AKAP12 (NM_144497) Human Untagged Clone

CAT#: SC110079

AKAP12 (untagged)-Human A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AKAP12
• Synonyms: AKAP250; SSeCKS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110079 sequence for NM_144497 edited (data generated by NextGen Sequencing)
  ATGCTGGGGACCATCACCATCACAGTTGGACAGAGAGACTCTGAAGATGTGAGCAAAAGA
  GACTCCGATAAAGAGATGGCTACTAAGTCAGCGGTTGTTCACGACATCACAGATGATGGG
  CAGGAGGAGACACCCGAAATAATCGAACAGATTCCTTCTTCAGAAAGCAATTTAGAAGAG
  CTAACACAACCCACTGAGTCCCAGGCTAATGATATTGGATTTAAGAAGGTGTTTAAGTTT
  GTTGGCTTTAAATTCACTGTGAAAAAGGATAAGACAGAGAAGCCTGACACTGTCCAGCTA
  CTCACTGTGAAGAAAGATGAAGGGGAGGGAGCAGCAGGGGCTGGCGACCACAAGGACCCC
  AGCCTTGGGGCTGGAGAAGCAGCATCCAAAGAAAGCGAACCCAAACAATCTACAGAGAAA
  CCCGAAGAGACCCTGAAGCGTGAGCAAAGCCACGCAGAAATTTCTCCCCCAGCCGAATCT
  GGCCAAGCAGTGGAGGAATGCAAAGAGGAAGGAGAAGAGAAACAAGAAAAAGAACCTAGC
  AAGTCTGCAGAATCTCCGACTAGTCCCGTGACCAGTGAAACAGGATCAACCTTCAAAAAA
  TTCTTCACTCAAGGTTGGGCCGGCTGGCGCAAAAAGACCAGTTTCAGGAAGCCGAAGGAG
  GATGAAGTGGAAGCTTCAGAGAAGAAAAAGGAACAAGAGCCAGAAAAAGTAGACACAGAA
  GAAGACGGAAAGGCAGAGGTTGCCTCCGAGAAACTGACCGCCTCCGAGCAAGCCCACCCA
  CAGGAGCCGGCAGAAAGTGCCCACGAGCCCCGGTTATCAGCTGAATATGAGAAAGTTGAG
  CTGCCCTCAGAGGAGCAAGTCAGTGGCTCGCAGGGACCTTCTGAAGAGAAACCTGCTCCG
  TTGGCGACAGAAGTGTTTGATGAGAAAATAGAAGTCCACCAAGAAGAGGTTGTGGCCGAA
  GTCCACGTCAGCACCGTGGAGGAGAGAACCGAAGAGCAGAAAACGGAGGTGGAAGAAACA
  GCAGGGTCTGTGCCAGCTGAAGAATTGGTTGAAATGGATGCAGAACCTCAGGAAGCTGAA
  CCTGCCAAGGAGCTGGTGAAGCTCAAAGAAACGTGTGTTTCCGGAGAGGACCCTACACAG
  GGAGCTGACCTCAGTCCTGATGAGAAGGTGCTGTCCAAACCCCCCGAAGGCGTTGTGAGT
  GAGGTGGAAATGCTGTCATCACAGGAGAGAATGAAGGTGCAGGGAAGTCCACTAAAGAAG
  CTTTTTACCAGCACTGGCTTAAAAAAGCTTTCTGGAAAGAAACAGAAAGGGAAAAGAGGA
  GGAGGAGACGAGGAATCAGGGGAGCACACTCAGGTTCCAGCCGATTCTCCGGACAGCCAG
  GAGGAGCAAAAGGGCGAGAGCTCTGCCTCATCCCCTGAGGAGCCCGAGGAGATCACGTGT
  CTGGAAAAGGGCTTAGCCGAGGTGCAGCAGGATGGGGAAGCTGAAGAAGGAGCTACTTCC
  GATGGAGAGAAAAAAAGAGAAGGTGTCACTCCCTGGGCATCATTCAAAAAGATGGTGACG
  CCCAAGAAGCGTGTTAGACGGCCTTCGGAAAGTGATAAAGAAGATGAGCTGGACAAGGTC
  AAGAGCGCTACCTTGTCTTCCACCGAGAGCACAGCCTCTGAAATGCAAGAAGAAATGAAA
