INPP5F (OCRL) (NM_000276) Human Untagged Clone

CAT#: SC109507

OCRL (untagged)-Human oculocerebrorenal syndrome of Lowe (OCRL), transcript variant a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: INPP5F
• Synonyms: Dent-2; DENT2; INPP5F; LOCR; NPHL2; OCRL-1; OCRL1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000276 edited
  ATGGAGCCGCCGCTCCCGGTCGGAGCCCAGCCGCTTGCCACTGTCGAGGGTATGGAGATG
  AAGGGTCCTCTCCGGGAGCCCTGCGCCCTGACCCTAGCCCAGAGGAACGGGCAATATGAG
  TTAATAATCCAGTTGCATGAGAAGGAACAGCATGTTCAAGATATCATTCCTATAAATAGC
  CACTTCAGATGTGTTCAAGAAGCAGAAGAAACTCTTTTGATTGACATAGCTTCTAACAGT
  GGCTGCAAAATTCGGGTTCAGGGGGACTGGATCAGAGAGCGCCGCTTTGAAATCCCTGAT
  GAGGAACACTGTTTGAAGTTCCTCTCAGCTGTCCTTGCTGCTCAGAAAGCTCAGTCACAG
  CTTCTTGTTCCAGAGCAAAAGGACTCATCTAGCTGGTACCAGAAATTAGACACTAAGGAC
  AAACCTTCTGTTTTTTCAGGGCTTCTTGGATTTGAAGACAATTTTTCTTCTATGAATTTG
  GACAAGAAAATAAATTCACAAAATCAGCCTACTGGGATTCATCGGGAACCCCCACCTCCA
  CCCTTTTCAGTGAATAAAATGCTTCCACGTGAAAAAGAAGCTTCTAACAAGGAGCAGCCC
  AAAGTGACCAACACCATGCGGAAGCTCTTTGTACCAAATACCCAATCTGGGCAGCGGGAG
  GGTCTCATCAAACATATCCTGGCAAAGCGAGAGAAAGAATATGTCAACATTCAGACTTTC
  AGATTTTTTGTTGGAACTTGGAATGTGAATGGCCAGTCTCCAGATAGCGGGTTAGAACCT
  TGGCTGAACTGTGATCCCAATCCTCCTGATATCTACTGCATTGGATTCCAAGAACTGGAC
  TTGAGCACAGAAGCCTTCTTCTACTTTGAATCTGTGAAGGAACAAGAATGGTCCATGGCT
  GTAGAGAGAGGTTTGCATTCCAAAGCCAAGTATAAGAAAGTTCAACTGGTGCGCCTTGTT
  GGGATGATGCTTCTTATATTTGCCAGAAAGGATCAGTGTCGATACATTCGTGATATTGCT
  ACAGAAACAGTTGGAACTGGAATCATGGGGAAAATGGGAAACAAAGGTGGGGTAGCTGTG
  AGATTTGTATTTCACAACACCACCTTTTGCATTGTCAATTCCCATCTGGCTGCACACGTG
  GAGGACTTTGAGAGAAGGAATCAAGATTATAAGGACATTTGTGCGAGAATGAGTTTTGTG
  GTCCCAAATCAGACCCTCCCGCAGTTGAACATCATGAAACATGAGGTTGTCATTTGGTTG
  GGAGATTTGAATTATAGACTTTGCATGCCTGATGCCAATGAGGTGAAAAGTCTTATTAAT
  AAGAAAGACCTTCAGAGACTCTTGAAATTCGACCAGCTAAATATTCAGCGCACACAGAAA
  AAAGCTTTTGTTGACTTCAATGAAGGGGAAATCAAGTTCATCCCCACTTATAAGTATGAC
  TCTAAAACAGACCGGTGGGATTCCAGTGGGAAATGCCGGGTTCCAGCCTGGTGTGACCGA
  ATTCTTTGGAGAGGAACAAATGTTAATCAGCTTAATTATCGGAGTCACATGGAACTGAAA
  ACCAGCGACCACAAGCCTGTTAGCGCCCTCTTCCATATTGGGGTGAAGGTTGTGGATGAA
