RICIN, Glycos_transf_2
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• Gene Summary: This gene encodes a member of the UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family of enzymes. GalNAc-Ts initiate mucin-type O-linked glycosylation in the Golgi apparatus by catalyzing the transfer of GalNAc to serine and threonine residues on target proteins. They are characterized by an N-terminal transmembrane domain, a stem region, a lumenal catalytic domain containing a GT1 motif and Gal/GalNAc transferase motif, and a C-terminal ricin/lectin-like domain. GalNAc-Ts have different, but overlapping, substrate specificities and patterns of expression. Transcript variants derived from this gene that utilize alternative polyA signals have been described in the literature. [provided by RefSeq, Jul 2008]