CSHL1 (NM_001318) Human Untagged Clone

CAT#: SC109123

CSHL1 (untagged)-Human chorionic somatomammotropin hormone-like 1 (CSHL1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CSHL1
• Synonyms: CS-5; CSHP1; CSL; GHB4; hCS-L
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109123 sequence for NM_001318 edited (data generated by NextGen Sequencing)
  ATGGAGGAAACGCAGCAGAAATCCAACTTAGAGCTGCTCCACATCTCCCTGCTGCTCATC
  GAGTCGCGGCTGGAGCCCGTGCGGTTCCTCAGGAGTACCTTCACCAACAACCTGGTGTAT
  GACACCTCGGACAGCGATGACTATCACCTCCTAAAGGACCTAGAGGAAGGCATCCAAATG
  CTGATGGGGAGGCTGGAAGACGGCAGCCACCTGACTGGGCAGACCCTCAAGCAGACCTAC
  AGCAAGTTTGACACAAACTCGCACAACCATGACGCACTGCTCAAGAACTACGGGCTGCTC
  CACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGTGCCGC
  TCTGTGGAGGGCAGCTGTGGCTTCTAG
  
  Clone variation with respect to NM_001318.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001318 unedited
  NNTTGTCGAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGCACCCTC
  GGGTCCTGTGGACAGCTCACCTAGCGGCAATGGCTGCAGGCTCCCGGACGTCCCTGCTCC
  TGGCTTTTGCCCTGCTCTGCCTGCCCTGGCTTCAAGAGGCTGACTCTATTCCGACATCCT
  CCAACATGGAGGAAACGCAGCAGAAATCCAACTTAGAGCTGCTCCACATCTCCCTGCTGC
  TCATCGAGTCGCGGCTGGAGCCCGTGCGGTTCCTCAGGAGTACCTTCACCAACAACCTGG
  TGTATGACACCTCGGACAGCGATGACTATCACCTCCTAAAGGACCTAGAGGAAGGCATCC
  AAATGCTGATGGGGAGGCTGGAAGACGGCAGCCACCTGACTGGGCAGACCCTCAAGCAGA
  CCTACAGCAAGTTTGACACAAACTCGCACAACCATGACGCACTGCTCAAGAACTACGGGC
  TGCTCCACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATGGTGCAGT
  GCCGCTCTGTGGAGGGCAGCTGTGGCTTCTAGGGGCCCGCGTGGCATCCTGTGACCCCTC
  CCCAGTGCCTCTCCTGGCCCTGAAGGTGCCACTCCAGTGCCCACCAGCCTTGTCTTAATA
  AAATTAAGTTGTATTGTTTCATCTGAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCG
  GCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCA
  TGCCTCTCCTGGCCCTGNAAAGTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAAN
  TAAGTTGCATCATTTTGTCTGACTANGTGTCCTTCTATATATATGGGTGAGNGGGGGGGG
  GTTTGGGACCAAGGGGNCAGTTGGAANNACACCTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001318 unedited
  TTTAGAGAAACTTACAACTTATTTTATAAAACAAGGCTGGTGGGCACTGGAGTGGCACCT
  TCAGGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGATGCCACGCGGGCCCCTAGAAGCC
  ACAGCTGCCCTCCACAGAGCGGCACTGCACCATGCGCAGGAATGTCTCGACCTTGTCCAT
  GTCCTTCCTGAAGCAGTGGAGCAGCCCGTAGTTCTTGAGCAGTGCGTCATGGTTGTGCGA
  GTTTGTGTCAAACTTGCTGTAGGTCTGCTTGAGGGTCTGCCCAGTCAGGTGGCTGCCGTC
  TTCCAGCCTCCCCATCAGCATTTGGATGCCTTCCTCTAGGTCCTTTAGGAGGTGATAGTC
  ATCGCTGTCCGAGGTGTCATACACCAGGTTGTTGGTGAAGGTACTCCTGAGGAACCGCAC
  GGGCTCCAGCCGCGACTCGATGAGCAGCAGGGAGATGTGGAGCAGCTCTAAGTTGGATTT
  CTGCTGCGTTTCCTCCATGTTGGAGGATGTCGGAATAGAGTCAGCCTCTTGAAGCCAGGG
  CAGGCAGAGCAGGGCAAAAGCCAGGAGCAGGGACGTCCGGGAGCCTGCAGCCATTGCCGC
  TAGGTGAGCTGTCCACAGGACCCTGAGTGGTGCCTGGTGCCGAATTCGCGGCCGCCCTAT
  AGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTG
  CCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCCGGGGTTATTACGACATTTTG
  GAAAATC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001318
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001318.2, NP_001309.3
• RefSeq Size: 661 bp
• RefSeq ORF: 387 bp
• Locus ID: 1444
• Cytogenetics: 17q23.3
• Domains: hormone
• Protein Families: Secreted Protein
• Gene Summary: The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. This particular family member is expressed in placental villi, although it was originally thought to be a pseudogene. In fact, alternative splicing suggests that the majority of the transcripts would be unable to express a secreted protein. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3), also known as CS-1-3N-4-5, has multiple differences in the coding region, but maintains the reading frame, compared to variant 1. The predicted isoform 3 has a shorter N-terminus and lacks a signal sequence, compared to isoform 1. This variant is the predominant transcript.