  GGGAGCGTGGAAGAGCCAAAGCCGGAAGAACCAAAGCGCAAGGTGGATACCTCAGTATCT
  TGGGAAGCTTTAATTTGTGTGGGATCATCCAAGAAAAGAGCAAGGAGAGGGTCCTCTTCT
  GATGAGGAAGGGGGACCAAAAGCAATGGGAGGAGACCACCAGAAAGCTGATGAGGCCGGA
  AAAGACAAAGAGACGGGGACAGACGGGATCCTTGCTGGTTCCCAAGAACATGATCCAGGG
  CAGGGAAGTTCCTCCCCGGAGCAAGCTGGAAGCCCTACCGAAGGGGAGGGCGTTTCCACC
  TGGGAGTCATTTAAAAGGTTAGTCACGCCAAGAAAAAAATCAAAGTCCAAGCTGGAAGAG
  AAAAGCGAAGACTCCATAGCTGGGTCTGGTGTAGAACATTCCACTCCAGACACTGAACCC
  GGTAAAGAAGAATCCTGGGTCTCAATCAAGAAGTTTATTCCTGGACGAAGGAAGAAAAGG
  CCAGATGGGAAACAAGAACAAGCCCCTGTTGAAGACGCAGGGCCAACAGGGGCCAACGAA
  GATGACTCTGATGTCCCGGCCGTGGTCCCTCTGTCTGAGTATGATGCTGTAGAAAGGGAG
  AAAATGGAGGCACAGCAAGCCCAAAAAAGCGCAGAGCAGCCCGAGCAGAAGGCAGCCACT
  GAGGTGTCCAAGGAGCTCAGCGAGAGTCAGGTTCATATGATGGCAGCAGCTGTCGCTGAC
  GGGACGAGGGCAGCTACCATTATTGAAGAAAGGTCTCCTTCTTGGATATCTGCTTCAGTG
  ACAGAACCTCTTGAACAAGTAGAAGCTGAAGCCGCACTGTTAACTGAGGAGGTATTGGAA
  AGAGAAGTAATTGCAGAAGAAGAACCCCCCACGGTTACTGAACCTCTGCCAGAGAACAGA
  GAGGCCCGGGGCGACACGGTCGTTAGTGAGGCGGAATTGACCCCCGAAGCTGTGACAGCT
  GCAGAAACTGCAGGGCCATTGGGTGCCGAAGAAGGAACCGAAGCATCTGCTGCTGAAGAG
  ACCACAGAAATGGTGTCAGCAGTCTCCCAGTTAACCGACTCCCCAGACACCACAGAGGAG
  GCCACTCCGGTGCAGGAGGTGGAAGGTGGCGTACCTGACATAGAAGAGCAAGAGAGGCGG
  ACTCAAGAGGTCCTCCAGGCAGTGGCAGAAAAAGTGAAAGAGGAATCCCAGCTGCCTGGC
  ACCGGTGGGCCAGAAGATGTGCTTCAGCCTGTGCAGAGAGCAGAGGCAGAAAGACCAGAA
  GAGCAGGCTGAAGCGTCGGGTCTGAAGAAAGAGACGGATGTAGTGTTGAAAGTAGATGCT
  CAGGAGGCAAAAACTGAGCCTTTTACACAAGGGAAGGTGGTGGGGCAGACCACCCCAGAA
  AGCTTTGAAAAAGCTCCTCAAGTCACAGAGAGCATAGAGTCCAGTGAGCTTGTAACCACT
  TGTCAAGCCGAAACCTTAGCTGGGGTAAAATCACAGGAGATGGTGATGGAACAGGCTATC
  CCCCCTGACTCGGTGGAAACCCCTACAGACAGTGAGACTGATGGAAGCACCCCCGTAGCC
  GACTTTGACGCACCAGGCACAACCCAGAAAGACGAGATTGTGGAAATCCATGAGGAGAAT
  GAGGTCGCATCTGGTACCCAGTCAGGGGGCACAGAAGCAGAGGCAGTTCCTGCACAGAAA
  GAGAGGCCTCCAGCACCTTCCAGTTTTGTGTTCCAGGAAGAAACTAAAGAACAATCAAAG
  ATGGAAGACACTCTAGAGCATACAGATAAAGAGGTGTCAGTGGAAACTGTATCCATTCTG
  TCAAAGACTGAGGGGACTCAAGAGGCTGACCAGTATGCTGATGAGAAAACCAAAGACGTA
  CCATTTTTCGAAGGACTTGAGGGGTCTATAGACACAGGCATAACAGTCAGTCGGGAAAAG
  GTCACTGAAGTTGCCCTTAAAGGTGAAGGGACAGAAGAAGCTGAATGTAAAAAGGATGAT
