FBXW7 (NM_033632) Human Untagged Clone

CAT#: SC110514

FBXW7 (untagged)-Human F-box and WD repeat domain containing 7 (FBXW7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FBXW7
• Synonyms: AGO; CDC4; FBW6; FBW7; FBX30; FBXO30; FBXW6; hAgo; hCdc4; SEL-10; SEL10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110514 sequence for NM_033632 edited (data generated by NextGen Sequencing)
  ATGAATCAGGAACTGCTCTCTGTGGGCAGCAAAAGACGACGAACTGGAGGCTCTCTGAGA
  GGTAACCCTTCCTCAAGCCAGGTAGATGAAGAACAGATGAATCGTGTGGTAGAGGAGGAA
  CAGCAACAGCAACTCAGACAACAAGAGGAGGAGCACACTGCAAGGAATGGTGAAGTTGTT
  GGAGTAGAACCTAGACCTGGAGGCCAAAATGATTCCCAGCAAGGACAGTTGGAAGAAAAC
  AATAATAGATTTATTTCGGTAGATGAGGACTCCTCAGGAAACCAAGAAGAACAAGAGGAA
  GATGAAGAACATGCTGGTGAACAAGATGAGGAGGATGAGGAGGAGGAGGAGATGGACCAG
  GAGAGTGACGATTTTGATCAGTCTGATGATAGTAGCAGAGAAGATGAACATACACATACT
  AACAGTGTCACGAACTCCAGTAGTATTGTGGACCTGCCCGTTCACCAACTCTCCTCCCCA
  TTCTATACAAAAACAACAAAAATGAAAAGAAAGTTGGACCATGGTTCTGAGGTCCGCTCT
  TTTTCTTTGGGAAAGAAACCATGCAAAGTCTCAGAATATACAAGTACCACTGGGCTTGTA
  CCATGTTCAGCAACACCAACAACTTTTGGGGACCTCAGAGCAGCCAATGGCCAAGGGCAA
  CAACGACGCCGAATTACATCTGTCCAGCCACCTACAGGCCTCCAGGAATGGCTAAAAATG
  TTTCAGAGCTGGAGTGGACCAGAGAAATTGCTTGCTTTAGATGAACTCATTGATAGTTGT
  GAACCAACACAAGTAAAACATATGATGCAAGTGATAGAACCCCAGTTTCAACGAGACTTC
  ATTTCATTGCTCCCTAAAGAGTTGGCACTCTATGTGCTTTCATTCCTGGAACCCAAAGAC
  CTGCTACAAGCAGCTCAGACATGTCGCTACTGGAGAATTTTGGCTGAAGACAACCTTCTC
  TGGAGAGAGAAATGCAAAGAAGAGGGGATTGATGAACCATTGCACATCAAGAGAAGAAAA
  GTAATAAAACCAGGTTTCATACACAGTCCATGGAAAAGTGCATACATCAGACAGCACAGA
  ATTGATACTAACTGGAGGCGAGGAGAACTCAAATCTCCTAAGGTGCTGAAAGGACATGAT
  GATCATGTGATCACATGCTTACAGTTTTGTGGTAACCGAATAGTTAGTGGTTCTGATGAC
  AACACTTTAAAAGTTTGGTCAGCAGTCACAGGCAAATGTCTGAGAACATTAGTGGGACAT
  ACAGGTGGAGTATGGTCATCACAAATGAGAGACAACATCATCATTAGTGGATCTACAGAT
  CGGACACTCAAAGTGTGGAATGCAGAGACTGGAGAATGTATACACACCTTATATGGGCAT
  ACTTCCACTGTGCGTTGTATGCATCTTCATGAAAAAAGAGTTGTTAGCGGTTCTCGAGAT
  GCCACTCTTAGGGTTTGGGATATTGAGACAGGCCAGTGTTTACATGTTTTGATGGGTCAT
  GTTGCAGCAGTCCGCTGTGTTCAATATGATGGCAGGAGGGTTGTTAGTGGAGCATATGAT
  TTTATGGTAAAGGTGTGGGATCCAGAGACTGAAACCTGTCTACACACGTTGCAGGGGCAT
  ACTAATAGAGTCTATTCATTACAGTTTGATGGTATCCATGTGGTGAGTGGATCTCTTGAT
  ACATCAATCCGTGTTTGGGATGTGGAGACAGGGAATTGCATTCACACGTTAACAGGGCAC
  CAGTCGTTAACAAGTGGAATGGAACTCAAAGACAATATTCTTGTCTCTGGGAATGCAGAT
  TCTACAGTTAAAATCTGGGATATCAAAACAGGACAGTGTTTACAAACATTGCAAGGTCCC
  AACAAGCATCAGAGTGCTGTGACCTGTTTACAGTTCAACAAGAACTTTGTAATTACCAGC
  TCAGATGATGGAACTGTAAAACTATGGGACTTGAAAACGGGTGAATTTATTCGAAACCTA
  GTCACATTGGAGAGTGGGGGGAGTGGGGGAGTTGTGTGGCGGATCAGAGCCTCAAACACA
  AAGCTGGTGTGTGCAGTTGGGAGTCGGAATGGGACTGAAGAAACCAAGCTGCTGGTGCTG
  GACTTTGATGTGGACATGAAGTGA
  
