HYAL1 (NM_007312) Human Untagged Clone

CAT#: SC109328

HYAL1 (untagged)-Human hyaluronoglucosaminidase 1 (HYAL1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HYAL1
• Synonyms: HYAL-1; LUCA1; MGC45987; NAT6
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109328 sequence for NM_007312 edited (data generated by NextGen Sequencing)
  ATGGCAGCCCACCTGCTTCCCATCTGCGCCCTCTTCCTGACCTTACTCGATATGGCCCAA
  GGCTTTAGGGGCCCCTTGCTACCCAACCGGCCCTTCACCACCGTCTGGAATGCAAACACC
  CAGTGGTGCCTGGAGAGGCACGGTGTGGACGTGGATGTCAGTGTCTTCGATGTGGTAGCC
  AACCCAGGGCAGACCTTCCGCGGCCCTGACATGACAATTTTCTATAGCTCCCAGCTGGGC
  ACCTACCCCTACTACACGCCCACTGGGGAGCCTGTGTTTGGTGGTCTGCCCCAGAATGCC
  AGCCTGATTGCCCACCTGGCCCGCACATTCCAGGACATCCTGGCTGCCATACCTGCTCCT
  GACTTCTCAGGGCTGGCAGTCATCGACTGGGAGGCATGGCGCCCACGCTGGGCCTTCAAC
  TGGGACACCAAGGACATTTACCGGCAGCGCTCACGGGCACTGGTACAGGCACAGCACCCT
  GATTGGCCAGCTCCTCAGGTGGAGGCAGTAGCCCAGGACCAGTTCCAGGGAGCTGCACGG
  GCCTGGATGGCAGGCACCCTCCAGCTGGGGCGGGCACTGCGTCCTCGCGGCCTCTGGGGC
  TTCTATGGCTTCCCTGACTGCTACAACTATGACTTTCTAAGCCCCAACTACACCGGCCAG
  TGCCCATCAGGCATCCGTGCCCAAAATGACCAGCTAGGGTGGCTGTGGGGCCAGAGCCGT
  GCCCTCTATCCCAGCATCTACATGCCCGCAGTGCTGGAGGGCACAGGGAAGTCACAGATG
  TATGTGCAACACCGTGTGGCCGAGGCATTCCGTGTGGCTGTGGCTGCTGGTGACCCCAAT
  CTGCCGGTGCTGCCCTATGTCCAGATCTTCTATGACACGACAAACCACTTTCTGCCCCTG
  GATGAGCTGGAGCACAGCCTGGGGGAGAGTGCGGCCCAGGGGGCAGCTGGAGTGGTGCTC
  TGGGTGAGCTGGGAAAATACAAGAACCAAGGAATCATGTCAGGCCATCAAGGAGTATATG
  GACACTACACTGGGGCCCTTCATCCTGAACGTGACCAGTGGGGCCCTTCTCTGCAGTCAA
  GCCCTGTGCTCCGGCCATGGCCGCTGTGTCCGCCGCACCAGCCACCCCAAAGCCCTCCTC
  CTCCTTAACCCTGCCAGTTTCTCCATCCAGCTCACGCCTGGTGGTGGGCCCCTGAGCCTG
  CGGGGTGCCCTCTCACTTGAAGATCAGGCACAGATGGCTGTGGAGTTCAAATGTCGATGC
  TACCCTGGCTGGCAGGCACCGTGGTGTGAGCGGAAGAGCATGTGGTGA
  
