ZNF148 (NM_021964) Human Untagged Clone

CAT#: SC112833

ZNF148 (untagged)-Human zinc finger protein 148 (ZNF148)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZNF148
• Synonyms: BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52; ZBP-89; ZFP148
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_021964, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACATTGACGACAAACTGGAAGGATTGTTTCTTAAATGTGGCGGCATAGACGAAATGCAGTCTTCCA
  GGACAATGGTTGTAATGGGTGGAGTGTCTGGCCAGTCTACTGTGTCTGGAGAGCTACAGGATTCAGTACT
  TCAAGATCGAAGTATGCCTCACCAGGAGATCCTTGCTGCAGATGAAGTGTTACAAGAAAGTGAAATGAGA
  CAACAGGATATGATATCACATGATGAACTCATGGTCCATGAGGAGACAGTGAAAAATGATGAAGAGCAGA
  TGGAAACACATGAAAGACTTCCTCAAGGACTACAGTATGCACTTAATGTCCCTATAAGCGTAAAGCAGGA
  AATTACTTTTACTGATGTATCTGAGCAACTGATGAGAGACAAAAAACAAATCAGAGAGCCAGTAGACTTA
  CAGAAAAAGAAGAAGCGGAAACAACGTTCTCCCGCAAAAATCCTTACAATAAATGAGGATGGATCACTTG
  GTTTGAAAACCCCTAAATCTCACGTTTGTGAGCACTGCAATGCTGCCTTTAGAACGAACTATCACTTACA
  GAGACATGTCTTCATTCATACAGGTGAAAAACCATTTCAATGTAGTCAATGTGACATGCGTTTCATACAG
  AAGTACCTGCTTCAGAGACATGAGAAGATTCATACTGGTGAAAAACCATTTCGCTGTGATGAATGTGGTA
  TGAGATTCATACAAAAATATCATATGGAAAGGCATAAGAGAACTCATAGTGGAGAAAAACCTTACCAGTG
  TGAATACTGTTTACAGTATTTTTCCAGAACAGATCGTGTATTGAAACATAAACGTATGTGCCATGAAAAT
  CATGACAAAAAACTAAATAGATGTGCCATCAAAGGTGGCCTTCTGACATCTGAGGAAGATTCTGGCTTTT
  CTACATCACCAAAAGACAACTCACTGCCAAAAAAGAAAAGGCAGAAAACGGAGAAAAAATCATCTGGAAT
  GGACAAAGAGAGTGCTTTGGACAAATCTGACCTGAAAAAAGACAAAAATGATTACTTGCCTCTTTATTCT
  TCAAGTACTAAAGTAAAAGATGAGTATATGGTTGCAGAATATGCTGTTGAAATGCCACATTCGTCAGTTG
  GGGGCTCGCATTTAGAAGATGCGTCAGGAGAAATACACCCACCTAAGTTAGTTCTCAAAAAAATTAATAG
  TAAGAGAAGTCTGAAACAGCCACTGGAGCAAAATCAAACAATTTCACCTTTATCCACATATGAAGAGAGC
  AAAGTTTCAAAGTATGCTTTTGAACTTGTGGATAAACAGGCTTTACTGGACTCAGAAGGCAATGCTGACA
  TTGATCAGGTTGATAATTTGCAGGAGGGGCCCAGTAAACCTGTGCATAGTAGTACTAATTATGATGATGC
  CATGCAGTTTTTGAAGAAGAAGCGGTATCTTCAAGCAGCAAGTAACAACAGCAGGGAATATGCGCTGAAT
  GTGGGTACCATAGCTTCTCAGCCTTCTGTAACACAAGCAGCTGTGGCAAGTGTCATTGATGAAAGTACCA
  CGGCATCCATATTAGAGTCACAGGCACTGAATGTGGAGATTAAGAGTAATCATGACAAAAATGTTATTCC
  AGATGAGGTACTGCAGACTCTGTTGGATCATTATTCCCACAAAGCTAATGGACAGCATGAGATATCCTTC
  AGTGTTGCAGATACTGAAGTGACTTCTAGCATATCAATAAATTCTTCAGAAGTACCAGAGGTCACCCCGT
  CAGAGAATGTTGGATCAAGCTCCCAAGCATCCTCATCAGATAAAGCCAACATGTTGCAGGAATACTCCAA
  GTTTCTGCAGCAGGCTTTGGACAGAACTAGCCAAAATGATGCCTATTTGAATAGCCCGAGCCTTAACTTT
  GTGACTGATAACCAGACCCTCCCAAATCAGCCAGCATTCTCTTCCATAGACAAGCAGGTCTATGCCACCA
  TGCCCATCAATAGCTTTCGATCAGGAATGAATTCTCCACTAAGAACAACTCCAGATAAGTCCCACTTTGG
  ACTAATAGTTGGTGATTCACAGCACTCATTTCCCTTTTCAGGTGATGAGACAAACCATGCTTCTGCCACA
  TCAACACAGGACTTTCTGGATCAAGTGACTTCTCAGAAGAAAGCTGAGGCCCAGCCTGTCCACCAAGCTT
  ACCAAATGAGCTCCTTTGAACAGCCCTTCCGTGCTCCCTATCATGGATCAAGAGCTGGAATAGCTACTCA
  ATTTAGCACTGCCAATGGACAGGTGAACCTTCGGGGACCAGGGACAAGTGCTGAATTTTCAGAATTTCCC
  TTGGTGAATGTAAATGATAATAGAGCTGGGATGACATCTTCACCTGATGCCACAACTGGCCAGACTTTTG
  GCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021964 unedited
  TCAGAAATTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGCCGCCGC
