AKAP12 (NM_144497) Human Tagged ORF Clone

CAT#: RG214439

AKAP12 (tGFP-tagged) - Human A kinase (PRKA) anchor protein 12 (AKAP12), transcript variant 2

Product Images

Circular map for RG214439

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AKAP12
• Synonyms: AKAP250; SSeCKS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214439 representing NM_144497
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGGACCATCACCATCACAGTTGGACAGAGAGACTCTGAAGATGTGAGCAAAAGAGACTCCGATA
  AAGAGATGGCTACTAAGTCAGCGGTTGTTCACGACATCACAGATGATGGGCAGGAGGAGACACCCGAAAT
  AATCGAACAGATTCCTTCTTCAGAAAGCAATTTAGAAGAGCTAACACAACCCACTGAGTCCCAGGCTAAT
  GATATTGGATTTAAGAAGGTGTTTAAGTTTGTTGGCTTTAAATTCACTGTGAAAAAGGATAAGACAGAGA
  AGCCTGACACTGTCCAGCTACTCACTGTGAAGAAAGATGAAGGGGAGGGAGCAGCAGGGGCTGGCGACCA
  CAAGGACCCCAGCCTTGGGGCTGGAGAAGCAGCATCCAAAGAAAGCGAACCCAAACAATCTACAGAGAAA
  CCCGAAGAGACCCTGAAGCGTGAGCAAAGCCACGCAGAAATTTCTCCCCCAGCCGAATCTGGCCAAGCAG
  TGGAGGAATGCAAAGAGGAAGGAGAAGAGAAACAAGAAAAAGAACCTAGCAAGTCTGCAGAATCTCCGAC
  TAGTCCCGTGACCAGTGAAACAGGATCAACCTTCAAAAAATTCTTCACTCAAGGTTGGGCCGGCTGGCGC
  AAAAAGACCAGTTTCAGGAAGCCGAAGGAGGATGAAGTGGAAGCTTCAGAGAAGAAAAAGGAACAAGAGC
  CAGAAAAAGTAGACACAGAAGAAGACGGAAAGGCAGAGGTTGCCTCCGAGAAACTGACCGCCTCCGAGCA
  AGCCCACCCACAGGAGCCGGCAGAAAGTGCCCACGAGCCCCGGTTATCAGCTGAATATGAGAAAGTTGAG
  CTGCCCTCAGAGGAGCAAGTCAGTGGCTCGCAGGGACCTTCTGAAGAGAAACCTGCTCCGTTGGCGACAG
  AAGTGTTTGATGAGAAAATAGAAGTCCACCAAGAAGAGGTTGTGGCCGAAGTCCACGTCAGCACCGTGGA
  GGAGAGAACCGAAGAGCAGAAAACGGAGGTGGAAGAAACAGCAGGGTCTGTGCCAGCTGAAGAATTGGTT
  GAAATGGATGCAGAACCTCAGGAAGCTGAACCTGCCAAGGAGCTGGTGAAGCTCAAAGAAACGTGTGTTT
  CCGGAGAGGACCCTACACAGGGAGCTGACCTCAGTCCTGATGAGAAGGTGCTGTCCAAACCCCCCGAAGG
  CGTTGTGAGTGAGGTGGAAATGCTGTCATCACAGGAGAGAATGAAGGTGCAGGGAAGTCCACTAAAGAAG
  CTTTTTACCAGCACTGGCTTAAAAAAGCTTTCTGGAAAGAAACAGAAAGGGAAAAGAGGAGGAGGAGACG
  AGGAATCAGGGGAGCACACTCAGGTTCCAGCCGATTCTCCGGACAGCCAGGAGGAGCAAAAGGGCGAGAG
  CTCTGCCTCATCCCCTGAGGAGCCCGAGGAGATCACGTGTCTGGAAAAGGGCTTAGCCGAGGTGCAGCAG
  GATGGGGAAGCTGAAGAAGGAGCTACTTCCGATGGAGAGAAAAAAAGAGAAGGTGTCACTCCCTGGGCAT
