EDEM3 (NM_025191) Human Untagged Clone

CAT#: SC110291

EDEM3 (untagged)-Human ER degradation enhancer, mannosidase alpha-like 3 (EDEM3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EDEM3
• Synonyms: C1orf22
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110291 sequence for NM_025191 edited (data generated by NextGen Sequencing)
  ATGAGCGAAGCCGGCGGCCGGGGCTGTGGGTCCCCGGTTCCCCAGCGAGCGCGATGGAGA
  CTAGTGGCGGCGACGGCCGCGTTCTGCCTGGTGTCGGCCACCTCCGTGTGGACGGCGGGG
  GCCGAGCCCATGAGTAGGGAGGAGAAACAGAAGCTTGGGAATCAAGTACTGGAAATGTTT
  GATCATGCTTATGGTAACTATATGGAACATGCTTACCCTGCTGATGAACTCATGCCTTTA
  ACCTGTAGAGGTCGAGTTAGAGGCCAAGAGCCAAGTCGCGGTGACGTTGATGATGCCTTG
  GGAAAATTTTCTCTGACACTGATTGATTCTTTGGACACTCTTGTGGTTTTAAATAAAACT
  AAAGAATTTGAAGATGCAGTGAGAAAAGTTTTAAGAGATGTTAATTTAGATAACGATGTA
  GTCGTATCAGTCTTTGAAACAAACATCAGAGTTCTTGGGGGTCTTTTGGGTGGGCACTCC
  CTGGCAATCATGCTGAAAGAAAAAGGTGAATATATGCAGTGGTACAATGATGAACTTCTC
  CAAATGGCAAAGCAGTTAGGTTACAAACTTTTACCGGCTTTCAACACTACCAGTGGCCTT
  CCTTATCCAAGAATTAATTTAAAGTTTGGCATCAGAAAACCAGAAGCTCGGACAGGAACT
  GAGACAGATACCTGTACAGCTTGTGCAGGTACCTTGATCCTTGAATTTGCTGCTTTAAGT
  CGATTCACAGGAGCAACAATATTTGAGGAATATGCCAGAAAAGCTCTTGATTTTCTCTGG
  GAAAAAAGACAGCGAAGTAGTAATTTAGTGGGCGTGACTATAAATATTCATACTGGAGAT
  TGGGTACGAAAAGATAGTGGAGTTGGAGCAGGGATTGATTCATATTATGAATATCTGTTG
  AAAGCCTATGTCTTGCTTGGAGATGACAGTTTTCTGGAAAGATTTAACACACACTATGAT
  GCCATAATGAGGTATATTAGCCAGCCACCTCTTCTACTTGATGTGCATATCCACAAACCA
  ATGCTGAATGCTCGGACTTGGATGGATGCTTTGCTTGCCTTCTTCCCAGGCTTGCAGGTG
  TTAAAGGGGGATATTAGACCTGCTATTGAAACTCATGAAATGTTATATCAGGTGATTAAA
  AAACACATTTTTCTACCAGAGGCATTTACCACAGATTTCAGAGTACACTGGGCTCAACAT
  CCTTTAAGGCCAGAATTTGCAGAAAGTACCTACTTCTTATATAAAGCTACAGGAGATCCT
  TACTACCTTGAAGTAGGGAAGACACTTATTGAAAATTTAAATAAATATGCTAGAGTGCCT
  TGCGGATTTGCTGCCATGAAGGATGTTCGTACTGGAAGTCATGAGGACAGAATGGATTCT
  TTCTTCTTGGCTGAAATGTTTAAATATCTTTACCTGTTATTTGCTGATAAAGAAGACATT
  ATTTTTGACATAGAAGATTACATCTTTACAACAGAAGCTCATCTGTTACCTCTTTGGCTC
  TCTACTACAAATCAAAGCATCTCTAAAAAGAACACAACCTCGGAATATACAGAACTGGAT
  GACAGTAACTTCGATTGGACTTGTCCAAATACTCAGATCCTCTTTCCTAATGACCCATTG