  CTGCAGTCGGTGACCGCGCGACTCGGCGCCCGCCCGCGGATAGAGGGAGGAATCAGCAGC
  TTGGAAATTCAAGCACGTGATCTGGCGGGATGGGCGTTTGCCTAACGTATTTAATGGAGG
  AATCGGATGGCATAAGTGATTAAGGTGGTATTGAGGATTTCTGAAGCCTATGAAAGGTAG
  AAACTCAACCATGATTTCTTTTTCAACTCTACAGCATTCCTTTCCTTGAAGTCTTCGTTT
  TTACCTTAGTCTCGGGCAGTTATACTTAAGCATGAACATTGACGACAAACTGGAAGGATT
  GTTTCTTAAATGTGGCGGCATAGACGAAATGCAGTCTTCCAGGACAATGGTTGTAATGGG
  TGGAGTGTCTGGCCAGTCTACTGTGTCTGGAGAGCTACAGGATTCAGTACTTCAAGATCG
  AAGTATGCCTCACCAGGAGATCCTTGCTGCAGATGAAGTGTTACAAGAAAGTGAAATGAG
  ACAACAGGATATGATATCACATGATGAACTCATGGTCCATGAGGAGACAGTGAAAAATGA
  TGAAGAGCAGATGGAAACACATGAAAGACTTCCTCAAGGACTACAGTATGCACTTAATGT
  CCCTATNAGCGTANAGCAGGAAATTACTTTTACTGATGTATCTGAGCAACTGATGAGAGA
  CANNAAACAATCAGAGAGCCAGTAGACTTACAGAANAAGAAGAAAGCGGAAACACGTTCT
  CCCCGCAAAATCCTTTACATAATGAGGATGGATCACTTGGTTNGAAACCCCCTAATCTCA
  CGTTTGTGAGCACTTGCATGCTGNCTTTAGAAACGACTATCACTACAGAGACATGTCTTC
  ATTCATACAGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021964 unedited
  GGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAGCCAAAAGTCTG
  GCCAGTTGTGGCATCAGGTGAAGATGTCATCCCAGCTCTATTATCATTTACATTCACCAA
  GGGAAATTCTGAAAATTCAGCACTTGTCCCTGGTCCCCGAAGGTTCACCTGTCCATTGGC
  AGTGCTAAATTGAGTAGCTATTCCAGCTCTTGATCCATGATAGGGAGCACGGAAGGGCTG
  TTCAAAGGAGCTCATTTGGTAAGCTTGGTGGACAGGCTGGGCCTCAGCTTTCTTCTGAGA
  AGTCACTTGATCCAGAAAGTCCTGTGTTGATGTGGCAGAAGCATGGTTTGTCTCATCACC
  TGAAAAGGGAAATGAGTGCTGTGAATCACCAACTATTAGTCCAAAGTGGGACTTATCTGG
  AGTTGTTCTTAGTGGAGAATTCATTCCTGATCGAAAGCTATTGATGGGCATGGTGGCATA
  GACCTGCTTGTCTATGGAAGAGAATGCTGGCTGATTTGGGAGGGTCTGGTTATCAGTCAC
  AAAGTTAAGGCTCGGGCTATTCAAATAGGCATCATTTTGGCTAGTTCTGTCCAAAGCCTG
  CTGCAGAAACTTGGAGTATTCCTGCAACATGTTGGCTTTATCTGATGAGGATGCTTGNGA
  GCTTGATCCAACATTCTCTGATGGGGTGACCTCTGGTACTTCTGAAGAATTTATTGATAT
  GCTAGAAGTCACTTCAGTATCTGCAACACTGAAGGATATCTCATGCTGTCCATTAGCTNT
  GTGGGAATAATGATCCAACAGAGTCTGCAGTACCTCATCTGGAAATACATTNTGNCATGA
  ATACCTCTAATCTCCACATTCAGTGCCTGTGACTCTATATGGATGCCGTGGTACTTTCAT
  CATGACACTGCACAGCTGCTTGTGTACAGAAGCTGAAAGCTATGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021964
• Insert Size: 3090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021964.1, NP_068799.1
• RefSeq Size: 3032 bp
• RefSeq ORF: 2385 bp
• Locus ID: 7707
• UniProt ID: Q9UQR1
• Cytogenetics: 3q21.2
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: The protein encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. The encoded protein activates transcription of the T-cell receptor and intestinal alkaline phosphatase genes but represses transcription of the ornithine decarboxylase, vimentin, gastrin, stomelysin, and enolase genes. Increased expression of this gene results in decreased patient survival rates from colorectal cancer, while mutations in this gene have been associated with global developmental delay, hypoplastic corpus callosum, and dysmorphic facies. [provided by RefSeq, Feb 2017]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-11 all encode the same isoform (a).