RAD51D (NM_133629) Human Untagged Clone

CAT#: SC109708

RAD51D (untagged)-Human RAD51-like 3 (S. cerevisiae) (RAD51L3), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAD51D
• Synonyms: BROVCA4; R51H3; RAD51L3; TRAD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_133629, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCGTGCTCAGGGTCGGACTGTGCCCTGGCCTTACCGAGGAGATGATCCAGCTTCTCAGGAGCCACA
  GGATCAAGACAGTGGTGGACCTGGTTTCTGCAGACCTGGAAGAGGTAGCTCAGAAATGTGGCTTGTCTTA
  CAAGGCAGAAGCTCTCCGGAGGATCCAGGTGGTGCATGCATTTGACATCTTCCAGATGCTGGATGTGCTG
  CAGGAGCTCCGAGGCACTGTGGCCCAGCAGGTGACTGGTTCTTCAGGAACTGTGAAGGTGGTGGTTGTGG
  ACTCGGTCACTGCGGTGGTTTCCCCACTTCTGGGAGGTCAGCAGAGGGAAGGCTTGGCCTTGATGATGCA
  GCTGGCCCGAGAGCTGAAGACCCTGGCCCGGGACCTTGGCATGGCAGTGGTGGTGACCAACCACATAACT
  CGAGACAGGGACAGCGGGAGGCTCAAACCTGCCCTCGGACGCTCCTGGAGCTTTGTGCCCAGCACTCGGA
  TTCTCCTGGACACCATCGAGGGAGCAGGAGCATCAGGCGGCCGGCGCATGGCGTGTCTGGCCAAATCTTC
  CCGACAGCCAACAGGTTTCCAGGAGATGGTAGACATTGGGACCTGGGGGACCTCAGAGCAGAGTGCCACA
  TTACAGGGTGATCAGACATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_133629 unedited
  AATTGTACCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT
  AAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCC
  GCGAATTCGGCACGAGGGCGGGCGGGCTCTGGGCGCGGGAATCCCGGCGGATCCCGGGCG
  GGCGGATGACCCCCAGCCCTACCCTTGGTGCCGCCTCCTCCTCTCTCCTTTCTCCTCCGG
  CAGCCAGCGCGCCTGTGTCCTCTCTAGGAAGGGGTAGGGGAGGGGCGTCTGGAGAGGACC
  CCCCGCGAATGCCCACGTGACGTGCAGTCCCCCTGGGGCTGTTCCGGCCTGCGGGGAACA
  TGGGCGTGCTCAGGGTCGGACTGTGCCCTGGCCTTACCGAGGAGATGATCCAGCTTCTCA
  GGAGCCACAGGATCAAGACAGTGGTGGACCTGGTTTCTGCAGACCTGGAAGAGGTAGCTC
  AGAAATGTGGCTTGTCTTACAAGGCCCTGGTTGCCCTGAGGCGGGTGCTGCTGGCTCAGT
  TCTCGGCTTTCCCCGTGAATGGCGCTGATCTCTACGAGGAACTGAAGACCTCTACTGCCA
  TCCTGTCCACTGGCATTGGCAGTCTTGATAAACTGCTTGATGCTGGTCTCTATACTGGAG
  AAGTGACTGAAATTGTAGGAGGCCCAGGTAACGGCAAAACTCAGGTATGTCTCTGTATGG
  CAGCAAATGTGGCCCATGGCCTGCAGCAAAACGTCCTATATGTAGATTCCAATGGAGGGC
  TGACAGCTTCCCGCCTCCTCCAGCTGCTTCNAGCTAAAACCCCAGATGAGGAGGAAACAG
  GCAGAGCTCTCCGGGAGATCCCAGTGGTGCATGCATTTGACATCTTCCAGATGCTGGATG
  TGCTGCNAGAGCTCCGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_133629
• Insert Size: 2650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133629.1, NP_598332.1
• RefSeq Size: 1477 bp
• RefSeq ORF: 651 bp
• Locus ID: 5892
• UniProt ID: O75771
• Cytogenetics: 17q12
• Domains: ENDO3c
• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination
• Gene Summary: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, which are known to be involved in the homologous recombination and repair of DNA. This protein forms a complex with several other members of the RAD51 family, including RAD51L1, RAD51L2, and XRCC2. The protein complex formed with this protein has been shown to catalyze homologous pairing between single- and double-stranded DNA, and is thought to play a role in the early stage of recombinational repair of DNA. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream ring finger and FYVE-like domain containing 1 (RFFL) gene. [provided by RefSeq, Jan 2011]
  Transcript Variant: This variant (4, also known as TRAD-d3) lacks three alternate exons which results in the loss of an in-frame segment in the central coding region, compared to variant 1. The encoded isoform (4) is shorter than isoform 1.