PIP5K1 alpha (PIP5K1A) (NM_003557) Human Untagged Clone

CAT#: SC111072

PIP5K1A (untagged)-Human phosphatidylinositol-4-phosphate 5-kinase, type I, alpha (PIP5K1A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIP5K1 alpha
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111072 sequence for NM_003557 edited (data generated by NextGen Sequencing)
  ATGGCGTCGGCCTCCTCCGGGCCGTCGTCTTCGGTCGGTTTTTCATCCTTTGATCCCGCG
  GTCCCTTCCTGTACCTTGTCCTCAGCATCTGGAATCAAGAGACCCATGGCATCTGAGGTG
  CCTTATGCCTCTGGCATGCCCATCAAGAAAATAGGCCATAGAAGTGTTGATTCCTCAGGA
  GAGACAACATATAAAAAGACAACCTCATCAGCCTTGAAAGGTGCCATCCAGTTAGGCATT
  ACCCACACTGTGGGGAGCCTGAGTACCAAACCAGAGCGTGATGTCCTCATGCAAGATTTC
  TACGTGGTTGAGAGTATCTTCTTTCCCAGTGAAGGGAGCAACCTGACCCCTGCTCATCAC
  TACAATGACTTTCGTTTCAAGACCTATGCACCTGTTGCCTTCCGCTACTTCCGGGAGCTA
  TTTGGTATCCGGCCCGATGATTACTTGTATTCCCTCTGCAGTGAGCCGCTGATTGAACTC
  TGTAGCTCTGGAGCTAGTGGTTCCCTATTCTATGTGTCCAGCGACGATGAGTTCATTATT
  AAGACAGTCCAACATAAAGAGGCGGAATTTCTGCAGAAGCTGCTTCCAGGATACTACATG
  AACCTCAACCAGAACCCTCGGACTTTGCTGCCTAAATTCTATGGACTGTACTGTGTGCAG
  GCAGGTGGCAAGAACATTCGGATTGTGGTGATGAACAATCTTTTACCAAGATCGGTAAAA
  ATGCATATCAAATATGACCTCAAAGGCTCAACCTACAAACGGCGGGCTTCCCAGAAAGAG
  CGAGAGAAGCCTCTTCCCACATTTAAAGACCTAGACTTCTTACAAGACATCCCTGATGGT
  CTTTTTTTGGATGCTGACATGTACAACGCTCTCTGTAAGACCCTGCAGCGTGACTGTTTG
  GTGCTGCAGAGCTTCAAGATAATGGATTATAGCCTCTTGATGTCAATCCATAATATAGAT
  CATGCACAACGAGAGCCCTTAAGCAGTGAAACACAGTACTCAGTTGATACTCGAAGACCG
  GCCCCCCAAAAGGCTCTGTATTCCACAGCCATGGAATCCATCCAGGGAGAGGCTCGACGG
  GGTGGTACCATGGAGACTGATGACCATATGGGTGGCATCCCTGCCCGGAATAGTAAAGGG
  GAAAGGCTTCTGCTTTATATTGGCATCATTGACATTCTACAGTCTTACAGGTTTGTTAAG
  AAGTTGGAGCACTCTTGGAAAGCCCTGGTACATGACGGAGACACTGTCTCAGTGCATCGC
  CCAGGCTTCTACGCTGAACGGTTCCAGCGCTTCATGTGCAACACAGTATTTAAGAAGATT
  CCCTTGAAGCCTTCTCCTTCCAAAAAGTTTCGGTCTGGCTCATCTTTCTCTCGGCGAGCA
  GGCTCCAGTGGCAACTCCTGCATTACTTACCAGCCATCGGTCTCTGGGGAACACAAGGCA
  CAAGTGACAACAAAGGCAGAAGTGGAGCCAGGCGTTCACCTTGGTCGTCCTGATGTTTTA
  CCTCAGACTCCACCTTTGGAGGAAATCAGTGAGGGCTCGCCTATTCCTGACCCCAGTTTC
  TCACCTCTGGTTGGAGAGACTTTGCAAATGCTAACTACAAGTACAACCTTGGAAAAGCTT
  GAAGTTGCAGAGTCAGAGTTCACCCATTAA
  
