EDEM3 (NM_025191) Human Tagged ORF Clone

CAT#: RC222885

EDEM3 (Myc-DDK-tagged)-Human ER degradation enhancer, mannosidase alpha-like 3 (EDEM3)

Product Images

Circular map for RC222885

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EDEM3
• Synonyms: C1orf22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC222885 representing NM_025191
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGAAGCCGGCGGCCGGGGCTGTGGGTCCCCGGTTCCCCAGCGAGCGCGATGGAGACTAGTGGCGG
  CGACGGCCGCGTTCTGCCTGGTGTCGGCCACCTCCGTGTGGACGGCGGGGGCCGAGCCCATGAGTAGGGA
  GGAGAAACAGAAGCTTGGGAATCAAGTACTGGAAATGTTTGATCATGCTTATGGTAACTATATGGAACAT
  GCTTACCCTGCTGATGAACTCATGCCTTTAACCTGTAGAGGTCGAGTTAGAGGCCAAGAGCCAAGTCGCG
  GTGACGTTGATGATGCCTTGGGAAAATTTTCTCTGACACTGATTGATTCTTTGGACACTCTTGTGGTTTT
  AAATAAAACTAAAGAATTTGAAGATGCAGTGAGAAAAGTTTTAAGAGATGTTAATTTAGATAACGATGTA
  GTCGTATCAGTCTTTGAAACAAACATCAGAGTTCTTGGGGGTCTTTTGGGTGGGCACTCCCTGGCAATCA
  TGCTGAAAGAAAAAGGTGAATATATGCAGTGGTACAATGATGAACTTCTCCAAATGGCAAAGCAGTTAGG
  TTACAAACTTTTACCGGCTTTCAACACTACCAGTGGCCTTCCTTATCCAAGAATTAATTTAAAGTTTGGC
  ATCAGAAAACCAGAAGCTCGGACAGGAACTGAGACAGATACCTGTACAGCTTGTGCAGGTACCTTGATCC
  TTGAATTTGCTGCTTTAAGTCGATTCACAGGAGCAACAATATTTGAGGAATATGCCAGAAAAGCTCTTGA
  TTTTCTCTGGGAAAAAAGACAGCGAAGTAGTAATTTAGTGGGCGTGACTATAAATATTCATACTGGAGAT
  TGGGTACGAAAAGATAGTGGAGTTGGAGCAGGGATTGATTCATATTATGAATATCTGTTGAAAGCCTATG
  TCTTGCTTGGAGATGACAGTTTTCTGGAAAGATTTAACACACACTATGATGCCATAATGAGGTATATTAG
  CCAGCCACCTCTTCTACTTGATGTGCATATCCACAAACCAATGCTGAATGCTCGGACTTGGATGGATGCT
  TTGCTTGCCTTCTTCCCAGGCTTGCAGGTGTTAAAGGGGGATATTAGACCTGCTATTGAAACTCATGAAA
  TGTTATATCAGGTGATTAAAAAACACAATTTTCTACCAGAGGCATTTACCACAGATTTCAGAGTACACTG
  GGCTCAACATCCTTTAAGGCCAGAATTTGCAGAAAGTACCTACTTCTTATATAAAGCTACAGGAGATCCT
  TACTACCTTGAAGTAGGGAAGACACTTATTGAAAATTTAAATAAATATGCTAGAGTGCCTTGCGGATTTG
  CTGCCATGAAGGATGTTCGTACTGGAAGTCATGAGGACAGAATGGATTCTTTCTTCTTGGCTGAAATGTT
  TAAATATCTTTACCTGTTATTTGCTGATAAAGAAGACATTATTTTTGACATAGAAGATTACATCTTTACA
  ACAGAAGCTCATCTGTTACCTCTTTGGCTCTCTACTACAAATCAAAGCATCTCTAAAAAGAACACAACCT
  CGGAATATACAGAACTGGATGACAGTAACTTCGATTGGACTTGTCCAAATACTCAGATCCTCTTTCCTAA
  TGACCCATTGTATGCTCAAAGTATTCGTGAGCCCTTGAAAAATGTGGTGGATAAGAGCTGTCCTAGAGGC
  ATCATCAGAGTAGAGGAGAGTTTCAGGAGTGGAGCTAAACCCCCTCTGAGAGCCAGAGATTTCATGGCCA
  CTAACCCTGAGCATTTAGAAATCCTGAAGAAGATGGGGGTGAGTTTGATTCACCTCAAAGATGGGAGAGT
  CCAGTTGGTCCAACATGCAATCCAAGCTGCTAGTTCAATCGACGCTGAAGATGGGTTGAGGTTCATGCAG
  GAGATGATTGAATTGTCAAGTCAGCAACAAAAAGAACAGCAGCTGCCTCCACGAGCTGTACAAATTGTTT
  CCCACCCATTTTTTGGCAGGGTAGTATTGACTGCTGGACCAGCTCAGTTTGGGCTGGATCTGTCTAAACA
  TAAAGAGACAAGAGGATTTGTTGCAAGCAGTAAACCATCCAATGGTTGTTCAGAGCTTACTAACCCAGAG
  GCAGTGATGGGAAAAATCGCACTGATACAAAGAGGACAGTGCATGTTTGCAGAAAAGGCACGCAACATCC
  AGAATGCTGGAGCCATTGGTGGCATTGTTATTGATGACAATGAGGGGAGCAGCAGTGATACTGCCCCTCT
  GTTCCAGATGGCAGGTGATGGAAAGGATACAGATGACATCAAGATCCCCATGCTGTTCTTATTCAGCAAA
  GAAGGAAGTATCATACTGGATGCCATCCGGGAATATGAGGAGGTAGAAGTGCTCCTCTCTGATAAAGCAA
  AAGATCGAGATCCTGAAATGGAAAATGAAGAACAACCATCCTCTGAAAATGATTCTCAGAATCAGAGTGG
  TGAACAGATTTCATCAAGTTCTCAGGAGGTTGATTTGGTTGATCAAGAGTCTTCTGAGGAAAATTCTCTA
  AATTCTCACCCAGAATCATTATCTCTAGCAGATATGGACAATGCTGCAAGCATTTCCCCTTCTGAACAGA
  CTTCTAATCCCACAGAAAACCATGAGACTACAAATCTTAATGGTGAATGTACAGATTTAGATAACCAGCT
  TCAAGAACAATCAGAAACTGAGGAAGATTCCAATCCTAATGTTAGCTGGGGTAAAAAGGTCCAGCCTATA
  GACTCCATATTAGCAGACTGGAATGAAGATATAGAAGCATTTGAAATGATGGAGAAGGATGAGCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC222885 representing NM_025191
  Red=Cloning site Green=Tags(s)
  
