PRELP (NM_002725) Human Untagged Clone

CAT#: SC111673

PRELP (untagged)-Human proline/arginine-rich end leucine-rich repeat protein (PRELP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRELP
• Synonyms: MST161; MSTP161; SLRR2A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111673 sequence for NM_002725 edited (data generated by NextGen Sequencing)
  ATGAGGTCACCCCTCTGCTGGCTCCTCCCACTTCTCATCTTGGCCTCAGTGGCCCAAGGC
  CAGCCAACAAGACGACCAAGACCCGGGACTGGGCCCGGGCGCAGACCCAGGCCCAGGCCC
  AGGCCCACACCCAGCTTTCCTCAGCCTGATGAACCAGCAGAGCCAACAGACCTGCCTCCT
  CCCCTCCCTCCAGGCCCTCCATCTATCTTCCCTGACTGTCCCCGCGAATGCTACTGCCCC
  CCTGATTTCCCATCTGCCCTCTACTGTGATAGCCGCAACCTGCGAAAGGTCCCTGTCATC
  CCGCCCCGCATCCATTACCTCTATCTCCAGAACAACTTCATCACTGAGCTCCCGGTGGAG
  TCCTTCCAGAATGCCACAGGCCTGCGATGGATTAACCTGGACAACAACCGAATCCGCAAG
  ATAGACCAGAGGGTGCTGGAGAAACTGCCCGGCCTGGTGTTCCTCTACATGGAGAAGAAC
  CAGTTGGAAGAGGTCCCCTCGGCCCTGCCCCGGAACCTGGAGCAGCTGAGGCTGAGCCAG
  AACCACATCTCCAGAATCCCGCCTGGTGTCTTCAGCAAGCTGGAGAACCTGCTGCTCCTG
  GATCTCCAGCACAACAGGCTGAGCGACGGCGTCTTCAAGCCCGACACCTTCCATGGCCTC
  AAGAACCTCATGCAGCTCAACCTGGCCCACAACATCCTGAGAAAGATGCCGCCCAGGGTC
  CCCACCGCCATTCACCAGCTCTACCTGGACAGTAACAAGATTGAGACCATCCCTAACGGA
  TACTTCAAGAGCTTTCCCAATCTTGCCTTCATTCGGCTTAACTACAACAAGCTGACAGAC
  AGGGGACTCCCCAAGAACTCCTTTAATATCTCCAACCTGCTTGTGCTCCACCTGTCCCAC
  AACAGGATCAGCAGTGTGCCCGCCATCAACAACAGGCTGGAACACCTGTACCTCAACAAC
  AATAGCATCGAGAAAATCAACGGAACCCAGATTTGCCCCAACGACCTAGTGGCGTTCCAT
  GACTTCTCCTCGGACCTGGAGAACGTGCCACACCTGCGCTACCTGCGGCTGGATGGAAAC
  TACTTGAAGCCGCCCATCCCGCTGGACCTCATGATGTGCTTCCGCCTCCTGCAGTCCGTG
  GTCATCTAG
  
