TPK1 (NM_022445) Human Untagged Clone

CAT#: SC111236

TPK1 (untagged)-Human thiamin pyrophosphokinase 1 (TPK1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TPK1
• Synonyms: HTPK1; PP20; THMD5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_022445, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCATGCCTTTACCCCGTTGGAGCCCCTGCTTTCCACTGGGAATTTGAAGTACTGCCTTGTAATTC
  TTAATCAGCCTTTGGACAACTATTTTCGTCATCTTTGGAACAAAGCTCTTTTAAGAGCCTGTGCCGATGG
  AGGTGCCAACCGCTTATATGATATCACCGAAGGAGAGAGAGAAAGCTTTTTGCCTGAATTCATCAATGGA
  GACTTTGATTCTATTAGGCCTGAAGTCAGAGAATACTATGCTACTAAGGGATGTGAGCTCATTTCAACTC
  CTGATCAAGACCACACTGACTTTACTAAGTGCCTTAAAATGCTCCAAAAGAAGATAGAAGAAAAAGACTT
  AAAGGTTGATGTGATCGTGACACTGGGAGGCCTTGCTGGGCGTTTTGACCAGATTATGGCATCTGTGAAT
  ACCTTGTTCCAAGCGACTCACATCACTCCTTTTCCAATTATAATAATCCAAGAGGAATCGCTGATCTACC
  TGCTCCAACCAGGAAAGCACAGGTTGCATGTAGACACTGGAATGGAGGGTGATTGGTGTGGCCTTATTCC
  TGTTGGACAGCCTTGTATGCAGGTTACAACCACAGGCCTCAAGTGGAACCTCACAAATGATGTGCTTGCT
  TTTGGAACATTGGTCAGTACTTCCAATACCTACGACGGGTCTGGTGTTGTGACTGTGGAAACTGACCACC
  CACTCCTCTGGACCATGGCCATCAAAAGCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022445 unedited
  GTCAAATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGNATCTCCA
  GTCTGTGGCTCCTACCAGCCATTGTAGGCCAATAATCCGTTATGGAGCATGCCTTTACCC
  CGTTGGAGCCCCTGCTTTCCACTGGGAATTTGAAGTACTGCCTTGTAATTCTTAATCAGC
  CTTTGGACAACTATTTTCGTCATCTTTGGAACAAAGCTCTTTTAAGAGCCTGTGCCGATG
  GAGGTGCCAACCGCTTATATGATATCACCGAAGGAGAGAGAGAAAGCTTTTTGCCTGAAT
  TCATCAATGGAGACTTTGATTCTATTAGGCCTGAAGTCAGAGAATACTATGCTACTAAGG
  GATGTGAGCTCATTTCAACTCCTGATCAAGACCACACTGACTTTACTAAGTGCCTTAAAA
  TGCTCCAAAAGAAGATAGAAGAAAAAGACTTAAAGGTTGATGTGATCGTGACACTGGGAG
  GCCTTGCTGGGCGTTTTGACCAGATTATGGCATCTGTGAATACCTTGTTCCAAGCGACTC
  ACATCACTCCTTTTCCAATTATAATAATCCAAGAGGAATCGCTGATCTACCTGCTCCAAC
  CAGGAAAGCACAGGTTGCATGTAGACACTGGAATGGAGGGTGATTGGTGTGGCCTTATTC
  CTGTTGGACAGCCTTGTATGCAGGTTACAACCACAGGCCTCAAGTGGAACCTCACAAATG
  ATGTGCTTGCTTTTGGAACATTGGTCAGTACTTCCAATACCTACGACGGGTCTGGTGTTG
  TGACTGTGGAAACTGACCACCCACTCCTCTGGACCATGGCCATCANAAGCTAACCTGTTG
  ACTGGCATCCATAAGTGTGCCTCTGCCTTATCTCATTNCTCACAGNTCATTGCTCNACAG
  AACGATNACCCTGGGTTNGCAGAATACTAACTCTCTAGGGGAACCCACTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022445 unedited
  GGGCAATCCAGCCATTTTTNNAANNGCGTCGTGAACGCGGCCGCATACTANAGCGAGTTT
  TTTTTTTTTTTTTTTGGAAAGTATCCATTTATTAACACTGGTAACATTTTGAGGGCACAC
  AGAACATGCATTCTTTGGTGGCAAAATTTTAAAATCACAGATATATTTCTTTTATATATT
  ATGAATATATTTCTAGTAAGGAATATATAACTAGTCTTCAGAAACTGAAGATGGAAACAT
  AGCTAAGCCTCAACTTCCGGTCAAGCAGAAAGTAATGAAAAGTGCCGAAAATGCCACAGA
  CACATGGGCCACTGCTCCTTAAATGCCACAATCACGCGGGCCACTGCTCCTTTCATTCTC
  TACAGACAACCTGAGTCTCACACTGTCATAGCACCAAGAAGAAAACAGTTACTTTCACTT
  TTCTTATAGCAACATAGGGATTTGTGTACATAAAACATGTTGGACAGGTTCTACTTCAAT
  CATGGTTTGTATGTCAACAGTTGGTTTCATAAAACAGAGAAAGGTGGAAAGAAGATAGAA
  GATGGTCTCTTTACCAAAGAAAGGTGAATACATTTCGATGCACCAGGTGTTTAGGCTAGT
  CAGTAACTGTGTGTATTATAGCCCTATGACTGCTAAGTGCAATATTTAAAATTCAGTGTG
  GGAAAGACCTGAGAAACCATTAGTCCAACCGCTTTCATTNTACAGCTAAGGAAAAAGGTT
  CCCGAAACTTAGGTGAATATCATACTGGGATGAAAACCCAAGCTTTGGAGTTAGGCTCCT
  TAGTTTGGAAGTTATGTTTCCCCATAAACAAACTAATTTCTTGTTAATATGAATCCCCTC
  TGGGAGAAACCAACCATTTTTAAGTCAAAGTGATTGTAAAAACAAATCAATTTTATGGTA
  AAGTTCCTTGGCCAACTTTTTTACTTAAGGGTGGGAAAAAGAATCAAACCGTTACCCCCC
  TTAAAACTTGGCCTTTTACTTTTGAAATTNGGGGTATAGGGGAATAAGGGCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022445
• Insert Size: 2560 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022445.2, NP_071890.2
• RefSeq Size: 2449 bp
• RefSeq ORF: 732 bp
• Locus ID: 27010
• UniProt ID: Q9H3S4
• Cytogenetics: 7q35
• Domains: TPK_catalytic, TPK_B1_binding
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Thiamine metabolism
• Gene Summary: The protein encoded by this gene functions as a homodimer and catalyzes the conversion of thiamine to thiamine pyrophosphate, a cofactor for some enzymes of the glycolytic and energy production pathways. Defects in this gene are a cause of thiamine metabolism dysfunction syndrome-5. [provided by RefSeq, Apr 2017]
  Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1 and 3 both encode the same isoform (a).