  TATGCTCAAAGTATTCGTGAGCCCTTGAAAAATGTGGTGGATAAGAGCTGTCCTAGAGGC
  ATCATCAGAGTAGAGGAGAGTTTCAGGAGTGGAGCTAAACCCCCTCTGAGAGCCAGAGAT
  TTCATGGCCACTAACCCTGAGCATTTAGAAATCCTGAAGAAGATGGGGGTGAGTTTGATT
  CACCTCAAAGATGGGAGAGTCCAGTTGGTCCAACATGCAATCCAAGCTGCTAGTTCAATC
  GACGCTGAAGATGGGTTGAGGTTCATGCAGGAGATGATTGAATTGTCAAGTCAGCAACAA
  AAAGAACAGCAGCTGCCTCCACGAGCTGTACAAATTGTTTCCCACCCATTTTTTGGCAGG
  GTAGTATTGACTGCTGGACCAGCTCAGTTTGGGCTGGATCTGTCTAAACATAAAGAGACA
  AGAGGATTTGTTGCAAGCAGTAAACCATCCAATGGTTGTTCAGAGCTTACTAACCCAGAG
  GCAGTGATGGGAAAAATCGCACTGATACAAAGAGGACAGTGCATGTTTGCAGAAAAGGCA
  CGCAACATCCAGAATGCTGGAGCCATTGGTGGCATTGTTATTGATGACAATGAGGGGAGC
  AGCAGTGATACTGCCCCTCTGTTCCAGATGGCAGGTGATGGAAAGGATACAGATGACATC
  AAGATCCCCATGCTGTTCTTATTCAGCAAAGAAGGAAGTATCATACTGGATGCCATCCGG
  GAATATGAGGAGGTAGAAGTGCTCCTCTCTGATAAAGCAAAAGATCGAGATCCTGAAATG
  GAAAATGAAGAACAACCATCCTCTGAAAATGATTCTCAGAATCAGAGTGGTGAACAGATT
  TCATCAAGTTCTCAGGAGGTTGATTTGGTTGATCAAGAGTCTTCTGAGGAAAATTCTCTA
  AATTCTCACCCAGAATCATTATCTCTAGCAGATATGGACAATGCTGCAAGCATTTCCCCT
  TCTGAACAGACTTCTAATCCCACAGAAAACCATGAGACTACAAATCTTAATGGTGAATGT
  ACAGATTTAGATAACCAGCTTCAAGAACAATCAGAAACTGAGGAAGATTCCAATCCTAAT
  GTTAGCTGGGGTAAAAAGGTCCAGCCTATAGACTCCATATTAGCAGACTGGAATGAAGAT
  ATAGAAGCATTTGAAATGATGGAGAAGGATGAGCTATGA
  
  Clone variation with respect to NM_025191.3
  1148 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025191 unedited
  GGTGTTCTAANATTTAGGTNATCACGNACTTACTTTTAGGGNCGGNNCGNGTATTTGGNA
  CGAGGGCCGGNGTGCCGTTCGTGTTACTCCGCATCCCGCCCCGTCTCGGCACGGCTAGCA
  GCCCCCTGGCCACCAGCGTCCAGCAATGTGTCTCACCGGCCGGGCGTAGCAGCTGCGCGT
  GCGCGGAACCGCGGGGCCATGAGCGAAGCCGGCGGCCGGGGCTGTGGGTCCCCGGTTCCC
  CAGCGAGCGCGATGGAGACTAGTGGCGGCGACGGCCGCGTTCTGCCTGGTGTCGGCCACC
  TCCGTGTGGACGGCGGGGGCCGAGCCCATGAGTAGGGAGGAGAAACAGAAGCTTGGGAAT
  CAAGTACTGGAAATGTTTGATCATGCTTATGGTAACTATATGGAACATGCTTACCCTGCT
  GATGAACTCATGCCTTTAACCTGTAGAGGTCGAGTTAGAGGCCAAGAGCCAAGTCGCGGC
  GACGTTGATGATGCCTTGGGAAAATTTTCTCTGACACCTGATTGATTCTTTGGACACTCT
  TGCGGTTTTAAATAACACTAAAGAATCTCGATATGCCCTGAGAAAAGTTTTAAGAGATGT
  TAATTTACATAACGATGCACTCGTATCAGTCCTTTGTAACCAACATTATTATTTCTTGGG