  CATTCAAAAAGATGGTGACGCCCAAGAAGCGTGTTAGACGGCCTTCGGAAAGTGATAAAGAAGATGAGCT
  GGACAAGGTCAAGAGCGCTACCTTGTCTTCCACCGAGAGCACAGCCTCTGAAATGCAAGAAGAAATGAAA
  GGGAGCGTGGAAGAGCCAAAGCCGGAAGAACCAAAGCGCAAGGTGGATACCTCAGTATCTTGGGAAGCTT
  TAATTTGTGTGGGATCATCCAAGAAAAGAGCAAGGAGAGGGTCCTCTTCTGATGAGGAAGGGGGACCAAA
  AGCAATGGGAGGAGACCACCAGAAAGCTGATGAGGCCGGAAAAGACAAAGAGACGGGGACAGACGGGATC
  CTTGCTGGTTCCCAAGAACATGATCCAGGGCAGGGAAGTTCCTCCCCGGAGCAAGCTGGAAGCCCTACCG
  AAGGGGAGGGCGTTTCCACCTGGGAGTCATTTAAAAGGTTAGTCACGCCAAGAAAAAAATCAAAGTCCAA
  GCTGGAAGAGAAAAGCGAAGACTCCATAGCTGGGTCTGGTGTAGAACATTCCACTCCAGACACTGAACCC
  GGTAAAGAAGAATCCTGGGTCTCAATCAAGAAGTTTATTCCTGGACGAAGGAAGAAAAGGCCAGATGGGA
  AACAAGAACAAGCCCCTGTTGAAGACGCAGGGCCAACAGGGGCCAACGAAGATGACTCTGATGTCCCGGC
  CGTGGTCCCTCTGTCTGAGTATGATGCTGTAGAAAGGGAGAAAATGGAGGCACAGCAAGCCCAAAAAAGC
  GCAGAGCAGCCCGAGCAGAAGGCAGCCACTGAGGTGTCCAAGGAGCTCAGCGAGAGTCAGGTTCATATGA
  TGGCAGCAGCTGTCGCTGACGGGACGAGGGCAGCTACCATTATTGAAGAAAGGTCTCCTTCTTGGATATC
  TGCTTCAGTGACAGAACCTCTTGAACAAGTAGAAGCTGAAGCCGCACTGTTAACTGAGGAGGTATTGGAA
  AGAGAAGTAATTGCAGAAGAAGAACCCCCCACGGTTACTGAACCTCTGCCAGAGAACAGAGAGGCCCGGG
  GCGACACGGTCGTTAGTGAGGCGGAATTGACCCCCGAAGCTGTGACAGCTGCAGAAACTGCAGGGCCATT
  GGGTGCCGAAGAAGGAACCGAAGCATCTGCTGCTGAAGAGACCACAGAAATGGTGTCAGCAGTCTCCCAG
  TTAACCGACTCCCCAGACACCACAGAGGAGGCCACTCCGGTGCAGGAGGTGGAAGGTGGCGTACCTGACA
  TAGAAGAGCAAGAGAGGCGGACTCAAGAGGTCCTCCAGGCAGTGGCAGAAAAAGTGAAAGAGGAATCCCA
  GCTGCCTGGCACCGGTGGGCCAGAAGATGTGCTTCAGCCTGTGCAGAGAGCAGAGGCAGAAAGACCAGAA
  GAGCAGGCTGAAGCGTCGGGTCTGAAGAAAGAGACGGATGTAGTGTTGAAAGTAGATGCTCAGGAGGCAA
  AAACTGAGCCTTTTACACAAGGGAAGGTGGTGGGGCAGACCACCCCAGAAAGCTTTGAAAAAGCTCCTCA
  AGTCACAGAGAGCATAGAGTCCAGTGAGCTTGTAACCACTTGTCAAGCCGAAACCTTAGCTGGGGTAAAA
  TCACAGGAGATGGTGATGGAACAGGCTATCCCCCCTGACTCGGTGGAAACCCCTACAGACAGTGAGACTG
  ATGGAAGCACCCCCGTAGCCGACTTTGACGCACCAGGCACAACCCAGAAAGACGAGATTGTGGAAATCCA
  TGAGGAGAATGAGGTCGCATCTGGTACCCAGTCAGGGGGCACAGAAGCAGAGGCAGTTCCTGCACAGAAA
  GAGAGGCCTCCAGCACCTTCCAGTTTTGTGTTCCAGGAAGAAACTAAAGAACAATCAAAGATGGAAGACA