  CGAAGGTACCGGAAAGTCTTTGAAGATAGTGTACGCATCATGGACAGAATGGAAAATGAC
  TTCCTTCCTTCCTTAGAACTCAGCAGGAGGGAGTTTGTGTTTGAAAATGTGAAGTTTCGG
  CAACTACAAAAGGAGAAGTTCCAGATCAGCAACAATGGACAGGTTCCCTGCCATTTTTCT
  TTCATCCCTAAACTTAATGACAGCCAGTACTGCAAGCCATGGCTTCGGGCTGAACCTTTT
  GAGGGCTACTTGGAGCCAAATGAGACAGTGGACATTTCTCTTGATGTGTATGTCAGCAAA
  GACTCTGTAACCATCCTGAACTCGGGAGAAGATAAGATTGAAGATATTCTCGTCCTTCAC
  CTGGATCGAGGCAAAGATTACTTCTTGACTATCAGTGGAAATTACCTCCCAAGTTGTTTT
  GGCACATCCTTAGAGGCTCTGTGCCGTATGAAAAGACCAATCCGAGAAGTTCCTGTTACC
  AAACTCATAGACTTGGAAGAAGACAGCTTCCTAGAAAAGGAGAAATCCCTTCTGCAAATG
  GTTCCTTTGGATGAAGGTGCCAGTGAGAGACCCCTTCAGGTTCCCAAGGAGATCTGGCTT
  CTAGTAGATCACCTATTCAAATACGCCTGTCACCAGGAGGACCTGTTCCAGACCCCTGGA
  ATGCAGGAAGAGCTCCAGCAGATCATTGATTGTCTGGATACCAGCATTCCTGAGACAATC
  CCTGGCAGCAACCACTCTGTGGCTGAAGCACTGCTCATTTTCTTGGAAGCCCTGCCAGAG
  CCAGTCATCTGTTACGAGCTGTATCAGCGATGTCTTGACTCTGCTTATGATCCCCGGATC
  TGCCGACAGGTGATCTCCCAGCTTCCGAGATGCCATAGAAATGTTTTCCGTTACTTGATG
  GCATTCCTTCGAGAACTCTTAAAATTCTCTGAATACAATAGCGTCAATGCCAACATGATC
  GCTACTCTCTTCACTAGTCTTCTCCTGAGGCCTCCACCCAACCTTATGGCAAGACAGACT
  CCAAGTGACCGCCAGCGTGCTATTCAGTTCCTTCTGGGCTTTCTGCTTGGGAGCGAAGAA
  GACTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000276 unedited
  CGAGGCCGCTCCCGGCTCCCGGCGCCCGGCGCCCGGCGCGGAGCTGTTCCTCAAACGACA
  CGCAGCCGAGGTGGGTGGGTGTGGGGACGCGGGAGCCAGTGTCGTCGGATCGGCCCGCAG
  TCCGCTGTCCTGCTGAGCCCGGAGGCCGCCTGGATGGAGCCGCCGCTCCCGGTCGGAGCC
  CAGCCGCTTGCCACTGTCGAGGGTATGGAGATGAAGGGTCCTCTCCGGGAGCCCTGCGCC
  CTGACCCTAGCCCAGAGGAACGGGCAATATGAGTTAATAATCCAGTTGCATGAGAAGGAA
  CAGCATGTTCAAGATATCATTCCTATAAATAGCCACTTCAGATGTGTTCAAGAAGCAGAA
  GAAACTCTTTTGATTGACATAGCTTCTAACAGTGGCTGCAAAATTCGGGTTCAGGGGGAC
  TGGATCAGAGAGCGCCGCTTTGAAATCCCTGATGAGGAACACTGTTTGAAGTTCCCTCTC
  AGCTGTCCTTGCTGCTCAGAAAGCTCAGTCACAGCTTCTTGTTCCAGAGCANAAGGACTC
  ATCTAGCTGGTACCAGAAATTAGACACTAAGGACANACCTTCTGTTTTTTCAGGGCTTCT
  TGGATTTGAAGACAATTTTTCTTCTATGAATTTGGACAAGAAAATAAATTCACAAAATCA
  GCCTACTGGGATTTCATCGGGAACCCCACCTCCCACCCTTTTCAGTGAATTAAAATGCCT
  TCACCGTGAAAAAGAAACTTCTAACAAAGAGCAGCCCCAAGTGACCACCACCTGCCGAAG