  GCTCTTGAACTGCAGAGTCACGCTAAGTCTCCTCCATCCCCCGTGGAGAGAGAGATGGTA
  GTTCAAGTCGAAAGGGAGAAAACAGAAGCAGAGCCAACCCATGTGAATGAAGAGAAGCTT
  GAGCACGAAACAGCTGTTACCGTATCTGAAGAGGTCAGTAAGCAGCTCCTCCAGACAGTG
  AATGTGCCCATCATAGATGGGGCAAAGGAAGTCAGCAGTTTGGAAGGAAGCCCTCCTCCC
  TGCCTAGGTCAAGAGGAGGCAGTATGCACCAAAATTCAAGTTCAGAGCTCTGAGGCATCA
  TTCACTCTAACAGCGGCTGCAGAGGAGGAAAAGGTCTTAGGAGAAACTGCCAACATTTTA
  GAAACAGGTGAAACGTTGGAGCCTGCAGGTGCACATTTAGTTCTGGAAGAGAAATCCTCT
  GAAAAAAATGAAGACTTTGCCGCTCATCCAGGGGAAGATGCTGTGCCCACAGGGCCCGAC
  TGTCAGGCAAAATCGACACCAGTGATAGTATCTGCTACTACCAAGAAAGGCTTAAGTTCG
  GACCTGGAAGGAGAGAAAACCACATCACTGAAGTGGAAGTCAGATGAAGTCGATGAGCAG
  GTTGCTTGCCAGGAGGTCAAAGTGAGTGTAGCAATTGAGGATTTAGAGCCTGAAAATGGG
  ATTTTGGAACTTGAGACCAAAAGCAGTAAACTTGTCCAAAACATCATCCAGACAGCCGTT
  GACCAGTTTGTACGTACAGAAGAAACAGCCACCGAAATGTTGACGTCTGAGTTACAGACA
  CAAGCTCACGTGATAAAAGCTGACAGCCAGGACGCTGGACAGGAAACGGAGAAAGAAGGA
  GAGGAACCTCAGGCCTCTGCACAGGATGAAACACCAATTACTTCAGCCAAAGAGGAGTCA
  GAGTCAACCGCAGTGGGACAAGCACATTCTGATATTTCCAAAGACATGAGTGAAGCCTCA
  GAAAAGACCATGACTGTTGAGGTAGAAGGTTCCACTGTAAATGATCAGCAGCTGGAAGAG
  GTCGTCCTCCCATCTGAGGAAGAGGGAGGTGGAGCTGGAACAAAGTCTGTGCCAGAAGAT
  GATGGTCATGCCTTGTTAGCAGAAAGAATAGAGAAGTCACTAGTTGAACCGAAAGAAGAT
  GAAAAAGGTGATGATGTTGATGACCCTGAAAACCAGAACTCAGCCCTGGCTGATACTGAT
  GCCTCAGGAGGCTTAACCAAAGAGTCCCCAGATACAAATGGACCAAAACAAAAAGAGAAG
  GAGGATGCCCAGGAAGTAGAATTGCAGGAAGGAAAAGTGCACAGTGAATCAGATAAAGCG
  ATCACACCCCAAGCACAGGAGGAGTTACAGAAACAAGAGAGAGAATCTGCAAAGTCAGAA
  CTTACAGAATCTTAA
  
  Clone variation with respect to NM_144497.2
  4200 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_144497 unedited
  GCGGCCGGCGAATTCGCACGAGGGTCTGGGCGCTCAGTCCGCTCTGATCCCGCCGAACCA
  CCTGCGGTTGGCAGGCAGGAGACTAGGCGTCTGCCGGGGAGGGCAGGGACCCGCTAAGCT
  GATCTCCTGTACAGTAGTGCTACTTAAAATATGCTGGGGACCATCACCATCACAGTTGGA
  CAGAGAGACTCTGAAGATGTGAGCAAAAGAGACTCCGATAAAGAGATGGCTACTAAGTCA
  GCGGTTGTTCACGACATCACAGATGATGGGCAGGAGGAGACACCCGAAATAATCGAACAG
  ATTCCTTCTTCAGAAAGCAATTTAGAAGAGCTAACACAACCCACTGAGTCCCAGGCTAAT
  GATATTGGATTTAAGAAGGTGTTTAAGTTTGTTGGCTTTAAATTCACTGTGAAAAAGGAT
