ROBO1 (NM_002941) Human Untagged Clone

CAT#: SC109739

ROBO1 (untagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ROBO1
• Synonyms: DUTT1; SAX3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002941 edited
  GAATTCGGCACGAGGCCCGACTTCACTCTCTCCCTATTTCCCCACTCTTAGGTTTAAAAG
  TCTGTCACCTTTCGCTTGGTTTAAACTCGGAAAGGTCTCAGTGCACAGCAAAGTTGCAGG
  GCTGCGTCTGCACTACGGAGCCTCTAGATTGCTGAAAACAGTCTTATGGAAGGATAACAC
  ATTGTCTGTCACTGGCTGGTTGTAATGCAAGGAAGGGACAAAGATGAAATGGAAACATGT
  TCCTTTTTTGGTCATGATATCACTCCTCAGCTTATCCCCAAATCACCTGTTTCTGGCCCA
  GCTTATTCCAGACCCTGAAGATGTAGAGAGGGGGAACGACCACGGGACGCCAATCCCCAC
  CTCTGATAACGATGACAATTCGCTGGGCTATACAGGCTCCCGTCTTCGTCAGGAAGATTT
  TCCACCTCGCATTGTTGAACACCCTTCAGACCTGATTGTCTCAAAAGGAGAACCTGCAAC
  TTTGAACTGCAAAGCTGAAGGCCGCCCCACACCCACTATTGAATGGTACAAAGGGGGAGA
  GAGAGTGGAGACAGACAAAGATGACCCTCGCTCACACCGAATGTTGCTGCCGAGTGGATC
  TTTATTTTTCTTACGTATAGTACATGGACGGAAAAGTAGACCTGATGAAGGAGTCTATGT
  CTGTGTAGCAAGGAATTACCTTGGAGAGGCTGTGAGCCACAATGCATCGCTGGAAGTAGC
  CATACTTCGGGATGACTTCAGACAAAACCCTTCGGATGTCATGGTTGCAGTAGGAGAGCC
  TGCAGTAATGGAATGCCAACCTCCACGAGGCCATCCTGAGCCCACCATTTCATGGAAGAA
  AGATGGCTCTCCACTGGATGATAAAGATGAAAGAATAACTATACGAGGAGGAAAGCTCAT
  GATCACTTACACCCGTAAAAGTGACGCTGGCAAATATGTTTGTGTTGGTACCAATATGGT
  TGGGGAACGTGAGAGTGAAGTAGCCGAGCTGACTGTCTTAGAGAGACCATCATTTGTGAA
  GAGACCCAGTAACTTGGCAGTAACTGTGGATGACAGTGCAGAATTTAAATGTGAGGCCCG
  AGGTGACCCTGTACCTACAGTACGATGGAGGAAAGATGATGGAGAGCTGCCCAAATCCAG
  ATATGAAATCCGAGATGATCATACCTTGAAAATTAGGAAGGTGACAGCTGGTGACATGGG
  TTCATACACTTGTGTTGCAGAAAATATGGTGGGCAAAGCTGAAGCATCTGCTACTCTGAC
  TGTTCAAGAACCTCCACATTTTGTTGTGAAACCCCGTGACCAGGTTGTTGCTTTGGGACG
  GACTGTAACTTTTCAGTGTGAAGCAACCGGAAATCCTCAACCAGCTATTTTCTGGAGGAG
  AGAAGGGAGTCAGAATCTACTTTTCTCATATCAACCACCACAGTCATCCAGCCGATTTTC
  AGTCTCCCAGACTGGCGACCTCACAATTACTAATGTCCAGCGATCTGATGTTGGTTATTA
  CATCTGCCAGACTTTAAATGTTGCTGGAAGCATCATCACAAAGGCATATTTGGAAGTTAC
  AGATGTGATTGCAGATCGGCCTCCCCCAGTTATTCGACAAGGTCCTGTGAATCAGACTGT
  AGCCGTGGATGGCACTTTCGTCCTCAGCTGTGTGGCCACAGGCAGTCCAGTGCCCACCAT
