GALNT11 (NM_022087) Human Untagged Clone

CAT#: SC112822

GALNT11 (untagged)-Human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 11 (GalNAc-T11) (GALNT11)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GALNT11
• Synonyms: GALNAC-T11; GALNACT11
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112822 sequence for NM_022087 edited (data generated by NextGen Sequencing)
  ATGGGAAGTGTCACAGTTCGGTATTTCTGTTATGGGTGCCTTTTTACATCTGCGACCTGG
  ACAGTTTTGCTTTTTGTTTATTTCAACTTCAGTGAAGTGACTCAGCCACTTAAGAATGTG
  CCCGTCAAGGGGTCTGGGCCCCACGGACCATCTCCAAAAAAATTCTATCCCCGTTTCACT
  CGAGGCCCAAGTCGAGTGCTCGAGCCACAGTTCAAAGCAAACAAAATTGACGATGTGATA
  GACAGTCGTGTTGAAGATCCAGAAGAAGGCCACTTGAAATTCTCTTCTGAATTAGGTATG
  ATTTTTAATGAACGCGATCAAGAGTTGAGAGACTTGGGCTATCAGAAACATGCTTTTAAT
  ATGCTTATCAGTGACCGCTTGGGCTACCACAGAGATGTGCCAGACACAAGGAATGCAGCA
  TGTAAAGAAAAGTTCTACCCACCTGACCTGCCAGCTGCTAGTGTTGTTATCTGTTTCTAT
  AATGAAGCGTTTTCTGCCTTGCTTCGGACAGTGCACAGTGTCATAGACCGCACGCCAGCA
  CACCTGCTTCATGAGATCATCCTTGTGGATGATGATAGTGACTTTGATGATTTGAAAGGA
  GAACTAGATGAATATGTCCAAAAATACCTCCCTGGAAAAATTAAAGTCATAAGAAATACA
  AAGCGTGAGGGGTTGATTCGAGGGAGAATGATTGGCGCGGCCCACGCGACAGGAGAAGTC
  CTTGTGTTCCTGGACAGCCACTGTGAAGTGAATGTGATGTGGCTGCAGCCCTTGCTGGCC
  GCCATCCGTGAGGACCGGCACACCGTGGTGTGCCCAGTGATTGACATCATCAGCGCCGAC
  ACGCTGGCCTACAGCTCGTCCCCTGTCGTCCGCGGAGGGTTCAACTGGGGACTGCACTTC
  AAATGGGATCTTGTCCCCCTTTCTGAGCTAGGACGAGCGGAGGGAGCCACTGCACCAATA
  AAGTCACCAACAATGGCTGGAGGTTTGTTTGCCATGAACAGACAGTATTTCCATGAACTT
  GGACAGTATGATAGTGGCATGGATATCTGGGGAGGAGAAAATTTGGAAATATCATTTCGG
  ATCTGGATGTGTGGCGGTAAGCTCTTCATCATCCCTTGCTCTAGAGTAGGACACATTTTC
  CGAAAAAGGCGACCATATGGATCTCCCGAAGGCCAGGACACCATGACACACAACTCTTTG
  CGGCTGGCACATGTCTGGTTGGATGAATACAAGGAGCAGTATTTTTCCTTAAGACCTGAC
  CTGAAGACGAAAAGCTATGGCAATATCAGTGAGCGTGTGGAACTGAGAAAGAAGTTGGGC
  TGTAAATCATTTAAATGGTATTTGGATAATGTATACCCAGAGATGCAGATATCTGGGTCC
  CACGCCAAACCCCAACAACCCATTTTTGTCAATAGAGGGCCAAAACGACCCAAAGTCCTT
  CAACGTGGAAGGCTCTATCACCTCCAGACCAACAAATGCCTGGTGGCCCAGGGCCGCCCA
  AGTCAGAAGGGAGGTCTCGTGGTGCTTAAGGCCTGTGACTACAGTGACCCAAATCAGATC
  TGGATCTATAATGAAGAGCATGAATTGGTTTTAAATAGTCTCCTTTGTCTAGATATGTCA
  GAGACTCGCTCATCAGACCCGCCACGGCTCATGAAATGCCACGGGTCAGGAGGATCCCAG
  CAGTGGACCTTTGGGAAAAACAATCGGCTATACCAGGTGTCGGTTGGACAGTGCCTGAGA
  GCAGTGGATCCCCTGGGTCAGAAGGGCTCTGTCGCCATGGCGATCTGCGATGGCTCCTCT
  TCACAGCAGTGGCATTTGGAAGGTTAA
  
