PPAP2C (PLPP2) (NM_177526) Human Untagged Clone

CAT#: SC109964

PPAP2C (untagged)-Human phosphatidic acid phosphatase type 2C (PPAP2C), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPAP2C
• Synonyms: LPP2; PAP-2c; PAP2-g; PPAP2C
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109964 sequence for NM_177526 edited (data generated by NextGen Sequencing)
  ATGGCTGGGGTCACCATCACGGCCACCGTCATCCTTGTCTCGGCCGGGGAAGCCTACCTG
  GTGTACACAGACCGGCTCTATTCTCGCTCGGACTTCAACAACTACGTGGCTGCTGTATAC
  AAGGTGCTGGGGACCTTCCTGTTTGGGGCTGCCGTGAGCCAGTCTCTGACAGACCTGGCC
  AAGTACATGATTGGGCGTCTGAGGCCCAACTTCCTAGCCGTCTGCGACCCCGACTGGAGC
  CGGGTCAACTGCTCGGTCTATGTGCAGCTGGAGAAGGTGTGCAGGGGAAACCCTGCTGAT
  GTCACCGAGGCCAGGTTGTCTTTCTACTCGGGACACTCTTCCTTTGGGATGTACTGCATG
  GTGTTCTTGGCGCTGTATGTGCAGGCACGACTCTGTTGGAAGTGGGCACGGCTGCTGCGA
  CCCACAGTCCAGTTCTTCCTGGTGGCCTTTGCCCTCTACGTGGGCTACACCCGCGTGTCT
  GATTACAAACACCACTGGAGCGATGTCCTTGTTGGCCTCCTGCAGGGGGCACTGGTGGCT
  GCCCTCACTGTCTGCTACATCTCAGACTTCTTCAAAGCCCGACCCCCACAGCACTGTCTG
  AAGGAGGAGGAGCTGGAACGGAAGCCCAGCCTGTCACTGACGTTGACCCTGGGCGAGGCT
  GACCACAACCACTATGGATACCCGCACTCCTCCTCCTGA
  
  Clone variation with respect to NM_177526.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_177526 unedited
  TTTGTATACGACTCACTATAGGCGGCACGCGAATTCGCACCAGGCGACGGGACGCGCTGG
  GACCGGCGTCGGGGGTCGCGGGGACCATGCAGCGGAGCCTCCCTGCCCTTCGCTATCCTG
  ACGCTGGTGAACGCCCCGTACAAGCGAGGATTTTACTGCGGGGATGACTCCATCCGGTAC
  CCCTACCGTCCATATACCATCACCCACGGGCTCATGGCTGGGGTCACCATCACGGCCACC
  GTCATCCTTGTCTCGGCCGGGGAAGCCTACCTGGTGTACACAGACCGGCTCTATTCTCGC
  TCGGACTTCAACAACTACGTGGCTGCTGTATACAAGGTGCTGGGGACCTTCCTGTTTGGG
  GCTGCCGTGAGCCAGTCTCTGACAGACCTGGCCAAGTACATGATTGGGCGTCTGATGCCC
  AACTTCCTATCCGTCTGCGACCCCGACTGGAGCCGGGTCAACTGCTCGGTCTATGTGCAG
  CTGGAGAATGTGTGCAGGGGAAACCCTGCTGATGTCACCGATGCCAGGTTGTCTTTCTAC
  TCGGGACACTCTTCCTTTGGGATGTACTGCATGGTGTTCTTGGCGCTGTATGTGCATGCA
  CGACTCTGTTGGATGTGGGCACGGCTGCTGCGACCCACAGTCCAGTTCTTCCTGGTGGCC
  TTTGCCCTCTACGTGGGCTACACCCGCGTGTCTGATTACAACACCACTGGAGCGATGTCC
  TTGGTGGNCTCCTGCAGGGAGCACTGGTGGCTGCCCTCACTGTCTGCTACATCTCAAACT
  TCTTTCAAGCCCGACCCCCACAGCACTGTCTGATGGATGATGATCTGGAACGGATGCCAT
  NCTGTCACTGACGTTGACCCTGGGCGATGCTGACCACATCACTATGATACCCGACTTCTC
  TNCTGAGGCCGACCCGNCCAGCAGGAGCTCTGTGAGTCACTGAGCCCACCAGTGGTCCTC
  ANCCTGGTAGCACTGAGCTCTGACGGCTCAGAACCTGGCTGATGGACATGACGGCTCCGT
  CCCCTGCTGACTGACNAATCGGATCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_177526
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177526.1, NP_803545.1
• RefSeq Size: 1286 bp
• RefSeq ORF: 699 bp
• Locus ID: 8612
• UniProt ID: O43688
• Cytogenetics: 19p13.3
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Sphingolipid metabolism
• Gene Summary: The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is similar to phosphatidic acid phosphatase type 2A (PPAP2A) and type 2B (PPAP2B). All three proteins contain 6 transmembrane regions, and a consensus N-glycosylation site. This protein has been shown to possess membrane associated PAP activity. Three alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) has an alternate 5' terminal exon, which results in a downstream translation start codon, as compared to variant 3. The resulting isoform (2) has a shorter N-terminus, as compared to isoform 3.