  TCTGTGGAGAAAGGATGGAGTCCTCGTTTCAACCCAAGACTCTCGAATCAAACAGTTGGA
  GAATGGAGTACTGCAGATCCGATATGCTAAGCTGGGTGATACTGGTCGGTACACCTGCAT
  TGCATCAACCCCCAGTGGTGAAGCAACATGGAGTGCTTACATTGAAGTTCAAGAATTTGG
  AGTTCCAGTTCAGCCTCCAAGACCTACTGACCCAAATTTAATCCCTAGTGCCCCATCAAA
  ACCTGAAGTGACAGATGTCAGCAGAAATACAGTCACATTATCGTGGCAACCAAATTTGAA
  TTCAGGAGCAACTCCAACATCTTATATTATAGAAGCCTTCAGCCATGCATCTGGTAGCAG
  CTGGCAGACCGTAGCAGAGAATGTGAAAACAGAAACATCTGCCATTAAAGGACTCAAACC
  TAATGCAATTTACCTTTTCCTTGTGAGGGCAGCTAATGCATATGGAATTAGTGATCCAAG
  CCAAATATCAGATCCAGTGAAAACACAAGATGTCCTACCAACAAGTCAGGGGGTGGACCA
  CAAGCAGGTCCAGAGAGAGCTGGGAAATGCTGTTCTGCACCTCCACAACCCCACCGTCCT
  TTCTTCCTCTTCCATCGAAGTGCACTGGACAGTAGATCAACAGTCTCAGTATATACAAGG
  ATATAAAATTCTCTATCGGCCATCTGGAGCCAACCACGGAGAATCAGACTGGTTAGTTTT
  TGAAGTGAGGACGCCAGCCAAAAACAGTGTGGTAATCCCTGATCTCAGAAAGGGAGTCAA
  CTATGAAATTAAGGCTCGCCCTTTTTTTAATGAATTTCAAGGAGCAGATAGTGAAATCAA
  GTTTGCCAAAACCCTGGAAGAAGCACCCAGTGCCCCACCCCAAGGTGTAACTGTATCCAA
  GAATGATGGAAACGGAACTGCAATTCTAGTTAGTTGGCAGCCACCTCCAGAAGACACTCA
  AAATGGAATGGTCCAAGAGTATAAGGTTTGGTGTCTGGGCAATGAAACTCGATACCACAT
  CAACAAAACAGTGGATGGTTCCACCTTTTCCGTGGTCATTCCCTTTCTTGTTCCTGGAAT
  CCGATACAGTGTGGAAGTGGCAGCCAGCACTGGGGCTGGGTCTGGGGTAAAGAGTGAGCC
  TCAGTTCATCCAGCTGGATGCCCATGGAAACCCTGTGTCACCTGAGGACCAAGTCAGCCT
  CGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCATAGCAGGTATTGGAGCAGC
  CTGTTGGATCATCCTCATGGTCTTCAGCATCTGGCTTTATCGACACCGCAAGAAGAGAAA
  CGGACTTACTAGTACCTACGCGGGTATCAGAAAAGTCCCGTCTTTTACCTTCACACCAAC
  AGTAACTTACCAGAGAGGAGGCGAAGCTGTCAGCAGTGGAGGGAGGCCTGGACTTCTCAA
  CATCAGTGAACCTGCCGCGCAGCCATGGCTGGCAGACACGTGGCCTAATACTGGCAACAA
  CCACAATGACTGCTCCATCAGCTGCTGCACGGCAGGCAATGGAAACAGCGACAGCAACCT
  CACTACCTACAGTCGCCCAGCTGATTGTATAGCAAATTATAACAACCAACTGGATAACAA
  ACAAACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGTGGACCTTAGTAACAA
  AATCAATGAGATGAAAACCTTCAATAGCCCAAATCTGAAGGATGGGCGTTTTGTCAATCC
  ATCAGGGCAGCCTACTCCTTACGCCACCACTCAGCTCATCCAGTCAAACCTCAGCAACAA
  CATGAACAATGGCAGCGGGGACTCTGGCGAGAAGCACTGGAAACCACTGGGACAGCAGAA