  AAGACAGAGAAGCCTGACACTGTCCAGCTACTCACTGTGAAGAAAGATGAAGGGGAGGGA
  GCAGCAGGGGCTGGCGACCACAAGGACCCCAGCCTTGGGGCTGGAGAAGCAGCATCCAAA
  GAAAGCGAACCCAAACAATCTACAGAGAAACCCGAAGAGACCCTGAAGCGTGAGCAAAGC
  CACGCAGAAATTTCTCCCCCAGCCGAATCTGGCCAAGCAGTGGAGGAATGCAAAGAGGAA
  GGAGAAGAGAAACAAGAANAAGAAACCTAGCAGTCTGCAGAATCTCCGACTAGTCCCGTG
  ACCAGTGANACAGGATCAACCTTCANAAAATTCTTCACTCAAGGNTGGGCCNGNCTGGCG
  CAAAAAGACCAGTTTCAGGAANCCCGAGGAGGATNNGAATGGAAGCTTCANAGAAGAAAA
  GGGAACANGAGCCNGNANNAGTNNACACAGAAAAGACGGAAAGGCCANAGGTTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_144497 unedited
  TTTACGGAATTCTATGNNACCGCGGCCGCAATCTANNATCGAGTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTAAGTGAAGCAAATATTTTATTTAAATCAGTCGTCAAATCACAACTTATC
  CAAATGAATGTGTTGCAACATTGTTCTTAAAAGAATGGCACAAATATGGCACAAACACAA
  TCCAGTATCTATCAAAATACCATTTGTAAAGAACCTGACTTATTTCATGCCATGTGTGAA
  TGCAGTCCAGCATATAAGAGTAGAATCAAAAACTTACAACAATTCTTTTCTTCATAACAT
  ATAAAACATATTCACTACTTTGCCTCAGGATTAAGCACACTTATCGGTCCAAACAACCAT
  AACAAGTAGGAAAAAGGATGTAAAGTTAATTTGCTACCAGAATATAACTTCTTTCTAACC
  TCCACTGGGTTAGAAAGAAAAATTTCCCAATGACAATAGAACATTCTGTATGTTTCAAAA
  TGCTTTTTAGTTTTAGCACACTCCGTGTAATAGGAAAGTGACCAATTTCAGAGTACCACT
  AAATATGGACCTCAATCATTAGTGAATTTTAAAGAATTGTACCTTGATCAGGAATGTTAC
  ATCTAGGAGGCTCTGTTTTTCGTGGGTTATATAACTGAAGCTTAAAGCTCCCCAAGCCTG
  CACTATGGCACATGCCCCATCATATACTGTGTGATACAAAAAACATGTAGTATCCTGCAC
  ATTTCCCTTAACATTAAGAAAAAAATATTCCATAGATACCGGAGGACTACTTTAAACATA
  CATATAAATATGTAAAGGATGATACCTCCAGTTTCAGAAACAGAACTAGGTATTGCCCAA
  CTCCATTTTCAGGTTAAAAATTTTAGGACCCTTAATCAGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_144497
• Insert Size: 6000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144497.1, NP_653080.1
• RefSeq Size: 6287 bp
• RefSeq ORF: 5055 bp
• Locus ID: 9590
• UniProt ID: Q02952
• Cytogenetics: 6q25.1
• Domains: PkinA_anch
• Protein Families: Druggable Genome
• Gene Summary: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) has an alternate 5' segment, compared to variant 1, that results in a distinct 5' UTR and 5' coding region. The resulting isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.