Ceramide synthase 2 (CERS2) (NM_013384) Human Untagged Clone

CAT#: SC110410

LASS2 (untagged)-Human LAG1 longevity assurance homolog 2 (S. cerevisiae) (LASS2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Ceramide synthase 2
• Synonyms: L3, SP260, TMSG1, MGC987, FLJ10243
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110410 sequence for NM_013384 edited (data generated by NextGen Sequencing)
  ATGGCCGTCATTGTGGATAAACCCTGGTTCTATGACATGAAGAAAGTTTGGGAGGGATAT
  CCCATACAGAGCACTATCCCTTCCCAGTATTGGTACTACATGATTGAACTTTCCTTCTAC
  TGGTCCCTGCTCTTCAGCATTGCCTCTGATGTCAAGCGAAAGGATTTCAAGGAACAGATC
  ATCCACCATGTGGCCACCATCATTCTCATCAGCTTTTCCTGGTTTGCCAATTACATCCGA
  GCTGGGACTCTAATCATGGCTCTGCATGACTCTTCCGATTACCTGCTGGAGTCAGCCAAG
  ATGTTTAACTACGCGGGATGGAAGAACACCTGCAACAACATCTTCATCGTCTTCGCCATT
  GTTTTTATCATCACCCGACTGGTCATCCTGCCCTTCTGGATCCTGCATTGCACCCTGGTG
  TACCCACTGGAGCTCTATCCTGCCTTCTTTGGCTATTACTTCTTCAATTCCATGATGGGA
  GTTCTACAGCTGCTGCATATCTTCTGGGCCTACCTCATTTTGCGCATGGCCCACAAGTTC
  ATAACTGGAAAGCTGGTAGAAGATGAACGCAGTGACCGGGAAGAAACAGAGAGCTCAGAG
  GGGGAGGAGGCTGCAGCTGGGGGAGGAGCAAAGAGCCGGCCCCTAGCCAATGGCCACCCC
  ATCCTCAATAACAACCATCGTAAGAATGACTGA
  
  Clone variation with respect to NM_013384.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013384 unedited
  CTCGGTTNGAAACGATTTATGGAGGCGGCAGACCCATTCCCACGAGGGCCCAGTCTTTCT
  CAGAGTTCCGAGAAGCCAGCTGGAGATTCACATTTTACCTGATTGCCTTCATTGCCGGCA
  TGGCCGTCATTGTGGATAAACCCTGGTTCTATGACATGTAACAAAGTTTGGGAGGGATAT
  CCCATACAGAGCACTATCCCTTCCCAGATGGGTACTACGTGATTGAACTTTCCTTCTACT
  GGTCCCTGCTCTTCAACATTGCCTCTGATGTCAAGCGAAAGGATTTTAAGGAACAGATCA
  TCCACCATGTGGCCACCATCATTCTCATCAGCTTTTCCTGGTTTGCCAATTACATCCGAG
  CTGGGACTCTAATCATGGCTCTGCATGACTCTTCCGATTACCTGCTGGAGTCAGCCAAGA
  TGTTTAACTACGCGGGATGGAAGAACACCTGCAACAACATCTTCATCGTCTTCGCCATTG
  TTTTTATCATCACCCGACTGGTCATCCTGCCCTTCTGGATCCTGCATTGCACCCTGGTGT
  ACCCACTGGAGCTCTATCCTGCCTTCTTTGGCTATTACTTCTTCAATTCCATGATGGGAG
  TTCTACAGCTGCTGCATATCTTCTGGGCCTACCTCATTTTGCGCATGGCCCACAAGTTCA
  TAACTGGAAAGCTGGTAGAAGATGAACGCAGTGACCGGGAAGAAACAGACAGCTCATAGG
  GGGATGAAGCTGCAGCTGGCGGAGGAGCATAGAGCCGGCCCCTAGCCAATGGCCACCCCA
  TCCTCAATAACACCCTCGTAGAATGACTGAACCATTATTCCCGCTGCCTCCCAGATATTG
  CATAAAGCCAGGAACTACCTGCTCCCTGGGCTAAGGGTCACTTTAAGCTCTGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013384 unedited
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTAAAAAAAAACTCTTTTCGTTTATTTAGTTGATCCCCCTTTTCAA
  TTCCTAATAGTCTGACCCTTATTGCCATGTTTTATTTAATATTTTTTATTTAATCTTTTA
  ATTTTAAAAAAAAACCCATTAACAGTACTTTTTGGTCTAAAAGGGCCCCTTTGCTGAAAT
  GCTAGGGGCTACCCGTAATTTTGGTTTTAAAACCAAAACCCCAAATTTTTAATAAATAAA
  AATTAAAATTAGTTGCCATTTTACTCCAAACCAGTTAGCCTAACTGAAAAAAAAAGGGAA
  GGGGGAAAAGGCCAAAAAAAGGAGGCAGTCAAATTTTAAACCTGTCGCTACAGGGACAGC
  TGAAAAAAGTACCCCTAAAAAAGATCTTCCGAGCAGTCCCCAGTACAGCCCCCATTTTTT
  GGCAAAGGTAGGGTAAGGGTTATGTGCACCCTCCTCCTACCCTCAATTAATTTGGGCCAT
  AAAGGGAGAAAGTTAAAAGCTCAGCTTTGGTTTCTGGCCCCAAGTTAGGGAGCTTAAAAA
  GGTTAGCCTTGGTCCAGCTTTTGCAGAATGAGGCCCACAGAGGGGACAATAAGGCCCAAC
  CCTGGCTCTGGAGGTCAGGNAGTCAAAGCCAAACAGTTGGGGCCCAAATTCTCAAAATAT
  GGGATGTGGATTTGTACCCCTTCCCACAGAGAGCTGGGGGAGGCCCTTCAAACCATAAAA
  AGATGATTGGTCCGGTTAGGAGGAAATACGACCGTCCCCCTTTACAGAAATAACACAGTC
  CCCTTACCTGGGGAAAGCCGGTTAAAATTTGTGTTAGAGCCCCTGCGGACACCCAGGAGG
  GAGGATGAAAAACTTTCCTTTATTTTCTCTTTCCCAAGATTTAAGGGCCCTTATCCCAGG
  AACAGGTTTTCTCTGCTTACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013384
• Insert Size: 1770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013384.3, NP_037516.3
• RefSeq Size: 2178 bp
• RefSeq ORF: 2178 bp
• Locus ID: 29956
• Cytogenetics: 1q21.3
• Domains: LAG1, TLC
• Protein Families: Transcription Factors, Transmembrane
• Gene Summary: This gene encodes a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter isoform (2) that has a shorter N-terminus compared to isoform 1.