  Clone variation with respect to NM_033632.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033632 unedited
  TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGGAAACTTTACAAAAACAAA
  ATCCGGAGTCTCCCAAACCTGACTGTCCCGGGAGAAGTGGCCCTGGACGGGCAGAAGCCG
  CAGCCTGAAAAGACCCAGGAAGAGGAAAAGAGGAGTACCGCGCCGGAGCCTTCCGCAGCT
  GCCGCTTCAGTCCGAAGGAGGAAGGGAACCAACCCACTTTCTCGGCGCCGCGGCTCTTTT
  CTAAAAGTGATTACTTCCTTAGGATAGATTGCCAGAAGTGGAGTTACTGGGTCAGAGGAA
  TGTGAAAACCTTTGCATCTTCTGATAGTCTAGCCAAGGTCCAAGAAGTAGCAAGCTGGCT
  TTTGGAAATGAATCAGGAACTGCTCTCTGTGGGCAGCAAAAGACGACGAACTGGAGGCTC
  TCTGAGAGGTAACCCTTCCTCAAGCCAGGTAGATGAAGAACAGATGAATCGTGTGGTAGA
  GGAGGAACAGCAACAGCAACTCAGACAACAAGAGGAGGAGCACACTGCAAGGAATGGTGA
  AGTTGTTGGAGTAGAACCTAGACCTGGAGGCCAAAATGATTCCCAGCAAGGACAGTTGGA
  AGAAAACAATAATAGATTTATTTCGGTAGATGAGGACTCCTCAGGANACCAAGAAGAACA
  AGAGGAAGATGAAGAACATGCTGGTGAACAGAATGAGANGATGANGANGNNAGAGANATG
  GCCANGAGAGTGACNGATTTGATCAGTCTGATGATAGTAGCAGAGAAGATGAACATACAC
  ATACTAACAGTGTCACGAACTCCAGTAGTATTGTGGACCCTGCCCGTCACAACTNTNCTN
  NCCATTCTTACAAAACAACAAATGAAAGAAAGTGGACCATGGTNNCTGAGTCCGCTCTTT
  TTCTTGGAANGAANCATGCAAGTCTAGATATACAAGACACTGGGCTGTACCATGTCACAC
  ACACACTTTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033632 unedited
  NGTCATTGGAATGGCACTTCCATGCCAGGTAAAGCACTGGGGNAGGGGTCACAGGGATGC
  CACCCGGGATCTGTTCAAGAAACAGCTATGACCGCGGCCGCAATCTANAGTCGAGTTTTT
  TTTTTTTTTTTTTCTTTTTGCAGGGGGAAGGGCAGGGAGTATATCGTCTACACAATTGGA
  CAAATTCATCTTTTCTGCTCTTCACTTCATGTCCACATCAAAGTCCAGCACCAGCAGCTT
  GGTTTCTTCAGTCCCATTCCGACTCCCAACTGCACACACCAGCTTTGTGTTTGAGGCTCT
  GATCCGCCACACAACTCCCCCACTCCCCCCACTCTCCAATGTGACTAGGTTTCGAATAAA
  TTCACCCGTTTTCAAGTCCCATAGTTTTACAGTTCCATCATCTGAGCTGGTAATTACAAA
  GTTCTTGTTGAACTGTAAACAGGTCACAGCACTCTGATGCTTGTTGGGACCTTGCAATGT
  TTGTAAACACTGTCCTGTTTTGATATCCCAGATTTTAACTGTAGAATCTGCATTCCCAGA
  GACAAGAATATTGTCTTTGAGTTCCATTCCACTTGTTAACGACTGGTGCCCTGTTAACGT
  GTGAATGCAATTCCCTGTCTCCACATCCCAAACACGGATTGATGTATCAAGAGATCCACT
  CACCACTTGGAATACCCTCAAACTGTAATGAATAGACTCTATTAATATGCCCCTGCAACG
  TGTGTAAACAGGTTTCAATTCTCGGGATCCCCCACCTTTACCATAAAATCATATGCTCCA
  CTTAAAACCCTCCTGCCCTTTTATTGAACCCAGCGGAATGGTGCAACATGACCCATCAAA
  AAATGTAAACACTGGCCCTGGTCCAATATTCCAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033632
• Insert Size: 3000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033632.2, NP_361014.1
• RefSeq Size: 3896 bp
• RefSeq ORF: 2124 bp
• Locus ID: 55294
• UniProt ID: Q969H0
• Cytogenetics: 4q31.3
• Domains: WD40, F-box
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 5 encode the same isoform (1).