PSMB9 (NM_002800) Human Untagged Clone

CAT#: SC111676

PSMB9 (untagged)-Human proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2) (PSMB9)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMB9
• Synonyms: beta1i; LMP2; PRAAS3; PSMB6i; RING12
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111676 sequence for NM_002800 edited (data generated by NextGen Sequencing)
  ATGCTGCGGGCGGGAGCACCAACCGGGGACTTACCCCGGGCGGGAGAAGTCCACACCGGG
  ACCACCATCATGGCAGTGGAGTTTGACGGGGGCGTTGTGATGGGTTCTGATTCCCGAGTG
  TCTGCAGGCGAGGCGGTGGTGAACCGAGTGTTTGACAAGCTGTCCCCGCTGCACGAGCAC
  ATCTACTGTGCACTCTCTGGTTCAGCTGCTGATGCCCAAGCCGTGGCCGACATGGCCGCC
  TACCAGCTGGAGCTCCATGGGATAGAACTGGAGGAACCTCCACTTGTTTTGGCTGCTGCA
  AATGTGGTGAGAAATATCAGCTATAAATATCGAGAGGACTTGTCTGCACATCTCATGGTA
  GCTGGCTGGGACCAACGTGAAGGAGGTCAGGTATATGGAACCCTGGGAGGAATGCTGACT
  CGACAGCCTTTTGCCATTGGTGGCTCCGGCAGCACCTTTATCTATGGTTATGTGGATGCA
  GCATATAAGCCAGGCATGTCTCCCGAGGAGTGCAGGCGCTTCACCACAGACGCTATTGCT
  CTGGCCATGAGCCGGGATGGCTCAAGCGGGGGTGTCATCTACCTGGTCACTATTACAGCT
  GCCGGTGTGGACCATCGAGTCATCTTGGGCAATGAACTGCCAAAATTCTATGATGAGTGA
  
  Clone variation with respect to NM_002800.4
  179 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002800 unedited
  NGTTCAGATTTTNGTATACGACTCACTATAGGGCGGCCGCGAAATTCGCACGAGGGGAGA
  GCGGTGCCTTGCAGGGATGCTGCGGGCGGGAGCACCAACCGGGGACTTACCCCGGGCGGG
  AGAAGTCCACACCGGGACCACCATCATGGCAGTGGAGTTTGACGGGGGCGTTGTGATGGG
  TTCTGATTCCCGAGTGTCTGCAGGCGAGGCGGTGGTGAACCGAGTGTTTGACAAGCTGTC
  CCCGCTGCACGAGCACATCTACTGTGCACTCTCTGGTTCAGCTGCTGATGCCCAAGCCGT
  GGCCGACATGGCCGCCTACCAGCTGGAGCTCCATGGGATAGAACTGGAGGAACCTCCACT
  TGTTTTGGCTGCTGCAAATGTGGTGAGAAATATCAGCTATAAATATCGAGAGGACTTGTC
  TGCACATCTCATGGTAGCTGGCTGGGACCAACGTGAAGGAGGTCAGGTATATGGAACCCT
  GGGAGGAATGCTGACTCGACAGCCTTTTGCCATTGGTGGCTCCGGCAGCACCTTTATCTA
  TGGTTATGTGGATGCAGCATATAAGCCAGGCATGTCTCCCGAGGAGTGCAGGCGCTTCAC
  CACAGACGCTATTGCTCTGGCCATGAGCCGGGATGGCTCAAGCGGNGGTGTCATCTACCT
  GGTCACTATTACAGCTGCCGGTGTGGACCATCGAGTCATCTTGGGCAATGAACTGCCAAA
  TTCTATGATGAGTGAACCTTCCCCAGACTTCTCTTTCTTATTTTGTAATANACTCTCTTA
  GGCCAAAAANNAAAANNNNNNNNNNNANNANNNANNATNNAAATTANATTANNAAAAAAT
  TAAATAAATATTTTAAAAAAAAAAAAAAACCTTCCTTTTATATGGGCCCGGGTTTTATTT
  TTTTTGAAAAAACCCGGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002800 unedited
  GAGAGATTTTTACAAATAANAAAGAAATCTGGGNGAGGTTCACTCATCATAGAATTTTGG
  CAGTTCATTGCCCAAGATGACTCGATGGTCCACACCGGCAGCTGTAATAGTGACCAGGTA
  GATGACACCCCCGCTTGAGCCATCCCGGCTCATGGCCAGAGCAATAGCGTCTGTGGTGAA
  GCGCCTGCACTCCTCGGGAGACATGCCTGGCTTATATGCTGCATCCACATAACCATAGAT
  AAAGGTGCTGCCGGAGCCACCAATGGCAAAAGGCTGTCGAGTCAGCATTCCTCCCAGGGT
  TCCATATACCTGACCTCCTTCACGTTGGTCCCAGCCAGCTACCATGAGATGTGCAGACAA
  GTCCTCTCGATATTTATAGCTGATATTTCTCACCACATTTGCAGCAGCCAAAACAAGTGG
  AGGTTCCTCCAGTTCTATCCCATGGAGCTCCAGCTGGTAGGCGGCCATGTCGGCCACGGC
  TTGGGCATCAGCAGCTGAACCAGAGAGTGCACAGTAGATGTGCTCGTGCAGCGGGGACAG
  CTTGTCAAACACTCGGTTCACCACCGCCTCGCCTGCAGACACTCGGGAATCAGAACCCAT
  CACAACGCCCCCGTCAAACTCCACTGCCATGATGGTGGTCCCGGTGTGGACTTCTCCCGC
  CCGGNGTAAGTCCCCGGTTGGTGCTCCCGCCCGCAGCATCCCTGCAAGGCACCGCTCTCC
  CCTCGTGCCGAATTCGCGGCCGNCCTATAGTGAGTCGTATTACAAAAATCTGACGGGTCA
  CTAAACGAGCTCTGCTTATATAGACCTNCCACCGTACACGCCTACCGNCCATTTGCGTCA
  ACGGGGCGGGGNTATACGANCATTTGGAAGTCCNGTTGATTNTGGTGCCCAAACAAACTN
  CCATGACGTCATGGGGTGGAGACTTGAAAATCCCGGAGTCAACCGCTATCCGCCCATGGT
  GACTGGCAAACCGATACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002800
• Insert Size: 780 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002800.4, NP_002791.1
• RefSeq Size: 1048 bp
• RefSeq ORF: 660 bp
• Locus ID: 5698
• UniProt ID: P28065
• Cytogenetics: 6p21.32
• Domains: proteasome
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• Gene Summary: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq, Mar 2010]
  Transcript Variant: This variant (1) encodes the longer isoform (1).