  Clone variation with respect to NM_003557.2
  930 c=>t;1569 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003557 unedited
  GGGGTTCGGGATTTATGTATACGACTCACTATAGNNGGCGGCCGCGCAATTCGGCACCAG
  GTTAGGAAGGACGGAGAAGGGCGTTCGCTCCTTTGGGACTTTTCATGCCTCGTTTTTTTT
  TCAGATGTGGCTTGGTCTGGGCGCAAGGTCCCAGCAGCCAGCTTAAGCTTACTCTTCTGT
  GAAAGGGGAAAGTATCCCCTGTGGAAAGCGGTTAAACTTGTGGAGGGGGTGCGGGACGTG
  AGTTCTTCCCCATGCCAGGCGAATGGTGTGGCCTTGAGCTGGTCCAGGAGCCGGCTCGAC
  GTGTCTGAGGGAGGCCCCGGAGGGGGCGGGGAGGTGGCCCACAGAACGCGGGTTCTGTAA
  AGAGACGTTGGGAAGATTCGATTCCGAGAAGAGGAAGAACCGGATTGAAAGAGAGCCAGG
  CCGCTGAGGGGGAGGGGGCTGCTAAGATGGCGTCGGCCTCCTCCGGGCCGTCGTCTTCGG
  TCGGTTTTTCATCCTTTGATCCCGCGGTCCCTTCCTGTACCTTGTCCTCAGCATCTGGAA
  TCAAGAGACCCATGGCATCTGAGGTGCCTTATGCCTCTGGCATGCCCATCAAGAAAATAG
  GCCATAGAAGTGTTGATTCCTCAGGAGAGACAACATATAAAAAGACAACCTCATCAGCCT
  TGAAAGGTGCCATCCAGTTAGGCATTACCCACACTGTGGGGAGCCTGAGTACCAAACCAG
  AGCGTGATGTCCTCATGCAAGATTTCTACGTGGTTGAGAGTATCTTCTTTCCCAGTGAAG
  GGAGCAACCTNGACCCTGCTCATCACTACAATGACCTTCCGTTTCAGGACCTATGCACCT
  GTTGCCTTCCGCTACCTCCGGAGCTATTTGGNATCCCGCCCCGAGATTACTTGTATTCCC
  TCTGCAGGGAGCCGCTGTATGACTCTGTAACTCTGAACTAGGGCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003557 unedited
  NGGTTAACTGCTATAAGTTTATTACATTAAAACCTATATCACTTTNTAATTGTATTTGCC
  ATTTCTGAACAATACAAAGTAGAGGCAAATAGTAACACTTAATAAAAATGTTGCTTCCCC
  ATTCCTTTCCCTAAGACCCAGCCCTCCCTCAAATATTCCCTCCCACCCCTAACAGAACTC
  TGTCCTTAATAAAACACTTAAATACATCATAAATAGTAAATTATGAAAAAAAAATAGCAA
  GAATTCTTTCCTTCTTGTAAAAAACATGGCAGCAAAGACAGGCAGCCAGAAACTGGCTGG
  GGGTGTCTAATATAGACAGTTATTTGGGGTATGGGCTAACATATTATCAAGGCAGGGTGG
  GATAGAGAAGGGATAGAACAGGAATTCAACAATCTGCCTGAGATACCAGGCCCCCTTCTT
  CCCCTGACAAGAGTGGAAGGATCAGATGGAAAACCACAACTCTTGTAAACGGGGAGGAGA
  GGGCATATGTAGCCCCCATTTCCAACTTCCCTGCATGAAGACAGGGGGGTAGGACATATG
  GCACAGCAATGACCTCACCAAGGCAAGAATCTGCTGTCTCCCCATGAGTTTAAGAAAATA
  TTTGCTGGGCCTCACAGATATGGACTGTCTGTTAAACAGACAGAAGTGTCCCCTCTTTAC
  CTTACCTNCACANACAGGAGAACCACTGGTNGGAAAAGATGAGAAGGAGCAAAACACATC
  TCCTCTGGAGAAGGACCAAAATTCATTTGGGTAGGTCTCACATTTAGAAAACCACCCGGA
  TATATTTCCAAATTGCCAAATATAATAATTACTATAGCCCGGGCGTGAACAGGATCTTGC
  CTGGGAGGGGCTTGACCTCCAACCCAGGGTTGCCTCCGGGTTTACCAAAAGGGAAAAGAG
  AACCCCCTGGGCCCATTTTTTTATTCCTTAAGCCCTAGAAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003557
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003557.1, NP_003548.1
• RefSeq Size: 3713 bp
• RefSeq ORF: 1650 bp
• Locus ID: 8394
• UniProt ID: Q99755
• Cytogenetics: 1q21.3
• Domains: PIP5K
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of actin cytoskeleton
• Gene Summary: Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). PtdIns(4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), another second messenger. The majority of PtdIns(4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs (PubMed:18288197, PubMed:19158393, PubMed:20660631). Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks 3 nt at an alternate splice site and an in-frame exon in the CDS, as compared to variant 1. The resulting isoform (2) is shorter but has identical N- and C-termini, as compared to isoform 1.