WNT5B (NM_030775) Human Untagged Clone

CAT#: SC109435

WNT5B (untagged)-Human wingless-type MMTV integration site family, member 5B (WNT5B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WNT5B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_030775 edited
  GAATTCGGCACGAGGATTTCTTTGGATGTTCTGCAGCAAGGACAGGTATGCTTTACAACA
  GCCGAAGTGGCCTCCCGGCTGCCGAACGGAGGAACGCCGCAAGCTCCGCTCTTGAAATTA
  CTTGTTTTCATTTCTCTTTGTGGTTTCTCAGCTCATTATTTCTTTGGAAATTAGGTCCTG
  TGCAGGGTTCCCACAGTTTGGGGTAAGAGACAGAAGTCCTAGGGTGGGAAGATGAGCAGT
  GGGAGGCGGAGGCTGGAAAGAGGCCGAGCTTCTTTGTGGGGAACACGCAGCACGTAAGCA
  TCAGTGCAACTTTCTCCGCCTCACCCCGGCTCCTGGTCTGCCCTTATCCGCTGAGTTTCC
  ACACTGACTCTCCATTTCTGTTTTCTCCAGGGAACCCTACTCTGGAAACTGTCAGTCCCA
  GGGCACTGGGGAGGGCTGAGGCCGACCATGCCCAGCCTGCTGCTGCTGTTCACGGCTGCT
  CTGCTGTCCAGCTGGGCTCAGCTTCTGACAGACGCCAACTCCTGGTGGTCATTAGCTTTG
  AACCCGGTGCAGAGACCCGAGATGTTTATCATCGGTGCCCAGCCCGTGTGCAGTCAGCTT
  CCCGGGCTCTCCCCTGGCCAGAGGAAGCTGTGCCAATTGTACCAGGAGCACATGGCCTAC
  ATAGGGGAGGGAGCCAAGACTGGCATCAAGGAATGCCAGCACCAGTTCCGGCAGCGGCGG
  TGGAATTGCAGCACAGCGGACAACGCATCTGTCTTTGGGAGAGTCATGCAGATAGGCAGC
  CGAGAGACCGCCTTCACCCACGCGGTGAGCGCCGCGGGCGTGGTCAACGCCATCAGCCGG
  GCCTGCCGCGAGGGCGAGCTCTCCACCTGCGGCTGCAGCCGGACGGCGCGGCCCAAGGAC
  CTGCCCCGGGACTGGCTGTGGGGCGGCTGTGGGGACAACGTGGAGTACGGCTACCGCTTC
  GCCAAGGAGTTTGTGGATGCCCGGGAGCGAGAGAAGAACTTTGCCAAAGGATCAGAGGAG
  CAGGGCCGGGTGCTCATGAACCTGCAAAACAACGAGGCCGGTCGCAGGGCTGTGTATAAG
  ATGGCAGACGTAGCCTGCAAATGCCACGGCGTCTCGGGGTCCTGCAGCCTCAAGACCTGC
  TGGCTGCAGCTGGCCGAGTTCCGCAAGGTCGGGGACCGGCTGAAGGAGAAGTACGACAGC
  GCGGCCGCCATGCGCGTCACCCGCAAGGGCCGGCTGGAGCTGGTCAACAGCCGCTTCACC
  CAGCCCACCCCGGAGGACCTGGTCTATGTGGACCCCAGCCCCGACTACTGCCTGCGCAAC
  GAGAGCACGGGCTCCCTGGGCACGCAGGGCCGCCTCTGCAACAAGACCTCGGAGGGCATG
  GATGGCTGTGAGCTCATGTGCTGCGGGCGTGGCTACAACCAGTTCAAGAGCGTGCAGGTG
  GAGCGCTGCCACTGCAAGTTCCACTGGTGCTGCTTCGTCAGGTGTAAGAAGTGCACGGAG
  ATCGTGGACCAGTACATCTGTAAATAGCCCGGAGGGCCTGCTCCCGGCCCCCCTGCACTC
  TGCCTCACAAAGGTCTATATTATATAAATCTATATAAATCTATTTTATATTTGTATAAGT
  AAATGGGTGGGTGCTATACAATGGAAAGATGAAAATGGAAAGGAAGAGCTTATTTAAGAG
  ACGCTGGAGATCTCTGAGGAGTGGACTTTGCTGGTTCTCTCCTCTTGGTGGGTGGGAGAC
  AGGGCTTTTTCTCTCCCTCTGGTGAGGACTCTCAGGATGTAGGGACTTGGAAATATTTAC
  TGTCTGTCCACCACGGCCTGGAGGAGGGAGGTTGTGGTTGGATGGAGGAGATGATCTTGT
  CTGGAAGTCTAGAGTCTTTGTTGGTTAGAGGACTGCCTGTGATCCTGGCCACTAGGCCAA
  GAGGCCCTATGAAGGTGGCGGGAACTCAGCTTCAACCTCGATGTCTTCAGGGTCTTGTCC
  AGAATGTAGATGGGTTCCGTAAGAGGCCTGGTGCTCTCTTACTCTTTCATCCACGTGCAC
  TTGTGCGGCATCTGCAGTTTACAGGAACGGCTCCTTCCCTAAAATGAGAAGTCCAAGGTC
  ATCTCTGGCCCAGTGACCACAGAGAGATCTGCACCTCCCGGACTTCAGGCCTGCCTTTCC
  AGCGAGAATTCTTCATCCTCCACGGTTCACTAGCTCCTACCTGAAGAGGAAAGGGGGCCA
  TTTGACCTGACATGTCAGGAAAGCCCTAAACTGAATGTTTGCGCCTGGGCTGCAGAAGCC
  AGGGTGCATGACCAGGCTGCGTGGACGTTATACTGTCTTCCCCCACCCCCGGGGAGGGGA
  AGCTTGAGCTGCTGCTGTCACTCCTCCACCGAGGGAGGCCTCACAAACCACAGGACGCTG
