FBXL2 (NM_012157) Human Untagged Clone

CAT#: SC108936

FBXL2 (untagged)-Human F-box and leucine-rich repeat protein 2 (FBXL2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FBXL2
• Synonyms: FBL2; FBL3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_012157, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTTTTCTCAAACAATGATGAAGGCCTTATTAACAAAAAGTTACCCAAAGAACTTCTGTTAAGAATAT
  TTTCCTTCTTGGATATAGTAACTTTGTGCCGATGTGCACAGATTTCCAAGGCTTGGAACATCTTAGCCCT
  GGATGGAAGCAACTGGCAAAGAATAGATCTTTTTAACTTTCAAACAGATGTAGAGGGTCGAGTGGTGGAA
  AATATCTCGAAGCGATGCGGTGGATTCCTGAGGAAGCTCAGCTTGCGAGGCTGCATTGGTGTTGGGGATT
  CCTCCTTGAAGACCTTTGCACAGAACTGCCGAAACATTGAACATTTGAACCTCAATGGATGCACAAAAAT
  CACTGACAGCACGTGTTATAGCCTTAGCAGATTCTGTTCCAAGCTGAAACATCTGGATCTGACCTCCTGT
  GTGTCTATTACAAACAGCTCCTTGAAGGGGATCAGTGAGGGCTGCCGAAACCTGGAGTACCTGAACCTCT
  CTTGGTGTGATCAGATCACGAAGGATGGCATCGAGGCACTGGTGCGAGGTTGTCGAGGCCTGAAAGCCCT
  GCTCCTGAGGGGCTGCACACAGTTAGAAGATGAAGCTCTGAAACACATTCAGAATTACTGCCATGAGCTT
  GTGAGCCTCAACTTGCAGTCCTGCTCACGTATCACGGATGAAGGTGTGGTGCAGATATGCAGGGGCTGTC
  ACCGGCTACAGGCTCTCTGCCTTTCGGGTTGCAGCAACCTCACAGATGCCTCTCTTACAGCCCTGGGTTT
  GAACTGTCCGCGACTGCAAATTTTGGAGGCTGCCCGATGCTCCCATTTGACTGACGCAGGTTTTACACTT
  TTAGCTCGGAATTGCCACGAATTGGAGAAGATGGATCTTGAAGAATGCATCCTGATAACCGACAGCACAC
  TCATCCAGCTCTCCATTCACTGTCCTAAACTGCAAGCCCTGAGCCTGTCCCACTGTGAACTCATCACAGA
  TGATGGGATCCTGCACCTGAGCAACAGTACCTGTGGCCATGAGAGGCTGCGGGTACTGGAGTTGGACAAC
  TGCCTCCTCATCACTGATGTGGCCCTGGAACACCTAGAGAACTGCCGAGGCCTGGAGCGCCTCGAGCTGT
  ACGACTGCCAGCAGGTTACCCGTGCAGGCATCAAGCGGATGCGGGCTCAGCTCCCTCATGTCAAAGTCCA
  CGCCTACTTTGCTCCCGTCACCCCACCGACAGCAGTGGCAGGAAGTGGACAGCGACTGTGCAGGTGCTGT
  GTCATTCTCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012157 unedited
  GACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCGCCGGCGCCGTGTGACTTCGG
  GCTGTGGGCTCGCTCGCGGCTCTTCGGCCATGGTTTTCTCAAACAATGATGAAGGCCTTA
  TTAACAAAAAGTTACCCAAAGAACTTCTGTTAAGAATATTTTCCTTCTTGGATATAGTAA
  CTTTGTGCCGATGTGCACAGATTTCCAAGGCTTGGAACATCTTAGCCCTGGATGGAAGCA
  ACTGGCAAAGAATAGATCTTTTTAACTTTCAAACAGATGTAGAGGGTCGAGTGGTGGAAA
  ATATCTCGAAGCGATGCGGTGGATTCCTGAGGAAGCTCAGCTTGCGAGGCTGCATTGGTG
