DR5 (TNFRSF10B) (NM_147187) Human Untagged Clone

CAT#: SC109989

TNFRSF10B (untagged)-Human tumor necrosis factor receptor superfamily, member 10b (TNFRSF10B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TNFRSF10B
• Synonyms: CD262; DR5; KILLER; KILLER/DR5; TRAIL-R2; TRAILR2; TRICK2; TRICK2A; TRICK2B; TRICKB; ZTNFR9
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_147187 edited
  ATAAATCAGCACGCGGCCGGAGAACCCCGCAATCTCTGCGCCCACAAAATACACCGACGA
  TGCCCGATCTACTTTAAGGGCTGAAACCCACGGGCCTGAGAGACTATAAGAGCGTTCCCT
  ACCGCCATGGAACAACGGGGACAGAACGCCCCGGCCGCTTCGGGGGCCCGGAAAAGGCAC
  GGCCCAGGACCCAGGGAGGCGCGGGGAGCCAGGCCTGGGCTCCGGGTCCCCAAGACCCTT
  GTGCTCGTTGTCGCCGCGGTCCTGCTGTTGGTCTCAGCTGAGTCTGCTCTGATCACCCAA
  CAAGACCTAGCTCCCCAGCAGAGAGCGGCCCCACAACAAAAGAGGTCCAGCCCCTCAGAG
  GGATTGTGTCCACCTGGACACCATATCTCAGAAGACGGTAGAGATTGCATCTCCTGCAAA
  TATGGACAGGACTATAGCACTCACTGGAATGACCTCCTTTTCTGCTTGCGCTGCACCAGG
  TGTGATTCAGGTGAAGTGGAGCTAAGTCCCTGCACCACGACCAGAAACACAGTGTGTCAG
  TGCGAAGAAGGCACCTTCCGGGAAGAAGATTCTCCTGAGATGTGCCGGAAGTGCCGCACA
  GGGTGTCCCAGAGGGATGGTCAAGGTCGGTGATTGTACACCCTGGAGTGACATCGAATGT
  GTCCACAAAGAATCAGGCATCATCATAGGAGTCACAGTTGCAGCCGTAGTCTTGATTGTG
  GCTGTGTTTGTTTGCAAGTCTTTACTGTGGAAGAAAGTCCTTCCTTACCTGAAAGGCATC
  TGCTCAGGTGGTGGTGGGGACCCTGAGCGTGTGGACAGAAGCTCACAACGACCTGGGGCT
  GAGGACAATGTCCTCAATGAGATCGTGAGTATCTTGCAGCCCACCCAGGTCCCTGAGCAG
  GAAATGGAAGTCCAGGAGCCAGCAGAGCCAACAGGTGTCAACATGTTGTCCCCCGGGGAG
  TCAGAGCATCTGCTGGAACCGGCAGAAGCTGAAAGGTCTCAGAGGAGGAGGCTGCTGGTT
  CCAGCAAATGAAGGTGATCCCACTGAGACTCTGAGACAGTGCTTCGATGACTTTGCAGAC
  TTGGTGCCCTTTGACTCCTGGGAGCCGCTCATGAGGAAGTTGGGCCTCATGGACAATGAG
  ATAAAGGTGGCTAAAGCTGAGGCAGCGGGCCACAGGGACACCTTGTACACGATGCTGATA
  AAGTGGGTCAACAAAACCGGGCGAGATGCCTCTGTCCACACCCTGCTGGATGCCTTGGAG
  ACGCTGGGAGAGAGACTTGCCAAGCAGAAGATTGAGGACCACTTGTTGAGCTCTGGAAAG
  TTCATGTATCTAGAAGGTAATGCAGACTCTGCCATGTCCTAAGTGTGATTCTCTTCAGGA
  AGTCAGACCTTCCCTGGTTTACCTTTTTTCTGGAAAAAGCCCAACTGGACTCCAGTCAGT
  AGGAAAGTGCCACAATTGTCACATGACCGGTACTGGAAGAAACTCTCCCATCCAACATCA
  CCCAGTGGATGGAACATCCTGTAACTTTTCACTGCACTTGGCATTATTTTTATAAGCTGA
  ATGTGATAATAAGGACACTATGGAAATGTCTGGATCATTCCGTTTGTGCGTACTTTGAGA
  TTTGGTTTGGGATGTCATTGTTTTCACAGCACTTTTTTATCCTAATGTAAATGCTTTATT
  TATTTATTTGGGCTACATTGTAAGATCCATCTACACAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_147187 unedited
  ACTATAGGCGGCCGCGNAATTCGCACGAGGATAAATCAGCACGCGGCCGGAGAACCCCGC
  AATCTCTGCGCCCACAAAATACACCGACGATGCCCGATCTACTTTAAGGGCTGAAACCCA
  CGGGCCTGAGAGACTATAAGAGCGTTCCCTACCGCCATGGAACAACGGGGACAGAACGCC
