Pirin (PIR) (NM_003662) Human Untagged Clone

CAT#: SC111077

PIR (untagged)-Human pirin (iron-binding nuclear protein) (PIR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Pirin
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111077 sequence for NM_003662 edited (data generated by NextGen Sequencing)
  ATGGGGTCCTCCAAGAAAGTTACTCTCTCAGTGCTCAGCCGGGAGCAGTCGGAAGGGGTT
  GGAGCGAGGGTCCGGAGAAGCATTGGCAGACCCGAGTTAAAAAATCTGGATCCGTTTTTA
  CTGTTTGATGAATTTAAAGGAGGTAGACCAGGAGGATTTCCTGATCATCCACATCGAGGT
  TTTGAAACAGTATCCTACCTCCTGGAAGGGGGCAGCATGGCCCATGAAGACTTCTGTGGA
  CACACTGGTAAAATGAACCCAGGAGATTTGCAGTGGATGACTGCGGGCCGGGGCATTCTG
  CACGCTGAGATGCCTTGCTCAGAGGAGCCAGCCCATGGCCTACAACTGTGGGTTAATTTG
  AGGAGCTCAGAGAAGATGGTGGAGCCTCAGTACCAGGAACTGAAAAGTGAAGAAATCCCT
  AAACCCAGTAAGGATGGTGTGACAGTTGCTGTCATTTCTGGAGAAGCCCTGGGAATAAAG
  TCCAAGGTTTACACTCGCACACCAACCTTATATTTGGACTTCAAATTGGACCCAGGAGCC
  AAACATTCCCAACCTATCCCTAAAGGGTGGACAAGCTTCATTTACACGATATCTGGAGAT
  GTGTATATTGGGCCCGATGATGCACAACAAAAAATAGAACCTCATCACACAGCAGTGCTT
  GGAGAAGGTGACAGTGTCCAAGTGGAGAACAAGGATCCCAAGAGAAGCCACTTTGTCTTA
  ATTGCTGGGGAGCCATTAAGAGAACCAGTTATCCAACATGGTCCATTTGTGATGAACACC
  AATGAAGAGATTTCTCAAGCTATTCTTGATTTCAGAAACGCAAAAAATGGGTTTGAAAGG
  GCCAAAACCTGGAAATCAAAGATTGGGAACTAG
  
  Clone variation with respect to NM_003662.3
  681 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003662 unedited
  TCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCCGCTAAGGCTCCAGGTGCCGCTA
  CCGCAGCCCCTCCATCCTCTACAGCTCAGCATCAGAACACTCTCTTTTTAGACTCCGATA
  TGGGGTCCTCCAAGAAAGTTACTCTCTCAGTGCTCAGCCGGGAGCAGTCGGAAGGGGTTG
  GAGCGAGGGTCCGGAGAAGCATTGGCAGACCCGAGTTAAAAAATCTGGATCCGTTTTTAC
  TGTTTGATGAATTTAAAGGAGGTAGACCAGGAGGATTTCCTGATCATCCACATCGAGGTT
  TTGAAACAGTATCCTACCTCCTGGAAGGGGGCAGCATGGCCCATGAAGACTTCTGTGGAC
  ACACTGGTAAAATGAACCCAGGAGATTTGCAGTGGATGACTGCGGGCCGGGGCATTCTGC
  ACGCTGAGATGCCTTGCTCAGAGGAGCCAGCCCATGGCCTACAACTGTGGGTTAATTTGA
  GGAGCTCAGAGAAGATGGTGGAGCCTCAGTACCAGGAACTGAAAAGTGAAGAAATCCCTA
  AACCCAGTAAGGATGGTGTGACAGTTGCTGTCATTTCTGGAGAAGCCCTGNGAATAAAGT
  CCAAGGTTTACACTCGCACACCAACCTTATATTTGGACTTCAAATTGGACCCAGGAGCCA
  AACATTCCCAACCTATCCCTAAAGGGTGGNACAGCTTCATTTACACGATATCTGGNAGAT
  GTGTATATTTGGGCCCCGATGATGCACAACAAAAATAGAACCTCATCACACAGCAGTGCT
  TGGNAGAAGGGTGACAGTNGTCCAAGTGGAGAACAAGGGATCCCNAGAGAAGCCACCTTG
  TTCTTAATGCTGGGGGAGCATTANGAGAACCAGNTATCCANCTTGTNCCATTNGGGATGA
  ACACCATGNAGAGAATTCTCCAGCTTTTNCTTGATTTCNAACCCGAAANAATGGGGTTTG
  AANGGGCCCAAACCCCTGAATCAAGATGGGGACTANTGAANCGGNNNNAAACGGGCTTTG
  GAGGGGCCTCAATATTGCATTNTTGGGATGAGCACTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003662 unedited
  GCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAAAAACAATGATTAG
  GTTTATTTGCATGTGCCAGGAAATATCCTACATTTATTGTTACAAAAACCATGTTATCAC
  GTTAGTGTGGAATTCTTTAGAAGCACCGGCTAAATAAGCTTTAGAAATGGAATGCCTTCA
  ATGGCTCAATCTCAGAAATGGCAAAATTCTAGGACACATCAAGACCTGCTCTTCCGCTTT
  CCACTAGTTCCCAATCTTTGATTTCCAGGTTTTGGCCCTTTCAAACCCATTTTTTGCGTT
  TCTGAAATCAAGAATAGCTTGAGAAATCTCTTCATTGGTGTTCATCACAAATGGACCATG
  TTGGATAACTGGTTCTCTTAATGGCTCCCCAGCAATTAAGACAAAGTGGCTTCTCTTGGG
  ATCCTTGTTCTCCACTTGGACACTGTCACCTTCTCCAAGCACTGCTGTGTGATGAGGTTC
  TATTTTTTGTTGTGCATCATCGGGCCCAATATACACATCTCCAGATATCGTGTAAATGAA
  GCTTGTCCACCCTTTAGGGATAGGTTGGGAATGTTTGGCTCCTGGGTCCAATTTGAAGTC
  CAAATATAAGGTTGGTGTGCGAGTGTAAACCTTGGACTTTATTCCCAGGGCTTCTCCAGA
  AATGACAGCAACTGTCACACCATCCTTACTGGGTTTAGGGATTTCTTCACTTTTCAGTTC
  CTGGTACTGAAGCTCCCACCATCTTCTCTGAGCTCCCTCAATTACCCACAGTTGTANGCC
  ATGGGCTGGCCTCTCTGAGCAAGCATCTTACGTGCAAATGCCCCGCCCGCATCATTCCTG
  CAATCTCCTGGGTTATTTACCATGGTCCCAGAAGTTTATGGCCTGCTGCCCCTTCCAGAG
  GTAGAACCGTNTAAACCCATGGGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003662
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003662.2, NP_003653.1
• RefSeq Size: 1316 bp
• RefSeq ORF: 873 bp
• Locus ID: 8544
• UniProt ID: O00625
• Cytogenetics: Xp22.2
• Domains: DUF209
• Protein Families: Transcription Factors
• Gene Summary: This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.