  Clone variation with respect to NM_002725.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002725 unedited
  TTGGATTTTGTAATACGACTCACTATAGGGCGGCCGCGNAATTCGCACCANATCTTCTAG
  CTGGCTCTCTGCTGCCACAGCTCCGCCGAAGGGAGGGGGTGGAAGAGGAGGACTAAACTC
  AGAGCTGAGAGGAGAGGCAGGTGTGTGCAGGTGCATCACCTGGATCATGAGGTCACCCCT
  CTGCTGGCTCCTCCCACTTCTCATCTTGGCCTCAGTGGCCCAAGGCCAGCCAACAAGACG
  ACCAAGACCCGGGACTGGGCCCGGGCGCAGACCCAGGCCCAGGCCCAGGCCCACACCCAG
  CTTTCCTCAGCCTGATGAACCAGCAGAGCCAACAGACCTGCCTCCTCCCCTCCCTCCAGG
  CCCTCCATCTATCTTCCCTGACTGTCCCCGCGAATGCTACTGCCCCCCTGATTTCCCATC
  TGCCCTCTACTGTGATAGCCGCAACCTGCGAAAGGTCCCTGTCATCCCGCCCCGCATCCA
  TTACCTCTATCTCCAGAACAACTTCATCACTGAGCTCCCGGTGGAGTCCTTCCAGAATGC
  CACAGGCCTGCGATGGATTAACCTGGACAACAACCGAATCCGCAAGATAGACCAGAGGGT
  GCTGGAGAAACTGCCCGGCCTGGTGTTCCTCTACATGGAGAAGAACCAGTTGGAAGAGGT
  CCCCTCGGCCCTGCCCCGGAACCTGGAGCAGCTGAGGCTGAGCCAGAACCACATCTCCAG
  AATCCCGCCTGGTGTCTTCAGCAAGCTGGAGAACCTGCTGCTCCTGGATCTCCAGCACAA
  CAGGCTGAGCGACGGCGTCTTCAAGCCCCGACACCTTCATGGGCCTCAGAACTCATGCAG
  CTCAACCTGGCCCACACATCCTGAGAAGATGCCGCCAGGGTCCCCACGNCATTCACAGCT
  CTACCTGGCAGTNACAGAATGAGACATCCCTACCGATACTCAGAGCTTTCCAATCTGCCT
  NNCATCGCTAACTACACCAGCTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002725 unedited
  GGCTCTATTAGNACCGCGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTTTTTTCT
  TTAAACACATCTGTGTTTCTGGAGCAAAGGGAAACCACAGAAGGCCAGGAGTTTGGGTGT
  GCACTGGGTGTCTTTCAACTGGGTGGAACCAAACTGAGTCCTTGAAGTCTCGCTCCTGAG
  GCTGCAGAAGAATAGATGGCTTTTCCCTGCCTCGAGGGTGGGATAAGGGAGGCAAAGCTG
  GGAGAAAGAAAGGGAGAGAGAGAAAACGAATGGCCGGCACAGGTGCCTGGGCCCCAGCCT
  TCAAGTGCGGTCAGAGCAGATCCGGTGGCGGAGTAGGGCCTATATGACCACGGACTGCAG
  GAGGCGGAAGCACATCATGAGGTCCAGCGGGATGGGCGGCTTCAAGTAGTTTCCATCCAG
  CCGCAGGTAGCGCAGGTGTGGCACGTTCTCCAGGTCCGAGGAGAAGTCATGGAACGCCAC
  TAGGTCGTTGGGGCAAATCTGGGTTCCGTTGATTTTCTCGATGCTATTGTTGTTGAGGTA
  CAGGTGTTCCAGCCTGTTGTTGATGGCGGGCACACTGCTGATCCTGTTGTGGGACAGGTG
  GAGCACAAGCAGGTTGGAGATATTAAAGGAGTTCTTGGGGAGTCCCCTGTCTGTCAGCTT
  GTTGTAGTTAAGCCGAATGAAGGCAAGATTGGGAAAGCTCTTGAAGTATCCGTTAGGGAT
  GGTCTCAATCTTGTTACTGTCCAGGTAGAGCTGGTGAATGGGCGTGGGGACCCTGGGCGG
  CATCTTTCTCAAGATGTTGTGGGCCAAGNTGACCTGCATGAAGTTCTTTGAGCCATGGAA
  GGTGTCGGGCTTGAAAACCCCGTCGCTCAACCTGTTGTGCTGAAA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002725
• Insert Size: 1850 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002725.3, NP_002716.1
• RefSeq Size: 5833 bp
• RefSeq ORF: 1149 bp
• Locus ID: 5549
• UniProt ID: P51888
• Cytogenetics: 1q32.1
• Domains: LRRNT, LRR, LRR_TYP, LRR_PS
• Protein Families: Secreted Protein
• Gene Summary: The protein encoded by this gene is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the same protein as variant 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.