  Clone variation with respect to NM_007312.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007312 unedited
  GGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACCAGNAATGGCAGA
  GGCCTGGTCTGAGAGGCAACTCGGATGTGCCCTCCAGTGGCCATGCTCCCCTCCATGCGT
  CTCCCCTGCCCTCCTGGAGCCCTGCAGGTCAATGTTTAACAGAAACCAGAGCAGCGGTGG
  ATTAATGCGCAAGGGCTCAGCCCCCCAGCCCTGAGCAGTGGGGGAATCGGAGACTTTGCA
  ACCTGTTCTCAGCTCTGCCTCCCCTGGCCAGGTTGTCCTCGACCAGTCCCGTGCCATGGC
  AGCCCACCTGCTTCCCATCTGCGCCCTCTTCCTGACCTTACTCGATATGGCCCAAGGCTT
  TAGGGGCCCCTTGCTACCCAACCGGCCCTTCACCACCGTCTGGAATGCAAACACCCAGTG
  GTGCCTGGAGAGGCACGGTGTGGACGTGGATGTCAGTGTCTTCGATGTGGTAGCCAACCC
  AGGGCAGACCTTCCGCGGCCCTGACATGACAATTTTCTATAGCTCCCAGCTGGGCACCTA
  CCCCTACTACACGCCCACTGGGGAGCCTGTGTTTGGTGGTCTGCCCCAGAATGCCAGCCT
  GATTGCCCACCTGGCCCGCACATTCCAGGACATCCTGGCTGCCATACCTGCTCCTGACTT
  CTCAGGGCTGGCAGTCATCGACTGGGAGGCATGGCGCCCACGCTGGGCCTTCAACTGGGA
  CACCAAGGACATTTACCGGCAGCGCTCACGGGCACTGGTACAGGCACAGCACCCTGATTG
  GCCAGCTCCCTCAGTGGAGGCAGTAGCCCAGAACCAGTTCCAGGGAGCTGCACGGGCCTG
  GATGGCANGCACCCTCCAGCTGGGGCGGGCACTGCGTCCTCGCGGCCTCTGGGGCTTCTA
  TGGCTTNCCTGACTGCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007312 unedited
  NTACTNTGNNACCGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTT
  TTAAATACATGACTGCTTTATTTGCTGGACTGGTGCCTCTCAGCAGATTCAGGGGTCGTG
  CAGGGCTGGTTACCACAAACTCAGTAGGAGTGCAAGGGCTGTACCCCCGGAGCTAGACAG
  CCTGGGTTTGAATCTCAACTTCTCCCTTTTCTTGCTGTGCAACCTTGGGCAACGTGCTTA
  CTCTCAATGTGAGTGACTCAGTTTCCTCATAGCCTCATTGTGAGGAATGAATAAGTCCAC
  ATAAAACGCTTAGCACGGGGATTGGCAAAGAGTAGGCACTCAGTGAATGTCAGCCTTTGT
  GATTGCTGCAGTTCAAAGACTGGCTCAGTGTCTCTGGAGGAATTGTCTATGACCTTGCCA
  AGTAGATGCATATGGACATGGAATGAATGGTGTCTGCTGTGGTTCTAACTCCTTATGCCA
  CTATTCCAGTCTGTAAGTATGCATGTGTGTGCAGGGAATATGCCTGTGACAGTGGCTGAG
  TGTACTCTTTACTGTGACCATGACTTGTATGACTGTGCATGTATTTGAGGAAGCCCTGGC
  CAGACCCAGAGTGCATTAGGTTCTCAATATGTGCAACTCAGTGTGTGGCCAATCACCACA
  TGCTCTTCCGCTCACACCACGGTGCCTGCCAGCCAGGGTAGCATCGACATTTGAACTCCC
  AGCCATCTGTGCCTGATCTTCAAGTGAGAGGGCACCCCGCAGGCTCAGGGGGCCCACCAC
  CAGCGTGAGCTGGATGGANAAACTGNCAGGNTAANGGAGAGGAGGNCTTTGNGGTGGCTG
  GTGNCGCGGCACAGCGNCCATGGCCGNAGCACAGGCTTGACTGCANAAAGGNCCCACTGN
  NTCACGTCAGGATGAGGGCCCCAGNGTANTGTCATAACTNNCTGATGCCTGAATGATNCC
  TGGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007312
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007312.3, NP_009296.1
• RefSeq Size: 2518 bp
• RefSeq ORF: 1308 bp
• Locus ID: 3373
• Cytogenetics: 3p21.31
• Domains: Glyco_hydro_56
• Protein Families: Secreted Protein
• Protein Pathways: Glycosaminoglycan degradation, Lysosome, Metabolic pathways
• Gene Summary: This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).