SIAT4A (ST3GAL1) (NM_173344) Human Untagged Clone

CAT#: SC111017

ST3GAL1 (untagged)-Human ST3 beta-galactoside alpha-2,3-sialyltransferase 1 (ST3GAL1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SIAT4A
• Synonyms: 1; Gal-NAc6S; SIAT4A; SIATFL; ST3GalA; ST3GalA.1; ST3GalIA; ST3O
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_173344, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGACCCTGCGGAAGAGGACCCTGAAAGTGCTCACCTTCCTCGTGCTCTTCATCTTCCTCACCTCCT
  TCTTCCTGAACTACTCCCACACCATGGTGGCCACCACCTGGTTCCCCAAGCAGATGGTCCTGGAGCTCTC
  CGAGAACCTGAAGAGACTGATCAAGCACAGGCCTTGCACCTGCACCCACTGCATCGGGCAGCGCAAGCTC
  TCGGCCTGGTTCGATGAGAGGTTCAACCAGACCATGCAGCCGCTGCTGACCGCCCAGAACGCGCTCTTGG
  AGGACGACACCTACCGATGGTGGCTGAGGCTCCAGCGGGAGAAGAAGCCCAATAACTTGAATGACACCAT
  CAAGGAGCTGTTCAGAGTGGTGCCTGGGAATGTGGACCCTATGCTGGAGAAGAGGTCGGTGGGCTGCCGG
  CGCTGCGCCGTTGTGGGCAACTCGGGCAACCTGAGGGAGTCTTCTTATGGGCCTGAGATAGACAGTCACG
  ACTTTGTCCTCAGGATGAACAAGGCGCCCACGGCAGGGTTTGAAGCTGATGTTGGGACCAAGACCACCCA
  CCATCTGGTGTACCCTGAGAGCTTCCGGGAGCTGGGAGATAATGTCAGCATGATCCTGGTGCCCTTCAAG
  ACCATCGACTTGGAGTGGGTGGTGAGCGCCATCACCACGGGCACCATTTCCCACACCTACATCCCGGTTC
  CTGCAAAGATCAGAGTGAAACAGGATAAGATCCTGATCTACCACCCAGCCTTCATCAAGTATGTCTTTGA
  CAACTGGCTGCAAGGGCACGGGCGATACCCATCTACCGGCATCCTCTCGGTCATCTTCTCAATGCATGTC
  TGCGATGAGGTGGACTTGTACGGCTTCGGGGCAGACAGCAAAGGGAACTGGCACCACTACTGGGAGAACA
  ACCCATCCGCGGGGGCTTTTCGCAAGACGGGGGTGCACGATGCAGACTTTGAGTCTAACGTGACGGCCAC
  CTTGGCCTCCATCAATAAAATCCGGATCTTCAAGGGGAGATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_173344 unedited
  TTTGTAATACGACTTATTATAGGGCGGCCGCGAATTCGGCACGAGGCAAAGACAAAGAGC
  CCTAGACCGGAGGGAGGAGGAGGAGGAAGAAGAGCGGAGAGAGAAGGAAGAGGCGATGTG
  AGCTGGGAAGGGGGCAAGTGTCCGGGACACCCACACCCCTGTATTCTCCTCCGAACCCCT
  TCATGCCCAAATCCCGGAAACTCCAGCGTGTCTCCAGCCGTGTTGGTACCATTTTCAGAT
  TTCATCTTCCTAAACTGGAAATGTCAATGAGAGGAAATTAACACCCCCAAGAGCTGCAGT
  GAGCAAATGCATTGAGCTTGGGTCAGGACAATTCCATTTGGGGACCAGAGATGGACGGTC
  ACTCAGCCTATGGAGATGAAGAAACTGAGGTTCAGAGAGGTTAAGAGACTCCACTGAGGT
  CACACAGCCGATGACAGACAACCTTCTGTGCCTTCATCAAGCTGGTTGTGTACCCACCAT
  GTCCCTGGCGACAGGATGGGAAAGAAAAAGCCCTAATTAAGGATCGTCAGAAACCACAGT
  TGGAGGAGGACGGCAGAGACAGTTTCCCTCCCCGCTATACCAACACCCTTCCTTCGAGGT
  CCTCGCTCCTGAGGGACCCTGGACTGTCACAGAGATTAATGACCCCTTATCTTCTTTGGA
  TGTGAAAGGAAATCACTGGTTAAAGCTTGATCGAGAGACATTATCAGCTCTTTAAGGATT
  GCAGGAGAATAGGCTACTTTATTTTCTGAAAGGNAGGGAGTTCCTGCTACCCATCGTGGG
  AGGCCACCCATCAGACTGCGAAGATGGTGACCCTGCCGGAAGAGGACCCTGNAAGTGCTC
  ACCTTCCTCGNGCTCTTCATCTTCCTCACCTCCTTTCTTNCTGACTACTTCCACACCATG
  GTGGCCACCACCTGGTTCCCCAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_173344
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173344.1, NP_775479.1
• RefSeq Size: 2708 bp
• RefSeq ORF: 1023 bp
• Locus ID: 6482
• UniProt ID: Q11201
• Cytogenetics: 8q24.22
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - ganglio series, Glycosphingolipid biosynthesis - globo series, Keratan sulfate biosynthesis, Metabolic pathways, O-Glycan biosynthesis
• Gene Summary: The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein is normally found in the Golgi but can be proteolytically processed to a soluble form. Correct glycosylation of the encoded protein may be critical to its sialyltransferase activity. This protein, which is a member of glycosyltransferase family 29, can use the same acceptor substrates as does sialyltransferase 4B. Two transcript variants encoding the same protein have been found for this gene. Other transcript variants may exist, but have not been fully characterized yet. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.