  CTCCNTGTACCAAATCCCAATCTGGGCAGCGGAAGTTCCCATTAAACTATTCTGGCCAAG
  CGAGAAAAGATTTGTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000276 unedited
  ACTATGGACGCGGCCGCAATCTAGAATTTATTCANAAAAAATTTGCAATAAATTATAATA
  AAAAAGAGACACGAAATATAGAATCCCACTAGCAGCTTTGCTTAGTGATCAAGACGTTTT
  TGGGAAATATAACAAACAATTACAGAATAAGAAAACAGTGAAAGGGAGACTAATGCAATG
  TCACCACATGGATTTGAACCATTTCTTCCCTAAGACAGCAGGTGCAGAGTCTTTCCCTAC
  CAGCTGAATCCGAAATCCTTACTTCAGAGCAGCTACAAATTAGTACCTTAGAGGACTTGG
  CAATTACAGGTTGGGGTGCTGGAATAGCCATGGTAGGGAAGCAGATTTCTTCTGTCTGGG
  TCTACATCTTGTCTCCTCTACCTAGAGATTGTCTAAAACTCTCAACTCTGTATATGTTCC
  AGGATGAGCTGAAAAAAGGTCGAGGCAGTACACCAACTTAGTGTTTTGATCTGATGTAGA
  AAGAGATAGGATACTGCATACGTGCAGCACAGAGCCTCCACTAACCACACCCTTGATCAC
  CCAAAGATCCCAGGTCAGTGATATGCTAGAAAACAAGCCCAGGAAAGGTATTTCCCTGTG
  GTATGACCCTACCCCAGTGTCTATCATCTGCCTCTTTTTGTGATCACTTTCTGGCCTTGC
  GGAGATGACAAACCTGTTCTGCCCTCAGTCTTCCATTAACTAAGTTCAACACCTAGCCCC
  CTGACCTATCATACCTNCAGATCTGTGGCACCAGTAACTTGACTCCTATTGCTTTAACTC
  TCAATCACGAANAGTNTTTACCATATTGCANAATACGTACCTTCCCCCCCACCCCACCAA
  GAACAGGAGAAATCACTGTGGCGCCTTGGAATGCTAAAATGCACAGAGTCATGCCCAGAT
  GTACATAAGCCTGTACGTAGCTCCTGCAATCATGAAACAGGCAGGTTGGTGTACAAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000276
• Insert Size: 5130 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000276.3, NP_000267.2
• RefSeq Size: 5165 bp
• RefSeq ORF: 2706 bp
• Locus ID: 4952
• UniProt ID: Q01968
• Cytogenetics: Xq26.1
• Domains: RhoGAP, IPPc, Exo_endo_phos
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: This gene encodes an inositol polyphosphate 5-phosphatase. This protein is involved in regulating membrane trafficking and is located in numerous subcellular locations including the trans-Golgi network, clathrin-coated vesicles and, endosomes and the plasma membrane. This protein may also play a role in primary cilium formation. Mutations in this gene cause oculocerebrorenal syndrome of Lowe and also Dent disease. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (a) encodes isoform (a).