SOCS5 (NM_014011) Human Untagged Clone

CAT#: SC110087

SOCS5 (untagged)-Human suppressor of cytokine signaling 5 (SOCS5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SOCS5
• Synonyms: CIS6; Cish5; CISH6; SOCS-5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110087 sequence for NM_014011 edited (data generated by NextGen Sequencing)
  ATGGATAAAGTGGGAAAAATGTGGAATAACTTCAAATACAGGTGTCAGAATCTCTTCGGT
  CATGAGGGAGGAAGCCGTAGTGAAAATGTGGACATGAACTCCAACAGATGTTTGTCTGTC
  AAAGAGAAAAACATCAGCATAGGAGACTCAACTCCTCAGCAACAAAGCAGTCCCTTAAGA
  GAAAATATTGCCTTACAACTGGGATTAAGCCCTTCGAAGAATTCTTCAAGGAGAAATCAA
  AATTGTGCCACAGAAATCCCTCAAATTGTTGAAATAAGCATCGAAAAGGATAATGATTCT
  TGTGTTACCCCAGGAACAAGACTTGCACGAAGAGATTCCTACTCTCGACATGCTCCATGG
  GGTGGGAAGAAAAAACATTCCTGTTCTACAAAGACCCAGAGTTCATTGGATGCTGATAAA
  AAGTTTGGTAGAACTCGAAGTGGACTTCAAAGGAGAGAGAGGCGCTACGGCGTAAGTTCT
  GTACACGACATGGACAGTGTTTCCAGCAGAACTGTAGGAAGTCGCTCTCTAAGACAGAGG
  TTGCAGGATACTGTGGGCTTGTGTTTTCCCATGAGAACTTACAGCAAGCAGTCAAAGCCT
  CTCTTTTCCAATAAAAGAAAAATCCATCTCTCTGAATTAATGCTTGAGAAATGCCCTTTT
  CCTGCTGGCTCAGATTTAGCCCAAAAATGGCATTTGATTAAACAGCATACAGCTCCTGTG
  AGCCCACATTCAACATTTTTTGATACATTTGATCCATCTTTGGTTTCTACAGAAGATGAA
  GAAGATAGGCTTAGAGAGAGAAGGCGGCTTAGTATTGAAGAAGGGGTTGATCCCCCTCCC
  AATGCACAAATACATACATTTGAAGCTACTGCACAGGTTAATCCATTATATAAACTGGGA
  CCAAAATTAGCTCCTGGAATGACTGAAATAAGTGGGGACAGTTCTGCAATTCCACAAGCT
  AATTGTGACTCGGAAGAGGATACAACCACCCTGTGTTTGCAGTCACGGAGGCAGAAGCAG
  CGTCAGATATCTGGAGACAGCCATACGCATGTTAGCAGACAGGGAGCTTGGAAAGTCCAC
  ACACAGATTGATTACATACACTGCCTCGTGCCTGATTTGCTTCAAATTACAGGGAATCCC
  TGTTACTGGGGAGTGATGGACCGTTATGAAGCAGAAGCCCTTCTCGAAGGGAAACCTGAA
  GGCACGTTTTTGCTCAGGGACTCTGCGCAAGAGGACTACCTCTTCTCTGTGAGCTTCCGC
  CGCTACAACAGATCCCTGCATGCCCGAATTGAGCAGTGGAATCACAACTTTAGTTTCGAC
  GCCCATGACCCGTGTGTATTTCACTCCTCCACTGTAACGGGACTTTTAGAACATTATAAA
  GATCCCAGTTCGTGCATGTTTTTTGAACCATTGCTTACTATATCACTAAATAGGACTTTC
  CCTTTTAGCCTGCAGTATATCTGTCGCGCGGTAATCTGCAGGTGCACTACGTATGATGGA
  ATTGATGGGCTCCCTCTACCCTCAATGTTACAGGATTTTTTAAAAGAGTATCATTATAAA
  CAAAAAGTTAGAGTTCGCTGGTTGGAACGAGAACCAGTCAAGGCAAAGTAA
  