  CTCTAGAGCATACAGATAAAGAGGTGTCAGTGGAAACTGTATCCATTCTGTCAAAGACTGAGGGGACTCA
  AGAGGCTGACCAGTATGCTGATGAGAAAACCAAAGACGTACCATTTTTCGAAGGACTTGAGGGGTCTATA
  GACACAGGCATAACAGTCAGTCGGGAAAAGGTCACTGAAGTTGCCCTTAAAGGTGAAGGGACAGAAGAAG
  CTGAATGTAAAAAGGATGATGCTCTTGAACTGCAGAGTCACGCTAAGTCTCCTCCATCCCCCGTGGAGAG
  AGAGATGGTAGTTCAAGTCGAAAGGGAGAAAACAGAAGCAGAGCCAACCCATGTGAATGAAGAGAAGCTT
  GAGCACGAAACAGCTGTTACCGTATCTGAAGAGGTCAGTAAGCAGCTCCTCCAGACAGTGAATGTGCCCA
  TCATAGATGGGGCAAAGGAAGTCAGCAGTTTGGAAGGAAGCCCTCCTCCCTGCCTAGGTCAAGAGGAGGC
  AGTATGCACCAAAATTCAAGTTCAGAGCTCTGAGGCATCATTCACTCTAACAGCGGCTGCAGAGGAGGAA
  AAGGTCTTAGGAGAAACTGCCAACATTTTAGAAACAGGTGAAACGTTGGAGCCTGCAGGTGCACATTTAG
  TTCTGGAAGAGAAATCCTCTGAAAAAAATGAAGACTTTGCCGCTCATCCAGGGGAAGATGCTGTGCCCAC
  AGGGCCCGACTGTCAGGCAAAATCGACACCAGTGATAGTATCTGCTACTACCAAGAAAGGCTTAAGTTCC
  GACCTGGAAGGAGAGAAAACCACATCACTGAAGTGGAAGTCAGATGAAGTCGATGAGCAGGTTGCTTGCC
  AGGAGGTCAAAGTGAGTGTAGCAATTGAGGATTTAGAGCCTGAAAATGGGATTTTGGAACTTGAGACCAA
  AAGCAGTAAACTTGTCCAAAACATCATCCAGACAGCCGTTGACCAGTTTGTACGTACAGAAGAAACAGCC
  ACCGAAATGTTGACGTCTGAGTTACAGACACAAGCTCACGTGATAAAAGCTGACAGCCAGGACGCTGGAC
  AGGAAACGGAGAAAGAAGGAGAGGAACCTCAGGCCTCTGCACAGGATGAAACACCAATTACTTCAGCCAA
  AGAGGAGTCAGAGTCAACCGCAGTGGGACAAGCACATTCTGATATTTCCAAAGACATGAGTGAAGCCTCA
  GAAAAGACCATGACTGTTGAGGTAGAAGGTTCCACTGTAAATGATCAGCAGCTGGAAGAGGTCGTCCTCC
  CATCTGAGGAAGAGGGAGGTGGAGCTGGAACAAAGTCTGTGCCAGAAGATGATGGTCATGCCTTGTTAGC
  AGAAAGAATAGAGAAGTCACTAGTTGAACCGAAAGAAGATGAAAAAGGTGATGATGTTGATGACCCTGAA
  AACCAGAACTCAGCCCTGGCTGATACTGATGCCTCAGGAGGCTTAACCAAAGAGTCCCCAGATACAAATG
  GACCAAAACAAAAAGAGAAGGAGGATGCCCAGGAAGTAGAATTGCAGGAAGGAAAAGTGCACAGTGAATC
  AGATAAAGCGATCACACCCCAAGCACAGGAGGAGTTACAGAAACAAGAGAGAGAATCTGCAAAGTCAGAA
  CTTACAGAATCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214439 representing NM_144497
  Red=Cloning site Green=Tags(s)
  
  MLGTITITVGQRDSEDVSKRDSDKEMATKSAVVHDITDDGQEETPEIIEQIPSSESNLEELTQPTESQAN
  DIGFKKVFKFVGFKFTVKKDKTEKPDTVQLLTVKKDEGEGAAGAGDHKDPSLGAGEAASKESEPKQSTEK
