UGT (UGT2B4) (NM_021139) Human Untagged Clone

CAT#: SC112983

UGT2B4 (untagged)-Human UDP glucuronosyltransferase 2 family, polypeptide B4 (UGT2B4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UGT
• Synonyms: HLUG25; UDPGT2B4; UDPGTh-1; UDPGTH1; UGT2B11
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112983 sequence for NM_021139 edited (data generated by NextGen Sequencing)
  ATGTCTATGAAATGGACTTCAGCTCTTCTGCTGATACAGCTGAGCTGTTACTTTAGCTCT
  GGGAGTTGTGGAAAGGTGCTGGTGTGGCCCACAGAATTCAGCCACTGGATGAATATAAAG
  ACAATCCTGGATGAACTTGTCCAGAGAGGTCATGAGGTGACTGTATTGGCATCTTCAGCT
  TCCATTTCTTTCGATCCCAACAGCCCATCTACTCTTAAATTTGAAGTTTATCCTGTATCT
  TTAACTAAAACTGAGTTTGAGGATATTATCAAGCAGCTGGTTAAGAGATGGGCAGAACTT
  CCAAAAGACACATTTTGGTCATATTTTTCACAAGTACAAGAAATCATGTGGACATTTAAT
  GACATACTTAGAAAGTTCTGTAAGGATATAGTTTCAAATAAGAAACTTATGAAGAAACTA
  CAGGAGTCAAGATTTGATGTTGTTCTTGCAGATGCTGTTTTCCCCTTTGGTGAGCTGCTG
  GCCGAGTTACTTAAAATACCCTTTGTCTACAGCCTCCGCTTCTCTCCTGGCTACGCAATT
  GAAAAGCATAGTGGAGGACTTCTGTTCCCTCCTTCCTATGTGCCTGTTGTTATGTCAGAA
  CTAAGTGACCAAATGACTTTCATAGAGAGGGTAAAAAATATGATCTATGTGCTTTATTTT
  GAATTTTGGTTCCAAATATTTGACATGAAGAAGTGGGATCAGTTCTACAGTGAAGTTCTA
  GGAAGACCCACTACGTTATCTGAGACAATGGCAAAAGCTGACATATGGCTTATTCGAAAC
  TACTGGGATTTTCAATTTCCTCACCCACTCTTACCAAATGTTGAGTTCGTTGGAGGACTC
  CACTGCAAACCTGCCAAACCCCTACCGAAGGAAATGGAAGAGTTTGTCCAGAGCTCTGGA
  GAAAATGGTGTTGTGGTGTTTTCTCTGGGGTCGATGGTCAGTAACACGTCAGAAGAAAGG
  GCCAATGTAATTGCATCAGCCCTTGCCAAGATCCCACAAAAGGTTCTGTGGAGATTTGAT
  GGGAATAAACCAGATACTTTAGGACTCAATACTCGGCTGTACAAGTGGATACCCCAGAAT
  GATCTTCTTGGTCACCCAAAAACCAGAGCTTTTATAACTCATGGTGGAGCCAATGGCATC
  TATGAGGCAATCTACCATGGAATCCCTATGGTGGGCGTTCCATTGTTTGCAGATCAACCT
  GATAACATTGCACACATGAAGGCCAAGGGAGCAGCTGTTAGTTTGGACTTCCACACAATG
  TCGAGTACAGACTTACTCAATGCACTGAAGACAGTAATTAATGATCCTTTATATAAAGAG
  AATGCTATGAAATTATCAAGAATTCATCATGATCAACCAGTGAAGCCCCTTGATCGAGCA
  GTCTTCTGGATTGAATTTGTCATGCGCCATAAAGGAGCCAAGCACCTTCGGGTTGCAGCC
  CACGACCTCACCTGGTTCCAGTACCACTCTTTGGATGTGACTGGGTTCCTGCTGGCCTGT
  GTGGCAACTGTGATATTCATCATCACAAAATGTCTGTTTTGTGTCTGGAAGTTTGTTAGA
  ACAGGAAAGAAGGGGAAAAGAGATTAA
  
