DPP1 (CTSC) (NM_148170) Human Untagged Clone

CAT#: SC109065

CTSC (untagged)-Human cathepsin C (CTSC), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DPP1
• Synonyms: CPPI; DPP-I; DPP1; DPPI; HMS; JP; JPD; PALS; PDON1; PLS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_148170, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGTGCTGGGCCCTCCTTGCTGCTCGCCGCCCTCCTGCTGCTTCTCTCCGGCGACGGCGCCGTGCGCT
  GCGACACACCTGCCAACTGCACCTATCTTGACCTGCTGGGCACCTGGGTCTTCCAGGTGGGCTCCAGCGG
  TTCCCAGCGCGATGTCAACTGCTCGGTTATGGGACCACAAGAAAAAAAAGTAGTGGTGTACCTTCAGAAG
  CTGGATACAGCATATGATGACCTTGGCAATTCTGGCCATTTCACCATCATTTACAACCAAGGCTTTGAGA
  TTGTGTTGAATGACTACAAGTGGTTTGCCTTTTTTAAGGATGTCACTGATTTTATCAGTCATTTGTTCAT
  GCAGCTGGGAACTGTGGGGATATATGATTTGCCACATCTGAGGAACAAACTGGTTATTAAATAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_148170 unedited
  CTTAGATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGTCTTCACCTCT
  TTTCTCAGCTCCCTGCAGCATGGGTGCTGGGCCCTCCTTGCTGCTCGCCGCCCTCCTGCT
  GCTTCTCTCCGGCGACGGCGCCGTGCGCTGCGACACACCTGCCAACTGCACCTATCTTGA
  CCTGCTGGGCACCTGGGTCTTCCAGGTGGGCTCCAGCGGTTCCCAGCGCGATGTCAACTG
  CTCGGTTATGGGACCACAAGAAAAAAAAGTAGTGGTGTACCTTCAGAAGCTGGATACAGC
  ATATGATGACCTTGGCAATTCTGGCCATTTCACCATCATTTACAACCAAGGCTTTGAGAT
  TGTGTTGAATGACTACAAGTGGTTTGCCTTTTTTAAGGATGTCACTGATTTTATCAGTCA
  TTTGTTCATGCAGCTGGGAACTGTGGGGATATATGATTTGCCACATCTGAGGAACAAACT
  GGTTATTAAATAGAGCATCTGTTGAGGGACTCTTTTAAAACCACAGCCATGAACAGACGT
  TGGGGCTAAGAGACAGAGCAGCCTGCGACAGTGTGGACCTACCTGTAGCAGCTAGCAAAG
  GCCTCTAGCAGCTACAGTCCCTTCTGGAGTCTTTATTTGCATGCAAAATGCAAAGGAGTC
  CTGGTGACCTACCTCCAAGGCAGCTGCCCTCCTGAACACTCCCTTGGAAAACAGTAAACA
  TCATTTTGGAATGTGAACAACCAGAGACTACACAGGAGAAAGGAAAAAAAAAATTCTGAA
  GATGCAAAATCTTGGGTGGCTTCACCGTTCAGTTTTTTAATAAAAGGAACAATNTACANA
  AAAAAAAANAAAAACTCGACTCTAGATTGCNGCCGCGGTCATAGCTGTTTCCTGAACAGA
  TCCCGGNTGGCATNCCTGTGNACCCTNNCCATGCCTNTNCTGGCCCTGNAAGTGCCACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_148170 unedited
  TAACTATGNAACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTTTTTGTATATT
  GTTCCTTTTATTAAAAAACTGAACGGTGAAGCCACCCAAAATTTTGCATCTTCAGAATTT
  TTTTTTTCCTTTCTCCTGTGTAGTCTCTGGTTGTTCACATTCCAAAATGATGTTTACTGT
  TTTCCAAGGGAGTGTTCAGGAGGGCAGCTGCCTTGGAGGTAGGTCACCAGGACTCCTTTG
  CATTTTGCATGCAAATAAAGACTCCAGAAGGGACTGTAGCTGCTAGAGGCCTTTGCTAGC
  TGCTACAGGTAGGTCCACACTGTCGCAGGCTGCTCTGTCTCTTAGCCCCAACGTCTGTTC
  ATGGCTGTGGTTTTAAAAGAGTCCCTCAACAGATGCTCTATTTAATAACCAGTTTGTTCC
  TCAGATGTGGCAAATCATATATCCCCACAGTTCCCAGCTGCATGAACAAATGACTGATAA
  AATCAGTGACATCCTTAAAAAAGGCAAACCACTTGTAGTCATTCAACACAATCTCAAAGC
  CTTGGTTGTAAATGATGGTGAAATGGCCAGAATTGCCAAGGTCATCATATGCTGTATCCA
  GCTTCTGAAGGTACACCACTACTTTTTTTTCTTGTGGTCCCATAACCGAGCAGTTGACAT
  CGCGCTGGGAACCGCTGGAGCCCACCTGGAAGACCCCAGTGCCCAGCAAGTCAAGAATAG
  TGCAGTTGGCAAGTGTGTCGCAACCACGGCGCCGTCGCCGGAAAAAACACCAGGAGGCGG
  CCAGCACCAAGGAGGGCCCACCACCCTGCTGCAGGGAGCTGAAAAAAAAGTGAAGACCTT
  CTGCCCAAATTCCGGCCGCCCTATATTGAGTCGTATACCAAAATTTGACGGTTCACTAAC
  GAGCTCTGCTTATTAGACCTCCAACCTACCACCTACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_148170
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148170.2, NP_680475.1
• RefSeq Size: 6131 bp
• RefSeq ORF: 414 bp
• Locus ID: 1075
• UniProt ID: P53634
• Cytogenetics: 11q14.2
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Lysosome
• Gene Summary: This gene encodes a member of the peptidase C1 family and lysosomal cysteine proteinase that appears to be a central coordinator for activation of many serine proteinases in cells of the immune system. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate heavy and light chains that form a disulfide-linked dimer. A portion of the propeptide acts as an intramolecular chaperone for the folding and stabilization of the mature enzyme. This enzyme requires chloride ions for activity and can degrade glucagon. Defects in the encoded protein have been shown to be a cause of Papillon-Lefevre syndrome, an autosomal recessive disorder characterized by palmoplantar keratosis and periodontitis. [provided by RefSeq, Nov 2015]
  Transcript Variant: This variant (2) differs in the coding sequence and 3' UTR compared to variant 1. This results in an isoform (b) that is much shorter and contains a different C-terminus than isoform a. Isoform b may act as a negative regulator of a stable mature enzyme. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.