  TTGGGGATTCCTCCTTGAAGACCTTTGCACAGAACTGCCGAAACATTGAACATTTGAACC
  TCAATGGATGCACAAAAATCACTGACAGCACGTGTTATAGCCTTAGCAGATTCTGTTCCA
  AGCTGAAACATCTGGATCTGACCTCCTGTGTGTCTATTACAAACAGCTCCTTGAAGGGGA
  TCAGTGAGGGCTGCCGAAACCTGNAGTACCTGAACCTCTCTTGGTGTGATCAGATCACGA
  AGGATGGCATCGAGGCACTGGTGCGAGGTTGTCGAGGCCTGAAAGCCCTGCTCCTGAGGG
  GCTGCACACAGTTAGAAGATGAAGCTCTGAAACACATTCAGAATTACTGCCATGAGCTTG
  TGAGCCCTCACTTGCAGTCCTGCTCACGTATCACGGATGGAAGGGTGTGTGCANATATGC
  ANGGNGCTGTCACCGGCTACAGGCTCTCTGGCTTTTCGGGNTGCAGCACCTCACAGATGC
  CCTCTCTTAANGCCCTGGGNTTTGAACTGTCCCCGACTGCAAATTTTGGAAGCTGCCCGA
  TGCTCCATTTGACTGACGCAGGTTTTACCTTTTACCTCGGATGCACGAATGGAAAAAATG
  ACTTGAAATGCATCTGTACGCAAGCACTCTTCAGTTCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012157 unedited
  TTGCATGGTTTATCTCATTTTATGAGTCTAAAGGTACCAACACTTACTGATCTGAGTTTT
  AAGTGTGTAAAACTTTTGAGTAGCTATGGTGTAACCTGATTTTTAAGATTTCAAAATAAG
  CTTGAAAAAAAATCACATCCATTTTACATGCATCCATTCATTACTTCTAATTGATTATAC
  TACAGATAACTTAAGCCTTAGTTCTTTCTCTTTAAGGTTCCCCCCAGTTTTACGAGAGGC
  ATGGACATAATTAGCTTTTTTCCTTTAGTAAAAATGTGTTATGTGCTGTAGCATACACCA
  GAGCTTCTACTTTCCAATCAGGCAACACAGACTCCGAGCTGCTTTTGTTTTTGGTCCCTG
  GAGGTGTATATGACAAGTTGACAGAAACAAAAAGGTGAAGACCCTGCTCCACCCAGTATA
  GAGTCCTCTTTTCTTTGGTGTCTCATGGAAACCTATTAACATGCCTTCCACATAAGTCTC
  TATATATAAAACTATNAGGCATTATGAATTAATTTGCAGTACAGTCACTTTGATAAAGTT
  GCTATTTATCTTCAAAGTGGAAAAGTCCTGCTAATCAAAATGGAATTGTGAATTAAAGTA
  AGCCACTGGCTTAAGTATCAGGTAGAAAATCAACAGCTTATTTTTTCTGCTCAGTTTGTG
  CTAACACTGATGGTCTTTTTGAGGAATGCTGAGTATGCCCAAGGTAGGACTTTCGTACCT
  AGGTAGGTACCAATCTCTTAAGAGATCCAGGTTTCTGGCATGGGCCTAGCTGCATTGANA
  CCACTTGTCATGTGTTAACTGACACAAGCCTTTGATTTGATTGTTNACAGCCGATAAAGG
  GGAAACTATCCCAGAGTACTGGCATCCTACAGAGATCTTTACCTGTAATGGCCTTCCCAA
  GGAGATTAACCATGGGNGATGGGGAATCGGCCAGAAGACCTACGCTTCTAAAGAAAGTAG
  CCTATCCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012157
• Insert Size: 2790 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012157.2, NP_036289.2
• RefSeq Size: 2378 bp
• RefSeq ORF: 1272 bp
• Locus ID: 25827
• UniProt ID: Q9UKC9
• Cytogenetics: 3p22.3
• Domains: LRR, F-box, LRR_CC
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.