  Clone variation with respect to NM_021139.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021139 unedited
  CTTTTGTATACACTCCTATAGGGCGGCCGCGAATTCGCACCAGGNAAAACAAGCATTGCA
  TTGCATCAGGATGTCTATGAAATGGACTTCAGCTCTTCTGCTGATACAGCTGAGCTGTTA
  CTTTAGCTCTGGGAGTTGTGGAAAGGTGCTGGTGTGGCCCACAGAATTCAGCCACTGGAT
  GAATATAAAGACAATCCTGGATGAACTTGTCCAGAGAGGTCATGAGGTGACTGTATTGGC
  ATCTTCAGCTTCCATTTCTTTCGATCCCAACAGCCCATCTACTCTTAAATTTGAAGTTTA
  TCCTGTATCTTTAACTAAAACTGAGTTTGAGGATATTATCAAGCAGCTGGTTAAGAGATG
  GGCAGAACTTCCAAAAGACACATTTTGGTCATATTTTTCACAAGTACAAGAAATCATGTG
  GACATTTAATGACATACTTAGAAAGTTCTGTAAGGATATAGTTTCAAATAAGAAACTTAT
  GAAGAAACTACAGGAGTCAAGATTTGATGTTGTTCTTGCAGATGCTGTTTTCCCCTTTGG
  TGAGCTGCTGGCCGAGTTACTTAAAATACCCTTTGTCTACAGCCTCCGCTTCTCTCCTGG
  CTACGCAATTGAAAAGCATAGTGGAGGACTTCTGTTCCCTCCTTCCTATGTGCCTGTTGN
  TATGTCAGAACTAAGTGACCAAATGACTTTCATAGAGAGGGTAAAAAATATGATCTATGT
  GCTNTATTTTGAATTTTGGTTCCAAATATTTGACATGAAGAAGTGGGATCAGTTCTACAG
  TGAAGTTCTAGGAAGACCCACTACNGTATCTGAGACAATGGCAAAAGCTGACATATGGCC
  TTATTCGAAACTACTGGGGATTTTTCAATTTCCTCACCCACTCTTACCANATGNTTGAGN
  TCGGTTGGAGGAACTCCACTGCAACCTGCCAAACCCCTACGNNAGAAATGGAAGAGTTTG
  TCCGAGCTTGAGAAAAGNGGGTGTNGGGGGTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021139 unedited
  NCACTCTGNNACCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTACA
  ATTTAATTATTTATTAGTTTCCTCAACAACAGTTAAAACAACACAATCCTGCATGAAATG
  GATCCAAAGTATGCTGAGTATTTGCAAAACAGTACTGATACTGTCAGTGGACTTCTTAAT
  GTTCTTGTTTCCATGTACAATGTGTGACATGTAGTTAAGCTTAGTAAATTTTTTTTCATG
  TAACCTGTGAATTGGAACAATAAATTTCAATATAAGCTCAATACATTTCAATATAACCTC
  GTATGGCTTTATATCATTTTTGTTTTCCCTAATGTTTTCCTCCTTGACATGAAATATTTC
  TAATGGTTAAACAGGTACTAAAACAAATTTTGACTTGACAAGGTAAGTTTTGAAAGATGT
  TTTGTCACAAGAAGAAAGGAATCTCTTGTATCACAACGTCTTCTTGTTGTAATAAACTAA
  AGGAGTTCATTTATTGGGTTTCCCAGCTTCCAGCCTCAGACGTAATTAATCTCTTTTCCC
  CTTCTTTCCTGTTCTAACAAACTTCCAGACACAAAACAGACATTNTGTGATGATGAATAT
  CACAGTTGCCACACAGGCCAGCAGGAACCCAGTCACATCCAAAGAGTGGTACTGGAACCA
  GGTGANGTCGTGGGCTGCAACCCGAAGTGCTTGNCTNCTTTTATGCGCATGACANATTCA
  TNCCAGAGACTGCTCGATCAAGGGGCTCACTGGNTGATCATGATGAATTCTTGATAATTN
  CATAGCATNCTCTTTATATAAAGGATCATTAATACTGNNCTCAGNGCATTGAGTAAGTCT
  GTACTCGACATTGNGTGGAAGTTCAAACTACAGCTGCTCCTTTGGCCTCATGNNGTGCAT
  GTATCAAGGTGATCTGCAACCATGGNAACGCCACATANGGATNCATGGTAAAATGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021139
• Insert Size: 2240 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021139.1, NP_066962.1
• RefSeq Size: 2093 bp
• RefSeq ORF: 1587 bp
• Locus ID: 7363
• UniProt ID: P06133
• Cytogenetics: 4q13.3
• Domains: UDPGT
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, Ascorbate and aldarate metabolism, Drug metabolism - cytochrome P450, Drug metabolism - other enzymes, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450, Pentose and glucuronate interconversions, Porphyrin and chlorophyll metabolism, Retinol metabolism, Starch and sucrose metabolism
• Gene Summary: UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme is active on polyhydroxylated estrogens (such as estriol, 4-hydroxyestrone and 2-hydroxyestriol) and xenobiotics (such as 4-methylumbelliferone, 1-naphthol, 4-nitrophenol, 2-aminophenol, 4-hydroxybiphenyl and menthol). It is capable of 6 alpha-hydroxyglucuronidation of hyodeoxycholic acid.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).