Hexokinase Type III (HK3) (NM_002115) Human Untagged Clone

CAT#: SC110919

HK3 (untagged)-Human hexokinase 3 (white cell) (HK3), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Hexokinase Type III
• Synonyms: HKIII; HXK3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002115, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACTCCATTGGGTCTTCAGGGTTGCGGCAGGGGGAAGAAACCCTGAGTTGCTCTGAGGAGGGCTTGC
  CCGGGCCCTCAGACAGCTCAGAGCTGGTGCAGGAGTGCCTGCAGCAGTTCAAGGTGACAAGGGCACAGCT
  ACAGCAGATCCAAGCCAGCCTCTTGGGTTCCATGGAGCAGGCGCTGAGGGGACAGGCCAGCCCTGCCCCT
  GCGGTCCGGATGCTGCCTACATACGTGGGGTCCACCCCACATGGCACTGAGCAAGGAGACTTCGTGGTGC
  TGGAGCTGGGGGCCACAGGGGCCTCACTGCGTGTTTTGTGGGTGACTCTAACTGGCATTGAGGGGCATAG
  GGTGGAGCCCAGAAGCCAGGAGTTTGTGATCCCCCAAGAGGTGATGCTGGGTGCTGGCCAGCAGCTCTTT
  GACTTTGCTGCCCACTGCCTGTCTGAGTTCCTGGATGCGCAGCCTGTGAACAAACAGGGTCTGCAGCTTG
  GCTTCAGCTTCTCTTTCCCTTGTCACCAGACGGGCTTGGACAGGAGCACCCTCATTTCCTGGACCAAAGG
  TTTTAGGTGCAGTGGTGTGGAAGGCCAGGATGTGGTCCAGCTGCTGAGAGATGCCATTCGGAGGCAGGGG
  GCCTACAACATCGACGTGGTTGCTGTGGTGAACGACACAGTGGGCACCATGATGGGCTGTGAGCCGGGGG
  TCAGGCCGTGTGAGGTTGGGCTAGTTGTAGACACGGGCACCAACGCGTGTTACATGGAGGAGGCACGGCA
  TGTGGCAGTGCTGGACGAAGACCGGGGCCGCGTCTGCGTCAGCGTCGAGTGGGGCTCCTTCAGCGATGAT
  GGGGCGCTGGGACCAGTGCTGACCACCTTCGACCATACCCTGGACCATGAGTCCCTGAATCCTGGTGCTC
  AGAGGTTTGAGAAGATGATCGGAGGCCTGTACCTGGGTGAGCTGGTGCGGCTGGTGCTGGCTCACTTGGC
  CCGGTGTGGGGTCCTCTTTGGTGGCTGCACCTCCCCTGCCCTGCTGAGCCAAGGCAGCATCCTCCTGGAA
  CACGTGGCTGAGATGGAGGACCCCTCTACTGGGGCAGCCCGTGTCCATGCTATCCTGCAGGACTTGGGCC
  TGAGCCCTGGGGCTTCGGATGTTGAGCTTGTGCAGCACGTCTGTGCGGCCGTGTGCACGCGGGCTGCCCA
  GCTCTGTGCTGCCGCCCTGGCCGCTGTTCTCTCCTGCCTCCAGCACAGCCGGGAGCAACAAACACTCCAG
  GTTGCTGTGGCCACCGGAGGCCGAGTGTGTGAGCGGCACCCCAGGTTCTGCAGCGTCCTGCAGGGGACAG
  TGATGCTCCTGGCCCCGGAATGCGATGTCTCCTTAATCCCCTCTGTGGATGGTGGTGGCCGGGGAGTGGC
  GATGGTGACTGCCGTGGCTGCCCGTCTGGCTGCCCACCGGCGCCTGCTGGAGGAGACCCTGGCCCCATTC
  CGGTTGAACCATGATCAACTGGCTGCGGTTCAGGCACAGATGCGGAAGGCCATGGCCAAGGGGCTCCGAG
  GGGAGGCCTCCTCCCTTCGCATGCTGCCCACTTTCGTCCGGGCCACCCCTGACGGCAGCGAGCGAGGGGA
  TTTCCTGGCCCTGGACCTCGGGGGCACGAACTTCCGTGTCCTCCTGGTACGTGTGACCACAGGCGTGCAG
  ATCACCAGCGAGATCTACTCCATTCCCGAGACTGTGGCCCAGGGTTCTGGGCAGCAGCTCTTTGACCACA
  TCGTGGACTGCATCGTGGACTTCCAGCAGAAGCAGGGCCTGAGCGGGCAGAGCCTCCCACTGGGTTTTAC
  CTTCTCCTTCCCATGTAGGCAGCTTGGCCTAGACCAGGGCATCCTCCTGAACTGGACCAAGGGTTTCAAG
  GCATCAGACTGCGAGGGCCAAGATGTCGTGAGTCTGTTGCGGGAAGCCATCACTCGCAGACAGGCAGTGG
  AGCTGAATGTGGTTGCCATTGTCAATGACACGGTGGGGACCATGATGTCCTGTGGCTATGAGGACCCCCG
  TTGCGAGATAGGCCTCATTGTCGGAACCGGCACCAATGCCTGCTACATGGAGGAGCTCCGGAATGTGGCG
  GGCGTGCCTGGGGACTCAGGCCGCATGTGCATCAACATGGAGTGGGGCGCCTTTGGGGACGATGGCTCTC
  TGGCCATGCTCAGCACCCGCTTTGATGCAAGTGTGGACCAGGCGTCCATCAACCCCGGCAAGCAGAGGTT
  TGAAAAGATGATCAGCGGCATGTACCTGGGGGAGATCGTCCGCCACATCCTTTTACATTTAACCAGCCTT
  GGCGTTCTCTTCCGGGGCCAGCAGATCCAGCGCCTTCAGACCAGGGACATCTTCAAGACCAAGTTCCTCT
  CTGAGATCGAAAGTGACAGCCTGGCCCTGCGGCAGGTCCGAGCCATCCTAGAGGATCTGGGGCTACCCCT
