Triosephosphate isomerase (TPI1) (NM_000365) Human Untagged Clone

CAT#: SC111041

TPI1 (untagged)-Human triosephosphate isomerase 1 (TPI1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Triosephosphate isomerase
• Synonyms: HEL-S-49; TIM; TPI; TPID
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111041 sequence for NM_000365 edited (data generated by NextGen Sequencing)
  ATGGCGCCCTCCAGGAAGTTCTTCGTTGGGGGAAACTGGAAGATGAACGGGCGGAAGCAG
  AGTCTGGGGGAGCTCATCGGCACTCTGAACGCGGCCAAGGTGCCGGCCGACACCGAGGTG
  GTTTGTGCTCCCCCTACTGCCTATATCGACTTCGCCCGGCAGAAGCTAGATCCCAAGATT
  GCTGTGGCTGCGCAGAACTGCTACAAAGTGACTAATGGGGCTTTTACTGGGGAGATCAGC
  CCTGGCATGATCAAAGACTGCGGAGCCACGTGGGTGGTCCTGGGGCACTCAGAGAGAAGG
  CATGTCTTTGGGGAGTCAGATGAGCTGATTGGGCAGAAAGTGGCCCATGCTCTGGCAGAG
  GGACTCGGAGTAATCGCCTGCATTGGGGAGAAGCTAGATGAAAGGGAAGCTGGCATCACT
  GAGAAGGTTGTTTTCGAGCAGACAAAGGTCATCGCAGATAACGTGAAGGACTGGAGCAAG
  GTCGTCCTGGCCTATGAGCCTGTGTGGGCCATTGGTACTGGCAAGACTGCAACACCCCAA
  CAGGCCCAGGAAGTACACGAGAAGCTCCGAGGATGGCTGAAGTCCAACGTCTCTGATGCG
  GTGGCTCAGAGCACCCGTATCATTTATGGAGGCTCTGTGACTGGGGCAACCTGCAAGGAG
  CTGGCCAGCCAGCCTGATGTGGATGGCTTCCTTGTGGGTGGTGCTTCCCTCAAGCCCGAA
  TTCGTGGACATCATCAATGCCAAACAATGA
  
  Clone variation with respect to NM_000365.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000365 unedited
  GTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGCAATTCGCACGAGTCGGCTCCGC
  GCCTGGCGCCCTCCAGGAATTCTTCGTTGGGGGAAACTGGAAGATGAACGGGCGGAAGCA
  GAGTCTGGGGGAGCTCATCGGCACTCTGAACGCGGCCAAGGTGCCGGCCGACACCGAGGT
  GGTTTGTGCTCCCCCTACTGCCTATATCGACTTCGCCCGGCAGAAGCTAGATCCCAAGAT
  TGCTGTGGCTGCGCAGAACTGCTACAAAGTGACTAATGGGGCTTTTACTGGGGAGATCAG
  CCCTGGCATGATCAAAGACTGCGGAGCCACGTGGGTGGTCCTGGGGCACTCAGAGAGAAG
  GCATGTCTTTGGGGAGTCAGATGAGCTGATTGGGCAGAAAGTGGCCCATGCTCTGGCAGA
  GGGACTCGGAGTAATCGCCTGCATTGGGGAGAAGCTAGATGAAAGGGAAGCTGGCATCAC
  TGAGAAGGTTGTTTTCGAGCAGACAAAGGTCATCGCAGATAACGTGAAGGACTGGAGCAA
  GGTCGTCCTGGCCTATGAGCCTGTGTGGGCCATTGGTACTGGCAAGACTGCAACACCCCA
  ACAGGCCCAGGAAGTACACGAGAAGCTCCGAGGATGGCTGAAGTCCAACGTCTCTGATGC
  GGTGGCTCAGAGCACCCGTATCATTTATNGGAGCTCTGTGACTGGGGCAACCTGCAGGGA
  GCTGGCCAGCCAGCCTGAGTTGGATGGCTTCCTTGTGGGGTGTTGCTTCCCTCAGCCCGA
  ATTCGTNGACATCATCAATGCCAAAACATGAGCCCATNCATCTTCCCTACCCTTCCTGCC
  CAGCCAGGGACTAAGCAGNCCAGAAGCCCAGTACTGCCCTTNCCTGCATATGCTCTGATG
  GGNCATCTGCTCCTTCTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000365 unedited
  GTGGCAACTTTCCAGGGCCAGGAAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGG
  ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTCCTAGGGCCAGGTTTATTCCCTCACATGGGGGGTTCACAT
  ACACAGCACAGAGGCACGGGCACCATGGGAAAGGGCAGCACTCCTGCCTTTTGAGGGGAT
  CTTGGCCTCACGGGGTAAAAAGGGAAAGGATGGTTTCTCTTCTGCCCTCACTAGGGCCTA
  GGGAACCCAGGAGCAAATCCCACCACGCCTTCCATTTTTAAGCCAAGGAAAAGCCACCTT
  GGGGACGTTTAGTTCCAACCATTATAGTAAGGGGAAAAGGGATTGGCCTGGTCCCAACCA
  TTACAGGGGGAAGATATAAACAGTAAAGGAAGATACAGTTTGGATGAGGCCACAGGAAGG
  AGCAAATGACACCATCAAAACCATATGCAGGGAAAGGGCAGTTACTGGGCTTCTGGGCTG
  CTTANTCCCTGGCTTGGCAGGAAGGGTAGGGAAAATGGATGGGGCTCATTGTTTGGCATT
  GATGATGTCCACAAATTCGGGCTTGAGGGAAGCACCACCCACAAGGAAGCCATCCACATC
  AGGCTGGCTGGCCAGCTCCTTGCAGGTTGCCCCAGTCACAGAGCCTCCATAAATGATACG
  GGTGCTCTGAGCCACCGATNAGAGACGTTGGACTTCAGCCTCCTCGGAGCTTCTCGGTAC
  TTCTGGGCTGTTTNGGGGGTNTTTAAATTTTGCCAGTACCAATGGCCCACACAGCTCATA
  GCCAGACACTTGCTCANCCTTCAGTTATTGGAAGGACTTTGTTGCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000365
• Insert Size: 1340 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000365.4, NP_000356.1
• RefSeq Size: 1254 bp
• RefSeq ORF: 750 bp
• Locus ID: 7167
• UniProt ID: P60174
• Cytogenetics: 12p13.31
• Domains: TIM
• Protein Pathways: Fructose and mannose metabolism, Glycolysis / Gluconeogenesis, Inositol phosphate metabolism, Metabolic pathways
• Gene Summary: This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
  Transcript Variant: This variant (1) encodes the predominant isoform (1). CCDS Note: This CCDS represents a TPI variant that uses an internal promoter compared to the CCDS53740.1 representation. Data in PMIDs 4022011, 2925688, 2243103 and 10575546 support the presence of the internal promoter used by this variant. The resulting isoform is 37 aa shorter at the N-terminus compared to the CCDS53740.1 isoform. N-terminal sequencing in PMIDs 9150946 and 9150948 supports the existence of the shorter isoform in vivo.