  Clone variation with respect to NM_022087.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022087 unedited
  GTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGTACTATCCATACATCTTC
  TTGTATTTGAATATCCGTTAACTGTACGCATAGTGGTCCTACTTGGTGGTTTGGAAAACA
  GTGATTCTGTATTTGGTGGATGGTTTAAAGTATACTGAAGTGTCTAACATATTTATTCTT
  CTTTTTTAGGAAATTGACAATGGCCCTTCAGCTATGCTAGGTCTATAATGGGAAGTGTCA
  CAGTTCGGTATTTCTGTTATGGGTGCCTTTTTACATCTGCGACCTGGACAGTTTTGCTTT
  TTGTTTATTTCAACTTCAGTGAAGTGACTCAGCCACTTAAGAATGTGCCCGTCAAGGGGT
  CTGGGCCCCACGGACCATCTCCAAAAAAATTCTATCCCCGTTTCACTCGAGGCCCAAGTC
  GAGTGCTCGAGCCACAGTTCAAAGCAAACAAAATTGACGATGTGATAGACAGTCGTGTTG
  AAGATCCAGAAGAAGGCCACTTGAAATTCTCTTCTGAATTAGGTATGATTTTTAATGAAC
  GCGATCAAGAGTTGAGAGACTTGGGCTATCAGAAACATGCTTTTAATATGCTTATCAGTG
  ACCGCTTGNGCTACCACAGAGATGTGCCAGACACAAGGAATGCAGCATGTAAAGAAAAGT
  TCTACCCACCTGACCTGCCAGCTGCTAGNNNGTGTATCTGTTTCTATAATGAAGCGTTTT
  CTGCCTTGCTTCGGACAGTGCACAGTGTCATAGACCGCACCCAGCACACCTGCTNATGAG
  ATCATNCTTGGGATGATGATAGTGACTTTGATGATTGGAAAGGAGAACTAGATGATATGT
  CAAAATACCTCCCTGAANAATNAAGTCATAGAAATACAAGCGTGAGGGGTTGATTCNAGG
  AGATGATTGGCGCCGCCCACCAAAGGAAAATTCTGTGTCCTGNACGCCACTGGAGTGATG
  GAGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022087 unedited
  GACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATCAAAGGATTCTTTA
  TTATGTAGATTGTATCATAATTCATCAAAATAATTTTATAATTAACTGCCATACTGTCTT
  ATAAATGATGCCTTCTGAAGTTAAACATAATATAGATTCAGTTTTTTAAAATGATCTACT
  TACAACATACTAAACAAATAATAGAAGTTTTTTTTTTTTTTAAAGCACTATCTCCTATGA
  AGTCTAAGAAAGGAAATCATGACAAGTTCCTGATCAGATAAAAACCACTGCATCATTAAA
  GGGGATTAAAAAGAGGCATAAATAAATCTGAATAGAAATATCCATTTTCCTGGGTTTTAT
  CCTTCTGTACAAGCCCAATTCTAGAGTTAAAATGATACTGTGGTTTGCCTCTATAAGCAA
  GAAATTCCTTAACAGCAGCTCAAACATCACCTTAATGTCCAGGTGAAACATACTCACTTC
  TTCACCCGTGGACACTCACTTCTTCACCCGTGGACACTCAAAGCTCCCGTCTCTGTCAGA
  CACGTGTCACCTCTGCTAACACACCCAGGCATCTCCGGGAGGACAGGGAAACTGTCATTT
  TTCTCACAAATGGTTTCTGCTCCACCCAACCCCAGCTTTCCTTAAAGCCCCAACCTCCAC
  ACCGGGAGGGACCCCCTGATGAAACACCGTTCCCCGCCAAGATTTCACTTTACCTTCCCA
  AGGCCACTGTTGGGAAAAGAAGCCATTTATATCGCCATGGGAACAAACCCCTCCTGCACA
  CTGGAACACGGCTTTAAGCACTGTCACCGCACCTTGTTTACCATTTGTTTTCACAAAGGC
  CACTGGTGGGACCCCCAGACCCTGGCTTCATTACCCCTTGCCATCTCAACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022087
• Insert Size: 2940 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022087.1, NP_071370.1
• RefSeq Size: 2591 bp
• RefSeq ORF: 1827 bp
• Locus ID: 63917
• UniProt ID: Q8NCW6
• Cytogenetics: 7q36.1
• Domains: RICIN, Glycos_transf_2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, O-Glycan biosynthesis
• Gene Summary: Polypeptide N-acetylgalactosaminyltransferase that catalyzes the initiation of protein O-linked glycosylation and is involved in left/right asymmetry by mediating O-glycosylation of NOTCH1. O-glycosylation of NOTCH1 promotes activation of NOTCH1, modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). Polypeptide N-acetylgalactosaminyltransferases catalyze the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor. Displays the same enzyme activity toward MUC1, MUC4, and EA2 than GALNT1. Not involved in glycosylation of erythropoietin (EPO).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).