  CAACGGGTCAGGCTGGCGGGCCCGGCGTGCTCATCATCTCTGCCCCAGGTGTACGGTTTC
  TCTCTGACATTAAATGCCCTTCATGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030775 unedited
  CATATTATGNATACGACTCACTATAGGGCGGCCCGCGAATCGGCACGAGGATTTCTTTGG
  ATGTTCTGCAGCAAGGACAGGTATGCTTTACAACAGCCGAAGTGGCCTCCCGGCTGCCGA
  ACGGAGGAACGCCGCAAGCTCCGCTCTTGAAATTACTTGTTTTCATTTCTCTTTGTGGTT
  TCTCAGCTCATTATTTCTTTGGAAATTAGGTCCTGTGCAGGGTTCCCACAGTTTGGGGTA
  AGAGACAGAAGTCCTAGGGTGGGAAGATGAGCAGTGGGAGGCGGAGGCTGGAAAGAGGCC
  GAGCTTCTTTGTGGGGAACACGCAGCACGTAAGCATCAGTGCAACTTTCTCCGCCTCACC
  CCGGCTCCTGGTCTGCCCTTATCCGCTGAGTTTCCACACTGACTCTCCATTTCTGTTTTC
  TCCAGGGAACCCTACTCTGGAAACTGTCAGTCCCAGGGCACTGGGGAGGGCTGAGGCCGA
  CCATGCCCAGCCTGCTGCTGCTGTTCACGGCTGCTCTGCTGTCCAGCTGGGCTCAGCTTC
  TGACAGACGCCAACTCCTGGTGGTCATTAGCTTTGAACCCGGTGCAGAGACCCGAGATGT
  TTATCATCGGTGCCCAGCCCGTGTGCAGTCAGCTTCCCGGGCTCTCCCCTGGCCAGAGGA
  AGCTGTGCCAATTGTACCAGGAGCACATGGCCTACATAGGGGAGGGAGCCAAGACTGGCA
  TCAAGGAATGCCAGCACCAGTTCCGGCAGCGGCGGTGGGATTGCAGCACAGCGGACAACG
  CATCTGTCTTTGGGAGAGTCATGCAGATAGGCAGCCGAGAGACCGNCTTCACCCACGCCG
  TGAGCGCCGCGGGCGTGGTCAACGCCATCAGCCGGGCCTGCCGCGAGGGCGAGCTCTNCA
  CCTGCGNTGCAGCCGGACGGNGCGGCCCAGGACCTGCCCGGACTGGCTGTGNGCCGCTGT
  GGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030775 unedited
  NNTTTTTTNNGGGGCATTTATGTNCAGTAGGNAACCGTACACCTGGGGCAGNANAGATGA
  GCACGCCGGGCCCGCCAGCCTGACCCGTTGCAGCGTCCTGTGGTTTGTGAGGCCTCCCTC
  GGTGGAGGAGTGACAGCAGCAGCTCAAGCTTCCCCTCCCCGGGGGTGGGGGAAGACAGTA
  TAACGTCCACGCAGCCTGGTCATGCACCCTGGCTTCTGCAGCCCAGGCGCAAACATTCAG
  TTTAGGGCTTTCCTGACATGTCAGGTCAAATGGCCCCCTTTCCTCTTCAGGTAGGAGCTA
  GTGAACCGTGGAGGATGAAGAATTCTCGCTGGAAAGGCAGGCCTGAAGTCCGGGAGGTGC
  AGATCTCTCTGTGGTCACTGGGCCAGAGATGACCTTGGACTTCTCATTTTAGGGAAGGAG
  CCGTTCCTGTAAACTGCAGATGCCGCACAAGTGCACGTGGATGAAAGAGTAAGAGAGCAC
  CAGGCCTCTTACGGAACCCATCTACATTCTGGACAAGACCCTGAAGACATCGAGGTTGAA
  GCTGAGTTCCCGCCACCTTCATAGGGCCTCTTGGCCTAGTGGCCAGGATCACAGGCAGTC
  CTCTAACCAACAAAGACTCTAGACTTCCAGACAAGATCATCTCCTCCATCCAACCACAAC
  CTCCCTCCTNCAGGCCGTGGTGGACAGACAGTAAATATTTCCAAGTCCCTACATCCTGAG
  AGTCCTCACCANGAGGAGAGAAAAAGCCCTGTCTCCCACCCACCAAGAGGAGAGAACCAG
  CAAAGTCCACTCCTCAGAGATCTCCANCGTCTCTTAAATAGCTCTTCCTTTCCATTNTCA
  TCTTTCCATTGTATAGCACCCACCCCTTTACTTATACAAATATAAATAGATTTATATAGA
  TTTATATATATAGACCCTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_030775
• Insert Size: 2610 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030775.2, NP_110402.2
• RefSeq Size: 2184 bp
• RefSeq ORF: 1080 bp
• Locus ID: 81029
• UniProt ID: Q9H1J7
• Cytogenetics: 12p13.33
• Domains: wnt
• Protein Families: Secreted Protein
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Melanogenesis, Pathways in cancer, Wnt signaling pathway
• Gene Summary: The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs from variant 1 in the 5' UTR, which results from an alternate exon. Both variants encode the same protein.