  CCGGCCGCTTCGGGGGCCCGGAAAAGGCACGGCCCAGGACCCAGGGAGGCGCGGGGAGCC
  AGGCCTGGGCTCCGGGTCCCCAAGACCCTTGTGCTCGTTGTCGCCGCGGTCCTGCTGTTG
  GTCTCAGCTGAGTCTGCTCTGATCACCCAACAAGACCTAGCTCCCCAGCAGAGAGCGGCC
  CCACAACAAAAGAGGTCCAGCCCCTCAGAGGGATTGTGTCCACCTGGACACCATATCTCA
  GAAGACGGTAGAGATTGCATCTCCTGCAAATATGGACAGGACTATAGCACTCACTGGAAT
  GACCTCCTTTTCTGCTTGCGCTGCACCAGGTGTGATTCAGGTGAAGTGGAGCTAAGTCCC
  TGCACCACGACCAGAAACACAGTGGTGTCAGTGCGAAGAAGGCACCTTCCGGGAAGAAGA
  ATTCTCTGAGATGTGCCGGGAAGTGCCGCACAGGCTGTCCCCAGAGGGATGGTCAAGGGT
  CNGGTGATGGTACACCCTGGCATGACATCCNAATGTGTCCACAAAGAATCAGGCATCATC
  ATAGGAGTCACAGTTGCANNCGTAGTCTTGATNGGGGGCTTGGGTTTTGTTGGCAAGTCT
  TTCCTGGGGAAAAAAAGGCCTTCCTTACCTGAAGGCATTGCTCAGGGGGGGGGGGGGGGG
  GGACCCTGAGCGTGTGGACAGAAACTAACAAGACTGGGGGGCTGGGGACAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_147187 unedited
  TTGGACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTGTGTAGATGG
  ATCTTACAATGTAGCCCAAATAAATAAATAAAGCATTTACATTAGGATAAAAAAGTGCTG
  TGAAAACAATGACATCCCAAACCAAATCTCAAAGTACGCACAAACGGAATGATCCAGACA
  TTTCCATAGTGTCCTTATTATCACATTCAGCTTATAAAAATAATGCCAAGTGCAGTGAAA
  AGTTACAGGATGTTCCATCCACTGGGTGATGTTGGATGGGAGAGTTTCTTCCAGTACCGG
  TCATGTGACAATTGTGGCACTTTCCTACTGACTGGAGTCCAGTTGGGCTTTTTCCAGAAA
  AAAGGTAAACCAGGGAAGGTCTGACTTCCTGAAGAGAATCACACTTAGGACATGGCAGAG
  TCTGCATTACCTTCTAGATACATGAACTTTCCAGAGCTCAACAAGTGGTCCTCAATCTTC
  TGCTTGGCAAGTCTCTCTCCCAGCGTCTCCAAGGCATCCAGCAGGGTGTGGACAGAGGCA
  TCTCGCCCGGTTTTGTTGACCCACTTTATCAGCATCGTGTACAAGGTGTCCCTGTGGCCC
  GCTGCCTCAGCTTTAGCCACCTTTATCTCATTGTCCATGAGGCCCAACTTCCTCATGAGC
  GGCTCCCAGGAGTCAAAGGGCACCAAGTCTGCAAAGTCATCGAAGCACTGTCTCAGAGTC
  TCAGTGGGATCACCTTCATTTGCTGGAACCAGCAGCCTCCTCCTCTGAGACCTTTCAGCT
  TCTGCCGGNTCCAGCAGATGCTCCTGACTCCCGGGGGACACATGNTGACACCTGTTGGCT
  CTGCTGGCTNCTGGACTTCCATTNTCTGCTCAAGGACCTGGGTGGGGCTGCAGAACTCAC
  GATCTCATTGAGGACATTGNCTTCAGCCCCNAGTCGTTGTGAGCTCTGTCCCACGCTCAG
  GNTCCCCACACCACTGAGCAGAGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_147187
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_147187.1, NP_671716.1
• RefSeq Size: 4073 bp
• RefSeq ORF: 1236 bp
• Locus ID: 8795
• UniProt ID: O14763
• Cytogenetics: 8p21.3
• Domains: DEATH, TNFR
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Apoptosis, Cytokine-cytokine receptor interaction, Natural killer cell mediated cytotoxicity, p53 signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (2) lacks an in-frame coding segment compared to variant 1. The resulting isoform (2) lacks an internal region, as compared to isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.