  GACCTCAGATGACGCCCTGATGGTGCTAGAGGTGTGCCAGGCTGTGTCCCAGAGGGCTGCCCAGCTCTGT
  GGGGCGGGTGTAGCTGCCGTGGTGGAGAAGATCCGGGAGAACCGGGGCCTGGAAGAGCTGGCAGTGTCTG
  TGGGGGTGGATGGAACGCTCTACAAGCTGCACCCGCGCTTCTCCAGCCTGGTGGCGGCCACAGTGCGGGA
  GCTGGCCCCTCGCTGTGTGGTCACGTTCCTGCAGTCAGAGGATGGGTCCGGCAAAGGTGCGGCCCTGGTC
  ACCGCTGTTGCCTGCCGCCTTGCGCAGTTGACTCGTGTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002115 unedited
  AGGTTACTATTTGTATACGACTCATATAGGCGGCCCGCGAATTCGCACGAGGGGCCTCAT
  ATTGCCAGACAAGAGCTCAGACCTGAGGAGAGTGACTAGCTTCTCTGTGTCCCAGGTGGC
  CACCTTCCACTGTGGAAGCTCATGGACTCCATTGGGTCTTCAGGGTTGCGGCAGGGGGAA
  GAAACCCTGAGTTGCTCTGAGGAGGGCTTGCCCGGGCCCTCAGACAGCTCAGAGCTGGTG
  CAGGAGTGCCTGCAGCAGTTCAAGGTGACAAGGGCACAGCTACAGCAGATCCAAGCCAGC
  CTCTTGGGTTCCATGGAGCAGGCGCTGAGGGGACAGGCCAGCCCTGCCCCTGCGGTCCGG
  ATGCTGCCTACATACGTGGGGTCCACCCCACATGGCACTGAGCAAGGAGACTTCGTGGTG
  CTGGAGCTGGGGGCCACAGGGGCCTCACTGCGTGTTTTGTGGGTGACTCTAACTGGCATT
  GAGGGGCATAGGGTGGAGCCCAGAAGCCAGGAGTTTGTGATCCCCCAAGAGGTGATGCTG
  GGTGCTGGCCAGCAGCTCTTTGACTTTGCTGCCCACTGCCTGTCTGAGTTCCTGGATGCG
  CAGCCTGTGAACAAACAGGGTCTGCAGCTTGGCTTCAGCTTCTCTTTCCCTTGTCACCAG
  ACGGGCTTGGACAGGAGCACCCTCATTTCCTGGACCAAAGGTTTTAAGTGCAGTGGTGTG
  GAAGGCCAAGATGTGGTCCAACTGCTGAGAAGATGCCATTCGAGGCAGGGGGCCTACAAC
  ATCGACGTGGTTGCTGCGGTGAACGACACATGGGCACCATGATGGGCTGTGAGCCGGGGG
  CCAAGCCCCGTGAGTTGGGCTACTTGTACACCGGGCACCACGCCTGTTACATGGAGGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002115 unedited
  GCGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTATGGACACAGACCTTGCCCGGCCGCCGGGGCACATAGGGGTTTTATCGGGCGT
  GAATGTAAATAAATTATATATATATATTGCTAACCTGAGTGCTACTTTTCTCAGGGAAAG
  CCAGGGAGCAAGGCCAAATGGCCGCAAAGGGGTCACACATGGGATGTCCCAAGAGTCCTG
  GGTGGCTGGGCCTGGCTGGGAAACAGGCAAACCCCGACCCGGCTCCAGCAAGGCTGGGGG
  GGAAACCTCCTCAACCTGGAGGTTTCCTCAAACACGAGTCAACTGCGCAAGGCGGCAGGC
  AACAGCGGTGACCAGGGCCGCACCTTTGCCGGACCCATCCTTTGACTGCAGGAACGTGAC
  CACACAGCGAGGGGCCAGCTCCCGCACTGTGGCCGCCACCAGGCTGGAAAAGTCACCGCG
  GGTGCAGCTTGTAAAGCGTTCCATCCACCCCCACAGACACTGCCAGCTTTTCCAGGCCCC
  GGTTCTCCCGGATCTTCTCCACCACGGCAGCTACACCCGCCCCACAGAGCTGGGCAGCCC
  TCTGGGACACAGCCTGGCACACCTCTAGCACCATCAGGGCGTCATCTGAGAGTCAGGGGT
  AGCCCCAGATCCTCTAGGATGGCTCGGACCTGCCGCAGGGCCAGNCTGTCACTTTCGATC
  TCAGAGAGGAACTTGGTCTTGAAAATGTCCCTGGTCTGAAGCGCTGNATCTGCNTGCCCC
  CGNAAGAAACGCCNCAGCTGGTTAAATGTAAAAGATGTTGCGGCGATCTCCCCCAGTACA
  TGCCGCTGATATCTTTCAAACCTCCTGCTGCCGGGNTGATGGACCCCTGTTCCAACTTGC
  ATCAAAGNCGGGGCTGAGCATGGCCANAAACCATCGTCCCCAAAGCCCCCCCATTCATGT
  GATGCCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002115
• Insert Size: 3570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002115.1, NP_002106.1
• RefSeq Size: 3062 bp
• RefSeq ORF: 2772 bp
• Locus ID: 3101
• UniProt ID: P52790
• Cytogenetics: 5q35.2
• Domains: hexokinase
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus
• Gene Summary: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009]