CDC42EP4 (NM_012121) Human Untagged Clone

CAT#: SC111209

CDC42EP4 (untagged)-Human CDC42 effector protein (Rho GTPase binding) 4 (CDC42EP4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC42EP4
• Synonyms: BORG4; CEP4; KAIA1777
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111209 sequence for NM_012121 edited (data generated by NextGen Sequencing)
  ATGCCAATCCTCAAGCAACTGGTGTCCAGCTCGGTGCACTCCAAGCGCCGTTCCCGAGCG
  GACCTCACGGCCGAGATGATCAGCGCCCCGCTGGGCGACTTCCGCCACACCATGCACGTT
  GGCCGGGCCGGAGACGCCTTTGGGGACACCTCCTTCCTCAATAGCAAGGCTGGCGAGCCC
  GACGGCGAGTCCTTGGACGAACAGCCCTCTTCTTCATCTTCCAAACGCAGTCTCCTGTCC
  AGGAAGTTCCGGGGCAGCAAGCGGTCACAGTCGGTGACCAGGGGGGAGCGGGAGCAGCGT
  GACATGCTGGGCTCCCTGCGGGACTCGGCCCTGTTTGTCAAGAATGCCATGTCCCTGCCC
  CAGCTCAATGAGAAGGAGGCCGCGGAGAAGGGCACCAGTAAGCTGCCCAAGAGCCTGTCA
  TCCAGCCCCGTGAAGAAGGCCAATGACGGGGAGGGCGGCGATGAGGAGGCGGGCACGGAG
  GAGGCAGTGCCCCGTCGGAATGGGGCCGCGGGTCCACATTCCCCTGACCCCCTCCTCGAT
  GAGCAGGCCTTTGGGGATCTGACAGATCTGCCTGTCGTGCCCAAGGCCACGTACGGGCTG
  AAGCATGCGGAGTCCATCATGTCCTTCCACATCGACCTGGGGCCCTCCATGCTGGGTGAC
  GTCCTCAGCATCATGGACAAGGAGGAGTGGGACCCCGAGGAGGGGGAGGGTGGTTACCAT
  GGCGATGAGGGCGCCGCTGGCACCATCACCCAGGCTCCCCCGTACGCCGTGGCGGCCCCT
  CCCCTGGCAAGGCAGGAAGGCAAGGCTGGCCCAGNCTTGCCCTCCCTCCCCTCCCATGCT
  CTGGAGGATAAGGGGTGGGCAGCAGCGGCCCCCAGCCCCGGCTCAGCCCGCAGCATGGGC
  AGCCACACCACACGGGACAGCAGCTCCCTCTCCAGCTGCACCTCAGGCATCCTGGAGGAG
  CGCAGCCCTGCCTTCCGGGGGCCGGACAGGGCCCGGGCTGCTGTCTCAAGACAGCCAGAC
  AAGGAGTTCTCCTTCATGGATGAGGAGGAGGAGGATGAAATCCGTGTGTGA
  
  Clone variation with respect to NM_012121.4
  815 a=>n;850 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012121 unedited
  TCACGAGGCCCAGCCGGGGTTACAAAACTGCCCCCTGCCCCCGGCGCGTCCCCCAGGAGC
  CATCCTGCCCGCGGGGATCCCGTCAGCCGCCTTATGATCTCCTCATTGCGTCCCCCTCTG
  CCCACTGGACTTGGACTTCAGATCTGACCCCAGACCTGCCGGCTACCTCGGGAGGGCCCA
  CCTCCCCGCCCATCCAGCAAGATGCCAATCCTCAAGCAACTGGTGTCCAGCTCGGTGCAC
  TCCAAGCGCCGTTCCCGAGCGGACCTCACGGCCGAGATGATCAGCGCCCCGCTGGGCGAC
  TTCCGCCACACCATGCACGTTGGCCGGGCCGGAGACGCCTTTGGGGACACCTCCTTCCTC
  AATAGCAAGGCTGGCGAGCCCGACGGCGAGTCCTTGGACGAACAGCCCTCTTCTTCATCT
  TCCAAACGCAGTCTCCTGTCCAGGAAGTTCCGGGGCAGCAAGCGGTCACAGTCGGTGACC
  AGGGGGGAGCGGGAGCAGCGTGACATGCTGGGCTCCCTGCGGGACTCGGCCCTGTTTGTC
  AAGAATGCCATGTCCCTGCCCCAGCTCAATGAGAAGGAGGCCGCGGAGAAGGGCACCAGT
  AAGCTGCCCAAGAGCCTGTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012121 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTGCTTTAAGGCTTTTT
  TTTTTAATGTTTCCTCACTGTTTTGACAATATCATGAAAAAAATCAGTTTAAAATCTGGA
  ATTGGCCTGGACGGGATTCGAGGGCACCTGGCGGGGGCTGCATAACCCCTGAGGTTCCTC
  CCCCACCATGGGACCTAAGCTATTGGAAACAGGAGCACCAACAGGGCACCGAACCTGGAA
  CTAAGCTACCGCCTACAGCCACGCACCATAGGATAGTCCCGCAACACAGGAAGGGCCCGG
  TCAAACCACCCCTGGCGACGATAACCGGCTGCTTCAGCCCCCACCACCCCATCAGCCCCC
  TAACCGACTATTGACAGTTCTTCCTTTTGCCTCCTTATTCACACTCCCATTACCTTCTCC
  CGACTACTTTATGCTCTTCCGCACTCTCCCCCCCATTATTACCCGATAAATGCCATCACA
  CTCGCCCCTCCAGCGCGACGCAATCAACAAAGTACATGAAAATTTTCACCGCACCCCACC
  ATGACACACACATGTTCCACTTACTTTGCCCTACTCCATGTTCCCCTTATTATCACCCCC
  AATCCCCAACATATCAACCTTATACATACCCCCCCCCTCTCATCCCCACACTCCACCGAA
  TCTATCGTTCACTTAAGTTTTTCCTTAGTGTCCTGCGCGTAGATTACTCCATTCCCCAAT
  ACATACAACATTAATTAGATCCATGAAAACCCCGCGCCCGATAAAGTAANCTATACTCTA
  ACCCTAGCCGATTAACACCATACTTAACCACGCATATACTAATCCCCTACCCCCGACCTA
  CTCCCCTCCTCTCCTCACCAACAACCAAATGAATTACCCATAACCTCTCACAATCCACCA
  TCAATACCTCCCGTATTGCCATATCACTCCACATN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012121
• Insert Size: 3180 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012121.4, NP_036253.2
• RefSeq Size: 3121 bp
• RefSeq ORF: 1071 bp
• Locus ID: 23580
• UniProt ID: Q9H3Q1
• Cytogenetics: 17q25.1
• Domains: PBD
• Gene Summary: The product of this gene is a member of the CDC42-binding protein family. Members of this family interact with Rho family GTPases and regulate the organization of the actin cytoskeleton. This protein has been shown to bind both CDC42 and TC10 GTPases in a GTP-dependent manner. When overexpressed in fibroblasts, this protein was able to induce pseudopodia formation, which suggested a role in inducing actin filament assembly and cell shape control. [provided by RefSeq, Jul 2008]