  PEETLKREQSHAEISPPAESGQAVEECKEEGEEKQEKEPSKSAESPTSPVTSETGSTFKKFFTQGWAGWR
  KKTSFRKPKEDEVEASEKKKEQEPEKVDTEEDGKAEVASEKLTASEQAHPQEPAESAHEPRLSAEYEKVE
  LPSEEQVSGSQGPSEEKPAPLATEVFDEKIEVHQEEVVAEVHVSTVEERTEEQKTEVEETAGSVPAEELV
  EMDAEPQEAEPAKELVKLKETCVSGEDPTQGADLSPDEKVLSKPPEGVVSEVEMLSSQERMKVQGSPLKK
  LFTSTGLKKLSGKKQKGKRGGGDEESGEHTQVPADSPDSQEEQKGESSASSPEEPEEITCLEKGLAEVQQ
  DGEAEEGATSDGEKKREGVTPWASFKKMVTPKKRVRRPSESDKEDELDKVKSATLSSTESTASEMQEEMK
  GSVEEPKPEEPKRKVDTSVSWEALICVGSSKKRARRGSSSDEEGGPKAMGGDHQKADEAGKDKETGTDGI
  LAGSQEHDPGQGSSSPEQAGSPTEGEGVSTWESFKRLVTPRKKSKSKLEEKSEDSIAGSGVEHSTPDTEP
  GKEESWVSIKKFIPGRRKKRPDGKQEQAPVEDAGPTGANEDDSDVPAVVPLSEYDAVEREKMEAQQAQKS
  AEQPEQKAATEVSKELSESQVHMMAAAVADGTRAATIIEERSPSWISASVTEPLEQVEAEAALLTEEVLE
  REVIAEEEPPTVTEPLPENREARGDTVVSEAELTPEAVTAAETAGPLGAEEGTEASAAEETTEMVSAVSQ
  LTDSPDTTEEATPVQEVEGGVPDIEEQERRTQEVLQAVAEKVKEESQLPGTGGPEDVLQPVQRAEAERPE
  EQAEASGLKKETDVVLKVDAQEAKTEPFTQGKVVGQTTPESFEKAPQVTESIESSELVTTCQAETLAGVK
  SQEMVMEQAIPPDSVETPTDSETDGSTPVADFDAPGTTQKDEIVEIHEENEVASGTQSGGTEAEAVPAQK
  ERPPAPSSFVFQEETKEQSKMEDTLEHTDKEVSVETVSILSKTEGTQEADQYADEKTKDVPFFEGLEGSI
  DTGITVSREKVTEVALKGEGTEEAECKKDDALELQSHAKSPPSPVEREMVVQVEREKTEAEPTHVNEEKL
  EHETAVTVSEEVSKQLLQTVNVPIIDGAKEVSSLEGSPPPCLGQEEAVCTKIQVQSSEASFTLTAAAEEE
  KVLGETANILETGETLEPAGAHLVLEEKSSEKNEDFAAHPGEDAVPTGPDCQAKSTPVIVSATTKKGLSS
  DLEGEKTTSLKWKSDEVDEQVACQEVKVSVAIEDLEPENGILELETKSSKLVQNIIQTAVDQFVRTEETA
  TEMLTSELQTQAHVIKADSQDAGQETEKEGEEPQASAQDETPITSAKEESESTAVGQAHSDISKDMSEAS
  EKTMTVEVEGSTVNDQQLEEVVLPSEEEGGGAGTKSVPEDDGHALLAERIEKSLVEPKEDEKGDDVDDPE
  NQNSALADTDASGGLTKESPDTNGPKQKEKEDAQEVELQEGKVHSESDKAITPQAQEELQKQERESAKSE
  LTES
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144497
• ORF Size: 5052 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144497.2
• RefSeq Size: 6287 bp
• RefSeq ORF: 5055 bp
• Locus ID: 9590
• UniProt ID: Q02952
• Cytogenetics: 6q25.1
• Domains: PkinA_anch
• Protein Families: Druggable Genome
• Gene Summary: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]