  MSEAGGRGCGSPVPQRARWRLVAATAAFCLVSATSVWTAGAEPMSREEKQKLGNQVLEMFDHAYGNYMEH
  AYPADELMPLTCRGRVRGQEPSRGDVDDALGKFSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDV
  VVSVFETNIRVLGGLLGGHSLAIMLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYPRINLKFG
  IRKPEARTGTETDTCTACAGTLILEFAALSRFTGATIFEEYARKALDFLWEKRQRSSNLVGVTINIHTGD
  WVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKPMLNARTWMDA
  LLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATGDP
  YYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIFT
  TEAHLLPLWLSTTNQSISKKNTTSEYTELDDSNFDWTCPNTQILFPNDPLYAQSIREPLKNVVDKSCPRG
  IIRVEESFRSGAKPPLRARDFMATNPEHLEILKKMGVSLIHLKDGRVQLVQHAIQAASSIDAEDGLRFMQ
  EMIELSSQQQKEQQLPPRAVQIVSHPFFGRVVLTAGPAQFGLDLSKHKETRGFVASSKPSNGCSELTNPE
  AVMGKIALIQRGQCMFAEKARNIQNAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDIKIPMLFLFSK
  EGSIILDAIREYEEVEVLLSDKAKDRDPEMENEEQPSSENDSQNQSGEQISSSSQEVDLVDQESSEENSL
  NSHPESLSLADMDNAASISPSEQTSNPTENHETTNLNGECTDLDNQLQEQSETEEDSNPNVSWGKKVQPI
  DSILADWNEDIEAFEMMEKDEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025191
• ORF Size: 2796 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025191.4
• RefSeq Size: 6670 bp
• RefSeq ORF: 2799 bp
• Locus ID: 80267
• UniProt ID: Q9BZQ6
• Cytogenetics: 1q25.3
• Domains: Glyco_hydro_47, PA
• MW: 105.1 kDa
• Gene Summary: Quality control in the endoplasmic reticulum (ER) ensures that only properly folded proteins are retained in the cell through recognition and degradation of misfolded or unassembled proteins. EDEM3 belongs to a group of proteins that accelerate degradation of misfolded glycoproteins in the ER (Hirao et al., 2006 [PubMed 16431915]).[supplied by OMIM, Mar 2008]