Cannabinoid Receptor I (CNR1) (NM_016083) Human Untagged Clone

CAT#: SC111611

CNR1 (untagged)-Human cannabinoid receptor 1 (brain) (CNR1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cannabinoid Receptor I
• Synonyms: CANN6; CB-R; CB1; CB1A; CB1K5; CB1R; CNR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111611 sequence for NM_016083 edited (data generated by NextGen Sequencing)
  ATGAAGTCGATCCTAGATGGCCTTGCAGATACCACCTTCCGCACCATCACCACTGACCTC
  CTGTACGTGGGCTCAAATGACATTCAGTACGAAGACATCAAAGGTGACATGGCATCCAAA
  TTAGGGTACTTCCCACAGAAATTCCCTTTAACTTCCTTTAGGGGAAGTCCCTTCCAAGAG
  AAGATGACTGCGGGAGACAACCCCCAGCTAGTCCCAGCAGACCAGGTGAACATTACAGAA
  TTTTACAACAAGTCTCTCTCGTCCTTCAAGGAGAATGAGGAGAACATCCAGTGTGGGGAG
  AACTTCATGGACATAGAGTGTTTCATGGTCCTGAACCCCAGCCAGCAGCTGGCCATTGCA
  GTCCTGTCCCTCACGCTGGGCACCTTCACGGTCCTGGAGAACCTCCTGGTGCTGTGCGTC
  ATCCTCCACTCCCGCAGCCTCCGCTGCAGGCCTTCCTACCACTTCATCGGCAGCCTGGCG
  GTGGCAGACCTCCTGGGGAGTGTCATTTTTGTCTACAGCTTCATTGACTTCCACGTGTTC
  CACCGCAAAGATAGCCGCAACGTGTTTCTGTTCAAACTGGGTGGGGTCACGGCCTCCTTC
  ACTGCCTCCGTGGGCAGCCTGTTCCTCACAGCCATCGACAGGTACATATCCATTCACAGG
  CCCCTGGCCTATAAGAGGATTGTCACCAGGCCCAAGGCCGTGGTGGCGTTTTGCCTGATG
  TGGACCATAGCCATTGTGATCGCCGTGCTGCCTCTCCTGGGCTGGAACTGCGAGAAACTG
  CAATCTGTTTGCTCAGACATTTTCCCACACATTGATGAAACCTACCTGATGTTCTGGATC
  GGGGTCACCAGCGTACTGCTTCTGTTCATCGTGTATGCGTACATGTATATTCTCTGGAAG
  GCTCACAGCCACGCCGTCCGCATGATTCAGCGTGGCACCCAGAAGAGCATCATCATCCAC
  ACGTCTGAGGATGGGAAGGTACAGGTGACCCGGCCAGACCAAGCCCGCATGGACATTAGG
  TTAGCCAAGACCCTGGTCCTGATCCTGGTGGTGTTGATCATCTGCTGGGGCCCTCTGCTT
  GCAATCATGGTGTATGATGTCTTTGGGAAGATGAACAAGCTCATTAAGACGGTGTTTGCA
  TTCTGCAGTATGCTCTGCCTGCTGAACTCCACCGTGAACCCCATCATCTATGCTCTGAGG
  AGTAAGGACCTGCGACACGCTTTCCGGAGCATGTTTCCCTCTTGTGAAGGCACTGCGCAG
  CCTCTGGATAACAGCATGGGGGACTCGGACTGCCTGCACAAACACGCAAACAATGCAGCC
  AGTGTTCACAGGGCCGCAGAAAGCTGCATCAAGAGCACGGTCAAGATTGCCAAGGTAACC
  ATGTCTGTGTCCACAGACACGTCTGCCGAGGCTCTGTGA
  