  ACAAGAAGTGGCACCAGTTCAGTACAACATCGTGGAGCAAAACAAGCTGAACAAAGATTA
  TCGAGCAAATGACACAGTTCCTCCAACTATCCCATACAACCAATCATACGACCAGAACAC
  AGGAGGATCCTACAACAGCTCAGACCGGGGCAGTAGTACATCTGGGAGTCAGGGGCACAA
  GAAAGGGGCAAGAACACCCAAGGTACCAAAACAGGGTGGCATGAACTGGGCAGACCTGCT
  TCCTCCTCCCCCAGCACATCCTCCTCCACACAGCAATAGCGAAGAGTACAACATTTCTGT
  AGATGAAAGCTATGACCAAGAAATGCCATGTCCCGTGCCACCAGCAAGGATGTATTTGCA
  ACAAGATGAATTAGAAGAGGAGGAAGATGAACGAGGCCCCACTCCCCCTGTTCGGGGAGC
  AGCTTCTTCTCCAGCTGCCGTGTCCTATAGCCATCAGTCCACTGCCACTCTGACTCCCTC
  CCCACAGGAAGAACTCCAGCCCATGTTACAGGATTGTCCAGAGGAGACTGGCCACATGCA
  GCACCAGCCCGACAGGAGACGGCAGCCTGTGAGTCCTCCTCCACCACCACGGCCGATCTC
  CCCTCCACATACCTATGGCTACATTTCAGGACCCCTGGTCTCAGATATGGATACGGATGC
  GCCAGAAGAGGAAGAAGACGAAGCCGACATGGAGGTAGCCAAGATGCAAACCAGAAGGCT
  TTTGTTACGTGGGCTTGAGCAGACACCTGCCTCCAGTGTTGGGGACCTGGAGAGCTCTGT
  CACGGGGTCCATGATCAACGGCTGGGGCTCAGCCTCAGAGGAGGACAACATTTCCAGCGG
  ACGCTCCAGTGTTAGTTCTTCGGACGGCTCCTTTTTCACTGATGCTGACTTTGCCCAGGC
  AGTCGCAGCAGCGGCAGAGTATGCTGGTCTGAAAGTAGCACGACGGCAAATGCAGGATGC
  TGCTGGCCGTCGACATTTTCATGCGTCTCAGTGCCCTAGGCCCACAAGTCCCGTGTCTAC
  AGACAGCAACATGAGTGCCGCCGTAATGCAGAAAACCAGACCAGCCAAGAAACTGAAACA
  CCAGCCAGGACATCTGCGCAGAGAAACCTACACAGATGATCTTCCACCACCTCCTGTGCC
  GCCACCTGCTATAAAGTCACCTACTGCCCAATCCAAGACACAGCTGGAAGTACGACCTGT
  AGTGGTGCCAAAACTCCCTTCTATGGATGCAAGAACAGACAGATCATCAGACAGAAAAGG
  AAGCAGTTACAAGGGGAGAGAAGTGTTGGATGGAAGACAGGTTGTTGACATGCGAACAAA
  TCCAGGTGATCCCAGAGAAGCACAGGAACAGCAAAATGACGGGAAAGGACGTGGAAACAA
  GGCAGCAAAACGAGACCTTCCACCAGCAAAGACTCATCTCATCCAAGAGGATATTCTACC
  TTATTGTAGACCTACTTTTCCAACATCAAATAATCCCAGAGATCCCAGTTCCTCAAGCTC
  AATGTCATCAAGAGGATCAGGAAGCAGACAAAGAGAACAAGCAAATGTAGGTCGAAGAAA
  TATTGCAGAAATGCAGGTACTTGGAGGATATGAAAGAGGAGAAGATAATAATGAAGAATT
  AGAGGAAACTGAAAGCTGAAGACAACCAAGAGGCTTATGAGATCTAATGTGAAAATCATC
  ACTCAAGATGCCTCCTGTCAGATGACACATGACGCCAGATAAAATGTTCAGTGCAATCAG
  AGTGTACAAATTGTCGTTTTTATTCCTCTTATTGGGATATCATTTTAAAAACTTTATTGG
  GTTTTTATTGTTGTTGTTTGATCCCTAACCCTACAAAGAGCCTTCCTATTCCCCTCGCTG
  TTGGAGCAAACCATTATACCTTACTTCCAGCAAGCAAAGTGCTTTGACTTCTTGCTTCAG
