Microsomal Glutathione S transferase 1 (MGST1) (NM_020300) Human Untagged Clone

CAT#: SC109434

MGST1 (untagged)-Human microsomal glutathione S-transferase 1 (MGST1), transcript variant 1b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Microsomal Glutathione S transferase 1
• Synonyms: GST12; MGST; MGST-I
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020300, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTTGACCTCACCCAGGTAATGGATGATGAAGTATTCATGGCTTTTGCATCCTATGCAACAATTATTC
  TTTCAAAAATGATGCTTATGAGTACTGCAACTGCATTCTATAGATTGACAAGAAAGGTTTTTGCCAATCC
  AGAAGACTGTGTAGCATTTGGCAAAGGAGAAAATGCCAAGAAGTATCTTCGAACAGATGACAGAGTAGAA
  CGTGTACGCAGAGCCCACCTGAATGACCTTGAAAATATTATTCCATTTCTTGGAATTGGCCTCCTGTATT
  CCTTGAGTGGTCCCGACCCCTCTACAGCCATCCTGCACTTCAGACTATTTGTCGGAGCACGGATCTACCA
  CACCATTGCATATTTGACACCCCTTCCCCAGCCAAATAGAGCTTTGAGTTTTTTTGTTGGATATGGAGTT
  ACTCTTTCCATGGCTTACAGGTTGCTGAAAAGTAAATTGTACCTGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020300 unedited
  ACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCGGGACAGGCTTGCTGCTTCCTCCTC
  CTCGGCCTCACCATTCCAGACCAAAATGAAAAAAGGTTGACCTCACCCAGGTAATGGATG
  ATGAAGTATTCATGGCTTTTGCATCCTATGCAACAATTATTCTTTCAAAAATGATGCTTA
  TGAGTACTGCAACTGCATTCTATAGATTGACAAGAAAGGTTTTTGCCAATCCAGAAGACT
  GTGTAGCATTTGGCAAAGGAGAAAATGCCAAGAAGTATCTTCGAACAGATGACAGAGTAG
  AACGTGTACGCAGAGCCCACCTGAATGACCTTGAAAATATTATTCCATTTCTTGGAATTG
  GCCTCCTGTATTCCTTGAGTGGTCCCGACCCCTCTACAGCCATCCTGCACTTCAGACTAT
  TTGTCGGAGCACGGATCTACCACACCATTGCATATTTGACACCCCTTCCCCAGCCAAAAT
  AGAGCTTTGAGTCTTTTTGGTGGATATGGAGTTACTCTTTCCATGGCTTACAGGTTGCTG
  AAAAGAAATTGTACCCTGTAAGAAATCATACCACTCAGCATCCAGCTGGCTTTTTAAGAA
  CTCTGTACCTCCAATTTATAATGACTACTTTCTTAGATTTTACGTAGGAGGGCAGCAGAG
  GAATTATGAACTGGGGTACACCCATTTTGACTATTACATTGCCAATATCCTGTATTCTGG
  TTTACATTCGGATTAAAAATTAACATAGAGATTCCTAAGCCTTTTGCCTGAGTTTTAACG
  ACCTTCCTAAAAATGCGATCATGTTTAGATTNGTAACATTCCACCACACCTTACTTTTAG
  ATCTATAAAGAATTGCCCGTTGAGAAACCTTATTTCCAATCTGCTGGAACAGACTGAAAT
  AGGAATTTAAGAAACAAAAAAACAAANACCCCACTCTAAATTGGGCCGGGTATAGTTGTT
  CCTGAAATCCCGTTGAATCCTGGGACCCTCCAGGCTTTCTGGCCTGAATGACCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020300 unedited
  GCTATACTCGAGTTTTTTTTTTTTTTTTTTTTTCTTTAAATTCTTTATTTCATGTCTGTT
  TCAGCAGTATTGAAATATAGGTTTCTCATACGTGCAATTCTTTTATAGATTCAAAAGTGA
  GGTGTTGTGTGAATGTTACAAATCATAACATGATCCAAATTTATAAGAAAGTACTTTAAA
  AATCAGACAAAAGACTTAAGAATTACTATGTTAAATTTCTAATCCAAATGTAAAACAAGA
  ATACAGGATATTGGCAATGCTAATATTCAAAATGGGTTTACCCCAGTTCATAATACCTCT
  GCTACCCTCCTACCTAAAATCTAAGAAAGTATTCATTATAAATTGGAAGCACAGAATTCT
  TAAAAAGCCAACTGGATGCTGAGTTGTATGATTTTCTTTACAGGTACCAATCTACTTTTA
  AGCAACCTGTAAGCCATGGAAAGAGCACCTCCATATACAAAATAAAAACTCAAAGCTCTA
  TTTGGCTGGGGAAGGTGCGCCAAATATGCAATGGCGGGGCAAATCCCGTGCTCCGACAAA
  TAGTCCTGAATTGCAGGCATGGTTGTACAGGGGCCGTCACCACTCATGGAATACGGGAGG
  CCTTTCCCACCAACTGGAATATCATTCCTAGGCACTTTACGTGGGCTTTGGGCACCCGTT
  CCACTTTGGTATTCTGTCCGAAAAATCCTTCTTGAACTTATCCTCCTTTGCCCAAAGGGC
  TCAAAACGTCCTCTGGGATTGGCAACAACCTTTTCTTGTCAATTCTTATCAATGCCCGTG
  GCGTACTCTATACCCCTCCTTTTTGTAACAAATATTGCTGTCTAAGAATCCACAGCCCTG
  TAATCCTTTCATATCACATTACCGCGGCGGAGGGCAACCATTTCTTAACTCTTCGCTCGA
  CAGTGGGAGGCCCCGGGGGAGAACACCCACCCTTGTCCCGCGCTCTCTCCCGAATTTGCG
  CGCGCCCTTATGTGGAGATCCATACACCCCTCGCGGTCCATAAACGTCCTGCTTTAATAC
  CTCTCCCCTCAACTCTCTCGTCTATTTTGTCACGGCGCGGTTTTCAAACTTTGTCACACC
  CTCTANTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020300
• Insert Size: 910 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020300.3, NP_064696.1
• RefSeq Size: 953 bp
• RefSeq ORF: 468 bp
• Locus ID: 4257
• UniProt ID: P10620
• Cytogenetics: 12p12.3
• Domains: MAPEG
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450
• Gene Summary: The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family consists of six human proteins, two of which are involved in the production of leukotrienes and prostaglandin E, important mediators of inflammation. Other family members, demonstrating glutathione S-transferase and peroxidase activities, are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. This gene encodes a protein that catalyzes the conjugation of glutathione to electrophiles and the reduction of lipid hydroperoxides. This protein is localized to the endoplasmic reticulum and outer mitochondrial membrane where it is thought to protect these membranes from oxidative stress. Several transcript variants, some non-protein coding and some protein coding, have been found for this gene. [provided by RefSeq, May 2012]
  Transcript Variant: This variant (2, also called 1b) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3, 4, and 5 all encode the same isoform (a).