  Clone variation with respect to NM_016083.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016083 unedited
  ACTCACTATAGGGCGGCCCGCGATTCGGCACGAGGGCCTAATCAAAGACTGAGGTTATGA
  AGTCGATCCTAGATGGCCTTGCAGATACCACCTTCCGCACCATCACCACTGACCTCCTGT
  ACGTGGGCTCAAATGACATTCAGTACGAAGACATCAAAGGTGACATGGCATCCAAATTAG
  GGTACTTCCCACAGAAATTCCCTTTAACTTCCTTTAGGGGAAGTCCCTTCCAAGAGAAGA
  TGACTGCGGGAGACAACCCCCAGCTAGTCCCAGCAGACCAGGTGAACATTACAGAATTTT
  ACAACAAGTCTCTCTCGTCCTTCAAGGAGAATGAGGAGAACATCCAGTGTGGGGAGAACT
  TCATGGACATAGAGTGTTTCATGGTCCTGAACCCCAGCCAGCAGCTGGCCATTGCAGTCC
  TGTCCCTCACGCTGGGCACCTTCACGGTCCTGGAGAACCTCCTGGAGCTGTGCGTCATCC
  TCCACTCCCGCAGCCTCCGCTGCAGGCCTTCCTACCACTTCATCGGCAGCCTGGCGGTGG
  CAGACCTCCTGGGGAGTGTCATTTTTGTCTACAGCTTCATTGACTTCCACGTGTTCCACC
  GCATAGATAGCCGCAACGTGTTTCTGTTCAAACTGGGTGGGGTCACGGNCTCCTTCACTG
  CCTACGTGGGCAGCCTGTTCCTCACAGCCATCGACAGGTACATATCCATTCACAGGCCCC
  TGGCCTATAAGAGGATTGTCACCAGGCCCAAGGCCGCTGGTGGCGTTTTGCCTGATGTGC
  ACCATAGCCATTGTGATCGCCGTGCTGCCTCTTCTGGGCTGGGACTGCNAGAACTGCAAT
  CTGGTTGCTCAGACATTTCCCACAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016083 unedited
  NNNGGGTATACTATGNNACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTTTTT
  TTGTTCTTCATGGTAATCTAGAATTTTTATTTGATTCCAGGTAACTTTGGACAATAGACA
  TGATGAGATGTTTTCTCTTTGAATAAGTTAACAGTTTAATTACATTGAAATAAAATATAC
  TGTGGGCTTAATACATTGTAAATATTACAGAAGAAGTACTACTTTGCTATAGTAATTTAA
  TGACTGAAGCTACATTTAATAACAATCTTACCAGTACTTGTATACAAAGTATTATACAGA
  TTACTAACGAAGATATTGCAGTGGTTGCAACGATGTTACCAGCTCAACAAACATCTAACA
  ATACAATATTCTTCTAAGAGGAAAAGTAATAATGTTAGATTTACAGACAATAAAGAGTCA
  TGTTTCCCGCTTGAAACATTGGAAATAAATTTGAATCCAGATACAAGAAAAGGTTTCCTT
  TCTTGGGAACTCTGATCCCCAGTAGGCCTAGTACAGTCAATTTACATGAAGATAGCTTTT
  AAGATGCCACGGCAATGTAAAGAAACTCTCCCATCCGAAAATTACTGATTTGTAGGCCAC
  CTGCTCAAACATCTGACTTTTTAGAAACCTATTTACATACATTCAGCCCAAATCAATAGA
  AAGAATGTTTTTTTCTATATTACCATAGGCACTTGTAACCTTTCCACCAAAGGGCCGTGC
  GAGACAATCAATGTTAAAAGCCTTGGCCCCCCTTTAGGAAAATTTAAAGCCCCACCAATT
  TTCACCTCGAGTGCGGCAATCCCACTTTTGGCTGATTCGGCGGGACCCTTAGACAGCCGC
  GCACGGCCACCCATTAACCACCGGACCGCATCACATCGCTCAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016083
• Insert Size: 5700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016083.3, NP_057167.2
• RefSeq Size: 5486 bp
• RefSeq ORF: 1419 bp
• Locus ID: 1268
• UniProt ID: P21554
• Cytogenetics: 6q15
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes one of two cannabinoid receptors. The cannabinoids, principally delta-9-tetrahydrocannabinol and synthetic analogs, are psychoactive ingredients of marijuana. The cannabinoid receptors are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family, which inhibit adenylate cyclase activity in a dose-dependent, stereoselective and pertussis toxin-sensitive manner. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. Multiple transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, May 2009]
  Transcript Variant: This variant (1) encodes the longer isoform (a). Variant 1 has also been called CB1A by PubMed ID: 15289816. Variants 1, 3, 4 and 5 all encode isoform a.