  TCATCAGCCAGCAAGAGGGAACAAAACTGTTCTTTTGCATTTTGCCGCTGAGATATGGCA
  TTGCACTGCTTATATGCCAAGCTAATTTATAGCAAGATATTGATCAAATATAGAAAGTTG
  ATATTCAACCTCACAAGGGCTCTCAAAGTATAATCTTTCTATAGCCAACTGCTAATGCAA
  ATTAAAACATATTTCATTTTAACATGATTTCAAAATCAGTTTTTCATACTACCCTTTGCT
  GGAAGAAACTAAAAATATAGCAAATGCAGAACCACAAACAATTCGAATGGGGTAGAAACA
  TTGTAAATATTTACTCTTTGCAAACCCTGGTGGTATTTTATTTTGGCTTCATTTCAATCA
  TTGAAGTATATTCTTATTGGAAATGTACTTTTGGATAAGTAGGGCTAAGCCAGTTGGATC
  TCTGGTTGTCTAGTCATTGTCATAAGTAAACCTAGTAAAACCTTGTTCTATTTTTCAATC
  ATCAAAAAGTAATTATAAATACGTATTACAAACAAGTGGATGTTTTTAATGACCAATTGA
  GTAAGAACATCCCTGTCTTAACTGGCCTAAATTTCTTCTGGTAGTGTCAGTTCAACTTTC
  AGAAGTGCCACTTAAGGAAGTTTGATTTTTGTTTTTGTAATGCACTGTTTTTAATCTCTC
  TCTCTTTTTTTTTTTTTTTTTGGTTTTAAAAGCACAATCACTAAACTTTATTTGTAAACC
  ATTGTAACTATTAACCTTTTTTGTCTTATTGAAAAAAAAAATGTTGAGAAGCGTTTTTAA
  CCTGTTTTGTTAATGCTCTATGTTTGTATTTGGAATATTTGAATAATGACAGATGGTGAC
  GTAACATGCATACTTTATTGTGGGCCATGAACCAAATGGTTCTTACTTTTCCTGGACTTA
  AAGAAAAAAAGAGGTTTAAGTTTGTTGTGGCCAATGTCGAAACCTACAAGATTTCCTTAA
  AATCTCTAATAGAGGCATTACTTGCTTTCAATTGACAAATGATGCCCTCTGACTAGTAGA
  TTTCTATGATCCTTTTTTGTCATTTTATGAATATCATTGATTTTATAATTGGTGCTATTT
  GAAGAAAAAAATGTACATTTATTCATAGATAGATAAGTATCAGGTCTGACCCCAGTGGAA
  AACAAAGCCAAACAAAACTGAACCACAAAAAAAAAGGCTGGTGTTCACCAAAACCAAACT
  TGTTCATTTAGATAATTTGAAAAAGTTCCATAGAAAAGGCGTGCAGTACTAAGGGAACAA
  TCCATGTGATTAATGTTTTCATTATGTTCATGTAAGAAGCCCCTTATTTTTAGCCATAAT
  TTTGCATACTGAAAATCCAATAATCAGAAAAGTAATTTTGTCACATTATTTATTAAAAAT
  GTTCTCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002941
• Insert Size: 6900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_002941.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002941.2, NP_002932.1
• RefSeq Size: 6629 bp
• RefSeq ORF: 4956 bp
• Locus ID: 6091
• UniProt ID: Q9Y6N7
• Cytogenetics: 3p12.3
• Domains: ig, IGv, IGc2, IG, FN3
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance
• Gene Summary: Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (1) encodes the longest isoform (a).