PIGL (NM_004278) Human Untagged Clone

CAT#: SC111117

PIGL (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class L (PIGL)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIGL
• Synonyms: CHIME
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004278, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAGCAATGTGGCTCCTGTGTGTGGCGTTGGCGGTCTTGGCATGGGGCTTCCTCTGGGTTTGGGACT
  CCTCAGAACGAATGAAGAGTCGGGAGCAGGGAGGACGGCTGGGAGCCGAAAGCCGGACCCTGCTGGTCAT
  AGCGCACCCTGACGATGAAGCCATGTTTTTTGCTCCCACAGTGCTAGGCTTGGCCCGCCTAAGGCACTGG
  GTGTACCTGCTTTGCTTCTCTGCAGGAAATTACTACAATCAAGGAGAGACTCGTAAGAAAGAACTTTTGC
  AGAGCTGTGATGTTTTGGGGATTCCACTCTCCAGTGTAATGATTATTGACAACAGGGATTTCCCAGATGA
  CCCAGGCATGCAGTGGGACACAGAGCACGTGGCCAGAGTCCTCCTTCAGCACATAGAAGTGAATGGCATC
  AATCTGGTGGTGACTTTCGATGCAGGGGGAGTAAGTGGCCACAGCAATCACATTGCTCTGTATGCAGCTG
  TGAGGGCCCTGCACTCAGAAGGGAAGTTACCTAAAGGGTGCTCTGTGCTCACGCTTCAGTCTGTGAATGT
  GCTGCGCAAGTACATCTCCCTTCTGGATCTGCCCTTGTCTCTGCTTCATACGCAGGATGTCCTCTTCGTG
  CTCAACAGCAAAGAAGTGGCACAGGCCAAGAAAGCCATGTCCTGCCACCGCAGCCAGCTCCTCTGGTTCC
  GCCGCCTCTACATTATCTTCTCCCGGTACATGAGAATCAACTCACTGAGCTTCCTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004278 unedited
  TTCAGATTTGTATCCGACTCACTATAGGGCGGCCGCGCATTCGGCACGAGGCAATGTGGC
  TCCTGTGTGTGGCGTTGGCGGTCTTGGCATGGGGCTTCCTCTGGGTTTGGGACTCCTCAG
  AACGAATGAAGAGTCGGGAGCAGGGAGGACGGCTGGGAGCCGAAAGCCGGACCCTGCTGG
  TCATAGCGCACCCTGACGATGAAGCCATGTTTTTTGCTCCCACAGTGCTAGGCTTGGCCC
  GCCTAAGGCACTGGGTGTACCTGCTTTGCTTCTCTGCAGGAAATTACTACAATCAAGGAG
  AGACTCGTAAGAAAGAACTTTTGCAGAGCTGTGATGTTTTGGGGATTCCACTCTCCAGTG
  TAATGATTATTGACAACAGGGATTTCCCAGATGACCCAGGCATGCAGTGGGACACAGAGC
  ACGTGGCCAGAGTCCTCCTTCAGCACATAGAAGTGAATGGCATCAATCTGGTGGTGACTT
  TCGATGCAGGGGGAGTAAGTGGCCACAGCAATCACATTGCTCTGTATGCAGCTGTGAGGG
  CCCTGCACTCAGAAGGGAAGTTACCTAAAGGTAAGGCTTGTTCCTTTTGCAAAGGGCCAC
  AAGATACTGTCCCTCTGAGAAACTTATGAGGGGGAAATTTGCAAATCCACAGAGAGCTGC
  CCTCAGCCGAGCAGAGGCAGTGGTTGGGGAGGCCGNCACACTCTTTCTGACTCACCTCAA
  GGTACCTGGCAGGTTGTATCTACCAGCAAGTGCCTGCCTGCANGGCCACCTCTGGATGAG
  TGGGGAGGCCACGTTTGCCCCTCACCTGTCTAGCTTTTCACAGGGTGGATGTTTTACACC
  TCTGGTATTCTCCAGTCTGAACTCAATCCCATTCCCCACCAATAAGCAGGTTGTAATGAC
  TGCTGGGGGCTTGATGACCCATCTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004278 unedited
  ATGAACCGCGGCCCGCTTCTAGNATCGAGTTTTTTTTTTTTTTTTTTGTTTTTAGAGCAC
  TTTTATGTTCATTTATGATAATAGGGACCTGCCCAGGCACTGGGCTAGGTGTTCCACATC
  TACTGTGTCATTATGTAAATTGTTTTTCACAAGAACTTGAGAAGCTAGCAGTGTAGCTAT
  TATTATCCTTGGGGTGGTTTGTTTTTGGTTCTTTGGGTTTTTTCCCAGGGGAAGAAGCTG
  GAGTTTGGACAGCCCCTGGCCTACATGGGCTCCCTTTTGCAGAGGCTGCTCCAGCGGGGA
  TCTCCTTGAGCTCAGGTAAATAAGCCAGTGCCAGGCCAGGTCCTCTGCACTCCTTTGTCT
  ATTTTCCCCTTTGTTCCTTGGACCTGAAAACCCTTCAAGGCTTCAGAGGAAGCTCAGTGA
  GTTGATTCTCATGTACCGGGAGAAGATAATGCAGAGGCGGCGGAACCAGAGGAGCTGGCT
  GCGGTGGCAGGACATGGCTTTCTTGGCCTGTGCCACTTCTTTGCTGTTGAGCACGAAGAG
  GACATCCTGCGTATGAAGCAGAGACAAGGGCAGATCCAGAAGGGAGATGTACTTGCGCAG
  CACATTCACAGACTGAAGCGTGAGCACAGAGCACCCTGGATGGAGAGGAGACAGGGTGAA
  GCGATAAGCCTTGAGGCCTCCAGCCAGATTCTCCCCTGAGCATCCTCCCTCTACCCTGTG
  GCTCAAGGCAGGGCAGGACAGTGGCCACAGCATTGCCCCGTGTTTCTGTGGCACTACAGC
  TGCCNTGTGCCCATGATTCTTGCAGCTAGAACGAGTATAAGGGCCATAGAAGACCTGGCT
  TGCTACCCTCTACCCCTGCGGTGTGCCCTACCANAAAAACGTTGTCTGCTTACAGCGTGA
  AACTTTGCTTACCTCAAAGCCAAAGCAAGTAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004278
• Insert Size: 2090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004278.2, NP_004269.1
• RefSeq Size: 1163 bp
• RefSeq ORF: 759 bp
• Locus ID: 9487
• UniProt ID: Q9Y2B2
• Cytogenetics: 17p11.2
• Protein Families: Transmembrane
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways
• Gene Summary: This gene encodes an enzyme that catalyzes the second step of glycosylphosphatidylinositol (GPI) biosynthesis, which is the de-N-acetylation of N-acetylglucosaminylphosphatidylinositol (GlcNAc-PI). Study of a similar rat enzyme suggests that this protein localizes to the endoplasmic reticulum. [provided by RefSeq, Jul 2008]