  Clone variation with respect to NM_014011.4
  1047 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014011 unedited
  NGTTCAGATTTGTATACGATGACTATAGGCGGCCCGCGAATTCGCACGAGGCCTCGTGCC
  GAATTCGGCACGAGGGACGCACCGGAGGGCAGGCGGACTCGCCCTGTCGGTGACTGCGCC
  GTCCGGGCCCGTCCTGCCTGGCCGCAGGTGCCCTGGATGAGGCCGCCCCGCGCGCCCCAA
  ACGATTTTATAATCAATGGATAAAGTGTGTTATATGTGGAATAACTTCAAATACAGGTGT
  CAGAATCTCTTCGGTCATGAGGGAGGAAGCCGTAGTGAAAATGTGGACATGAACTCCAAC
  AGATGTTTGTCTGTCAAAGAGAAAAACATCAGCATAGGAGACTCAACTCCTCAGCAACAA
  AGCAGTCCCTTAAGAGAAAATATTGCCTTACAACTGGGATTAAGCCCTTCGAAGAATTCT
  TCAAGGAGAAATCAAAATTGTGCCACAGAAATCCCTCAAATTGTTGAAATAAGCATCGAA
  AAGGATAATGATTCTTGTGTTACCCCAGGAACAAGACTTGCACGAAGAGATTCCTACTCT
  CGACATGCTCCATGGGGTGGGAAGAAAAAACATTCCTGTTCTACAAAGACCCAGAGTTCA
  TTGGATGCTGATAAAAAGTTTGATAGAACTCGAAGTGGACTTCAAAGGAGAGAGACGCGC
  TACCGCGTAAGTTCTGTACACGACATGGACAGTGTTTCCCGCAGAACTGTANGAAGTCGC
  TCTCTAACACAGAGTTGCACGATACCGCGGGCTTGCGCTTCTCCACTGAGACTTACAGAA
  GCAGTCACAAGCCTTCTTTTTCAATAAAAGAAAACCCTCTCTCTGAATTAAGCTTGAAAA
  AGCCCCTATACTGCTGGCTTAAATTAGCCCAAAATGGCCTTTGGTTAAACACATACCGCT
  CC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014011 unedited
  NNNNTTTAGCTTTGAACCGGCGGCCGCATNCTAAGATCGAGTTTTTTTTTTTTTTTTTTG
  CAAGTTCATGCTGGTGATTTTTCTTTTTTAAAAGTAAGCCACTAATATTTTAAGACAAAA
  ATTTAAACCAAAAATTGCATATTAATATTATACACAAACTGAAACATGTGACTTTTAGCA
  CAGTGTATACATGCTTACATAAACTCAAACACATTTAAAACAATTTCCATTTAACATATG
  AAAGTTTTGAAATACATAAAAGCATTAATACTTTCTATAAAACTACAGTTAGAACATCAA
  TTTGCTACAAGCATATAAAAACATCAAGTGCTTTCTTGGAGTTTCAAGTTTCCTCTCTAA
  AACACAATCTTGCTCACATATATAGTCCTTTTCCAATAATCAGTATGAACTTTTCTTTTT
  CTCAAGAGGTTTATTTTCCAGGTACATTATAAGCTCAAGAAAAAAGACCCAACAATTAGG
  CAATATAATTATGTCATAACAACTTAATTGCACATAATGCTTTAAAGTAGGTTTGGATGA
  AGTTCTCAAACACTTTGGTATATGATCTTCAGGGACTTTAATTAGAAACAACAGAAATTA
  AAAGTCCCTTCAATCCCCATTTTGCTTTAAAAGGGACAAGTCTTTGGTTAAAGTTCTACA
  TTTTGGGCATTTATTTTTAATTAAGATGGTCCCCAAACCAATAAATGGTACTGATCTATT
  ACAAATTCACAAGACTTCATTTAACCTGCTTCTGATGCAGTGGGAGGTTACAGATACTCA
  TTTACTATAATATGTCATTGGATAGTTTCAATCTTTCATTTATAAGCAGAGAAATCAATA
  NGGTTATTACTTCTCTGTCAGTTTTCTGTTATAACCTAAACTGTACANGTATTTGCAGTA
  ACTACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014011
• Insert Size: 4760 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014011.4, NP_054730.1
• RefSeq Size: 4422 bp
• RefSeq ORF: 1611 bp
• Locus ID: 9655
• UniProt ID: O75159
• Cytogenetics: 2p21
• Domains: SH2, SOCS
• Protein Families: Druggable Genome
• Protein Pathways: Jak-STAT signaling pathway
• Gene Summary: The protein encoded by this gene contains a SH2 domain and a SOCS BOX domain. The protein thus belongs to the suppressor of cytokine signaling (SOCS) family, also known as STAT-induced STAT inhibitor (SSI) protein family. SOCS family members are known to be cytokine-inducible negative regulators of cytokine signaling. The specific function of this protein has not yet been determined. Two alternatively spliced transcript variants encoding an identical protein have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Both variants encode the same protein.