  GGTCTTTTTGGGTGGGCACTCCCTGCGCATTATGCTGAAAGAAAAGGTGTATATATGCAG
  CGGTACAATGATGAACTTTTTCCAAAGGGCAAGCAGTTTTGTTACAACTTTTTACCGGCT
  TTTAACATTACCAGTGGCCTTTCTTATCCACGATTAATTAAAAGTTTGCCTTAACAAACC
  ACAATCTTGGCCGTAACTCGAAAGATTCCTGTACAGCTGTGCAGAACCTTGATCCTTGAT
  TCGTTGTTTAATCTTTTTCAGGACCACCTATTTCGAGAACTTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_025191 unedited
  CGAGTGACCGAGGCCGCGCTCGAGTATACGAGTAAATCCCTTTTTTTTTTAAGGATTTAC
  TCCTCCTTAACAAGTGAACAATTTGCTTCTAACCGTCAATGAAAGGCAACACCTCCCTCT
  AATGGCCAAAGGAAGAGAGTGGCAGTAAGCTGGCTTTTCCAATGTGCCACACAATCCTTC
  ATGCCATTAACTTCTCCTTGCTGGAAAAGAAATCAGGTTGTCTCGATCACCTCACAAAAG
  TCACTCCTACACAACACCGACTTTCACCTACAAATACACCCTCCCATCCATGCCTATACC
  GCTCCCGCCCCCCATGCCCTACAGGCACCATCCGCTCATTCTCCTCCCGCCTCCCCCCCC
  TCTCCCACCGTCCGCCCTGTCTTTTATCTCCGACTATCCCGCCCCCCCCTCGCCTCCCCC
  TCCCCTCCCCCCCTTCTCCCCTCCCGCCTNTCCCCCCCCCCCCCCCCCCCCACCTCTCAT
  CCCTTCCTCTTCACCNCCTTCCCCCCTCTTCTCTCCCCCCTTCCCGCCCCCCCCCCCCTC
  CGCTCCCCCTCTCTCTTCCCCTCTCCTCCCTCTCCGCCCTCCCACCTCCCCCCGCGCCCT
  TCTCCTCCCCCCCTCCCCTCCCCACCCCTCCCCCCGCCCCTCCCTCTCCCCCCCCCCCCC
  CCGTCCTCTTCCCCCCCCCCACCCTCCCCCCCCCCCCCCCCCCCTCTTTCCCCCCCCCCC
  CCTTCCCTCCCCCCCTTCCCCCTCCCCTCCCCCCCTCCCCCCCCCCCCCCCCCACCCCTC
  ATCCCCCCCCCCCCCCCCCCCCCCTACTCCCTCTCCCCTTCCCCTACTCCTCGCCCCACC
  CCCTCCCCCCCCCCCTCACCCTCCCCCCACCCCCCCTCCCCACCTCCTCCACCCCCCCTC
  TCCCCCTTCCCTGCCCCCCCACGTCCTAACCCTCACCCCCCCCCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_025191
• Insert Size: 5410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025191.2, NP_079467.2
• RefSeq Size: 6298 bp
• RefSeq ORF: 2670 bp
• Locus ID: 80267
• UniProt ID: Q9BZQ6
• Cytogenetics: 1q25.3
• Domains: Glyco_hydro_47, PA
• Gene Summary: Quality control in the endoplasmic reticulum (ER) ensures that only properly folded proteins are retained in the cell through recognition and degradation of misfolded or unassembled proteins. EDEM3 belongs to a group of proteins that accelerate degradation of misfolded glycoproteins in the ER (Hirao et al., 2006 [PubMed 16431915]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.